期刊
ANALYTICA CHIMICA ACTA
卷 885, 期 -, 页码 132-139出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.04.037
关键词
Toehold-mediated DNA strand displacement reaction; Surface-enhanced Raman scattering DNA sensor; Bimetallic nanodendrite; Signal-off sensor
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [2015R1A2A2A01006445, 2013R1A1A3008484]
- National Research Foundation of Korea [2015R1A2A2A01006445, 2013R1A1A3008484] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A simple and sensitive surface enhanced Raman scattering (SERS)-based DNA sensor that utilizes the toehold-mediated DNA displacement reaction as a target-capturing scheme has been demonstrated. For a SERS substrate, Au-Ag bimetallic nanodendrites were electrochemically synthesized and used as a sensor platform. The incorporation of both Ag and Au was employed to simultaneously secure high sensitivity and stability of the substrate. An optimal composition of Ag and Au that satisfied these needs was determined. A double-strand composed of 'a probe DNA (pDNA)' complementary to 'a target DNA (tDNA)' and 'an indicator DNA tagged with a Raman reporter (iDNA)' was conjugated on the substrate. The conjugation made the reporter molecule close to the surface and induced generation of the Raman signal. The tDNA released the pre-hybridized iDNA from the pDNA via toehold-mediated displacement, and the displacement of the iDNA resulted in the decrease of Raman intensity. The variation of percent intensity change was sensitive and linear in the concentration range from 200 fM to 20 nM, and the achieved limit of detection (LOD) was 96.3 fM, superior to those reported in previous studies that adopted different signal taggings based on such as fluorescence and electrochemistry. (C) 2015 Elsevier B.V. All rights reserved.
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