Article
Environmental Sciences
Mario Corrochano-Monsalve, Carmen Gonzalez-Murua, Adrian Bozal-Leorri, Luis Lezama, Benat Artetxe
Summary: The study unveiled the mechanism of action of nitrification inhibitors with copper elements, demonstrating the effects of 3,4-dimethyl-1H-pyrazole dihydrogen phosphate and a new DMP-based nitrification inhibitor. Despite their differences, both compounds showed the same nitrification inhibition efficiency in soils with varying copper content, suggesting that DMP plays an active role in both cases.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Physical
Yusaku Kodama, Shinya Ariyasu, Masayuki Karasawa, Yuichiro Aiba, Osami Shoji
Summary: In this study, researchers achieved highly regioselective hydroxylation of alkanes at the terminal position by combining CYP153A33 with substrate analog decoy molecules. This combination method not only worked effectively for propane, but also for ethane and methane.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Vasiliy M. Muzalevskiy, Alexey V. Shastin, Sarvinoz I. Tirkasheva, Odiljon E. Ziyadullaev, Askar B. Parmanov, Valentine G. Nenajdenko
Summary: A novel catalytic system for homocoupling terminal acetylenes was developed based on CuCl as a catalyst, TMEDA as a base, and CCl4 as an oxidant. The reaction was studied in terms of solvent, base, amount of catalyst, and CCl4. Methanol was found to be the preferred solvent, and the reaction showed a wide synthetic scope with yields up to 92% for diynes with various substituents. The possible reaction mechanism was discussed.
Article
Chemistry, Physical
Yusaku Kodama, Shinya Ariyasu, Masayuki Karasawa, Yuichiro Aiba, Osami Shoji
Summary: Regioselectivities in the direct hydroxylation of C-H bonds by cytochrome P450s depend on the C-H bond-dissociation energies. Combining CYP153A33 with substrate analog decoy molecules can alter the enzyme's substrate specificity, enabling selective hydroxylation of alkanes at the terminal position.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anurag Noonikara-Poyil, Alvaro Munoz-Castro, H. V. Rasika Dias
Summary: This study describes the isolation and full characterization of a thermally stable, copper(I) acetylene complex using a highly fluorinated bis(pyrazolyl)borate ligand support, as well as the details of a related copper(I) complex. Raman data show significant red-shifts in C equivalent to C stretch of different alkynes bound to copper(I), with computational analysis indicating that the interaction is primarily of the electrostatic character. The study also highlights the catalytic activity of a mononuclear copper complex in [3 + 2] cycloadditions and compares its effectiveness with a trinuclear copper catalyst.
Article
Chemistry, Physical
Soren Brander, Radina Tokin, Johan O. Ipsen, Poul Erik Jensen, Cristina Hernandez-Rollan, Morten H. H. Norholm, Leila Lo Leggio, Paul Dupree, Katja S. Johansen
Summary: Lytic polysaccharide monooxygenases (LPMOs) are copper enzymes that work with glycoside hydrolases to break down polysaccharides. While O-2 and H2O2 can both be used as cosubstrates for LPMOs, the Lentinus similis LPMO (LsAA9A) specifically cleaves cellooligomers with H2O2 as a cosubstrate. The reaction mechanism of LsAA9A suggests that the scission of glucosidic bonds and oxidation of saccharides are not directly coupled.
Review
Biology
Hina Ayub, Min-Ju Kang, Adeel Farooq, Man-Young Jung
Summary: The interactions between metals and microbes play a critical role in geomicrobiology and microbial ecophysiological processes. This review provides insight into the physiological pathways of aerobic ammonia and methane oxidation by different microbial groups, highlighting the role of metalloenzymes. The study emphasizes the importance of iron and copper concentrations in ecosystems, as they can impact the activity and growth of methane-oxidizing bacteria and ammonia-oxidizing microorganisms, ultimately influencing global carbon and nitrogen cycles.
Review
Chemistry, Applied
Rui Zhu, Yang Liu, Yueying Yang, Qing Min, Hua Li, Lixia Chen
Summary: Steroids are a class of drugs with diverse pharmacological properties. Hydroxylation of steroids significantly affects their biological activities and properties. Achieving regio- and stereo-selective hydroxylation on steroids using modern organic synthesis techniques is extremely challenging. However, recent studies have identified and engineered cytochrome P450 monooxygenases that possess the ability to catalyze regio- and stereo-selective oxidations of nonactivated C-H bonds in complex organic molecules.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Article
Environmental Sciences
Wen Chen, Sifu Li, Dingyi Bai, Zongfang Li, Haozhe Liu, Lianyang Bai, Lang Pan
Summary: The excessive use of chemical herbicides has led to the evolution of herbicide-resistant weeds. Cytochrome P450 monooxygenases (P450s) play a vital role in detoxification of herbicide-resistant weeds. A resistant population of Polypogon fugax (R population) was found to be resistant to quizalofop-p-ethyl, pyroxsulam, and several other herbicides. Molecular analysis revealed no target-site gene mutations in the R population. The overexpression of the CYP89A2 gene was found to be responsible for reducing quizalofop-p-ethyl phytotoxicity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Chemistry, Multidisciplinary
Guoliang Zhu, Hui Zeng, Shuangbo Zhang, Jana Juli, Linhua Tai, Danyang Zhang, Xiaoyun Pang, Yan Zhang, Sin Man Lam, Yun Zhu, Guohong Peng, Hartmut Michel, Fei Sun
Summary: The study elucidated the structure of a hyperthermophilic bacterium cytochrome c oxidase, revealing a unique dimeric structure essential for naphthoquinol binding. This suggests an evolutionary adaptation of the oxidase to its hyperthermophilic environment, shedding light on the structural variation of cytochrome c oxidases in different species.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Jin Chang, Peng Peng, Muhammad Farhan Ul-Haque, Abid Hira, Alan A. DiSpirito, Jeremy D. Semrau
Summary: Copper plays a critical role in controlling greenhouse gas emissions, and methanobactin (MB) secreted by methanotrophs can limit the ability of other microbes to gather copper, thereby impacting microbial community composition. This study shows that different forms of MB increased nitrous oxide (N2O) production and caused significant shifts in microbial community composition in forest soils. The effects of MB were strongest in low-copper soils, and MB-SB2 had a stronger effect due to its higher affinity for copper. The presence of MB also inhibited nitrite reduction and altered the presence of specific genes involved in denitrification.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Environmental Sciences
Leanne Sims, Chloe Wright, Andrew T. Crombie, Robin Dawson, Colin Lockwood, Nick E. Le Brun, Laura Lehtovirta-Morley, J. Colin Murrell
Summary: The study investigated the co-oxidation of various alkenes, dienes, and aromatic compounds by whole cells of the isoprene-degrading bacterium expressing isoprene monooxygenase. The results revealed the broad substrate specificity of this monooxygenase and identified 1-octyne as a potential inhibitor for distinguishing isoprene consumption by bona fide isoprene degraders and co-oxidation of isoprene by other oxygenase-containing bacteria.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Arsenij Kokorin, Pavel D. Parshin, Patrick J. Bakkes, Anastasia A. Pometun, Vladimir Tishkov, Vlada B. Urlacher
Summary: The fusion of multiple enzymes as multifunctional constructs has been shown to improve enzymatic properties, with noticeable increases in substrate conversion and activity observed. The enhanced diflavin reductase activity of P450 BM3 was identified as a key factor contributing to the improved performance of the fusion constructs.
SCIENTIFIC REPORTS
(2021)
Review
Microbiology
Lars Hederstedt
Summary: This review article discusses the role and protein components involved in the assembly of cytochrome c oxidase in animals, plants, and aerobic bacteria. The study focuses on the analysis of mutants and comparative biochemistry to understand the assembly process and its implications for disease and antimicrobial drug development.
Article
Biotechnology & Applied Microbiology
Anton A. Stepnov, Zarah Forsberg, Morten Sorlie, Giang-Son Nguyen, Alexander Wentzel, Asmund K. Rohr, Vincent G. H. Eijsink
Summary: The study describes a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, and investigates the effects of the reductant and copper ions on the LPMO reaction. Results show that ascorbate-driven LPMO reactions are extremely sensitive to very low amounts of free copper, whereas reactions driven by gallic acid are almost unaffected by the presence of free copper ions.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
