期刊
BIODEGRADATION
卷 20, 期 2, 页码 235-244出版社
SPRINGER
DOI: 10.1007/s10532-008-9216-0
关键词
Dechlorination; Dichloromethane; DCM dehalogenase; Halogenated organic compounds; Xanthobacter
资金
- European Community [HP-RTH-CT-2002-00213]
- Fundacao para a Ciencia e Tecnologia [SFRH/BPD/18716/2004]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/18716/2004] Funding Source: FCT
A bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with mu(max) of 0.094 h(-1) and S (m) of 1,435 mg l(-1). Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM.
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