期刊
BIOCONJUGATE CHEMISTRY
卷 25, 期 8, 页码 1395-1401出版社
AMER CHEMICAL SOC
DOI: 10.1021/bc5002467
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资金
- UCL
- Wellcome Trust
- BBSRC
- UCLB
- KCL and UCL Comprehensive Cancer Imaging Centre (CCIC) [C1519/A10331]
- Department of Health and Cancer Research UK Experimental Cancer Medicine Centre (ECMC) [C34/A7279]
- EU [259881]
- BBSRC [BB/J010448/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J010448/1] Funding Source: researchfish
We report on a chemical platform to generate site-specific, homogeneous, antibody-antibody conjugates by targeting and bridging disulfide bonds. A bispecific antibody construct was produced in good yield through simple reduction and bridging of antibody fragment disulfide bonds, using a readily synthesized bis-dibromomaleimide cross-linker. Binding activity of antibodies was maintained, and in vitro binding of target antigens was observed. This technology is demonstrated through linking scFv and Fab antibody fragments, showing its potential for the construction of a diverse range of bispecifics.
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