期刊
BIOFOULING
卷 26, 期 7, 页码 809-818出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2010.520159
关键词
Legionella pneumophila; biofilm; sessile growth; confocal microscopy
资金
- French Association for Thermal Spa Treatments Research (Association Francaise pour la Recherche Thermale, AFReTh)
- General Council of Les Landes
Currently, models for studying Legionella pneumophila biofilm formation rely on multi-species biofilms with low reproducibility or on growth in rich medium, where planktonic growth is unavoidable. The present study describes a new medium adapted to the growth of L. pneumophila monospecies biofilms in vitro. A microplate model was used to test several media. After incubation for 6 days in a specific biofilm broth not supporting planktonic growth, biofilms consisted of 5.36 +/- 0.40 log (cfu cm(-2)) or 5.34 +/- 0.33 log (gu cm(-2)). The adhered population remained stable for up to 3 weeks after initial inoculation. In situ confocal microscope observations revealed a typical biofilm structure, comprising cell clusters ranging up to similar to 300 mu m in height. This model is adapted to growing monospecies L. pneumophila biofilms that are structurally different from biofilms formed in a rich medium. High reproducibility and the absence of other microbial species make this model useful for studying genes involved in biofilm formation.
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