Article
Biotechnology & Applied Microbiology
Maryam Yousefvand, Behrouz Harighi, Abdolbaset Azizi
Summary: In this study, the effects of volatile organic compounds (VOCs) produced by endophytic bacteria belonging to the Bacillus genus on the virulence traits of Ralstonia solanacearum R32 were investigated. Gas chromatography-mass spectrometry analysis revealed that different strains of the Bacillus genus produced a range of VOCs. The VOCs produced by the bacteria significantly reduced wilting symptoms, inhibited the growth of R. solanacearum R32, and affected various aspects of the pathogenicity, such as motility and biofilm formation.
BIOLOGICAL CONTROL
(2023)
Article
Plant Sciences
Kambiz Bahmani, Nader Hasanzadeh, Behrouz Harighi, Alireza Marefat
Summary: This study aimed to isolate endophytic bacteria from potato plants to investigate their antagonistic effects on Ralstonia solanacearum. Out of the 236 isolated bacteria, 31 showed potential antagonistic effects, with Pseudomonas brassicacearum Psb101, P. brassicacearum Ps169, Paenibacillus peoriae Pa86, Pseudomonas putida Ps52, and Bacillus licheniformis Bl17 exhibiting the greatest inhibitory effects. Additionally, all 11 selected isolates were effective in reducing disease and enhancing plant growth, particularly the five mentioned strains.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Microbiology
Yaxing Su, Yanan Xu, Hailing Liang, Gaoqing Yuan, Xiaogang Wu, Dehong Zheng
Summary: This study identified key genes required for R. solanacearum survival in tomato plants using transposon insertion sequencing technology, highlighting the importance of genes related to cell wall/membrane/envelope biogenesis, amino acid transport and metabolism, energy production and conversion, posttranslational modification, protein turnover, and chaperones in the pathogenic mechanism of this bacterium.
Article
Agriculture, Multidisciplinary
Keke Dang, Jinfeng Hou, Hong Liu, Junwei Peng, Yang Sun, Jiangang Li, Yuanhua Dong
Summary: In this study, the changes in rhizobacteria, endobacteria, and root exudates during the pathogenesis of bacterial wilt in ginger caused by Ralstonia solanacearum (Rs) were analyzed using high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). The results showed that Rs infection led to a 50.00% incidence of bacterial wilt in ginger and altered the composition of bacterial communities in both the endosphere and rhizosphere. Furthermore, the changes in rhizobacterial composition induced corresponding changes in endobacterial and root exudate compositions. The upregulated exudates inhibited ginger bacterial wilt, indicating that ginger produces antibacterial compounds as a defense mechanism against bacterial pathogens.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Yiting Li, Gaofu Qi, Ziqiong Xie, Baolong Li, Rui Wang, Jun Tan, Heli Shi, Bikun Xiang, Xiuyun Zhao
Summary: Bacterial wilt disease causes heavy yield losses in crops, and endophytic microbiomes play an important role in controlling plant diseases. This study investigates the differences in endophytic root microbiomes and network structures between healthy and diseased tobacco plants. The results show that the composition and network structures of endophytic root microbiomes are distinct between healthy and diseased plants. Diseased plants have a lower abundance of beneficial bacteria and lack beneficial keystone microorganisms, leading to an unstable network.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Peipei Qi, Mengling Huang, Xuehan Hu, Ying Zhang, Ying Wang, Pengyue Li, Shiyun Chen, Dan Zhang, Sen Cao, Wanting Zhu, Jiatao Xie, Jiasen Cheng, Yanping Fu, Daohong Jiang, Xiao Yu, Bo Li
Summary: The type III effector RipAB of Ralstonia solanacearum directly interacts with TGAs in Arabidopsis, interfering with the recruitment of RNA Pol II at TGA-regulons and disrupting immune gene expression.
Article
Ecology
Mei Li, Thomas Pommier, Yue Yin, Jianing Wang, Shaohua Gu, Alexandre Jousset, Joost Keuskamp, Honggui Wang, Zhong Wei, Yangchun Xu, Qirong Shen, George A. Kowalchuk
Summary: The rhizosphere microbiome plays a crucial role in defending against soilborne pathogens, and inhibiting the growth of pathogen helpers may be effective in reducing pathogen densities. This approach could potentially become a sustainable strategy for pathogen control.
Article
Soil Science
Lv Su, Huatai Li, Jing Wang, Wenting Gao, Xia Shu, Xiting Sun, Kesu Wang, Yan Duan, Yunpeng Liu, Eiko E. Kuramae, Ruifu Zhang, Biao Shen
Summary: Bacteria that dominate and become enriched in the rhizosphere during continuous cropping have attracted increasing interest due to their ability to adapt well to the rhizosphere. However, little is known about their general composition and function. In this study, we conducted high-throughput sequencing and culture-dependent workflows to investigate the composition and function of rhizosphere bacteria during continuous cropping of tomatoes in different soils. We observed a similar succession of bacterial community compositions in the rhizosphere, and identified certain bacteria that were gradually enriched and potentially beneficial. Our study also revealed the enrichment of predicted functions related to xenobiotic biodegradation, nutrient metabolism, and antibiotic biosynthesis in different rhizosphere soils. We further demonstrated the inhibitory effect of Beijerinckia fluminensis GR2, which was gradually enriched in all tested soils, on the growth of Ralstonia solanacearum and its protective role against infection. These findings provide new insights into the assembly mechanism of gradually enriched bacteria and their role as plant-beneficial microbes in the rhizosphere.
BIOLOGY AND FERTILITY OF SOILS
(2023)
Article
Biotechnology & Applied Microbiology
Jianlei Shi, Deju Shui, Shiwen Su, Zili Xiong, Wenshan Zai
Summary: In this study, the transcriptomes of resistant and susceptible tomato lines under control and inoculated conditions were analyzed, and several key genotype-specific hub genes involved in different biological processes were identified. These findings lay a foundation for a better understanding of the molecular basis by which resistant tomato lines respond to Ralstonia solanacearum.
Article
Biochemistry & Molecular Biology
Ansar Hussain, Liu Kaisheng, Ali Noman, Muhammad Furqan Ashraf, Mohammed Albaqami, Muhammad Ifnan Khan, Zhiqin Liu, Shuilin He
Summary: In this study, the functional role of CaASHH3 protein in plant immunity was characterized in pepper. It was found that CaASHH3 acts as a positive regulator in cell death and pepper immunity against bacterial pathogens, and its function is regulated by signals mediated by SA, JA, ET, and ABA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ankita Pandey, Hayoung Moon, Sera Choi, Hayeon Yoon, Maxim Prokchorchik, Jay Jayaraman, Rajendran Sujeevan, Yu Mi Kang, Honour C. McCann, Cecile Segonzac, Chul Min Kim, Soon Ju Park, Kee Hoon Sohn
Summary: In this study, the R. solanacearum effector RipJ was identified as playing a pathogenic role in a specific eggplant species and triggering bacterial wilt resistance. Despite belonging to the YopJ family of acetyltransferases, RipJ does not require conserved residues in the acetyltransferase catalytic triad to induce immunity.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Microbiology
Honghong Dong, Xin Xu, Ruixiang Gao, Yueqiu Li, Anzhang Li, Qing Yao, Honghui Zhu
Summary: This study reports the discovery of a myxobacteria strain, Myxococcus xanthus R31, that exhibits high antagonistic activity against the pathogenic bacterium Ralstonia solanacearum. The study reveals that the strain can efficiently predate and secrete lytic proteins to control tomato bacterial wilt.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Entomology
Wenguang Shi, Jiaqi Li, Huaxiong Yao, Jianbin Li, Meiqi Chen, Danting Chen, Yinghao Fu, Huarong Xie, Zujun Deng
Summary: In this study, the diversity of endophytic fungi in healthy patchouli plants was analyzed using Illumina sequencing. Forty isolates of Myrothecium were obtained from patchouli roots and one strain (SWG1) showed the most potent activity against RS in vitro. In pot experiments, the inoculation of SWG1 significantly increased the growth of patchouli seedlings and reduced the incidence and severity of patchouli RS wilt.
Article
Microbiology
Jianming Huang, Zhangjiang He, Jiankang Wang, Xingping Zha, Qing Xiao, Guihua Liu, Yongjie Li, Jichuan Kang
Summary: This study identifies an effector, FlSp1, from Fusarium lateritium, which acts as an immune-triggering protein by stimulating ROS accumulation in the plant immune system to limit fungal proliferation and balance the interaction between the endophytic fungi and their host plants.
Review
Microbiology
Kenji Kai
Summary: Ralstonia solanacearum species complex (RSSC) strains are devastating plant pathogens distributed worldwide. The primary cell density-dependent gene expression system in RSSC strains is phc quorum sensing (QS), which regulates the expression of about 30% of all genes. The specificity in generating and receiving its QS signal, but their signaling pathways might not differ significantly. This review discusses the genetic and biochemical factors involved in QS signal input, the regulatory network, and the interactions with soil fungi.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xinshen Li, Xiaomei Huang, Gongyou Chen, Lifang Zou, Lingen Wei, Juling Hua
Article
Biochemical Research Methods
Xingshen Li, Tingting Bai, Yunfeng Li, Xiaolei Ruan, Huaping Li
Editorial Material
Plant Sciences
Xinshen Li, Rong Zeng, Xiaomei Huang, Jian Chen, Wensheng Fang, Aocheng Cao, Juling Hua
