期刊
BIOELECTROCHEMISTRY
卷 76, 期 1-2, 页码 70-75出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.04.006
关键词
Riboflavin-binding protein electrochemistry; Mercury and carbon electrodes; Constant current chronopotentiometry; Electrocatalytic peak H; Riboflavin-protein interaction
资金
- Agency of the Czech Republic [301/07/0490, 202/07/P497]
- MEYS CR [LC06035]
- [AV0Z50040507]
- [AV0Z50040702]
Riboflavin-binding protein (RBP, a carrier of riboflavin) plays an essential role in embryo development. Electrochemical studies of the riboflavin-RBP interactions have been so far limited to changes in polarographic and voltammetric responses of riboflavin because of lack of methods capable to detect electrochemical changes in the RBP responses. Here we used constant current chronopotentiometric stripping analysis (CPSA) with the hanging mercury drop electrode (HMDE) and square wave voltammetry (SWV) with carbon paste electrode (CPE) to investigate RBP. We found that CPSA of RBP produces electrocatalytic peak H, capable to discriminate between apoprotein and holoprotein forms of RBR This peak is suitable for studies of RBP-riboflavin interaction at nanomolar concentrations. We observed no sign of a release of riboflavin from holoprotein adsorbed at the HMDE surface. SWV at CPE required higher concentrations of RBP and displayed almost identical oxidation peaks of apoprotein and holoprotein. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据