Article
Multidisciplinary Sciences
Yi-xiao Liu, Francisco J. Chaparro, Ziting Tian, Yizhen Jia, John Gosser, Jeremy Gaumer, Liam Ross, Hooman Tafreshi, John J. Lannutti
Summary: We used laser metrology to study the effect of collector rotation on the porosity gradients of electrospun PCL scaffolds. It was found that the porosity varied with rotational speed, with higher rotational speeds leading to lower porosity. The relatively small changes in porosity values resulted in large variations in pore size, which affected cell infiltration. Faster rotational speeds improved axial alignment but decreased pore size, compromising cell infiltration. Sacrificial fiber approaches were also found to be inefficient in achieving cell-permissive pore sizes.
Article
Engineering, Biomedical
Haifeng Liu, Yuqing Wang, Yi Yang, Anqing Wang, Chongquan Huang, Zhijun Zhao, Ping Li, Meili Liu, Yubo Fan
Summary: This study developed aligned conductive scaffolds made of graphene and silk fibroin, with mechanical and electrochemical properties improving as graphene content increased, but declining when graphene concentration exceeded 4%. Cell experiments showed that aligned G/SF scaffolds were not neurotoxic to primary spinal cord neurons and promoted neurite elongation through upregulation of Netrin-1 expression. Among all scaffolds tested, the A3% G/SF scaffold demonstrated optimal mechanical and electrochemical performance along with enhanced neurite outgrowth, making it a promising candidate for electrically active tissue scaffolding in neural regeneration or engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Multidisciplinary Sciences
K. Dhillon, K. Aizel, T. J. Broomhall, E. Secret, T. Goodman, M. Rotherham, N. Telling, J. M. Siaugue, C. Menager, J. Fresnais, M. Coppey, A. J. El Haj, M. A. Gates
Summary: This study presents a novel approach to control the directional growth of neuronal outgrowth using magnetic nanoparticles and a magnetic array. The results show the viability of this approach in both in vitro and ex vivo models, and highlight its potential for developing new therapies for Parkinson's disease.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Engineering, Biomedical
Li Liu, Mengyu Chen, Junming Zhang, Haobo Li, Zhaoxia Li, Jianhao Song, Shutao Ma, Yingjie Wang, Xiangxin Lou
Summary: The present study used oriented nanofiber scaffolds to mimic the microenvironment of SGNs and promote neurite outgrowth. The results showed that the scaffolds can enhance SGN growth, with the 2% PANI/PLLA/Gel group exhibiting the best effects. These findings provide a feasible research direction for SGN regeneration and the treatment of SNHL in the future.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Materials Science, Biomaterials
Jiaqi Fang, Xuehan Jin, Bo Xu, Liping Nan, Shuhao Liu, Jianguang Wang, Na Niu, Zhong Wu, Feng Chen, Junjian Liu
Summary: Chlorogenic acid (CGA) is a polyphenol with high bioactivity and therapeutic potential for various diseases. This study investigated the role of CGA in promoting peripheral nerve regeneration. A biodegradable conduit containing CGA-releasing microspheres was successfully prepared and used for repairing sciatic nerve defects, demonstrating favorable effects on functional recovery.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Biomaterials
Christopher J. Brown, Thomas Simon, Chiara Cilibrasi, Peter J. Lynch, Rhiannon W. Harries, Aline Amorim Graf, Matthew J. Large, Sean P. Ogilvie, Jonathan P. Salvage, Alan B. Dalton, Georgios Giamas, Alice A. K. King
Summary: This study has developed a scaffold with good biocompatibility and mechanical properties that can promote cell proliferation. Additionally, it was found that this scaffold has a unique growth-promoting effect on an aggressive form of brain cancer in experiments without the addition of growth factors.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Oleksandr Gryshkov, Fedaa Al Halabi, Antonia Isabel Kuhn, Sara Leal-Marin, Lena Julie Freund, Maria Forthmann, Nils Meier, Sven-Alexander Barker, Kirsten Haastert-Talini, Birgit Glasmacher
Summary: PVDF and P(VDF-TrFE) are promising biomaterials for nerve regeneration, with piezoelectric properties stimulating cell growth. The study compared their structural and piezoelectric properties, using a dynamic impact test machine. PVDF showed increased beta-phase content post-electrospinning and similar zeta potential to P(VDF-TrFE), while also demonstrating guiding properties for nerve outgrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lena Mungenast, Fabian Zueger, Jasmin Selvi, Ana Bela Faia-Torres, Juergen Ruehe, Laura Suter-Dick, Maurizio R. Gullo
Summary: This study introduces a cost-effective gelatin-based submicron patterned hydrogel-fiber composite with tunable stiffness, which can support cell attachment, differentiation, and alignment of neurons. The scaffold combines enzymatically crosslinked gelatin-based hydrogels with electrospun poly(epsilon-caprolactone) (PCL) alignment cues to optimize neuron attachment, proliferation, and alignment. This technology is robust, inexpensive, and suitable for neural tissue engineering requiring directional neuron alignment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tienli Ma, Shangchih Yang, Shyhchyang Luo, Weili Chen, Shulang Liao, Weifang Su
Summary: This paper presents dual-function fibrous scaffolds that promote cell attachment and viability. The scaffolds are made from a co-polypeptide with lysine enhancing cell adhesion and glutamate improving cell viability. Neural cells grown on these scaffolds exhibit better activity and longer neurite length. Additionally, when the scaffolds are hydrolyzed, cell viability and neurite growth further increase.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Anna Liguori, Elisabetta Petri, Chiara Gualandi, Luisa S. Dolci, Valentina Marassi, Mauro Petretta, Andrea Zattoni, Barbara Roda, Brunella Grigolo, Eleonora Olivotto, Francesco Grassi, Maria Letizia Focarete
Summary: The incorporation of H2S slow-release donors inside biomimetic silk fibroin-based electrospun scaffolds can open up new possibilities in tissue regeneration and anti-inflammatory treatment. Due to the instability of the donor in the solvent needed for silk fibroin solutions, a multilayer structure was created with a PLGA mat containing the donor sandwiched between two silk fibroin nanofibrous layers.
Article
Materials Science, Multidisciplinary
Erfan Rezvani Ghomi, Rajamani Lakshminarayanan, Vijila Chellappan, Navin Kumar Verma, Amutha Chinnappan, Rasoul Esmaeely Neisiany, Kottaiswamy Amuthavalli, Zhi Sheng Poh, Brandon Han Siang Wong, Nileshkumar Dubey, Roger Narayan, Seeram Ramakrishna
Summary: This study demonstrates that aligned PCL/gelatin mats containing epsilon-PL have broad antimicrobial activity and excellent biocompatibility, making them promising for potential use in wound dressings.
ADVANCED FIBER MATERIALS
(2023)
Article
Chemistry, Physical
Hasaeam Cho, Jimin Lee, Hanjoon Nho, Keunmin Lee, Bopil Gim, Juncheol Lee, Jaehee Lee, Kai K. Ewert, Youli Li, Stuart C. Feinstein, Cyrus R. Safinya, Kyeong Sik Jin, Myung Chul Choi
Summary: Aggregated and hyperphosphorylated Tau is a pathological hallmark of Alzheimer's disease. This study presents experimental results on the ionic strength dependence of the radius of gyration (Rg) of human Tau 4RS and 4RL isoforms for the first time. The findings provide insights into the ion-responsive nature of intrinsically disordered and polyampholytic Tau and its implications for the study of Tau-Tau and Tau-tubulin intermolecular structure in ionic environments.
EUROPEAN PHYSICAL JOURNAL E
(2023)
Article
Engineering, Chemical
Shao-Wei Tsai, Larissa Schwinghammer, Chien-Hsien Lee, Cheng-Fang Lin, Chia-Hung Hou
Summary: This study demonstrated the enhanced simultaneous nitrification and denitrification (SND) in aerobic immobilized biomass using cellulose triacetate as bioplates. The immobilized bioplate reactor (IBPR) achieved higher SND efficiency under intermittent aeration (IA) mode compared to continuous aeration (CA) mode. Microbial community analysis showed that nitrogen-related functional bacteria were more abundant in the bioplates, with colocalization of nitrifiers and denitrifiers observed.
Article
Chemistry, Physical
Maria I. Echeverria I. Molina, Chi-An Chen, Jeniree Martinez, Perry Tran, Kyriakos Komvopoulos
Summary: Fully functional skin regeneration is still difficult after decades of research. Scaffold engineering, a leading field in skin substitutes, can optimize the biomolecular exchange to promote the formation of high-quality tissue. This study designed and evaluated a new class of three-dimensional fibrous scaffolds that induce keratinocyte differentiation through calcium leaching, demonstrating their potential for tissue engineering.
Article
Polymer Science
Xiang Gao, Meiling Wen, Yang Liu, Tian Hou, Bin Niu, Meiwen An
Summary: As a potential material for tissue regeneration, electrospun fibers can mimic the structure of natural extracellular matrix and promote cell growth and tissue integration. In this study, PLCL/CMCS composite fibers were prepared and the effect of CMCS content on fiber properties and cytocompatibility was investigated.