Article
Pharmacology & Pharmacy
Claire C. Munier, Christian Ottmann, Matthew W. D. Perry
Summary: The 14-3-3 proteins play crucial roles in regulating the inflammatory response at genetic, molecular, and cellular levels. They affect key components of the immune response and can lead to clinical syndromes when their recognition processes are disrupted. Abnormal levels of 14-3-3 contribute to undesirable immune responses and chronic inflammatory conditions.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hetao Chen, Hang Zhang, Pu Chen, Song Xiang
Summary: This study elucidated the interaction mechanism between CRTCs and 14-3-3, as well as their crucial roles in nuclear translocation and activation of gene transcription. The discovery of a novel salt bridge structure expanded the understanding of this interaction.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chang-Heng Hsieh, Chia-Cheng Chou, Ya-Ching Fang, Po-Hao Hsu, Yi-Hung Chiu, Chi-Sheng Yang, Guey-Mei Jow, Chih-Yung Tang, Chung-Jiuan Jeng
Summary: Endogenous 14-3-3 proteins play a chaperone-like role in regulating the stability of Eag1 protein and modulators of 14-3-3 have therapeutic potential in correcting protein expression of disease-causing Eag1 mutants.
CELL AND BIOSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Stephen I. Ting, Dylan W. Snelson, Tucker R. Huffman, Akihiro Kuroo, Ryota Sato, Ryan A. Shenvi
Summary: Researchers have reported a concise synthesis of (-)-cotylenol via a 10-step asymmetric entry. This route features a reaction that tolerates steric hindrance and a tandem reaction that establishes an 8-membered ring. The method sets the stage for focused library synthesis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Plant Sciences
Hiroshi Fukayama, Fumihiro Miyagawa, Naoki Shibatani, Aiko Koudou, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Yasuo Yamauchi, Daisuke Matsuoka, Ryutaro Morita
Summary: CO2-responsive CCT protein (CRCT) functions as a positive regulator of starch synthesis-related genes, specifically in the leaf sheath of rice. It binds to the promoter regions of these genes, forms complexes in vivo, and interacts with 14-3-3-like proteins to control the expression of key starch synthesis-related genes, despite differences in expression sites.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Cell Biology
Jia-Hua Qu, Kirill Tarasov, Khalid Chakir, Yelena S. Tarasova, Daniel R. Riordon, Edward G. Lakatta
Summary: This study investigated the structure and function of the cardiac 14-3-3 protein interactome, revealing that the proteins bound to 14-3-3 are mainly related to mitochondria and play a crucial role in regulating cardiac metabolism and proteostasis.
Article
Plant Sciences
Heidi Pertl-Obermeyer, Ana Gimeno, Verena Kuchler, Evrim Servili, Shuai Huang, Han Fang, Veronika Lang, Katharina Sydow, Magdalena Poeckl, Waltraud X. Schulze, Gerhard Obermeyer
Summary: Pollen tube growth and fertilization are precisely regulated processes involving cellular and molecular events, such as the activity and phosphorylation regulation of the plasma membrane H+ ATPase. Phosphorylation and pH modulation significantly influence the activity of H+ ATPase, while the metabolic components have minimal effects. Local regulation of H+ ATPase activity generates localized H+ influxes and effluxes, as well as pH gradients in the pollen tube tip, instead of heterogeneous localized distribution in the plasma membrane.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Wei Jiang, Jing He, Mohammad Babla, Ting Wu, Tao Tong, Adeel Riaz, Fanrong Zeng, Yuan Qin, Guang Chen, Fenglin Deng, Zhong-Hua Chen
Summary: This review comprehensively explores the roles of 14-3-3 proteins and H+-ATPases in plant cell signaling and their potential in enhancing plant stress tolerance. The molecular evolution of these proteins in green plants is analyzed, and their stress-specific expression in different plant species is discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Yue Han, Haiqiong Ye, Ping Li, Yifan Zeng, Jing Yang, Meng Gao, Zhengding Su, Yongqi Huang
Summary: Tau, a major microtubule-associated protein, can form liquid droplets. 14-3-3 zeta protein can be recruited into tau droplets and regulate their formation through electrostatic and hydrophobic interactions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Bente A. Somsen, Fenna W. B. Craenmehr, Wei-Hong W. Liu, Auke A. Koops, Marloes A. M. Pennings, Emira J. Visser, Christian Ottmann, Peter J. Cossar, Luc Brunsveld
Summary: Molecular glues represent a novel approach in drug discovery, however, the targeted stabilization of protein complexes is still challenging due to a lack of drug design rules. This study demonstrates the successful development of a peptide-based molecular glue that selectively stabilizes the 14-3-3/ChREBP protein-protein interaction by utilizing the functional mapping of hotspots.
Article
Fisheries
Xiaoyi Pan, Qiang Gao, Jinyu Shen, Ting Xu
Summary: This study identified Mr14-3-3 as a binding protein for MrTV-VP3, playing a crucial role in MrTV infection and potentially serving as a target for anti-MrTV therapies.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Physiology
Christopher Solis, Walter C. C. Thompson, James R. R. Pena, Christopher McDermott-Roe, Paulina Langa, Chad M. M. Warren, Magdalena Chrzanowska, Beata M. M. Wolska, R. John Solaro, Pieter H. Detombe, Paul Goldspink
Summary: In this study, the function and regulation mechanism of MGF's E-domain were investigated. By analyzing the sequence and protein interactions, it was found that the phosphorylation state regulates the stability of 14-3-3 proteins and their interactions with relevant proteins. This has important implications for understanding the regulation of cardiac function.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Xianlong Huang, Zhiwen Zheng, Yixin Wu, Meng Gao, Zhengding Su, Yongqi Huang
Summary: 14-3-3 proteins are important scaffold proteins that are widely expressed in various tissues. They interact with numerous protein targets and mediate multiple cellular signaling pathways. Recent studies have suggested that 14-3-3 proteins may play a significant role in liquid-liquid phase separation (LLPS). By modulating the conformation and recruiting other molecules, 14-3-3 proteins can efficiently regulate the functions of their targets in the context of LLPS.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Dmitri Segal, Stefan Maier, Giovanni J. Mastromarco, Wesley Wei Qian, Syed Nabeel-Shah, Hyunmin Lee, Gaelen Moore, Jessica Lacoste, Brett Larsen, Zhen-Yuan Lin, Abeeshan Selvabaskaran, Karen Liu, Craig Smibert, Zhaolei Zhang, Jack Greenblatt, Jian Peng, Hyun O. Lee, Anne-Claude Gingras, Mikko Taipale
Summary: This study maps the interactomes of all human 14-3-3 paralogs and characterizes their effect on client protein localization. It reveals that 14-3-3 proteins function as chaperone-like molecules and negatively regulate the localization and activity of certain proteins. These findings reshape our understanding of the function of 14-3-3 proteins.
Article
Mechanics
Yadong Han, Lei Tan
Summary: In this study, a closed-loop experimental rig with high-speed observation is established for a mixed flow pump, and numerical simulation on tip leakage cavitation is conducted with experimental validation. A new double-hump pattern of tip leakage cavitation is observed for the first time in the mixed flow pump. The spatial-temporal evolution of tip leakage cavitation with double-hump can be classified into four stages: incepting stage, growing stage, merging stage, and propagating stage.
Article
Plant Sciences
Heidi Pertl-Obermeyer, Ana Gimeno, Verena Kuchler, Evrim Servili, Shuai Huang, Han Fang, Veronika Lang, Katharina Sydow, Magdalena Poeckl, Waltraud X. Schulze, Gerhard Obermeyer
Summary: Pollen tube growth and fertilization are precisely regulated processes involving cellular and molecular events, such as the activity and phosphorylation regulation of the plasma membrane H+ ATPase. Phosphorylation and pH modulation significantly influence the activity of H+ ATPase, while the metabolic components have minimal effects. Local regulation of H+ ATPase activity generates localized H+ influxes and effluxes, as well as pH gradients in the pollen tube tip, instead of heterogeneous localized distribution in the plasma membrane.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biochemical Research Methods
Ipek Ergal, Guenther Bochmann, Werner Fuchs, Simon K-M R. Rittmann
Summary: The metabolic diversity of organisms in natural microbial ecosystems enables interaction and syntrophic interactions among community members. Artificial microbial consortium engineering is used in biotechnology to improve productivity and yields. However, to achieve supreme productivity or efficiency at industrial scale, defined ecosystems must be physiologically well-selected to meet eco-biotechnological demands.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Franziska Steger, Johanna Reich, Werner Fuchs, Simon K. -M. R. Rittmann, Georg M. Guebitz, Doris Ribitsch, Guenther Bochmann
Summary: Strategies for depleting carbon dioxide (CO2) from flue gases are urgently needed, and carbonic anhydrases (CAs) can contribute to solving this problem. This current study examines four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab), and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA and PmCA. AwCA showed high stability in a mesophilic temperature range, while PmCA proved to be exceptionally thermostable. These results indicate the potential to utilize CAs from anaerobic microorganisms for CO2 sequestration applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Nikola Hanisakova, Monika Vitezova, Simon K. -M. R. Rittmann
Summary: The cultivation and investigation of strictly anaerobic microorganisms is important in the fields of anaerobic microbial physiology, microbiology, and biotechnology. Anaerobic cultivation methods differ from classic microbiological techniques in terms of instrument requirements and challenges. Anaerobic microbiological methods play a crucial role in the isolation and characterization of new species, physiological examination, anaerobic biotechnological applications, and medical indications.
Article
Microbiology
Matthew Gavino Donadu, Marco Ferrari, Vittorio Mazzarello, Stefania Zanetti, Ivan Kushkevych, Simon K. -M. R. Rittmann, Anette Stajer, Zoltan Barath, Dora Szabo, Edit Urban, Mario Gajdacs
Summary: Biofilm formation is crucial for the survival and protection of Staphylococcus spp. in vivo. This study assessed the interdependence between biofilm-forming capacity and antibiotic-resistant phenotype in 299 environmental Staphylococcus spp. isolates. The results showed no significant association between biofilm formation and methicillin resistance, but a significant association with rifampicin susceptibility.
Article
Energy & Fuels
Franziska Steger, Ipek Ergal, Armin Daubek, Nadine Loibl, Lydia Rachbauer, Werner Fuchs, Simon K. -M. R. Rittmann, Guenther Bochmann
Summary: In this study, the reproducibility of trickle-bed bioreactors for acetic acid production from H-2:CO2 was evaluated, showing that higher gas and medium flow rates can increase acetic acid production rates. Initial glucose addition resulted in unwanted butyric acid accumulation, while omitting glucose led to higher acetic acid yields. The acetogenic trickle-bed bioreactors were found to be an effective solution for converting H-2/CO2 to acetate using an anaerobic enrichment culture.
FRONTIERS IN ENERGY RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Kevin Pfeifer, Eva-Kathrin Ehmoser, Simon K-M R. Rittmann, Christa Schleper, Dietmar Pum, Uwe B. Sleytr, Bernhard Schuster
Summary: The outermost component of cell envelopes in most bacteria and almost all archaea is a protein lattice called Surface (S-) layer, which has a highly porous structure with regularly arranged pores in the nm-range. The S-layer fragments of a hyperthermophilic acidophilic archaeon SSO show high retention efficiency and reduced extraction costs, making them economically attractive.
Article
Multidisciplinary Sciences
Thiago Rodrigues-Oliveira, Florian Wollweber, Rafael I. Ponce-Toledo, Jingwei Xu, Simon K. -M. R. Rittmann, Andreas Klingl, Martin Pilhofer, Christa Schleper
Summary: Asgard archaea are close relatives of eukaryotes and their genomes contain hundreds of eukaryotic signature proteins (ESPs), which play a role in the formation of cytoskeleton and cellular structures. By studying a highly enriched culture of 'Candidatus Lokiarchaeum ossiferum', researchers found that this Asgard archaea has a larger genome and possesses a complex actin-based cytoskeleton, suggesting that such cytoskeleton existed prior to the emergence of eukaryotes.
Article
Microbiology
Hayk Palabikyan, Aquilla Ruddyard, Lara Pomper, David Novak, Barbara Reischl, Simon K. -M. R. Rittmann
Summary: This study provides a comprehensive revision and optimization of cultivation methods for Methanococcus maripaludis, a model organism for the production of methane biofuel. The research successfully achieved scale-up from serum bottles to a stainless steel bioreactor, contributing to the establishment of methanogens as essential organisms in large-scale biotechnology applications.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Microbiology
Zhe Lyu, Amelia-Elena Rotaru, Mark Pimentel, Cui-Jing Zhang, Simon K. -M. R. Rittmann
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Michael Melcher, Logan H. Hodgskiss, Mohammad Anas Mardini, Christa Schleper, Simon K. -M. R. Rittmann
Summary: This study investigated the biomass productivity and physiological response of Nitrososphaera viennensis to different ammonium and carbon dioxide concentrations. The results showed that increasing CO2 concentration in continuous culture can enhance growth rate and biomass yield. Moreover, the formation of biofilm and changes in ammonium concentration affected the reliability of nitrite concentration as a proxy for cell number in continuous culture. These findings have important implications for the biomass production and yield of ammonia-oxidizing archaea (AOA).
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Ruth-Sophie Taubner, Lydia M. F. Baumann, Michael Steiner, Kevin Pfeifer, Barbara Reischl, Kordian Korynt, Thorsten Bauersachs, Barbara Maehnert, Elisabeth L. Clifford, Joern Peckmann, Bernhard Schuster, Daniel Birgel, Simon K. -M. R. Rittmann
Summary: Methanogenic archaea are important anaerobic microorganisms with diverse metabolic characteristics. This study provides a comprehensive quantitative analysis of the lipidome and proteinogenic amino acid excretion patterns of three autotrophic methanogens under different temperatures and nutrient supplies. The results show that each methanogen has unique patterns and rates of amino acid excretion and lipidome modulation, which can be influenced by temperature and substrate concentration. Additionally, water production rate is higher than methane production rate for all studied methanogens. This research highlights the need for quantitative comparative physiological studies to understand microbial responses to environmental conditions.
Review
Microbiology
Werner Fuchs, Lydia Rachbauer, Simon K. -M. R. Rittmann, Guenther Bochmann, Doris Ribitsch, Franziska Steger
Summary: Biotechnology has the potential to significantly contribute to a low-carbon society through various green processes. The authors identify eight promising biotechnology tools that can be game changers, either by utilizing the unique properties of living cells or through new scientific advancements. These tools include the Wood-Ljungdahl pathway, carbonic anhydrase, cutinase, methanogens, electro-microbiology, hydrogenase, cellulosome, and nitrogenase. The authors provide an overview of the latest research and practical implementation of these tools, explaining why they are considered game changers.
Article
Biotechnology & Applied Microbiology
Martin Koller, Simon K. -M. R. Rittmann
Summary: Haloarchaea, an extremely halophilic branch of Archaea, has attracted attention as microbial cell factories for producing valuable products like polyhydroxyalkanoates (PHA). However, there are still technical challenges to overcome before industrial-scale production can be achieved.
CURRENT RESEARCH IN BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lydia M. F. Baumann, Ruth-Sophie Taubner, Kinga Olah, Ann-Cathrin Rohrweber, Bernhard Schuster, Daniel Birgel, Simon K. -M. R. Rittmann
Summary: This study demonstrates the high biotechnological potential of archaeal lipids, particularly the utilization of Methanothermobacter marburgensis as a carbon dioxide based methane producer and potential supplier of archaeal lipids. Different cultivation conditions have a significant impact on the production of specific core lipids, and up-scaling at a constant particle number shows promise for industrial applications.
BIOENGINEERING-BASEL
(2022)
