期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 144, 期 1-3, 页码 854-864出版社
HUMANA PRESS INC
DOI: 10.1007/s12011-011-9031-z
关键词
Mercury; Chelator; Monoclonal antibody; Immunoassay; Competitive indirect ELISA
资金
- National 863 Project of China [2007AA10Z440]
- National Natural Science Foundation of China [30972224, 31072178, 30871897]
Immunoassays for heavy metals offer an alternative approach to traditional techniques for detection of mercury. In this study, a mercury-chelate was prepared with 1-(4-aminobenzyl) ethylenediamine-N,N,N',N'-tetraacetic acid (aminobenzyl-EDTA). The resulting complex was linked to keyhole limpet hemocyanin (KLH) or bovine serum albumin via the amino group and used as the immunizing antigen or detection antigen, respectively. BALB/c mice were immunized with KLH-aminobenzyl-EDTA-Hg and spleen cells from BALB/C mice were fused with Sp2/0 cells. One cell line (5F7) produced monoclonal antibodies with preferential selectivity and sensitivity for aminobenzyl-EDTA-Hg. This cell line had an affinity constant of 4.31 x 10(9) L/mol and its cross-reactivity (CR) with other metals was < 2%. The antibody was used for competitive indirect ELISA (CI-ELISA) for Hg(2+) measurements. The detection range was 0.087-790.4 mu g/L and the lower limit of detection was 0.042 mu g/L. The concentrations of mercury in environmental water samples obtained by CI-ELISA correlated well with graphite furnace atomic absorption spectrometry (GFAAS), and the mean recovery was 88.82% to 104.64%. These results indicate that this method could be used for monitoring mercury of water.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据