Article
Agriculture, Multidisciplinary
Zhi Jin, Weili Cui, Jianfeng Ma, Qian Chen, Yuejin Fu
Summary: Confocal Raman microspectroscopy was used to observe the dissolution of hydroxycinnamates (HCMs) incorporated into lignin-carbohydrate complexes (LCCs) in energy crops Miscanthus sinensis cv. during NaOH treatment. The results showed that mild NaOH treatment led to a higher proportion of HCM depolymerization in highly lignified middle lamella areas compared to carbohydrate-abundant secondary walls. Raman imaging revealed that lignin depolymerization was more pronounced in the sclerenchyma fiber and parenchyma secondary wall with increasing treatment time, while the depolymerization of HCMs was closely related to lignin depolymerization. A better understanding of these processes is important for efficient breaking of LCC bonds in herbaceous biomass.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
JeongEun Kang, JiSun Park, Jung-Kil Seo, WonHyung Choi, SooCheol Choi, Ju-Hyoung Kim, In-Ah Lee
Summary: The study demonstrated that Ulva ohnoi oil has anti-inflammatory properties, reducing the production of nitric oxide and infiltration of inflammatory cells during inflammation, showing potential as an anti-inflammatory agent.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Tianyu Gao, Alexander J. Boys, Crystal Zhao, Kiara Chan, Lara A. Estroff, Lawrence J. Bonassar
Summary: This study utilized confocal Raman microspectroscopy to investigate the degradation of articular cartilage, specifically focusing on spatially mapping the loss of glycosaminoglycans (GAGs), providing a technique with high spatial resolution to measure cartilage degradation without tissue sections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Xiao Wei, Fang Li, Anand Babu Perumal, Alireza Sanaeifar, Mahamed Lamine Guindo, Yongqiang Shi, Yong He, Fei Liu
Summary: This study investigated the feasibility of using confocal Raman microspectroscopy (CRM) combined with spectral screening algorithms for quantitative detection of starch in rice. The experimental results demonstrated that after proper pre-processing and spectral screening algorithm, CRM could swiftly and accurately detect the starch content in rice.
FOOD HYDROCOLLOIDS
(2023)
Article
Construction & Building Technology
Jiseul Park, Myungjun Jung, Yang-woo Lee, Hee-Young Hwang, Sung-gul Hong, Juhyuk Moon
Summary: Dispersion of carbon nanotubes (CNTs) is crucial for the performance of nanocomposites. However, the evaluation of CNT dispersion is limited. This study visualized the macroscopic distribution of CNTs in hardened cement matrix using confocal Raman microspectroscopy and optical microscope. The dispersion efficiency and heterogeneity were quantified to assess the dispersion quality. The experimental results highlight the importance of well-dispersed CNT suspension and suggest evaluating ultrasonication with superplasticizers for dispersion in cement composites.
CEMENT AND CONCRETE RESEARCH
(2023)
Article
Plant Sciences
Alireza Sanaeifar, Dapeng Ye, Xiaoli Li, Liubin Luo, Yu Tang, Yong He
Summary: The present study characterized the temporal and spatial variation of biopolymers in cells infected by the tea leaf blight using confocal Raman microspectroscopy. The study found that lignin and phenolic compounds decreased in the vascular bundle as the infection progressed, while cellulose and polysaccharides increased in all parts. The distribution of biopolymers varied with the severity of infection, and a substance was produced within the vascular bundles to prevent the further development of pathogens.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Jin Zhang, Pengya Gao, Yuan Wu, Xiaomei Yan, Changyun Ye, Weili Liang, Meiying Yan, Xuefang Xu, Hong Jiang
Summary: This study demonstrates that confocal Raman microspectroscopy combined with chemometrics can serve as a rapid, reliable, and efficient method for the detection and identification of foodborne pathogenic bacteria without any laborious pre-treatments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Johannes Schleusener, Andrew Salazar, Joerg von Hagen, Juergen Lademann, Maxim E. Darvin
Summary: This study investigated the effects of a topically applied formulation containing components of natural moisturizing factor (NMF) on barrier-related parameters of the stratum corneum (SC). It was found that the formulation disrupted the equilibrium of water, NMF, and lipids in the SC, but adverse effects could be mitigated by a simplified NMF supplementation. Furthermore, the supplement unexpectedly prevented intercellular lipids (ICL) disorganization at selected depths, and limited lateral disorganization of ICL to the upper 20% SC depth in the long term. Further research is needed to understand the interaction of these factors in the SC for the development of topical treatments for skin barrier-related diseases.
Article
Forestry
Bing Wang, Mei Luo, Yadi Liu, Xiaorui Guo, Xiatong Liu, Chong Zhang, Zhijing Zhao, Di Liu, Hui Li, Hai Lu
Summary: Confocal Raman microspectroscopy (CRM) is an important tool for analyzing the compositional distribution of cell walls in situ. The study demonstrated that CRM is useful for rapidly analyzing the spatial variation of lignin content in the xylem of woody plants.
JOURNAL OF FORESTRY RESEARCH
(2021)
Article
Spectroscopy
Jin Zhang, Hong Jiang, Pengya Gao, Yuan Wu, Hui Sun, Ying Huang, Xuefang Xu
Summary: The study demonstrates that confocal Raman microspectroscopy combined with chemometrics can be utilized as a fast, reliable, and nondestructive method for detection and identification of Clostridium botulinum at both species and serotypes level without the need for laborious pre-treatments.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Agricultural Engineering
Alireza Sanaeifar, Xiaoli Li, Yong He, Zhenxiong Huang, Zhihao Zhan
Summary: This study focused on the quantitative detection of pesticide residue in tea using electronic nose (e-nose) and confocal Raman microspectroscopy (CRM), and found that combining the data from both technologies provided better prediction performance for pesticide residues in tea. The best prediction performance was achieved with 32 effective variables selected from the fusion dataset by the artificial neural network (ANN) model.
BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemical Research Methods
Ali Jaafar, Roman Holomb, Anton Y. Sdobnov, Zsombor Ocskay, Zoltan Jakus, Valery V. Tuchin, Miklos Veres
Summary: The study investigated the effect of tissue optical clearing (TOC) on ex vivo porcine dura mater using confocal Raman microspectroscopy (CRM) to increase probing depth and observe in-depth structure. It found that the TOC process involves three main steps, including tissue dehydration, glycerol penetration, and collagen dissociation, with glycerol significantly affecting collagen hydration. This study is the first to introduce the TOC technique in assisting CRM of ex vivo dura mater in-depth probing.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Polymer Science
Raffael Rathner, Claudia Leimhofer, Wolfgang Roland, Alexander Hammer, Bernhard Loew-Baselli, Georg Steinbichler, Sabine Hild
Summary: Co-extrusion is a commonly used method for producing polymer multilayer products. The adhesion between layers is crucial for the overall performance of the structure. This study investigated the influence of different processing conditions on layer adhesion and found that adjusting the shear load can significantly improve the adhesion. These findings open up new opportunities and potentials for multi-layer products.
Article
Optics
I. A. Balakhnina, N. N. Brandt, A. E. Dedova, A. A. Zagladin, A. Yu Chikishev
Summary: This study proposes the use of nanosecond laser pulses to obtain microsamples of paint layers and subsequently identify the substances using Raman microspectroscopy. It is demonstrated that the ablation of the paint layer on primed canvas is a two-threshold process. The proposed technique can be employed for the study of paintings.
Article
Chemistry, Analytical
Sergey Kudryashov, Elena Rimskaya, Evgeny Kuzmin, Galina Kriulina, Victoria Pryakhina, Andrey Muratov, Roman Khmelnitskii, Evgeny Greshnyakov, Pavel Danilov, Vladimir Shur
Summary: Natural diamonds were investigated using advanced chemical and structural analytical tools to comprehensively characterize their optically blind and optically active nitrogen impurity centers. The study established compositional relationships between X-ray photoelectron spectroscopy and Fourier-transform infrared vibrational spectroscopy signals of nitrogen impurity defects, and correlated depth-dependent spatial distributions of photoluminescence-active nitrogen defects with the carbon lattice signals. The study also revealed connections between local structural defects and stress-sensitive impurity distributions in bulk diamonds. These findings demonstrate the instrumental opportunities for in situ studies of diamond micro-features.
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Marek Grosicki, Jakub Dybas, Ewelina Matuszyk, Marko Rodewald, Tobias Meyer-Zedler, Michael Schmitt, Juergen Popp, Kamilla Malek, Malgorzata Baranska
Summary: The study compared the response to inflammation between isolated human eosinophils and EoL-1 cells in terms of lipid bodies (LBs) using fluorescence, Raman, and coherent anti-Stokes Raman scattering (CARS) microscopy. Differences were observed in the number and chemical composition of LBs, indicating varying manifestations of inflammatory processes depending on the cell type and stimulation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Maximilian Sender, Fabian L. Huber, Maximilian C. G. Moersch, Daniel Kowalczyk, Julian Hniopek, Sarah Klingler, Michael Schmitt, Simon Kaufhold, Kevin Siewerth, Juergen Popp, Boris Mizaikoff, Dirk Ziegenbalg, Sven Rau
Summary: This work elaborates on the effect of dynamic irradiation on light-driven molecular water oxidation and emphasizes the importance of overall reaction engineering in overcoming limiting factors. By controlling the local availability of photons, the overall catalytic efficiency can be significantly enhanced.
Article
Polymer Science
Josefine Meurer, Thomas Baetz, Julian Hniopek, Milena Jaeger, Stefan Zechel, Michael Schmitt, Juergen Popp, Martin D. Hager, Ulrich S. Schubert
Summary: This study synthesizes and characterizes metallopolymer networks with a triple shape-memory ability. The networks exhibit excellent dual and triple shape-memory abilities and have been analyzed using various techniques.
Article
Environmental Sciences
Nils Meyer, Aljoscha Rydzyk, Georg Pohnert
Summary: This study reveals that diatoms are capable of taking up organic resources from lysates of competing algae, and these resources are partially maintained or undergo metabolic transformation within the cells. This suggests that diatoms have the potential to compete with heterotrophs and may have a significant impact on organic material fluxes in the oceans.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Runqing Yang, Dong Wei, Georg Pohnert
Summary: This study reveals that a combination of tryptone and urea as organic nitrogen sources can enhance fucoxanthin production in mixotrophic cultivation of Phaeodactylum tricornutum. Among the amino acids present in the medium, arginine has the highest nitrogen utilization rate and promotes cell growth the most. The optimal combination of arginine and urea as nitrogen sources leads to the highest biomass concentration, fucoxanthin content, and productivity. This study provides new insights into the promotion of fucoxanthin biosynthesis and suggests a promising strategy for efficient enhancement of fucoxanthin production.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Vera Nikitashina, Daniel Stettin, Georg Pohnert
Summary: Microalgae respond to salinity stress with common adaptations in the metabolome, such as up-regulation of amino acids and production of saccharides and inositols. However, there are also species-specific dysregulations of metabolites. Several metabolites not previously connected to osmotic stress reactions were identified. This study expands our knowledge about osmoadaptation and highlights the need for further functional characterization of metabolites and pathways in algal stress physiology.
Article
Chemistry, Multidisciplinary
Parijat Barman, Abhik Chakraborty, Denis A. Akimov, Ankit Kumar Singh, Tobias Meyer-Zedler, Xiaofei Wu, Carsten Ronning, Michael Schmitt, Juergen Popp, Jer-Shing Huang
Summary: Plasmonic gratings with spatially resolved broadband momentum were used to investigate plasmonic enhancement effects in two nonlinear optical processes, TPPL and SHG. The experimental spatial distributions of nonlinear signals agreed well with analytical predictions, highlighting the antenna function of the grating in plasmonic nonlinear signal generation.
Article
Chemistry, Physical
Thomas Gottschall, Tobias Meyer-Zedler, Matthias Eibl, Tom Pfeiffer, Hubertus Hakert, Michael Schmitt, Robert Huber, Andreas Tunnermann, Jens Limpert, Juergen Popp
Summary: Coherent Raman scattering microscopy utilizing bioorthogonal tagging approaches allows for targeted monitoring of small molecules in biological samples. To fully exploit this technique, a fast tunable laser is needed. The authors present a laser concept without moving parts for simultaneous acquisition of coherent anti-Stokes Raman scattering images at multiple Raman resonances.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Biochemical Research Methods
Rola Houhou, Elsie Quansah, Tobias Meyer-zedler, Michael Schmitt, Franziska Hoffmann, Orlando Guntinas-lichius, Juergen Popp, Thomas Bocklitz
Summary: Biophotonic multimodal imaging techniques are used to study biological samples, but the measurement time increases significantly for high-resolution images. Mathematical methods and artificial intelligence (AI) based approaches were compared in this research to shorten the acquisition time and improve data quality. The AI methods included transfer learning using the pre-trained network DnCNN and training networks using augmented images. Deep learning techniques showed potential in improving image quality and reducing acquisition time. The proposed network had a simpler architecture compared to similar-performing but highly parametrized networks.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Engineering, Biomedical
Justyna Anna Niestrawska, Marko Rodewald, Constanze Schultz, Elsie Quansah, Tobias Meyer-Zedler, Michael Schmitt, Juergen Popp, Igor Tomasec, Benjamin Ondruschka, Niels Hammer
Summary: This study examines the link between location-dependent mechanical anisotropy and principal collagen fiber orientation in the human dura mater. The quantitative observation of collagen fiber orientation and dispersion confirms that collagen is highly aligned in the dura mater and that both fiber orientation and dispersion differ depending on the location investigated. These findings provide insight into the previously observed isotropic mechanical behavior and have implications for the mechanical load response and regional functions of the dura mater.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Franziska A. Klapper, Christine Kiel, Peter Bellstedt, Wim Vyverman, Georg Pohnert
Summary: Diatoms, abundant unicellular microalgae, play a crucial role in global photosynthetic CO2 fixation. However, their sexual reproduction remains poorly understood. In this study, the structure of a sex-inducing pheromone in the diatom Seminavis robusta was elucidated, revealing its mechanism of action and providing important insights into the reproductive process of diatoms.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Edoardo Farnesi, Silvia Rinaldi, Chen Liu, Jonas Ballmaier, Orlando Guntinas-Lichius, Michael Schmitt, Dana Cialla-May, Juergen Popp
Summary: Saliva, as a body fluid, plays an important role in point-of-care medicine for real-time health monitoring and personalized preventative therapy. The combination of SERS and MD simulations can verify the traceability of specific biomarkers in saliva, opening up a new pathway for precision medicine diagnostics.
Article
Microbiology
Kristy S. Syhapanha, David A. Russo, Yun Deng, Nils Meyer, Remington X. Poulin, Georg Pohnert
Summary: The discovery of an algicidal bacterium and its key protease gene provides insights into the regulation of harmful algal blooms. Transciptomics-guided approach identified a serine protease gene as a critical element in the algicidal activity. This finding opens the possibility of real-time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms.
Article
Chemistry, Multidisciplinary
Julian Hniopek, Josefine Meurer, Stefan Zechel, Michael Schmitt, Martin D. Hager, Juergen Popp
Summary: Stimuli-responsive polymers can switch specific physical properties in response to a change of the environmental conditions, making them useful for applications requiring adaptive materials. A straightforward method using Raman micro-spectroscopy and 2DCOS analysis is presented to study the response behavior of reversible polymers on a molecular level with molecular sensitivity and spatial as well as temporal resolution. This label-free and non-invasive approach allows for the investigation of macroscopic properties alongside the molecular response of the polymer to external stimuli.
Article
Chemistry, Physical
Josefine Meurer, Thomas Baetz, Julian Hniopek, Carolin Bernt, Stefan Zechel, Michael Schmitt, Juergen Popp, Martin D. Hager, Ulrich S. Schubert
Summary: This study presents a simplified synthetic procedure for rewritable shape-memory metallopolymers that also possess self-healing abilities. The formation of different orthogonal metal-complexes is used to prepare interpenetrating metallopolymer networks. The resulting IPNs exhibit excellent shape-memory abilities and self-healing efficiencies.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)