Article
Biochemistry & Molecular Biology
Anika Heinze, Cara Schuldt, Sharof Khudayberdiev, Bas van Bommel, Daniela Hacker, Toni G. Schulz, Ramona Stringhi, Elena Marcello, Marina Mikhaylova, Marco B. Rust
Summary: The study identified CAP1 as a novel regulator of the postsynaptic actin cytoskeleton that is essential for synaptic cofilin1 activity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chungwon Park, Xingxiang Chen, Chong-Li Tian, Gyu Nam Park, Nicolas Chenouard, Hunki Lee, Xin Yi Yeo, Sangyong Jung, Richard W. Tsien, Guo-Qiang Bi, Hyokeun Park
Summary: Maintaining the balance between excitatory and inhibitory synaptic transmission is crucial for proper central nervous system function. Inhibitory synaptic vesicles exhibit unique dynamics and fusion properties that support rapid synaptic inhibition, showing differences compared to excitatory synaptic vesicles labeled with synaptotagmin-1. This study provides insights into the specialized mechanisms of inhibitory synaptic vesicle release, contributing to the understanding of coordinated excitation and inhibition in the CNS.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Stephanie M. Hart, Wei Jia Chen, James L. Banal, William P. Bricker, Amro Dodin, Larysa Markova, Yuliia Vyborna, Adam P. Willard, Robert Haner, Mark Bathe, Gabriela S. Schlau-Cohen
Summary: This study introduces a DNA-based platform for constructing tunable excitonic systems, allowing independent control over the coupling among the chromophores and between the chromophores and the environment. It demonstrates that the coupling between the chromophores and the environment can enhance exciton transport efficiency, pointing towards the development of designer nanophotonic devices.
Article
Biology
Kyle Aitken, Stefan Mihalas
Summary: This study investigates a network called the multi-plasticity network (MPN) that relies solely on synapse modulation during inference to process task-relevant information. The results show that the MPN outperforms recurrent neural networks (RNNs) on neuroscience-relevant tests and its computational capabilities in synaptic modulation are comparable to networks with recurrent connections.
Article
Chemistry, Multidisciplinary
Naoya Ryu, Yusei Yamamoto, Yutaka Okazaki, Nanami Hano, Yuki Iwamoto, Tomohiro Shirosaki, Shoji Nagaoka, Reiko Oda, Hirotaka Ihara, Makoto Takafuji
Summary: In this study, we achieved the chiral J-aggregation of carbocyanine dyes by utilizing lysine-derived amphiphile assemblies as scaffolds in water. The tight packing with some flexibility of the assembly promoted the formation of J-aggregates with strong chiroptical properties. Our findings emphasize the importance of molecular structure and packing in controlling the aggregation and properties of dyes.
CHEMICAL COMMUNICATIONS
(2023)
Article
Clinical Neurology
Leon A. Steiner, Andrea A. Kuhn, Jorg R. P. Geiger, Henrik Alle, Milos R. Popovic, Suneil K. Kalia, Mojgan Hodaie, Andres M. Lozano, William D. Hutchison, Luka Milosevic
Summary: This study aims to explore the local synaptic dynamics in the subthalamic nucleus (STN) and the neighboring substantia nigra pars reticulata (SNr) during external stimulation. The results show that inhibitory input in the STN persists during high frequency stimulation, while it does not occur in the SNr. This indicates a local synaptic mechanism in the STN during high frequency stimulation, which is associated with the regulation of neuronal firing.
Article
Multidisciplinary Sciences
Z. Alvarez, A. N. Kolberg-Edelbrock, I. R. Sasselli, J. A. Ortega, R. Qiu, Z. Syrgiannis, P. A. Mirau, F. Chen, S. M. Chin, S. Weigand, E. Kiskinis, S. Stupp
Summary: Researchers have successfully utilized peptide amphiphile supramolecular polymers containing two distinct signals to achieve cell signaling and tissue regeneration in a mouse model of severe spinal cord injury. By mutating the peptide sequence of the monomers, they intensified the motions of molecules within scaffold fibrils, resulting in notable effects such as vascular growth, axonal regeneration, and functional recovery. The study suggests that optimizing cell signaling through ensembles of molecules could be achieved by tuning their internal motions.
Article
Biochemical Research Methods
Yusheng Liu, Yiwei Zhang, Jiaqiang Wang, Falong Lu
Summary: Poly(A) tails are added to mRNAs in a non-templated manner and play essential roles in post-transcriptional regulation. Measuring their length is critical for understanding their regulatory roles in biological and medical studies. The PAlso-seq method utilizes PacBio sequencing to accurately measure poly(A) tail length and non-A residues, enabling analysis of samples with limited input material.
Article
Engineering, Electrical & Electronic
Rongqi Wu, Xiaochi Liu, Zhongwang Wang, Yumei Jing, Yahua Yuan, Kui Tang, Xianfu Dai, Aocheng Qiu, Hemendra N. Jaiswal, Jia Sun, Huamin Li, Jian Sun
Summary: This article demonstrates an ambipolar WSe2 synaptic FET with both electron and hole charge traps, exhibiting balanced excitation and inhibition. It achieves nearly zero asymmetry with low nonlinearity of 0.04 in long-term potentiation and depression (LTP/LTD). The proposed devices have great potential in high-accuracy neuromorphic computing applications.
IEEE ELECTRON DEVICE LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Jan Hajduch, Benjamin Fabre, Benjamin Klopp, Radek Pohl, Milos Budesinsky, Veronika Solinova, Vaclav Kasicka, Cemal Kopruluoglu, Saltuk Mustafa Eyrilmez, Martin Lepsik, Pavel Hobza, Katarina Mitrova, Marta Lubos, Maria Soledad Garre Hernandez, Jiri Jiracek
Summary: The study prepared four multi-orthogonal molecular scaffolds for synthesizing compounds mimicking the insulin receptor, with adamantane-derived compounds binding more effectively. Molecular dynamics simulations suggested that spacers provide greater flexibility for increased binding affinity.
BIOORGANIC CHEMISTRY
(2021)
Article
Cell Biology
Femmy C. Stempels, Muwei Jiang, Harry M. Warner, Magda-Lena Moser, Maaike H. Janssens, Sjors Maassen, Iris H. Nelen, Rinse de Boer, William F. Jiemy, David Knight, Julian Selley, Ronan O'Cualain, Maksim V. Baranov, Thomas C. Q. Burgers, Roberto Sansevrino, Dragomir Milovanovic, Peter Heeringa, Matthew C. Jones, Rifka Vlijm, Martin ter Beest, Geert van den Bogaart
Summary: Stempels et al. discovered large ESCRT structures in migratory cell types that wrap around clusters of integrins lacking actin. These structures are involved in membrane repair and extracellular vesicle formation. The ESCRT structures are tightly connected to the cellular support and are left behind by the cells with surrounding patches of membrane. The presence of ESCRT structures indicates membrane damage and extracellular shedding.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Neurosciences
Chao Wang, Lang Wang, Yan Gu
Summary: Microglia play a crucial role in regulating synaptic dynamics, impacting memory and forgetting, especially under pathological conditions. Uncovering their mechanisms not only helps understand the physiological functions of microglia in the brain, but also provides new strategies for preventing and treating memory impairment.
BRAIN RESEARCH BULLETIN
(2021)
Article
Chemistry, Physical
Jinfei Mei, Wen Xu, Wenqi Gao, Chuanbo Wang, Yvning Guan, Sajjad Ahmad, Hongqi Ai
Summary: The interaction between amyloid-beta 42 oligomer (A beta O) and LilrB2 D1D2 (LDD) receptor, as well as the dependence of neurotoxicity on the size and conformation of A beta O, were investigated. The study provides insights into the molecular level understanding of neurotoxicity and offers potential drug design targets for Alzheimer's disease.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Multidisciplinary Sciences
Rossana Mastrandrea, Fabrizio Piras, Andrea Gabrielli, Nerisa Banaj, Guido Caldarelli, Gianfranco Spalletta, Tommaso Gili
Summary: This study explored the robustness of brain functional architecture in schizophrenia patients and healthy controls, revealing that patients' brains are more resistant to disconnection and exhibit a unique arrangement of functional connections compared to healthy brains. The disruption of the hierarchical modularity of healthy brain organization in schizophrenia may play a crucial role in the pathogenesis of the disorder.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Christian Keine, Mohammed Al-Yaari, Tamara Radulovic, Connon Thomas, Paula Valino Ramos, Debbie Guerrero-Given, Mrinalini Ranjan, Holger Taschenberger, Naomi Kamasawa, Samuel M. Young
Summary: This study reveals the key regulatory role of Rac1 in synaptic transmission, mainly through modulating the dynamics of synaptic vesicle priming and potentially the release probability, affecting synaptic strength and short-term plasticity.
Article
Biochemistry & Molecular Biology
Amulya Nidhi Shrivastava, Virginie Redeker, Laura Pieri, Luc Bousset, Marianne Renner, Karine Madiona, Caroline Mailhes-Hamon, Audrey Coens, Luc Buee, Philippe Hantraye, Antoine Triller, Ronald Melki
Review
Neurosciences
Christian G. Specht
Review
Neurosciences
Amulya Nidhi Shrivastava, Antoine Triller, Ronald Melki
Review
Neurosciences
Xiaojuan Yang, Christian G. Specht
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Biophysics
Xianan Qin, Lei Liu, Sang Kwon Lee, Adolfo Alsina, Teng Liu, Chao Wu, Hojeong Park, Chenglong Yu, Hajin Kim, Jun Chu, Antoine Triller, Ben Zhong Tang, Changbong Hyeon, Chan Young Park, Hyokeun Park
BIOPHYSICAL JOURNAL
(2020)
Article
Biophysics
Thomas Chapdelaine, Vincent Hakim, Antoine Triller, Jonas Ranft, Christian G. Specht
Summary: Postsynaptic scaffold proteins immobilize neurotransmitter receptors at synapses for efficient transmission. Gephyrin and GlyRs form complexes at inhibitory synapses, with dynamics influenced by their interactions. This reciprocal stabilization provides quantitative insights into the organization of synaptic proteins.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Xiaojuan Yang, Herve Le Corronc, Pascal Legendre, Antoine Triller, Christian G. Specht
Summary: Super-resolution imaging has shown that GlyRs and GABA(A)Rs are dynamically organized within sub-synaptic domains at mixed inhibitory synapses, with each occupying different sub-synaptic spaces. Differential regulation of GABA(A)Rs and GlyRs, particularly in response to increased network activity, suggests that distinct signaling pathways control the clustering of these receptors at synapses.
Article
Biology
Stephanie A. Maynard, Philippe Rostaing, Natascha Schaefer, Olivier Gemin, Adrien Candat, Andrea Dumoulin, Carmen Villmann, Antoine Triller, Christian G. Specht
Summary: The study quantitatively characterized the molecular arrangement and ultra-structure of glycinergic synapses in spinal cord tissue using quantitative super-resolution correlative light and electron microscopy. The findings revealed that endogenous GlyRs exhibit equal receptor-scaffold occupancy and constant packing densities across spinal cord synapses regardless of variations in synapse size and morphology.
Article
Neurosciences
Christian G. Specht
Summary: The function of synapses depends on controlled molecular interactions, and understanding the functional role of synaptic proteins requires quantitative characterization of their behavior in the cellular environment. Single molecule localisation microscopy offers a way to obtain quantitative information about synaptic proteins at nanometer scale, providing accurate measurements of molecule concentrations. Through techniques like molecule counting, it is possible to determine absolute copy numbers of synaptic proteins and study their diffusion dynamics and binding properties in detail.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Vladimir Khayenko, Clemens Schulte, Sara L. Reis, Orly Avraham, Cataldo Schietroma, Rafael Worschech, Noah F. Nordblom, Sonja Kachler, Carmen Villmann, Katrin G. Heinze, Andreas Schlosser, Ora Schueler-Furman, Philip Tovote, Christian G. Specht, Hans M. Maric
Summary: In this study, a new probe called Sylite was developed for visualizing inhibitory synapses. Sylite showed superior performance compared to antibodies in tissue staining, and it enabled precise localization and nanoscale measurements of inhibitory synapses. Additionally, Sylite could analyze the synaptic connectivity of excitatory and inhibitory neurons in the midbrain.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Sven Wagner, ChoongKu Lee, Lucia Rojas, Christian G. Specht, JeongSeop Rhee, Nils Brose, Theofilos Papadopoulos
Summary: This study has identified the important role of GABA(A)R-alpha 3 in promoting Gephyrin-mediated and CB-independent formation of inhibitory postsynapses. It suggests that other proteins interacting with Gephyrin, besides CB, may also play a role in the formation of inhibitory synapses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Fumihiro Niwa, Angela Patrizio, Antoine Triller, Christian G. Specht
Article
Neurosciences
Nora Gruenewald, Audric Jan, Charlotte Salvatico, Vanessa Kress, Marianne Renner, Antoine Triller, Christian G. Specht, Guenter Schwarz