Article
Chemistry, Analytical
Gaihua Cao, Yue Qiu, Keyi Long, Yifan Xiong, MeimeiShi, JunYang, Yingguo Li, Fuping Nie, Danqun Huo, Changjun Hou
Summary: In this study, a fluorescence biosensor based on carbon nanodots and loop-mediated isothermal amplification was established for highly sensitive detection of African swine fever virus (ASFV). The biosensor showed rapid, sensitive, low-cost, and simple detection of ASFV, with high accuracy in actual sample assays.
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Horticulture
Chengzhong Lan, Lin Gan, Yuli Dai, Xiaofei Liu, Xiujuan Yang
Summary: Round leaf spot disease caused by M. acerina is a highly destructive disease in Sanqi (Panax notoginseng) plantations in China. This study developed a specific and sensitive loop-mediated isothermal amplification (LAMP) assay for detecting M. acerina using primers targeting the internal transcribed spacer (ITS) region. The optimized LAMP assay showed high specificity and sensitivity, successfully detecting M. acerina in both symptomatic and latently infected leaf samples. The LAMP assay has the potential to be an efficient and specific method for diagnosing and managing round leaf spot disease in the field.
Article
Environmental Sciences
Qiqi Xue, Lingwei Sheng, Junjie Guo, Minghui Zou, Qiao Teng, Sijia Xu, Yuanyuan Li, Jinhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was successfully established for the detection of the Dermatophagoides farinae allergen. This method showed higher sensitivity, specificity, and efficiency compared to conventional PCR. It did not cross-react with other arthropod species commonly found in indoor dust. This study provides a potential model for the rapid detection of allergens produced by other house dust mites in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Food Science & Technology
Alfonsina Fiore, Ida Treglia, Gianni Ciccaglioni, Marco Francesco Ortoffi, Antonietta Gattuso
Summary: Changing eating habits and rising demand of food have led to an increase in foodborne diseases, particularly in industrialized countries. Contaminated ready-to-eat food can transmit Listeria monocytogenes, a foodborne pathogen responsible for listeriosis. This study successfully applied a colorimetric Loop-mediated isothermal amplification (LAMP) assay to rapidly detect L. monocytogenes in experimentally contaminated RTE meat samples. The results showed high specificity and sensitivity compared to traditional methods, suggesting that colorimetric LAMP assays can be used as a screening tool for L. monocytogenes in ready-to-eat meat food.
Article
Chemistry, Applied
Li Qiuping, Cheng Yinghao, Xu Wenjie, Cui Xiaowen, Cao Min, Xiong Xiaohui, Wang Libin, Xiong Xiong
Summary: In this study, Loop-mediated isothermal amplification (LAMP) coupled with a self-quenching approach was developed for accurate species identification in fish. The novel assay showed high tolerance to impurities and produced reliable positive results under different DNA extraction methods and sample treatments. Additionally, using DNA with higher purity improved LAMP amplification.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Analytical
Maud Savonnet, Mathilde Aubret, Patricia Laurent, Yoann Roupioz, Myriam Cubizolles, Arnaud Buhot
Summary: This paper presents a theoretical framework to analyze the kinetics of the second stage of LAMP, which can help optimize LAMP performance by reducing the number of experiments needed to find the best parameters.
Article
Chemistry, Analytical
Ane Rivas-Macho, Unai Eletxigerra, Ruth Diez-Ahedo, Angela Barros, Santos Merino, Felipe Goni-de-Cerio, Garbine Olabarria
Summary: An electrochemical sensor based on loop-mediated isothermal amplification (LAMP) technology was developed for the detection of SARS-CoV-2 virus in clinical samples. The sensor eliminates the need for DNA extraction by heating the sample, reducing the time required for each measurement.
Article
Biochemistry & Molecular Biology
Igor P. Oscorbin, Georgiy Yu. Shevelev, Ksenia A. Pronyaeva, Andrey A. Stepanov, Darya V. Shamovskaya, Olga V. Mishukova, Dmitrii V. Pyshnyi, Maksim L. Filipenko
Summary: Loop-mediated isothermal amplification (LAMP) is a stable nucleic acid amplification method that is resistant to DNA amplification inhibitors, allowing for multiplexing and suitable for SARS-CoV-2 viral RNA detection with high accuracy and short analysis time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Wenjuan Wu, Congcong Yin, Aiqin Yue, Jingping Niu, Weijun Du, Dingbin Liu, Jinzhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for visual and rapid detection of the major prevalent SMV strain in China. The assay showed high analytical performance and potential for early detection of SMV-SC7-associated plant diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Plant Sciences
Huiying Sun, Lin Sun, Liuliu Yang, Zehao Wang, Zihao Xia, Xinyu Yang, Zhiwei Jiao, Jie Feng, Yue Liang
Summary: A rapid and sensitive detection method based on LAMP technology was successfully developed in this study for accurate detection of the causal agent of sunflower black stem. This method not only has the characteristics of rapid and sensitive detection, but also can be applied to different types of infected tissues and seed samples, which is of great significance for assessing the prevalence and implementing sustainable management of the disease.
Article
Physics, Multidisciplinary
Sarunrit Kesornkhup, Adisorn Tuantranont, Tanom Lomas, Chakrit Sriprachuabwong, Kamol Wasapinyokul
Summary: The study examined the accuracy of the Swanepoel method in calculating the thickness of spin-coated polymer films, revealing that films with higher solution concentrations or slower spin-coating speeds tend to be thicker. Errors between Swanepoel and actual thickness were mainly influenced by film surface roughness.
ACTA PHYSICA POLONICA A
(2021)
Article
Chemistry, Physical
Nantikan Tammanoon, Anurat Wisitsoraat, Adisorn Tuantranont, Chaikarn Liewhiran
Summary: This study investigates the sensitivity and selectivity of Zn-substituted SnO2 nanoparticles towards formic acid, demonstrating their potential for ultrasensitive and selective detections of HCOOH in practical applications. The results show that a sensing layer with the optimum Zn content of 0.2 wt% provided a remarkably high response towards 1000 ppm HCOOH, indicating a significant improvement in performance compared to undoped samples. Additionally, good selectivity against various volatile organic compounds was achieved, highlighting the promising applications of the flame-made Zn-substituted SnO2 sensors.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Chemistry, Analytical
K. Khamfoo, A. Wisitsoraat, M. Punginsang, A. Tuantranont, C. Liewhiran
Summary: Nb-doped SnO2 nanoparticles were synthesized via flame spray pyrolysis for the first time, with 0.5 wt% Nb content providing the best sensor response to 1 vol% C2H2 at 350 degrees Celsius. The sensor also exhibited low humidity dependence, good long-term stability, and high C2H2 selectivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Suphatsorn Thimoonnee, Kanpitcha Somnet, Pattanun Ngaosri, Sanoe Chairam, Chanpen Karuwan, Wichayaporn Kamsong, Adisorn Tuantranont, Maliwan Amatatongchai
Summary: We report a new ready-to-use sensor for simultaneous determination of paraquat (PQ) and glyphosate (GLY) based on a graphite screen-printed electrode modified with a dual-molecularly imprinted polymer coated on a mesoporous silica-platinum core. The sensor shows good linear calibration curves and high selectivity, making it suitable for simultaneous detection of PQ and GLY concentrations in water samples.
ANALYTICAL METHODS
(2022)
Article
Electrochemistry
Wichayaporn Kamsong, Vitsarut Primpray, Patiya Pasakon, Chakrit Sriprachuabwong, Saithip Pakapongpan, Johannes Philipp Mensing, Anurat Wisitsoraat, Adisorn Tuantranont, Chanpen Karuwan
Summary: A highly sensitive and disposable screen-printed ionic liquid-graphene electrode (SPIL-GE) was developed for electrochemical sensing. The electrode showed improved electrochemical performance compared to other types of electrodes, making it a potential candidate for advanced electrochemical sensing applications.
ELECTROCHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Johannes Philipp Mensing, Tanom Lomas, Adisorn Tuantranont
Summary: In this study, high performance LICs were successfully developed using rGO@Pd and activated carbon materials, demonstrating excellent electrochemical performance and cycle life.
Article
Chemistry, Inorganic & Nuclear
Surangkana Wannapop, Anuwat Khawsaad, Apisit Supanpong, Yupin Janorat, Yaowamarn Chuminjak, Adisorn Tuantranont, Anukorn Phuruangrat, Titipun Thongtem, Somchai Thongtem, Asanee Somdee
Summary: In this study, the role of p-n junction in the photocatalytic degradation of dye-contaminated wastewater was investigated by synthesizing n-type ZnO enhanced with metal oxides like CuO, NiO, and Fe2O3. The performance of CuO/ZnO and NiO/ZnO materials was found to be better compared to pure ZnO, while Fe2O3/ZnO exhibited decreased photocatalytic activity. Ni(5)-ZnO showed the highest performance, degrading dyes by over 99.71% in contaminated water.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Pakapreud Khumwan, Sukanya Pengpanich, Jantana Kampeera, Wichayaporn Kamsong, Chanpen Karuwan, Assawapong Sappat, Prapaporn Srilohasin, Angkana Chaiprasert, Adisorn Tuantranont, Wansika Kiatpathomchai
Summary: We developed a LAMP-based technique that utilizes primers with base mismatches to specifically detect isoniazid resistance in tuberculosis. This technique demonstrates high sensitivity and specificity, and can be performed on a handheld potentiostat device for endpoint detection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Saithip Pakapongpan, Yingyot Poo-arporn, Adisorn Tuantranont, Rungtiva P. Poo-arporn
Summary: In this study, a simple one-pot solvothermal synthesis approach was used to fabricate a magnetic iron oxide embed nitrogen-doped graphene nanohybrid. This nanohybrid was then applied in an electrochemical sensor for the ultrasensitive detection of antibiotic residue contamination in milk samples. The sensor demonstrated excellent performance with high sensitivity, selectivity, and fast detection.
Article
Food Science & Technology
Nattanicha Chaiya, Donraporn Daranarong, Patnarin Worajittiphon, Runglawan Somsunan, Puttinan Meepowpan, Adisorn Tuantranont, Nawasit Rakbamrung, Paul D. D. Topham, Brian J. J. Tighe, Anisa Mahomed, Winita Punyodom
Summary: This study aimed to improve the properties of poly(lactic acid) (PLA) by melt blending with non-toxic poly(ethylene oxide) (PEO), and fabricate a biodegradable humidity sensing substrate using solvent-cast 3D printing. The results showed that the PLA/PEO blend exhibited enhanced flexibility and thermal stability, along with increased hydrophilicity with higher PEO content.
FOOD PACKAGING AND SHELF LIFE
(2022)
Article
Chemistry, Physical
Johannes Philipp Mensing, Tanom Lomas, Adisorn Tuantranont
Summary: Rechargeable magnesium batteries (RMBs) have the potential to contribute to the shift towards renewable and green energy sources, but still face challenges that need to be overcome. Graphene is being extensively studied for its ability to improve the performance of RMBs by enhancing electrochemical characteristics and mechanical stability of battery components.
Article
Polymer Science
Manasanan Namhongsa, Donraporn Daranarong, Robert Molloy, Sukunya Ross, Gareth M. Ross, Adisorn Tuantranont, Dheerawan Boonyawan, Jiraporn Tocharus, Sivanan Sivasinprasasn, Paul D. Topham, Brian J. Tighe, Winita Punyodom
Summary: In this study, two-component scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with N-2-Ar plasma to enhance their surface properties. The plasma treatment significantly improved the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided mechanical strength, while the electrospun fiber component promoted cell attachment and proliferation. The designed scaffolds showed potential for supporting cell regeneration in biomedical applications.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Tanaporn Kongthong, Chatwarin Poochai, Chakrit Sriprachuabwong, Adisorn Tuantranont, Suwat Nanan, Nonglak Meethong, Pasit Pakawatpanurut, Taweechai Amornsakchai, Jedsada Sodtipinta
Summary: This study investigates a composite material consisting of porous, N-doped activated carbon derived from pineapple leaf fiber (PALF-NAC) and alpha-MnO2 nanofibers as an electrode material for high-performance supercapacitors (SCs). The PALF-NAC and alpha-MnO2 /PALF-NAC possess mesoporous structures with high specific surface areas. The alpha-MnO2/PALF-NAC electrode exhibits high specific capacitance and excellent cycling stability, making it a promising candidate for stable, cost-effective, and high-performance supercapacitors.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2022)
Article
Chemistry, Analytical
Kanpitcha Somnet, Suphatsorn Thimoonnee, Chanpen Karuwan, Wichayaporn Kamsong, Adisorn Tuantranont, Maliwan Amatatongchai
Summary: A novel electrochemical sensor based on a screen-printed graphene paste electrode modified with platinum nanoparticles and coated with a molecularly imprinted polymer was proposed for sensitive and cost-effective detection of paraquat herbicide. The sensor showed high sensitivity, selectivity, and a lower detection limit for quantification of paraquat residue on vegetables and fruits. The ready-to-use MIP sensor offers an alternative approach for food safety applications.
Article
Chemistry, Multidisciplinary
Korkrit Songmueang, Dongdong Zhang, Jin Cao, Xinyu Zhang, Soorathep Kheawhom, Chakrit Sriprachuabwong, Adisorn Tuantranont, Panyawat Wangyao, Jiaqian Qin
Summary: This research developed a flower-like W/WO3 hybrid as a cathode for ZIBs, showing exceptional rate performance and cycling performance. Additionally, the reversible Zn2+ insertion/extraction mechanism in W/WO3 was demonstrated for the first time, along with the impressive flexibility and capabilities of the soft-packaged batteries.
CHEMICAL COMMUNICATIONS
(2021)