Article
Microbiology
Stephanie J. Ruiz, Joury S. van't Klooster, Frans Bianchi, Bert Poolman
Summary: Amino acids are essential for metabolism but can be toxic at high levels intracellularly. Downregulation of amino acid transporters in Saccharomyces cerevisiae is a mechanism to avoid toxicity. Overexpression of certain amino acid transporters can induce growth defects, and various amino acids are growth inhibitory to yeast cells when transported into the cell at high levels.
Article
Chemistry, Applied
Cemile Yilmaz, Senel Ecem Berk, Vural Gokmen
Summary: The effects of environmental stresses on the formation of amino acid derivatives by Saccharomyces cerevisiae NCYC 88 and Saccharomyces cerevisiae NCYC 79 were investigated. Yeasts were more affected by osmotic, temperature, and alcohol stresses. Both yeast strains increased the formation of kynurenic acid, tryptophan ethyl ester, tryptophol, and gamma-aminobutyric acid under osmotic stress conditions. The results may enable the development of new strategies for designing novel foods with a desired composition of bioactive amino acid derivatives.
Article
Biotechnology & Applied Microbiology
Andreea Perpelea, Andy Wiranata Wijaya, Luis C. Martins, Dorthe Rippert, Mathias Klein, Angel Angelov, Kaisa Peltonen, Attila Teleki, Wolfgang Liebl, Peter Richard, Johan M. Thevelein, Ralf Takors, Isabel Sa-Correia, Elke Nevoigt
Summary: This study successfully demonstrated the co-fermentation of D-GalUA and glycerol by introducing the fungal D-GalUA pathway into yeast strain S. cerevisiae, resulting in the production of ethanol at a high rate in synthetic minimal medium. The fast and complete consumption of D-GalUA in crude sugar beet pulp hydrolysate was also confirmed, indicating potential for industrial application.
METABOLIC ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Zhiyong Li, Yajuan Fu, Jinyu Shen, Jiansheng Liang
Summary: WNK8 gene expression is post-transcriptionally regulated through an upstream open reading frame (uORF) in its 5' untranslated region, causing ribosome stalling. WNK8 and its downstream target RACK1 synergistically coordinate ABA signaling rather than antagonistically modulating glucose response and flowering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Yoichi Toyokawa, Jirasin Koonthongkaew, Hiroshi Takagi
Summary: BCAT catalyzes transamination between BCAAs and BCKAs in the cell. Eukaryotic cells have mitochondrial BCAT (BCATm) and cytosolic BCAT (BCATc) isozymes, which have similar enzymatic functions but differences in physiological functions and regulatory mechanisms. The variations of BCAT could have significant implications for research into the relationship between their primary structures and protein functions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yukio Watanabe, Wataru Aoki, Mitsuyoshi Ueda
Summary: Producing ammonia outside of yeast cells through cell surface engineering is an efficient approach to avoid growth deficiencies caused by ammonia assimilation gene knockout. Displaying HcLAAO on yeast cell surfaces successfully produced ammonia with a conversion efficiency higher than previous studies. This study demonstrates the promising strategy of utilizing food processing wastes for ammonia production.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Cheng Cheng, Wei-Bin Wang, Meng-Lin Sun, Rui-Qi Tang, Long Bai, Hal S. Alper, Xin-Qing Zhao
Summary: This study reveals that the NGG1 gene in yeast plays a regulatory role in xylose metabolism, and its deletion can improve xylose consumption. Further analysis shows that the deletion of NGG1 also affects mitochondrial function, ATP biosynthesis, and amino acid accumulation. These findings provide a basis for developing yeast strains for the production of ethanol and biochemicals from lignocellulosic biomass.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Chunxiao Yan, Ning Gao, Xuan Cao, Lun Yao, Yongjin J. Zhou, Jiaoqi Gao
Summary: This study found that the deficiency of amino acids and nucleotides has effects on cell growth and product synthesis in yeast. These effects can be restored by adding appropriate supplementation of amino acids or nucleotides.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Green & Sustainable Science & Technology
Marianna Dourou, Christina N. Economou, Lida Aggeli, Miroslav Janak, Gabriela Valdes, Nefeli Elezi, Dimitrios Kakavas, Theodore Papageorgiou, Alexandra Lianou, Dimitrios V. Vayenas, Milan Certik, George Aggelis
Summary: Pomegranate residues can be used as a potential feedstock for chemical and biotechnological processes. The polysaccharides extracted from PRs can be used as substrate for biotechnological applications, including ethanol and single cell oil production.
JOURNAL OF CLEANER PRODUCTION
(2021)
Review
Plant Sciences
Yong Feng, Mengyun Jiang, Weichang Yu, Jiannan Zhou
Summary: The increasing recognition of the roles of short/small open reading frames (sORFs) in recent years is attributed to the development and application of the Ribo-Seq technique, which allows for the identification of sORFs in various organisms. However, caution should be exercised when using RPFs to identify sORFs in plants, particularly for polyploidy species, due to the small size and complexity of the plant genome. This study compares different approaches to the identification of plant sORFs, discusses their advantages and disadvantages, and provides a guide for researchers in choosing the appropriate method for plant sORF studies.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Josimar M. Batista, Maria J. Neves, Helvecio C. Menezes, Zenilda L. Cardeal
Summary: A new amino acid extraction method using functionalized magnetic carbon nanotubes was developed and optimized, which showed excellent extraction of analytes. Exposure of Saccharomyces cerevisiae to BaP at a concentration of 25.0 μmol L-1 caused changes in cell viability, indicating metabolic alterations. A fast and efficient GC/MS method was optimized to determine 16 amino acids in yeasts exposed or not to BaP. Comparison of amino acid concentrations revealed statistically significant differences in glycine, serine, phenylalanine, proline, asparagine, aspartic acid, glutamic acid, tyrosine, and leucine, suggesting their potential as toxicity biomarker candidates.
Article
Biotechnology & Applied Microbiology
Dameng Yang, Wencheng Su, Yingying Jiang, Shushan Gao, Xiangying Li, Ge Qu, Zhoutong Sun
Summary: This study established a platform using engineered brewer's yeast for the synthesis of various β-lactam nuclei, allowing access to structurally different antibiotics. By integrating heterologous biosynthetic pathways and designed enzymes, the researchers successfully produced valuable antibiotic intermediates, demonstrating the significance of this work for antibiotic production.
METABOLIC ENGINEERING
(2022)
Article
Microbiology
Guohua Zhang, Qianhui Qi, Faizan Ahmed Sadiq, W. Wang, Xiaxia He, Wei Wang
Summary: The study focused on the protein changes in sourdough fermented with single culture or mixed culture, showing that carbohydrate metabolism is crucial for sourdough quality. Greater abundance of certain enzymes contributing to sourdough quality was observed in mixed-culture-based sourdough. However, some compounds important for enhancing nutritional characteristics and flavor of sourdough decreased in abundance in the mixed-culture-based sourdough, which might affect the taste and texture of the sourdough.
Article
Multidisciplinary Sciences
Yu Chen, Jens Nielsen
Summary: The resource costs for protein synthesis, specifically the biosynthesis of amino acids, have a strong influence on the relative abundances of amino acids in the proteome of Saccharomyces cerevisiae. The research shows that S. cerevisiae tends to minimize protein resource, rather than glucose or energy, for synthesizing amino acids.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Salome Nashed, Houssam El Barbry, Medine Benchouaia, Angelie Dijoux-Marechal, Thierry Delaveau, Nadia Ruiz-Gutierrez, Lucie Gaulier, Deborah Tribouillard-Tanvier, Guillaume Chevreux, Stephane Le Crom, Benoit Palancade, Frederic Devaux, Elodie Laine, Mathilde Garcia
Summary: Mitochondria play a crucial role in eukaryotic cells, and defects in their biogenesis are associated with various human diseases. Through systematic analysis of yeast amino acid preferences, researchers discovered a strong and specific overrepresentation of hydrophobic residues at position 2 of mitochondrial targeting sequences (MTS), which is conserved throughout evolution. Using CRISPR/Cas9 technology, they confirmed the importance of the residue at position 2 in mitochondrial protein import. These findings have valuable implications for improving the treatment of human mitochondrial diseases.
