期刊
BIOLOGIA PLANTARUM
卷 52, 期 1, 页码 146-148出版社
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-008-0030-7
关键词
shoot culture; copper; zinc; manganese; phytoremediation
Ailanthus altissima, a fast-growing and contamination-resistant species is investigated for its use in areas contaminated by heavy metals. A micropropagation protocol for A. altissima was developed, cultured shoots were tested for in vitro heavy metals tolerance. Proliferation rate and shoot length were affected by 6-benzylaminopurine (BAP) and Murashige and Skoog's (MS) salt concentrations, best results were obtained in full strength MS medium supplemented with 1.32 or 2.64 mu M BAP. Rooting percentage was strongly influenced by indole-3-butyric acid. Cultures of A. altissima exposed to heavy metals demonstrated a tolerance comparable to species already utilized in phytoremediation.
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