Article
Chemistry, Medicinal
Prisca Lagardere, Romain Mustiere, Nadia Amanzougaghene, Sebastien Hutter, Marion Casanova, Jean-Francois Franetich, Shahin Tajeri, Aurelie Malzert-Freon, Sophie Corvaisier, Nadine Azas, Patrice Vanelle, Pierre Verhaeghe, Nicolas Primas, Dominique Mazier, Nicolas Masurier, Vincent Lisowski
Summary: The need for new compounds effective against multiple stages of Plasmodium falciparum development is urgent due to the increasing number of strains resistant to current treatments. In this study, 25 new 4-amino-substituted analogues based on Gamhepathiopine were synthesized and evaluated on both erythrocytic and hepatic stages of the parasite. A promising compound, N2-(tert-butyl)-N [4]-(3-(dimethylamino)propyl)-6-(p-tolyl)thieno[3,2-d]pyrimidine-2,4-diamine, showed improved physicochemical properties, intestinal permeability, and microsomal stability compared to Gamhepathiopine, while maintaining good anti-plasmodial activity on the erythrocytic and hepatic stages of Plasmodium falciparum and P. berghei.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Microbiology
Brittany N. Araj, Bruce Swihart, Robert Morrison, Patricia Gonzales Hurtado, Andrew Teo, Almahamoudou Mahamar, Oumar Attaher, Bacary S. Diarra, Santara Gaoussou, Djibrilla Issiaka, Alassane Dicko, Patrick E. Duffy, Michal Fried
Summary: The study utilized proteomic analyses of PfEMP1 from clinical parasite isolates collected from Malian children to identify targets of immunity. Peptide-specific antibody responses in children were examined, and it was found that high antibody levels to specific PfEMP1 domains correlated with decreased parasite burden in future infections. These findings suggest that certain PfEMP1 domains play a role in protective immunity against malaria.
Article
Multidisciplinary Sciences
Katharina Preissinger, Miklos Kellermayer, Beata G. Vertessy, Istvan Kezsmarki, Janos Torok
Summary: Malaria, a global burden with millions of annual cases, requires improvement in diagnostic techniques for its elimination. This study introduces a novel neural network-based scheme capable of high-speed and accurate classification of malaria infected red blood cells. The smart reduction of data dimension significantly improves the performance and applicability of the method.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Pei-Fen Zhang, Zi-Yi Wu, Wen-Bin Zhang, Yong-Qiao He, Kexin Chen, Tong-Min Wang, Haixin Li, Hong Zheng, Dan-Hua Li, Da-Wei Yang, Ting Zhou, Chang-Mi Deng, Ying Liao, Wen-Qiong Xue, Lian-Jing Cao, Xi-Zhao Li, Jiang-Bo Zhang, Si-Qi Dong, Fang Wang, Mei-Qi Zheng, Wen-Li Zhang, Jianbing Mu, Wei-Hua Jia
Summary: Researchers have discovered a potential biomarker, oncofetal chondroitin sulfate (ofCS), for pan-cancer detection. They developed a sensitive plasma detection method to quantitate the levels of ofCS and found that cancer patients have significantly higher levels of ofCS. The study suggests that developing multi-cancer early detection tests based on cfDNA or protein markers holds great promise.
NATURE COMMUNICATIONS
(2023)
Article
Parasitology
Minghao Zheng, Mengyao Zhang, Hao Li, Shipo Wu, Yuee Zhao, Jinlong Zhang, Yunyue Zhou, Mohamed Boie Jalloh, Kun Zhang, Lina Chen, Zhiqiang Mi, Yong Cui, Lihua Hou
Summary: Rapid and convenient detection of Plasmodium falciparum, the deadliest malaria parasite in Africa, is crucial for malaria eradication efforts in medical resource-limited settings. A CRISPR-based diagnostic method that can detect P. falciparum without nucleic acid extraction was developed and showed high sensitivity and consistency with qPCR test. It can also detect artemisinin-resistant strains and other laboratory-cultured strains. This method has great potential for clinical testing and large-scale community screening in Africa and other regions.
Article
Immunology
Kazutoyo Miura, Eizo Takashima, Thao P. Pham, Bingbing Deng, Luwen Zhou, Wei-Chiao Huang, Ababacar Diouf, Yonas T. Gebremicale, Mayumi Tachibana, Tomoko Ishino, C. Richter King, Jonathan F. Lovell, Carole A. Long, Takafumi Tsuboi
Summary: This study identifies functional epitopes within the malaria transmission-blocking vaccine Pfs230, using antibody depletion and enrichment techniques. Multiple functional epitopes were found and the feasibility of using synthetic peptide antigens for a malaria TBV was established.
Article
Parasitology
Muhydeen Abiodun Abdulraheem, Medard Ernest, Ifeoma Ugwuanyi, Hussein M. Abkallo, Saori Nishikawa, Mofeyisade Adeleke, Adebola E. Orimadegun, Richard Culleton
Summary: Asymptomatic malaria is prevalent among adolescents in Nigeria, with a high rate of co-infections of Plasmodium malariae and Plasmodium ovale. Symptomatic infections are predominantly caused by single infections of Plasmodium falciparum.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2022)
Article
Immunology
Maartje R. Inklaar, Roos M. de Jong, Ezra T. Bekkering, Hikaru Nagaoka, Felix L. Fennemann, Karina Teelen, Marga van de Vegte-bolmer, Geert-Jan van Gemert, Rianne Stoter, C. Richter King, Nicholas I. Proellochs, Teun Bousema, Eizo Takashima, Takafumi Tsuboi, Matthijs M. Jore
Summary: This study identifies a new target for malaria transmission-blocking vaccines, Domain 7 of Pfs230, which can induce antibodies to block parasite development in mosquitoes. The study also discovers a new monoclonal antibody, mAb 18F25.2a, that significantly reduces mosquito infection with Plasmodium.
Article
Cell Biology
James A. Watson, Sophie Uyoga, Perpetual Wanjiku, Johnstone Makale, Gideon M. Nyutu, Neema Mturi, Elizabeth C. George, Charles J. Woodrow, Nicholas P. J. Day, Philip Bejon, Robert O. Opoka, Arjen M. Dondorp, Chandy C. John, Kathryn Maitland, Thomas N. Williams, Nicholas J. White
Summary: Severe malaria caused by Plasmodium falciparum is difficult to accurately diagnose in children in high-transmission settings. A combination of platelet count and plasma PfHRP2 concentration can be used to identify severe falciparum malaria in severely ill patients. About one-third of children enrolled in clinical studies of severe malaria in high-transmission settings in Africa had other causes of their severe illness.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Gladys J. Keitany, Bethany J. Jenkins, Harold T. Obiakor, Shaji Daniel, Atis Muehlenbachs, Jean-Philippe Semblat, Benoit Gamain, Justin Y. A. Doritchamou, Sanjay A. Desai, Nicholas J. MacDonald, David L. Narum, Robert Morrison, Tracy Saveria, Marissa Vignali, Andrew Oleinikov, Michal Fried, Patrick E. Duffy
Summary: This study characterizes an invariant protein associated with placental malaria (PM), PfCSA-L, which binds both VAR2CSA and placental CSA with high affinity. Unlike VAR2CSA, PfCSA-L is peripherally associated with the outer surface of knobs through protein-protein interactions with VAR2CSA. The findings suggest that sequestration of infected red blood cells (iRBCs) involves complexes of invariant and variant surface proteins, allowing parasites to maintain diversity and function on the iRBC surface.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
DeAnna J. Friedman-Klabanoff, Mark A. Travassos, Olukemi O. Ifeonu, Sonia Agrawal, Amed Ouattara, Andrew Pike, Jason A. Bailey, Matthew Adams, Drissa Coulibaly, Kirsten E. Lyke, Matthew B. Laurens, Shannon Takala-Harrison, Bourema Kouriba, Abdoulaye K. Kone, Ogobara K. Doumbo, Jigar J. Patel, Mahamadou A. Thera, Philip L. Felgner, John C. Tan, Christopher Plowe, Andrea A. Berry
Summary: In a study conducted in Mali, it was found that adults had stronger antibody responses to circumsporozoite protein (CSP) compared to children, and responded to more variants near the central repeat region. Children acquired short-lived immunity to an epitope proximal to the central repeat region, but not to the central repeat region itself. This approach has the potential to differentiate immunodominant from protective epitope-specific responses when combined with longitudinal infection data.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Chemistry, Medicinal
Theresa Hermann, Patrick Hochegger, Johanna Dolensky, Werner Seebacher, Robert Saf, Marcel Kaiser, Pascal Maser, Robert Weis
Summary: The N-acylated furazan-3-amine derivative showed promising antiplasmodial activity against different strains of Plasmodium falciparum, with benzamides being the most active. The substitution pattern of the phenyl ring affected the activity, while the N-(4-(3,4-diethoxyphenyl)-1,2,5-oxadiazol-3-yl)-3-(trifluoromethyl)benzamide exhibited good physicochemical properties and high antiplasmodial activity against both chloroquine-sensitive and multiresistant strains.
Article
Microbiology
Mariko Kanai, Tomas Yeo, Victor Asua, Philip J. Rosenthal, David A. Fidock, Sachel Mok
Summary: The study presents a SNP-based genotyping method that enables the rapid characterization of drug resistance in Plasmodium falciparum parasites. The method utilizes both multiplexed fragment analysis and gel electrophoresis to distinguish different strains. The results demonstrate the potential applications of the method in drug resistance screening, verifying SNP-editing, identifying novel genetic cross progeny, and inferring geographical origins.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Luis Izquierdo
Summary: Like other microorganisms, pathogenic protozoan parasites rely on glycoconjugates and glycan binding proteins to survive and interact with their hosts. Understanding how glycobiology contributes to their survival and virulence can reveal new aspects of their biology and lead to the development of new strategies against them.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Immunology
Janavi S. Rambhatla, Gerry Q. Tonkin-Hill, Eizo Takashima, Takafumi Tsuboi, Rintis Noviyanti, Leily Trianty, Boni F. Sebayang, Daniel A. Lampah, Jutta Marfurt, Ric N. Price, Nicholas M. Anstey, Anthony T. Papenfuss, Timon Damelang, Amy W. Chung, Michael F. Duffy, Stephen J. Rogerson
Summary: The study found that levels of antibodies to PfEMP1 domains are associated with the severity of malaria. Three domains of PfEMP1 were highly discriminatory between severe and uncomplicated malaria. Lack of antibodies to locally expressed PfEMP1 types may partially explain the severity of malaria in Papuan adults.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Zhi Wang, Alexandra S. Tauzin, Elisabeth Laville, Pietro Tedesco, Fabien Letisse, Nicolas Terrapon, Pascale Lepercq, Myriam Mercade, Gabrielle Potocki-Veronese
Article
Biology
Junhua Li, Huanzi Zhong, Yuliaxis Ramayo-Caldas, Nicolas Terrapon, Vincent Lombard, Gabrielle Potocki-Veronese, Jordi Estelle, Milka Popova, Ziyi Yang, Hui Zhang, Fang Li, Shanmei Tang, Fangming Yang, Weineng Chen, Bing Chen, Jiyang Li, Jing Guo, Cecile Martin, Emmanuelle Maguin, Xun Xu, Huanming Yang, Jian Wang, Lise Madsen, Karsten Kristiansen, Bernard Henrissat, Stanislav D. Ehrlich, Diego P. Morgavi
Article
Genetics & Heredity
Ao Li, Elisabeth Laville, Laurence Tarquis, Vincent Lombard, David Ropartz, Nicolas Terrapon, Bernard Henrissat, David Guieysse, Jeremy Esque, Julien Durand, Diego P. Morgavi, Gabrielle Potocki-Veronese
MICROBIAL GENOMICS
(2020)
Article
Microbiology
Leidy Lagos, Sabina Leanti La Rosa, Magnus O. Arntzen, Ragnhild Anestad, Nicolas Terrapon, John Christian Gaby, Bjorge Westereng
Correction
Multidisciplinary Sciences
Didier Ndeh, Arnaud Basle, Henrik Strahl, Edwin A. Yates, Urszula L. McClurgg, Bernard Henrissat, Nicolas Terrapon, Alan Cartmell
NATURE COMMUNICATIONS
(2020)
Article
Microbiology
Alexandra S. Tauzin, Mariana Rangel Pereira, Liisa D. Van Vliet, Pierre-Yves Colin, Elisabeth Laville, Jeremy Esque, Sandrine Laguerre, Bernard Henrissat, Nicolas Terrapon, Vincent Lombard, Marion Leclerc, Joel Dore, Florian Hollfelder, Gabrielle Potocki-Veronese
Article
Microbiology
Michael L. Patnode, Janaki L. Guruge, Juan J. Castillo, Garret A. Couture, Vincent Lombard, Nicolas Terrapon, Bernard Henrissat, Carlito B. Lebrilla, Jeffrey Gordon
Summary: By creating a panel of artificial food particles, researchers unveiled the binding phenotypes of gut microbiota with dietary or host glycans, confirming the specific binding of different bacterial strains to various carbohydrates and metabolic differences. These adhesion phenotypes were shown to be maintained in more complex communities through mixed in vitro cultures.
CELL HOST & MICROBE
(2021)
Article
Genetics & Heredity
Shicheng Chen, Ting Yu, Nicolas Terrapon, Bernard Henrissat, Edward D. Walker
Summary: Asaia bacteria play a key role in the microbiome of many mosquito species, potentially delivering antimalaria or antivirus effectors through paratransgenesis. Strain W12 has the largest genome size among Asaia species, with more glycoside hydrolase-encoding genes and predicted regulatory protein components, indicating its potential for plant biomass degradation and adaptation to environmental changes in the mosquito gut. Additionally, Asaia sp. W12 carries genes involved in the production of 2,3-butandiol and inositol, possibly contributing to biofilm formation and stress tolerance.
Article
Biotechnology & Applied Microbiology
Lisanne Hameleers, Leena Penttinen, Martina Ikonen, Lea Jaillot, Regis Faure, Nicolas Terrapon, Peter J. Deuss, Nina Hakulinen, Emma R. Master, Edita Jurak
Summary: The study identified a novel bifunctional carbohydrate esterase (CE), BD-FAE, encoded within PULs of metagenomes from beaver droppings and moose rumen enriched on poplar hydrolysate. BD-FAE displayed feruloyl esterase (FAE) and acetyl esterase activity on synthetic and natural substrates, making it a new candidate for biomass processing.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Elodie Drula, Marie-Line Garron, Suzan Dogan, Vincent Lombard, Bernard Henrissat, Nicolas Terrapon
Summary: The CAZy database, freely available for browsing and download, is deeply rooted in human curation and plays a crucial role in maintaining and updating family classification, classifying new sequences, and presenting functional information. Over the past 8 years, there has been an increase in novel families and extensive annotations conducted, highlighting the significant amount of work involved in analyzing and reporting biochemical data from the literature.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Lucelia Cabral, Gabriela F. Persinoti, Douglas A. A. Paixao, Marcele P. Martins, Mariana A. B. Morais, Mariana Chinaglia, Mariane N. Domingues, Mauricio L. Sforca, Renan A. S. Pirolla, Wesley C. Generoso, Clelton A. Santos, Lucas F. Maciel, Nicolas Terrapon, Vincent Lombard, Bernard Henrissat, Mario T. Murakami
Summary: In this study, Cabral et al. analyzed the gut microbiome of capybara, the largest living rodent, and identified enzymatic mechanisms for the breakdown of lignocellulosic biomass. They also discovered two families of carbohydrate-active enzymes. The findings shed light on the microbial community composition and metabolic pathways involved in the conversion of dietary fibers into short-chain fatty acids, and provide insights into the untapped reservoir of enzymatic mechanisms in the capybara gut microbiota.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ketty C. Tamburrini, Nicolas Terrapon, Vincent Lombard, Bastien Bissaro, Sonia Longhi, Jean-Guy Berrin
Summary: Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze the oxidative cleavage of polysaccharides. A study analyzing 27,060 LPMO sequences found that 60% of them have a predicted intrinsically disordered C-terminal extension, indicating a common polyampholytic nature and potential for post-translational modifications. This widespread occurrence of disordered C-terminal regions in LPMOs suggests a possible functional role and opens up new prospects in this field.
Article
Multidisciplinary Sciences
Marie Couturier, Melanie Touvrey-Loiodice, Nicolas Terrapon, Elodie Drula, Laurine Buon, Christine Chirat, Bernard Henrissat, William Helbert
Summary: This study focuses on the functional characterization of GH113 family enzymes, revealing their specificity towards mannans with variations in product profiles and modes of action. Four clades of the family were assigned mannanase and mannosidase activities, increasing the number of characterized GH113 members by 200% and expanding the toolbox for fine-tuning of mannooligosaccharides.
Article
Biochemical Research Methods
Bastian Volker Helmut Hornung, Nicolas Terrapon
Summary: To improve protein classification and annotation, researchers have developed a new method to identify subfamilies using sequence similarity networks. They found that the connectedness within the network, as measured by closeness centrality, closely matched the classification decisions of experts. This method allows for faster creation of new protein subfamilies.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Leszek Michalak, John Christian Gaby, Leidy Lagos, Sabina Leanti La Rosa, Torgeir R. Hvidsten, Catherine Tetard-Jones, William G. T. Willats, Nicolas Terrapon, Vincent Lombard, Bernard Henrissat, Johannes Droge, Magnus Overlie Arntzen, Live Heldal Hagen, Margareth Overland, Phillip B. Pope, Bjorge Westereng
NATURE COMMUNICATIONS
(2020)