Article
Biochemistry & Molecular Biology
Rajakumar Selvaraj, Sarah Zehnder, Russell Watts, Jihong Lian, Chinmayee Das, Randal Nelson, Richard Lehner
Summary: Two distinct diacylglycerol acyltransferases (DGAT1 and DGAT2) catalyze the final step of TG synthesis in hepatocytes. TG is either stored in cytosolic LDs or assembled into VLDLs in the ER. DGAT1-made TG is stored in smaller LDs while DGAT2-made TG is stored in larger LDs. ATGL targets DGAT1-generated LDs and the fatty acids released from these LDs are preferentially used for oxidation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Review
Biochemistry & Molecular Biology
Guanqun Chen, John L. Harwood, M. Joanne Lemieux, Scot J. Stone, Randall J. Weselake
Summary: This review comprehensively discusses research advances on DGATs in prokaryotes and eukaryotes with a focus on their biochemical properties, physiological roles, and biotechnological and therapeutic applications.
PROGRESS IN LIPID RESEARCH
(2022)
Article
Multidisciplinary Sciences
Xuewu Sui, Kun Wang, Kangkang Song, Chen Xu, Jiunn Song, Chia-Wei Lee, Maofu Liao, Robert V. Farese, Tobias C. Walther
Summary: This study reports the structures of inhibitors of the triacylglycerol synthesis enzyme. The inhibitors were found to block the enzyme's activity by either blocking the entrance of the substrate binding tunnel or interacting with the catalytic residues. Furthermore, the inhibitors showed minimal activity against related enzymes, but a single-residue mutation increased their inhibitory effect.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Hongli Cui, Chunchao Zhao, Wenxin Xu, Hongjiang Zhang, Wei Hang, Xiaoli Zhu, Chunli Ji, Jinai Xue, Chunhui Zhang, Runzhi Li
Summary: The study identified HpDGAT2A, HpDGAT2D, and HpDGAT2E genes in Haematococcus lacustris that showed significant increases in expression levels under high light and nitrogen deficient conditions, leading to substantial TAG and EAST accumulation. These genes were able to restore TAG synthesis in yeast cells and exhibited preferences for monounsaturated and polyunsaturated fatty acids.
Article
Biochemistry & Molecular Biology
Scot J. Stone
Summary: This article reviews the contribution of enzymes involved in glycerol 3-phosphate and monoacylglycerol acyltransferase pathways to intestinal triacylglycerol metabolism, and briefly discusses the potential of some of these enzymes as therapeutic targets for treating metabolic disorders associated with elevated triacylglycerol.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Biotechnology & Applied Microbiology
Lin Zhang, Song Wang, Ji-Chang Han, Guan-Pin Yang, Ke-Hou Pan, Ji-Lin Xu
Summary: The study demonstrates that overexpression of AtDGAT in Nannochloropsis significantly increases lipid and TAG content without compromising algal growth. Enhancing TAG content through genetic engineering is a feasible and effective strategy for commercial biofuel production. This is the first successful attempt of plant-derived DGAT gene overexpression in Nannochloropsis, bringing novel insights into genetic manipulation of oleaginous microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Plant Sciences
Jay Shockey, Prasad Parchuri, Gregory N. Thyssen, Philip D. Bates
Summary: The chemical and physical properties of vegetable oils are determined by the ratios of fatty acids they contain. Some plant species can accumulate unusual fatty acids in large amounts. Cotton is one such example and produces cyclopropyl fatty acids which have industrial applications. In this study, cotton acyltransferases were cloned and characterized to understand their role in cyclopropyl fatty acid accumulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yu Wang, Fansi Zeng, Zheng Zhao, Lin He, Xiaohong He, Huimin Pang, Feifei Huang, Pingan Chang
Summary: TMEM68 functions as an acyltransferase and affects lipogenic gene expression, glycerolipid metabolism and TG storage in mammalian cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Jikun Song, Wenfeng Pei, Nuohan Wang, Jianjiang Ma, Yue Xin, Shuxian Yang, Wei Wang, Quanjia Chen, Jinfa Zhang, Jiwen Yu, Man Wu, Yanying Qu
Summary: By comparative transcriptome analysis, this study reveals the gene expression network involved in oil synthesis in cottonseeds and proposes the main pathway for oil production. Additionally, GhCYSD1 is identified as a potential key player in oil biosynthesis.
PHYSIOLOGIA PLANTARUM
(2022)
Review
Pharmacology & Pharmacy
Neeta B. Amin, Aditi R. Saxena, Veena Somayaji, Robert Dullea
Summary: DGAT1 and DGAT2 are two different enzymes that differ significantly in multiple aspects. Clinical studies of the DGAT2 inhibitor ervogastat suggest that it has good efficacy and tolerability in patients with NAFLD and NASH, making it an effective therapeutic strategy for these patients.
CLINICAL THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Xin Shen, Xinyi Liang, Xiaoguo Ji, Jiangshan You, Xinye Zhuang, Yudong Song, Hao Yin, Mengyao Zhao, Liming Zhao
Summary: The study revealed that chitobiose (GlcN)2 and chitotriose (GlcN)3 have inhibitory effects on lipid accumulation under a non-high-fat diet, by reducing the expression of TG synthesis and fatty acid uptake-related signaling pathways. Additionally, overexpression of CD36 and DGAT2 genes showed promising therapeutic potential in regulating hepatic steatosis.
Article
Biochemistry & Molecular Biology
Pamela J. McFie, Paulos Chumala, George S. Katselis, Scot J. Stone
Summary: The study utilized CRISPR/Cas9 gene editing to add three consecutive FLAG epitopes to the C-terminus of endogenous DGAT2 in HepG2 cells, allowing successful detection of the protein at its endogenous locus within the cell.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biotechnology & Applied Microbiology
Paeka Klaitong, Akaraphol Watcharawipas, Sirirat Fa-aroonsawat, Wipa Chungjatupornchai
Summary: This study investigated the regulation of diacylglycerol acyltransferase 2 gene (NeoDGAT2) in microalgae by analyzing the regulatory sequence RDG, revealing the potential role of MYB transcription factor in controlling TAG biosynthesis. The interaction between MYB transcription factor and MYB-bs may play a crucial role in regulating -N induced expression of NeoDGAT2 and affecting TAG accumulation, making MYB transcription factors a potential target for engineering to increase TAG content for economic viability.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yohei Ishibashi, Shohei Sadamitsu, Yoshitomo Fukahori, Yuki Yamamoto, Rin Tanogashira, Takashi Watanabe, Masahiro Hayashi, Makoto Ito, Nozomu Okino
Summary: Thraustochytrids are marine microorganisms that synthesize and store useful lipids. This study identified DGAT2C as the sterol ester synthase and DGAT2D as the triglyceride synthase in Aurantiochytrium limacinum. DGAT2C is localized to the endoplasmic reticulum and its suitable N-terminal deletion can increase sterol production.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Kyeong-Ryeol Lee, Yong Min Kim, Yumi Yeo, Semi Kim, Mi Chung Suh
Summary: In this study, DGAT3 was found to contribute to the accumulation of seed storage oil and preferred the acylation of C18:3. Overexpression of CsDGAT3-2 or CsDGAT3-3 led to increased seed weight and total fatty acid levels in transgenic Arabidopsis seeds, especially in C18:3 and C20:1 levels. Knockout of DGAT3 in camelina resulted in reduced seed weight and fatty acid levels, particularly in C18:3 and C20:1. There were no significant differences in total seed fatty acid levels or composition between the Arabidopsis dgat1 mutant and the dgat1 mutant overexpressing CsDGAT3-3.
INDUSTRIAL CROPS AND PRODUCTS
(2022)