期刊
BIOINFORMATICS
卷 30, 期 10, 页码 1471-1472出版社
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btu036
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资金
- National Health and Medical Research Council [1051757, 1010620, 1063559]
- Cancer Institute of New South Wales [11/REG/1-10]
- National Breast Cancer Foundation
- Commonwealth Scientific and Industrial Research Organizations Transformational Capability Platform
- Science and Industry Endowment Fund
- Information Management and Technology Services
- National Health and Medical Research Council of Australia [1063559] Funding Source: NHMRC
The initial steps in the analysis of next-generation sequencing data can be automated by way of software 'pipelines'. However, individual components depreciate rapidly because of the evolving technology and analysis methods, often rendering entire versions of production informatics pipelines obsolete. Constructing pipelines from Linux bash commands enables the use of hot swappable modular components as opposed to the more rigid program call wrapping by higher level languages, as implemented in comparable published pipelining systems. Here we present Next Generation Sequencing ANalysis for Enterprises (NGSANE), a Linux-based, high-performance-computing-enabled framework that minimizes overhead for set up and processing of new projects, yet maintains full flexibility of custom scripting when processing raw sequence data.
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