Editorial Material
Biochemistry & Molecular Biology
Charles A. Ettensohn
Summary: The establishment of early polarity in animal embryos is influenced by the dynamic redistribution of the Wnt signaling effector Dishevelled, a process regulated by the cell cycle and the Wnt receptor Frizzled 1.
Article
Pathology
Natalie M. Rizzo, Lynette M. Sholl, Muhammad T. Idrees, John C. Cheville, Sounak Gupta, Kristine M. Cornejo, Hiroshi Miyamoto, Michelle S. Hirsch, Katrina Collins, Andres M. Acosta
Summary: This study compared the genomic landscape of aggressive and nonaggressive testicular Leydig cell tumors (LCTs) and found that aggressive LCTs are characterized by FH inactivation and multiple copy number changes, while nonaggressive LCTs do not exhibit these characteristics.
Article
Biochemistry & Molecular Biology
Win Sen Heng, Shiau-Chuen Cheah
Summary: Decades of research have shown that phytochemical compounds from plant products offer potential benefits in health maintenance and disease treatment, particularly in addressing noncommunicable diseases like lung cancer. The study identified certain phytochemicals that can inhibit the activation of the Wnt/beta-catenin pathway in lung cancer cells, showing anti-proliferative and cytotoxic effects. These findings suggest that phytochemicals could be explored as alternative treatments for targeting cancer stem cells and tumor growth in lung cancer.
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Parasitology
Shiyi Zheng, Yukang Huang, Hongyun Huang, Bin Yu, Ni Zhou, Junhong Wei, Guoqing Pan, Chunfeng Li, Zeyang Zhou
Summary: NbTMP1, a transmembrane protein of Nosema bombycis, was found to be crucial for the proliferation of the pathogen. Downregulation of NbTMP1 expression significantly inhibited the growth of Nosema bombycis, suggesting its potential as a target for selective breeding of resistant silkworm strains.
PARASITES & VECTORS
(2021)
Article
Endocrinology & Metabolism
Kevan C. Herold, Stephen E. Gitelman, Peter A. Gottlieb, Laura A. Knecht, Ralph Raymond, Eleanor L. Ramos
Summary: This study confirms the consistent preservation of beta-cell function through integrated analysis of multiple clinical trials. Teplizumab demonstrates significant improvement in delaying the progression of stage 2 type 1 diabetes to stage 3, with a favorable safety profile characterized by self-limited adverse events.
Article
Oncology
Cen-Shan Lin, Yuyi Liang, Shu-Guang Su, Yin-Li Zheng, Neng Jiang, Jing Zhou, Ying Zhang, Ru Deng, Chun-Hua Wang, Shi-Xun Lu, Yu-Hua Huang, Li-Li Liu, Chao Zhang, Jing-Ping Yun
Summary: The study identified elevated Nup93 expression in HCC tissues, especially in cases with metastasis, and its association with poorer prognosis. Nup93 was found to promote HCC metastasis and proliferation by regulating beta-catenin translocation, while the interaction between Nup93 and beta-catenin, as well as a positive feedback loop involving LEF1 and beta-catenin, further facilitated metastasis and proliferation in HCC. The findings suggest potential therapeutic targets in the LEF1-Nup93-beta-catenin pathway for HCC treatment.
Article
Biotechnology & Applied Microbiology
Chia-Chun Chang, Ching-Ying Wu, Jhao-Guan Ciou, Ching-Wei Wu, Yi-Chen Wang, Hui-Wen Chang, Maw-Sheng Chien, Chienjin Huang
Summary: This study generated a monoclonal antibody specific to the capsid protein of PCV3, which can be used for diagnosis and pathogenesis studies. A novel linear B-cell epitope and a potential nuclear localization signal in the capsid protein were identified. Recombinant proteins self-assembled into virus-like particles (VLPs) with correct conformation and specificity, providing insights for the development of diagnostic tools and potential vaccines for PCV3.
Review
Medicine, General & Internal
Alexandre Lugat, Clement Bailly, Michel Cherel, Caroline Rousseau, Francoise Kraeber-Bodere, Caroline Bodet-Milin, Mickael Bourgeois
Summary: Radioimmunoconjugates have been widely used in nuclear medicine applications for over 30 years. Recent advances in cancer biology have led to the discovery of new molecular targets specific to certain patient subgroups, allowing for the development of specifically tailored therapeutic approaches. Immuno-positron emission tomography (immuno-PET) is a non-invasive imaging modality that utilizes monoclonal antibodies for in vivo characterization of tumor biomarkers, providing valuable information on tumor targeting. Immuno-PET shows great potential in various nuclear medicine applications, including staging/stratification, theranostic approaches, and predictive/prognostic biomarkers. Finding the right balance between isotope choice and immunologic structure is crucial for the development of radiopharmaceutical drugs for immuno-PET.
FRONTIERS IN MEDICINE
(2022)
Article
Cell Biology
Heidi Espadas-Alvarez, Jacqueline Martinez-Rendon, Isabel Larre, Arturo Matamoros-Volante, Tatiana Romero-Garcia, Tamara Rosenbaum, Angelica Rueda, Refugio Garcia-Villegas
Summary: TRPV4 channel functions in the nucleus, inhibiting the transcriptional activity of beta-catenin through a direct interaction, and may regulate differentiation in various cellular contexts.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Megan Wagstaff, Brandon Coke, Georgia R. Hodgkiss, Rhys G. Morgan
Summary: Dysregulation of 13-catenin in the Wnt signaling pathway is implicated in the emergence, maintenance, and drug resistance of leukemia stem cells in AML. Despite its therapeutic potential, 13-catenin inhibitors have not been used in the treatment of AML.
BIOSCIENCE REPORTS
(2022)
Article
Multidisciplinary Sciences
Fanlin Li, Huihui Zhang, Wanting Wang, Puyuan Yang, Yue Huang, Junshi Zhang, Yaping Yan, Yuan Wang, Xizhong Ding, Jie Liang, Xinyue Qi, Min Li, Ping Han, Xiaoqing Zhang, Xin Wang, Jiang Cao, Yang-Xin Fu, Xuanming Yang
Summary: The study presents a selective CAR-T cell therapy that can eradicate T cell malignancies while maintaining functional T cell-mediated immune responses. This research is of great importance for the eradication of T cell malignancies and opens up new possibilities for clinical development.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Lisa Hiepp, Doris Mayr, Kathrin Gaertner, Elisa Schmoeckel, Frederick Klauschen, Alexander Burges, Sven Mahner, Reinhard Zeidler, Bastian Czogalla
Summary: Targeting the tumor-associated carbonic anhydrase XII (CA XII) is considered a promising strategy to improve cancer treatment. This study provides deeper information about the expression profile of CA XII in ovarian carcinomas, revealing significant expression in most tumor samples and ascites-derived cells. Higher levels of CA XII were found in ovarian carcinomas compared to borderline tumors and non-neoplastic ovarian epithelia. High expression of CA XII was associated with higher tumor grading and a trend towards shorter overall survival. The results suggest that CA XII plays a crucial role in the malignancy of ovarian carcinoma cells and has potential as a diagnostic marker and therapeutic target.
Article
Immunology
Adam Mcnee, Daryll Vanover, Pramila Rijal, Basudev Paudyal, Fabian Z. X. Lean, Ronan Macloughlin, Alejandro Nunez, Alain Townsend, Philip J. Santangelo, Elma Tchilian
Summary: Monoclonal antibodies are a potential therapy for influenza virus infection. In a pig model of influenza, a strongly neutralizing monoclonal antibody called 2-12C was found to reduce virus load and lung pathology, but did not prevent virus nasal shedding.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Xueying Yang, Fei Shao, Dong Guo, Wei Wang, Juhong Wang, Rongxuan Zhu, Yibo Gao, Jie He, Zhimin Lu
Summary: FTO plays a critical role in cancer development by removing m(6)A modifications from genes. Downregulation of FTO expression in lung adenocarcinoma patients is associated with poor survival. Mechanistically, Wnt signaling induces EZH2 binding to beta-catenin, inhibiting FTO expression and promoting m(6)A modifications on specific genes like MYC to enhance tumor cell glycolysis and growth.
CELL DEATH & DISEASE
(2021)
Article
Physiology
Seok-Jo Kim, Paul Cheresh, Renea P. Jablonski, Lyudmila Rachek, Anjana Yeldandi, Raul Piseaux-Aillon, Mark J. Ciesielski, Karen Ridge, Cara Gottardi, Anna P. Lam, Annie Pardo, Moises Selman, Viswanathan Natarajan, David W. Kamp
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Paul Cheresh, Seok-Jo Kim, Long Shuang Huang, Satoshi Watanabe, Nikita Joshi, Kinola J. N. Williams, Monica Chi, Ziyan Lu, Anantha Harijith, Anjana Yeldandi, Anna P. Lam, Cara Gottardi, Alexander Misharin, G. R. Scott Budinger, Viswanathan Natarajan, David W. Kamp
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Editorial Material
Cell Biology
Cara J. Gottardi, G. W. Gant Luxton
JOURNAL OF CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Rogan A. Grant, Luisa Morales-Nebreda, Nikolay S. Markov, Suchitra Swaminathan, Melissa Querrey, Estefany R. Guzman, Darryl A. Abbott, Helen K. Donnelly, Alvaro Donayre, Isaac A. Goldberg, Zasu M. Klug, Nicole Borkowski, Ziyan Lu, Hermon Kihshen, Yuliya Politanska, Lango Sichizya, Mengjia Kang, Ali Shilatifard, Chao Qi, Jon W. Lomasney, A. Christine Argento, Jacqueline M. Kruser, Elizabeth S. Malsin, Chiagozie O. Pickens, Sean B. Smith, James M. Walter, Anna E. Pawlowski, Daniel Schneider, Prasanth Nannapaneni, Hiam Abdala-Valencia, Ankit Bharat, Cara J. Gottardi, G. R. Scott Budinger, Alexander Misharin, Benjamin D. Singer, Richard G. Wunderink
Summary: The study found that patients infected with SARS-CoV-2 have enriched T cells and monocytes in the alveolar space, suggesting that the virus infects alveolar macrophages and induces T cell production of interferon-, leading to inflammation and persistent alveolitis.
Article
Medicine, Research & Experimental
Alexandra C. McQuattie-Pimentel, Ziyou Ren, Nikita Joshi, Satoshi Watanabe, Thomas Stoeger, Monica Chi, Ziyan Lu, Lango Sichizya, Raul Piseaux Aillon, Ching- Chen, Saul Soberanes, Zhangying Chen, Paul A. Reyfman, James M. Walter, Kishore R. Anekalla, Jennifer M. Davis, Kathryn A. Helmin, Constance E. Runyan, Hiam Abdala-Valencia, Kiwon Nam, Angelo Y. Meliton, Deborah R. Winter, Richard Morimoto, Gokhan M. Mutlu, Ankit Bharat, Harris Perlman, Cara J. Gottardi, Karen M. Ridge, Navdeep S. Chandel, Jacob Sznajder, William E. Balch, Benjamin D. Singer, Alexander Misharin, G. R. Scott Budinger
Summary: This study identified homogeneous age-related changes in the transcriptome of alveolar macrophages and demonstrated that the lung microenvironment affects the proliferation capacity of these cells, which in turn impacts the resistance of older patients to influenza viral infection. Targeting strategies towards the aging lung microenvironment may be necessary to restore alveolar macrophage function in aging individuals.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Satoshi Watanabe, Nikolay S. Markov, Ziyan Lu, Raul Piseaux Aillon, Saul Soberanes, Constance E. Runyan, Ziyou Ren, Rogan A. Grant, Mariana Maciel, Hiam Abdala-Valencia, Yuliya Politanska, Kiwon Nam, Lango Sichizya, Hermon G. Kihshen, Nikita Joshi, Alexandra C. McQuattie-Pimentel, Katherine A. Gruner, Manu Jain, Jacob I. Sznajder, Richard I. Morimoto, Paul A. Reyfman, Cara J. Gottardi, G. R. Scott Budinger, Alexander V. Misharin
Summary: This study revealed that promoting the differentiation of AT2 cells into AT1 cells with ISRIB can accelerate epithelial repair, reduce the recruitment of profibrotic alveolar macrophages, and ameliorate lung fibrosis in the pathogenesis of pulmonary fibrosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Anthea Weng, Mariana Maciel Herrerias, Satoshi Watanabe, Lynn C. Welch, Annette S. Flozak, Rogan A. Grant, Raul Piseaux Aillon, Laura A. Dada, Seung Hye Han, Monique Hinchcliff, Alexander Misharin, G. R. Scott Budinger, Cara J. Gottardi
Summary: This study reveals that lung injury leads to hypertrophy and polyploidy of alveolar epithelial cells. The small molecule ISRIB can attenuate the hypertrophic and polyploid features of these cells. These findings suggest a potential link between alveolar epithelial injury and hypertrophic growth and polyploidization of the cells.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paul A. Reyfman, Elizabeth S. Malsin, Basil Khuder, Nikita Joshi, Gaurav Gadhvi, Annette S. Flozak, Mary A. Carns, Kathleen Aren, Isaac A. Goldberg, Seokjo Kim, Michael Alexander, Peter H. S. Sporn, Alexander Misharin, G. R. Scott Budinger, Anna P. Lam, Monique Hinchcliff, Cara J. Gottardi, Deborah R. Winter
Summary: Tissue availability is a limitation of single-cell genomic technologies in studying cellular heterogeneity. This study performed single-cell RNA sequencing on 40,000 cells from the BAL of healthy volunteers, identifying six cell types and confirming the expression of marker genes. A subpopulation of macrophages expressing genes associated with MIP-1 was characterized. This study provides insights into lung macrophage heterogeneity and serves as a resource for future research.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Che-Fan Huang, Cara J. Gottardi, Milan Mrksich
Summary: Phosphorylation plays a crucial role in cellular signaling, and dysregulation of phosphorylation can lead to diseases. However, while most studies focus on kinases, the role of phosphatases is often overlooked. This study demonstrates that basic amino acids adjacent to phosphotyrosines can restrict the activity of tyrosine phosphatases, providing insights into the mechanisms underlying disease-associated mutations and potential treatment strategies.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Anthea Weng, Erik E. Rabin, Annette S. Flozak, Sergio E. Chiarella, Raul Piseaux Aillon, Cara J. Gottardi
Summary: The adherens junction component, alpha-T-catenin (alpha Tcat), plays an important role in the junction structure of cardiomyocytes and in cell-cell junctions in the nervous system. Recent genomic studies have linked CTNNA3 polymorphisms to various diseases, suggesting its involvement in maintaining neuroglial functions of the central and peripheral nervous systems.
Article
Medicine, Research & Experimental
Laura A. Dada, Lynn C. Welch, Natalia D. Magnani, Ziyou Ren, Hyebin Han, Patricia L. Brazee, Diego Celli, Annette S. Flozak, Anthea Weng, Mariana Maciel Herrerias, Vitalii Kryvenko, Istvan Vadasz, Constance E. Runyan, Hiam Abdala-Valencia, Masahiko Shigemura, S. Marina Casalino-Matsuda, Alexander Misharin, G. R. Scott Budinger, Cara J. Gottardi, Jacob I. Sznajder
Summary: Persistent symptoms and radiographic abnormalities suggestive of failed lung repair are common in COVID-19 patients after hospital discharge. Hypercapnia, which often occurs in mechanically ventilated patients, limits the proliferative capacity of alveolar type II cells and impairs lung repair after injury. Understanding the role of hypercapnia in lung repair is important for preventing complications and improving patient outcomes.
Editorial Material
Cell Biology
Robert M. Harmon, Cara J. Gottardi
Summary: Desmosomes and keratin have been found to regulate the distribution and dynamics of the endoplasmic reticulum (ER). This suggests that this intercellular adhesive and cytoskeletal network may coordinate a range of ER functions.
NATURE CELL BIOLOGY
(2023)
Meeting Abstract
Critical Care Medicine
D. R. Winter, G. Gadhvil, E. Malsin, M. Carnsl, K. Arent, I. A. Goldberg, A. Lam, C. J. Gottardi
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)
Meeting Abstract
Critical Care Medicine
P. A. Reyfman, E. Malsin, M. J. Alexander, A. Flozak, S. Kim, A. Misharin, P. H. Sporn, A. Lam, M. Hinchcliff, G. Budinger, C. J. Gottardi, D. R. Winter
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)
Meeting Abstract
Endocrinology & Metabolism
R. Assaloni, A. Michelli, B. Brunato, E. Barro, E. Manca, A. Petrucco, C. Gottardi, S. Cum, A. Da Porto, R. Candido, R. Da Ros
DIABETES TECHNOLOGY & THERAPEUTICS
(2020)