期刊
BIOLOGICAL CHEMISTRY
卷 395, 期 12, 页码 1389-1399出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2014-0204
关键词
crystallization; membrane protein complex; Na+ transport; NADH: quinone oxidoreductase; respiratory chain; Vibrio cholerae
资金
- Baden-Wurttemberg Stiftung [P-LS-Meth/4]
- Deutsche Forschungsgemeinschaft [FR 1321/3-1, FR 1488/3-2]
Vibrio cholerae is a Gram-negative bacterium that lives in brackish or sea water environments. Strains of V. cholerae carrying the pathogenicity islands infect the human gut and cause the fatal disease cholera. Vibrio cholerae maintains a Na+ gradient at its cytoplasmic membrane that drives substrate uptake, motility, and efflux of antibiotics. Here, we summarize the major Na+-dependent transport processes and describe the central role of the Na+-translocating NADH: quinone oxidoreductase (Na+-NQR), a primary Na+ pump, in maintaining a Na+-motive force. The Na+-NQR is a membrane protein complex with a mass of about 220 kDa that couples the exergonic oxidation of NADH to the transport of Na+ across the cytoplasmic membrane. We describe the molecular architecture of this respiratory complex and summarize the findings how electron transport might be coupled to Na+-translocation. Moreover, recent advances in the determination of the three-dimensional structure of this complex are reported.
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