Article
Biotechnology & Applied Microbiology
Gustavo Ramos Salles Ferreira, Pollyanna Michelle da Silva, William Lopes, Ana Paula Sampaio Feitosa, Luana Cassandra Breitenbach Barroso Coelho, Fabio Andre Brayner, Luiz Carlos Alves, Patricia Maria Guedes Paiva, Maiara Celine de Moura, Marilene Henning Vainstein, Thiago Henrique Napoleao
Summary: In this study, the putative antifungal activities of pomegranate sarcotesta lectin (PgTeL) against Cryptococcus neoformans B3501 (serotype D) were investigated. The results showed that PgTeL exhibited a minimum inhibitory concentration of 172.0 μg ml(-1) and inhibited biofilm formation and disrupted preformed biofilms, indicating its potential as an anticryptococcal agent.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Mahtab Hassanpour Tazehabadi, Ammar Algburi, Igor V. Popov, Alexey M. Ermakov, Vladimir A. Chistyakov, Evgeniya V. Prazdnova, Richard Weeks, Michael L. Chikindas
Summary: Salmonellosis, caused by Salmonella, is a foodborne infection which can be transmitted from domestic poultry to humans. Probiotic bacilli, such as Bacillus subtilis and Bacillus amyloliquefaciens, show potential in controlling Salmonella infections in poultry by inhibiting biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Nay El-Khoury, Imene Bennaceur, Emilie Verplaetse, Stephane Aymerich, Didier Lereclus, Mireille Kallassy, Michel Gohar
Summary: During biofilm growth, dynamic exchanges occur between planktonic and sessile cells. The planktonic population grows until the biofilm forms, then rapidly decreases to zero, while recruited planktonic cells are found in specific areas with low sessile cell density within the biofilm, indicating spatial heterogeneity.
Article
Biotechnology & Applied Microbiology
Zhenbo Xu, Ziqi Liu, Thanapop Soteyome, Jingjing Hua, Liang Zhang, Lei Yuan, Yanrui Ye, Zhao Cai, Liang Yang, Ling Chen, Janette M. Harro, Birthe Veno Kjellerup, Junyan Liu, Yanyan Li
Summary: The study investigated the transcriptional regulation of the Cronobacter sakazakii pmrA mutant during biofilm formation, revealing important genes involved in different stages of biofilm formation.
Article
Environmental Sciences
Jia Jia, Xue Xue, Yongjing Guan, Xiaoteng Fan, Zaizhao Wang
Summary: Most bacteria in the environment form biofilms, which have inherent antibiotic tolerance. In this study, it was found that biofilm formation by Acinetobacter johnsonii is positively correlated with tetracycline resistance. Biofilms rapidly develop resistance to tetracycline when exposed to it. Persisters cells were found in both planktonic and biofilm cells, with a higher frequency in biofilms. Transcriptomic analysis revealed that planktonic cells overexpressed multidrug resistance and stress genes in response to sub-lethal concentrations of tetracycline, while biofilm cells showed lower metabolic levels, resulting in higher resistance to tetracycline. These findings suggest that A. johnsonii can adjust its phenotype and metabolism under antibiotic stress when growing as a biofilm, which has implications for biofilm control.
ENVIRONMENTAL RESEARCH
(2022)
Article
Food Science & Technology
Jinyue Sun, Lihan Huang, Zhilan Sun, Debao Wang, Fang Liu, Lihui Du, Daoying Wang
Summary: The combination of ultrasound and chlorogenic acid was shown to enhance the inactivation of Pseudomonas fluorescens planktonic and biofilm cells, quickly damaging their membrane integrity and biofilm structure.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Mayara A. R. Garcia, Reinaldo S. Theodoro, Janaina C. O. Sardi, Mariana B. Santos, Gabriela M. Ayusso, Fernando R. Pavan, Alan R. Costa, Lucas M. Santa Cruz, Pedro L. Rosalen, Luis O. Regasini
Summary: The designed and synthesized 4-bromo-3'-aminochalcone (5f) demonstrated potent antibacterial activity against methicillin-susceptible and methicillin-resistant Staphylococcus aureus, with potential for biofilm reduction and new applications as an antibacterial agent.
BIOORGANIC CHEMISTRY
(2021)
Article
Microbiology
Xiaoju Liu, Yanpeng Xiong, Yiyi Shi, Xiangbin Deng, Qiwen Deng, Yansong Liu, Zhijian Yu, Duoyun Li, Jinxin Zheng, Peiyu Li
Summary: This study found that licochalcone A has antibacterial effects on Enterococcus faecalis and significantly inhibits biofilm formation. Through genetic mutation analysis, it is suggested that licochalcone A may act by regulating transcription factor genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Hoang Minh Duc, Yu Zhang, Hoang Minh Son, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: In this study, a lytic phage STG2 capable of reducing S. enteritidis and S. typhimurium contaminations on cabbage and various surfaces was characterized. Genomic characterization revealed that phage STG2 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing both planktonic cells and biofilms on different surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Karen Hon, Sha Liu, Sophie Camens, George Spyro Bouras, Alkis James Psaltis, Peter-John Wormald, Sarah Vreugde
Summary: This study aimed to isolate and characterize phage as an alternative treatment option for multi-drug resistant E. coli infections. The newly isolated APTC-EC-2A phage showed promising properties, including stability at a broad pH range, ability to kill E. coli at different temperatures, and effectiveness against both planktonic and biofilm forms of the bacteria. Electron microscopy and genomic sequencing revealed that the phage belongs to the Myoviridae family and has lytic nature.
Article
Food Science & Technology
Bernard Gitura Kimani, Erika Beata Kerekes, Csilla Szebenyi, Judit Krisch, Csaba Vagvolgyi, Tamas Papp, Miklos Tako
Summary: Phenolic compounds exhibit significant inhibitory effects on spoilage yeasts, with cinnamic acid and vanillin showing the highest antimicrobial activity. Certain phenolics can disrupt biofilm matrix and prevent adhesion, making them potential natural interventions against food-contaminating yeasts in future preservative developments.
Article
Biotechnology & Applied Microbiology
Mohit Bansal, Nitin Dhowlaghar, Ramakrishna Nannapaneni, Divya Kode, Sam Chang, Chander S. Sharma, Christopher McDaniel, Aaron Kiess
Summary: The study demonstrates that subinhibitory concentrations of NaOCl can reduce the ability of L. monocytogenes planktonic cells to form biofilms on polystyrene surfaces and induce morphological changes in the biofilm structure and attachment capabilities. Additionally, subinhibitory NaOCl also downregulates the expression of biofilm formation-related genes in L. monocytogenes.
Article
Biotechnology & Applied Microbiology
Paula Fernandez-Gomez, Jose F. Cobo-Diaz, Marcia Oliveira, Montserrat Gonzalez-Raurich, Avelino Alvarez-Ordonez, Miguel Prieto, James L. Walsh, Morten Sivertsvik, Estefania Noriega-Fernandez, Mercedes Lopez
Summary: Plasma-Activated Water (PAW) was generated from tap water and its antimicrobial activity against Listeria monocytogenes was evaluated. PAW generated at 36 W-30 min showed the most effective inactivation, reducing the cell count by 4.6 log in planktonic state. Although the antimicrobial activity in biofilms was lower, increasing the exposure time to 30 min resulted in a reduction of >4.5 log cycles. The mechanisms of action of PAW involved changes in carbon metabolism, virulence, and stress response genes.
Article
Multidisciplinary Sciences
Ammar A. Awadh, Adam Le Gresley, Gary Forster-Wilkins, Alison F. Kelly, Mark D. Fielder
Summary: This study examined the metabolomics of two clinically important mycoplasmas using a novel approach involving nuclear magnetic resonance spectroscopy and principle component analysis. The research identified clear biofilm/planktonic differences in metabolite composition for both clinical mycoplasmas, offering insight into their survival mechanisms in different niches.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Lei Liu, Xing Chen, Xiaoyu Yin, Yao Yang, Lu Yuan, Hang Xiao, Yu Rao
Summary: This study found that the biofilm cells of a probiotic strain displayed stronger immunomodulation activity and had a more significant impact on the relative abundance of intestinal bacteria compared to their planktonic counterparts. Furthermore, the biofilm cells exhibited a stronger potential positive effect on the metabolism of the mice.
JOURNAL OF FUNCTIONAL FOODS
(2023)