Article
Chemistry, Multidisciplinary
Jong-Pil Jeon, Yu Jin Kim, Se Hun Joo, Hyuk-Jun Noh, Sang Kyu Kwak, Jong-Beom Baek
Summary: In this study, four benzotrithiophene-based covalent organic frameworks (COFs) with different conjugations were designed and synthesized, and it was found that the degree of conjugation can tune the rate of photocatalytic hydrogen production. The electron affinity and charge trapping of the electron accepting units were identified as the main factors affecting the photocatalytic performance. This research provides important insights for designing efficient photocatalysts using covalent organic frameworks.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Polymer Science
Laura Nicolle, Celine M. A. Journot, Sandrine Gerber-Lemaire
Summary: Chitosan, a natural biopolymer, has attracted great interest in research fields due to its biocompatibility, biodegradability, and mechanical properties. Various types of chemical modifications, including covalent and non-covalent methods, have been applied to improve its properties. Characterization techniques for chitosan derivatives are also important in determining their composition and physical properties.
Article
Food Science & Technology
Moting Li, Xueqi Li, David Julian McClements, Meirong Shi, Qi Shang, Xuebo Liu, Fuguo Liu
Summary: The study compared the molecular characteristics and functional properties of lactoferrin (LF)-hyaluronic acid (HA) non-covalent complexes and conjugates formed by physical mixing and chemical conjugation. It was found that covalently attaching HA to LF significantly improved its emulsifying capacity and antioxidant capacity, but did not improve its foaming properties. This suggests that forming complexes or conjugates with HA could enhance the functional performance of LF in various food applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Carina Hey Pui Cheung, Tin Hang Chong, Tongyao Wei, Han Liu, Xuechen Li
Summary: Recently, ortho-phthalaldehyde (OPA) has been used for the modification of proteins and peptides through OPA-amine two-component reactions and intramolecular OPA-amine-thiol three-component reactions. Guanidine has been discovered to be an effective additive in switching the intermolecular OPA-amine-thiol three-component reaction to a stoichiometric process, enabling the construction of peptide-peptide and peptide-drug conjugate structures. This reaction shows great versatility and flexibility, as demonstrated by the successful synthesis of model peptide-peptide and peptide-drug conjugates using unprotected peptides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Wendy Cao, Johnathan C. Maza, Natalia Chernyak, John A. Flygare, Shane W. Krska, F. Dean Toste, Matthew B. Francis
Summary: Cysteines are commonly used as handles to synthesize antibody-drug conjugates, and tyrosinase can be used to attach phenolic cargo to cysteines engineered on antibody surfaces. This method provides more stable linkages and can be used for constructing antibody conjugates with cytotoxic payloads and attaching various cargos.
BIOCONJUGATE CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Luis H. Gutierrez-Gonzalez, Selma Rivas-Fuentes, Silvia Guzman-Beltran, Angelica Flores-Flores, Jorge Rosas-Garcia, Teresa Santos-Mendoza
Summary: PDZ-dependent interactions are widely distributed in different cell types and regulated various cellular processes. Targeting these interactions pharmacologically could help control immune-related diseases. Further understanding of these interactions may lead to the discovery of novel pharmacological targets for a variety of clinical conditions.
Article
Multidisciplinary Sciences
Nikolaj Riis Christensen, Christian Parsbok Pedersen, Vita Sereikaite, Jannik Nedergaard Pedersen, Maria Vistrup-Parry, Andreas Toft Sorensen, Daniel Otzen, Kaare Teilum, Kenneth Lindegaard Madsen, Kristian Stromgaard
Summary: The organization of the postsynaptic density (PSD) is mediated by specific protein-protein interactions (PPIs) and plays a crucial role in synaptic transmission. PSD-95 interacts with various proteins, including TARPs, and this study reveals the essential peptide responsible for the liquid-liquid phase separation (LLPS) formation of PSD-95 and other key protein constituents of the PSD. The study also highlights the role of affinity and multivalency in LLPS systems.
Article
Biochemical Research Methods
Chang Hoon Jeon, Tai Hwan Ha
Summary: We reported a strategy of immobilizing antibodies using photoactivatable Fc-binding RNA aptamers (FcBAs) in a covalent and well-oriented manner. We prepared modified FcBAs with photoaffinity probes and evaluated their binding capabilities and photo-crosslinking efficiency. The initial efficiency was improved by multivalent association and structural modification of FcBAs. We demonstrated the applicability of this strategy in immunoassays through the fabrication and investigation of covalent crosslinking on solid chips.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sergio Celis, Fruzsina Hobor, Thomas James, Gail J. Bartlett, Amaurys A. Ibarra, Deborah K. Shoemark, Zsofia Hegedus, Kristina Hetherington, Derek N. Woolfson, Richard B. Sessions, Thomas A. Edwards, David M. Andrews, Adam Nelson, Andrew J. Wilson
Summary: The study presents a general approach to discover orthosteric PPI inhibitors that mimic specific secondary protein structures, validated through experimental examples. Low μM activity selective PPI inhibitors were discovered for two unrelated PPIs, with defined structure-activity relationships established through hit expansion. The approach is believed to have the potential to enable the discovery of inhibitors for a wide range of unrelated secondary structure-mediated PPIs.
Review
Chemistry, Multidisciplinary
Yang Yue, Daizong Ji, Yunqi Liu, Dacheng Wei
Summary: Covalent organic frameworks (COFs) are crystalline porous polymers composed of light elements through strong covalent bonds. They have unique designable structures and excellent material properties, making them highly promising for various applications. This review focuses on COF-based sensors, discussing their fundamental principles, primary application areas, recent advancements, and future trends and challenges in sensing. COFs offer numerous potential in addressing crucial points for sensing application, thanks to their high surface area, tunable polygonal porosity, predictable and stable structure, and precise chemical control.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Ahmed E. Fouda, Aparni K. Gamage, Mary Kay H. Pflum
Summary: A new affinity-based crosslinking ATP analog, ATP-MAc, has been developed to covalently link kinases containing cysteines in the active site with their substrates. This method avoids the challenges posed by UV light irradiation and non-specific reactivity, providing a new tool for studying signaling cascades in normal and diseased states.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Qiaozhi Zhang, Zhouzhou Cheng, Ruyan Chen, Yanbo Wang, Song Miao, Zhenxing Li, Shunyu Wang, Linglin Fu
Summary: This study systematically investigated the interactions between cyanidin-3-O-glucoside and two major milk proteins, demonstrating that covalent complexes reduce protein digestibility while exhibiting antioxidant properties and lower allergenicity compared to their non-covalent counterparts.
Article
Biotechnology & Applied Microbiology
Shiyu Chen, Scott Lovell, Sumin Lee, Matthias Fellner, Peter D. Mace, Matthew Bogyo
Summary: A phage display method was developed to screen for covalent inhibitors that bind to protein targets by introducing a reactive linker to form cyclic peptides and an electrophilic 'warhead' to react with target nucleophiles. This approach identified cyclic peptides that irreversibly inhibited cysteine protease and serine hydrolase with high specificity and potency. It enables rapid, unbiased screening to identify new classes of highly selective covalent inhibitors for diverse molecular targets.
NATURE BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Xiaohan Chen, Youwen Zhang, Pearl Arora, Xiyun Guan
Summary: Different species can be detected using nanopores engineered with various recognition sites based on non-covalent interactions. This strategy allows for differentiation between species, making it potentially useful for molecular detection in medical and environmental applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Yunfei Wang, Kai-Lin Chen, Angela Awada, Nathaniel Prine, Zhiqiang Cao, Chenhui Zhu, Yu-Cheng Chiu, Simon Rondeau-Gagne, Xiaodan Gu
Summary: This study demonstrates a method to control the phase separation size of CP/elastomer composites by strategically controlling the location sites of H-bonding functional groups in CPs and elastomers, and investigates their effects on mechanical and electrical properties.
CHEMISTRY OF MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Li Duan, Xiao Xu, Limei Xu, Hongfei Chen, Xingfu Li, Murad Alahdal, Yin Xiao, Yujie Liang, Jiang Xia
Summary: Osteoarthritis is a degenerative disease that affects the quality of life in the elderly population, with no ideal treatment strategies currently available. Studies have suggested that exosomes may play an important role in the treatment of osteoarthritis.
CURRENT MEDICINAL CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Yujie Liang, Xiao Xu, Limei Xu, Indira Prasadam, Li Duan, Yin Xiao, Jiang Xia
Summary: Intra-articular drug delivery is a conservative treatment method that improves the bioavailability of therapeutic composites in OA joint cavity. New targeted nano-based drug delivery systems and gene delivery for OA therapy show promising prospects.
JOURNAL OF DRUG TARGETING
(2021)
Review
Pharmacology & Pharmacy
Cheng Wang, Tingting Hong, Pengfei Cui, Jianhao Wang, Jiang Xia
Summary: This article reviews recent studies on the delivery and formulation of antimicrobial peptides, categorizing strategies driven by physical interactions or chemical conjugation reactions, and covering carriers from inorganic solid nanoparticles to organic micelles, liposomes, and hydrogels. Targeted delivery, responsive release, and strategies to increase oral bioavailability of antimicrobial peptides are discussed, highlighting the recent progress towards clinical application.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Biochemical Research Methods
Ruidi Wang, Miao Liu, Han Wang, Jiang Xia, Hongbin Li
Summary: Developing peptide tags that can bind target proteins covalently under mild conditions is crucial for various applications. The GB tags developed in this study show high specificity and yield in covalently labeling target proteins under oxidizing conditions, with improved thermodynamic stability and binding affinity. These new GB tags have potential applications in biochemical labeling and biomaterials such as protein hydrogels.
BIOCONJUGATE CHEMISTRY
(2021)
Review
Cell & Tissue Engineering
Jianghong Huang, Jun Zhang, Jianyi Xiong, Shuqing Sun, Jiang Xia, Lei Yang, Yujie Liang
Summary: Wound healing and regeneration are dynamic processes that involve collaboration between various factors. Stem cell-derived nanovesicles, such as microvesicles and exosomes, play important roles in promoting wound healing by delivering biologically active substances to regulate cellular activity in the wound area.
STEM CELLS INTERNATIONAL
(2021)
Article
Chemistry, Multidisciplinary
Yue Wang, Min Liu, Qixin Wei, Wanjie Wu, Yanping He, Jiayang Gao, Renjie Zhou, Liwen Jiang, Jianan Qu, Jiang Xia
Summary: This study demonstrates the construction of synthetic multienzyme condensates through LLPS, which significantly enhances the biosynthesis of α-farnesene.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Qixin Wei, Yue Wang, Zhenjun Liu, Min Liu, Sheng Cao, Hao Jiang, Jiang Xia
Summary: Assembling enzymes into complexes increases production efficiency and yield of desired products during biosynthesis. This study demonstrates the construction of membrane-bound multienzyme complexes, which can independently produce specific products both in vitro and in vivo.
Article
Chemistry, Multidisciplinary
Dingdong Yuan, Yu Zhang, King Hoo Lim, Stephen King Pong Leung, Xizi Yang, Yujie Liang, Wilson Chun Yu Lau, Kwan T. Chow, Jiang Xia
Summary: This study reports a peptide-guided, proximity-driven group transfer reaction for site-selective acetylation of a single lysine residue in IgG. The reaction proceeded efficiently under physiological conditions without the need for deglycosylation or catalysts, allowing further derivatization of IgG. The method was also used to synthesize antibody-lipid conjugates and antibody-conjugated liposomes for targeted cancer cell therapy. Additionally, a bispecific antibody complex showed potent in vitro cytotoxicity at nanomolar concentrations.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biotechnology & Applied Microbiology
Yujie Liang, Zoya Iqbal, Jianping Lu, Jianhong Wang, Hao Zhang, Xi Chen, Li Duan, Jiang Xia
Summary: Developing strategies for safe and effective drug delivery into the CNS is crucial. Cell-derived nanovesicles (CDNs), particularly extracellular vesicles and exosomes, show promise as CNS-targeted drug carriers due to their stability, biocompatibility, homing ability, and ability to penetrate biological barriers. In this review, we focus on the application of CDNs in brain-targeted drug delivery, including recent methods of exosome derivation and engineering. We discuss the limitations and future development of CDN-based brain-targeted delivery systems, highlighting their potential in treating neurological diseases.
Review
Biochemistry & Molecular Biology
Min Liu, Yue Wang, Hao Jiang, Yongxu Han, Jiang Xia
Summary: In nature, enzymes that catalyze sequential reactions are often formed as clusters or complexes. Synthetic versions of these multienzyme complexes have been developed to enhance catalytic rates and improve microbial production of valuable chemicals. These complexes optimize metabolic fluxes, increase product yield, and have the potential for large-scale industrial applications. However, there is still a need for strategies to balance dynamicity and confinement, and achieve better control of local compartmentalization, similar to natural multienzyme complexes.
Article
Nanoscience & Nanotechnology
Biao Wang, Donghui Zhao, Yuting Li, Xinpei Zhou, Zexuan Hui, Xiaoling Lei, Lin Qiu, Yang Bai, Cheng Wang, Jiang Xia, Yang Xuan, Pengju Jiang, Jianhao Wang
Summary: In order to prevent the development of drug resistance during antibiotic treatment of wound infections, the use of antimicrobial peptides (AMPs) has been explored. However, challenges such as low bioavailability, rapid degradation of AMPs, and the difficulty of eliminating bacteria in deep tissue hinder its application. To address these issues, microneedle patches containing AMPs and recombinant type III humanized collagen (Col III) were designed for slow and controlled release to effectively eradicate bacteria in deep wound tissue and promote wound healing. This strategy demonstrates the potential of a microneedle-based antimicrobial drug delivery system.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yishu Bao, Hongfei Chen, Zhiyi Xu, Jiayang Gao, Liwen Jiang, Jiang Xia
Summary: The article describes a new fluorescent molecule that can deliver proteins into mammalian cells and release the payload into the cytosol through photoinduced disintegration.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Materials Science, Biomaterials
Zoya Iqbal, Khurrum Rehman, Jiang Xia, Maryam Shabbir, Muhammad Zaman, Yujie Liang, Li Duan
Summary: RISPR-Cas9 has great potential in gene therapy, but its application is hindered by limited delivery methods. Biomaterial-based drug delivery systems can overcome these challenges and provide precise gene editing with reduced side effects. This review discusses the current status and progress of CRISPR/Cas9 delivery approaches, including polymeric nanoparticles, liposomes, extracellular vesicles, inorganic nanoparticles, and hydrogels. Targeted delivery vehicles for CRISPR systems are also highlighted.
BIOMATERIALS SCIENCE
(2023)
Review
Materials Science, Biomaterials
Yujie Liang, Zoya Iqbal, Jianhong Wang, Limei Xu, Xiao Xu, Kan Ouyang, Hao Zhang, Jianping Lu, Li Duan, Jiang Xia
Summary: Genome editing technology has the potential to treat incurable diseases, but the development of delivery systems is lagging behind. Extracellular vesicles (EVs), as natural nanocarriers, have advantages of biocompatibility and stability, making them a promising drug delivery vehicle.
BIOMATERIALS SCIENCE
(2022)
Article
Materials Science, Biomaterials
Jianghong Huang, Zhiwang Huang, Yujie Liang, Weihao Yuan, Liming Bian, Li Duan, Zhibin Rong, Jianyi Xiong, Daping Wang, Jiang Xia
Summary: This study developed gelatin/hydroxyapatite hybrid materials as a scaffold for human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) through microextrusion 3D bioprinting and enzymatic cross-linking. Doping HAP in the gelatin scaffold improved the fluidity of the hydrogel and allowed better control over 3D printing. Implanting the hUCB-MSC-laden scaffold effectively repaired cartilage defects in a pig model, demonstrating the potential of gelatin-based scaffold materials for hUCB-MSCs to treat articular cartilage injury.
BIOMATERIALS SCIENCE
(2021)