期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 33, 期 7, 页码 1117-1121出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.33.1117
关键词
L-type amino acid transporter; cancer cell; cell cycle regulatory factor; 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid
资金
- Ministry of Education, Science and Technology [KRF-2008-313-E00551]
The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at Cl phase by inhibiting cyclin D3 CDK6 complex whereas increasing expression of a CDK inhibitor p27.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据