Article
Medicine, Research & Experimental
Yoann Aldon, Paul F. McKay, Jorge Moreno Herrero, Annette B. Vogel, Reka Levai, Pauline Maisonnasse, Nathalie Dereuddre-Bosquet, Heinrich Haas, Katalin Fabian, Roger Le Grand, Ugur Sahin, Robin J. Shattock
Summary: saRNA platform, particularly VEEV saRNA, shows potential for high protein expression and strong immune responses in delivering stabilized HIV-1 Env. Membrane-bound Env design leads to faster immune kinetics and better IgG responses, with promise for diverse vaccine regimens.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Review
Biotechnology & Applied Microbiology
Ezio Ricca, Loredana Baccigalupi, Rachele Isticato
Summary: Surface display systems have been developed to express target molecules on various biological entities and synthetic particles for technological and biomedical applications. The non-recombinant approach to display antigens and enzymes on bacterial spores shows potential applications in various fields.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Pharmacology & Pharmacy
David Peterhoff, Stefanie Thalhauser, Patrick Neckermann, Clara Barbey, Kristina Straub, Julian Nazet, Rainer Merkl, Gernot Laengst, Miriam Breunig, Ralf Wagner
Summary: Selective targeting of germline B cells with specifically designed germline-targeting HIV-1 envelope immunogens presented on the surface of silica nanoparticles can enhance the affinity to B cells and induce the generation of broadly neutralizing antibodies. Covalent conjugation of GT-Env exhibits better binding capability to structure-dependent bnAbs compared to non-specifically adsorbed GT-Env. The use of nanoparticle delivery allows activation of B cells at lower concentrations, likely due to avidity effects.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Instruments & Instrumentation
Maria Plaza-Oliver, Manuel Jesus Santander-Ortega, Maria Victoria Lozano
Summary: Lipid-based nanocarriers have advantages like low toxicity and strong biocompatibility, making them ideal carriers for drugs with poor oral bioavailability. However, efficient oral drug delivery through these carriers is challenging due to multiple physicochemical barriers along the gastrointestinal tract. Rational design of nanocarriers can turn these limitations into opportunities, ultimately determining their future clinical success.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Guilherme M. Lima, Alexey Atrazhev, Susmita Sarkar, Mirat Sojitra, Revathi Reddy, Karin Torres-Obreque, Carlota de Oliveira Rangel-Yagui, Matthew S. Macauley, Gisele Monteiro, Ratmir Derda
Summary: This study demonstrates a method for displaying large multisubunit proteins on phages by connecting different fusion proteins to phage clones displaying peptides. The research findings suggest that different valencies and chemical modifications can affect the clearance rate of proteins in vivo.
ACS CHEMICAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
M. M. Jeevani Osadee Wijekoon, Kaiser Mahmood, Fazilah Ariffin, Abdorreza Mohammadi Nafchi, Musfirah Zulkurnain
Summary: Fat-soluble vitamins are important nutrients in human nutrition but are susceptible to degradation due to environmental conditions. This review discusses different encapsulation formulations of fat-soluble vitamins in biopolymer and lipid-based carriers for applications in food and pharmaceutical industries, emphasizing on encapsulation efficiency, stability, bioavailability, and bio-accessibility. Future research should focus on the effects of different vitamin types and encapsulate sizes on bioaccessibility and biocompatibility, as well as the toxicity and safety evaluation of encapsulated fat-soluble vitamins in human health.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Alexandra Suberi, Molly K. Grun, Tianyang Mao, Benjamin Israelow, Melanie Reschke, Julian Grundler, Laiba Akhtar, Teresa Lee, Kwangsoo Shin, Alexandra S. Piotrowski-Daspit, Robert J. Homer, Akiko Iwasaki, Hee-Won Suh, W. Mark Saltzman
Summary: An inhalable polymer-based vehicle has been developed for the delivery of therapeutic mRNA to the lungs, achieving high transfection efficiency in epithelial and antigen-presenting cells.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Pei Kun Richie Tay, Pei Yu Lim, Dave Siak-Wei Ow
Summary: Lactic acid bacteria can display functional proteins on their cell surface using the cell anchoring domain CAD4a, such as the antioxidant enzyme SOD, which shows potential for treating gut inflammation. CAD4a binds non-covalently to peptidoglycan in the bacterial cell wall, allowing high stability and uniform anchoring of proteins.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Demi L. Pink, Fabrizia Foglia, David J. Barlow, M. Jayne Lawrence, Christian D. Lorenz
Summary: This study characterized the structure of 2C6PC micelles using various methods and gained a detailed understanding of the aggregation behavior of substrate and product during degradation, revealing the formation of mixed micelles at each stage. Atomistic molecular dynamics allowed for the localization of different molecular species within aggregates and documented the hydration of the species, shedding light on the processes driving their micellization.
Article
Materials Science, Biomaterials
Maria Lina Formica, Samuel Legeay, Jerome Bejaud, Guillermo Gabriel Montich, Gabriela Veronica Ullio Gamboa, Jean-Pierre Benoit, Santiago Daniel Palma
Summary: The study aimed to develop a novel hybrid formulation based on lipid nanocapsules containing bevacizumab and triamcinolone acetonide for improved ocular therapy, showing potential in treating eye disorders related to inflammation and neovascularization.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Pharmacology & Pharmacy
Yuan-Shuo Hsueh, Yan-Jye Shyong, Hsiu-Ching Yu, Shu-Jhen Jheng, Shang-Wen Lin, Hua-Lin Wu, Jui-Chen Tsai
Summary: The study developed NLC formulations containing rhTM, which effectively promoted chronic wound healing with high encapsulation efficiency and sustained release. In diabetic mouse models, it showed significant improvement in wound healing and cell migration, with lower plasma concentrations compared to rhTM solution.
Article
Chemistry, Physical
Demi L. Pink, Orathai Loruthai, Robert M. Ziolek, Ann E. Terry, David J. Barlow, M. Jayne Lawrence, Christian D. Lorenz
Summary: Liquid lipid nanoparticles (LLNs) are nanoemulsions with oil-in-water structure for delivery of hydrophobic drug molecules. The internal structure of LLNs, crucial for stable drug delivery vehicles, is studied using machine learning techniques, neutron scattering experiments, and molecular dynamics simulations, revealing the formation of disordered lipid core with the addition of surfactants. Unsupervised artificial neural networks demonstrate superior ability in characterizing the internal structure of LLNs compared to conventional geometric methods, providing a multi-scale picture of the dominance of lipid and surfactant conformations.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Medicine, Research & Experimental
Daniela Staquicini, E. Magda Barbu, Rachel L. Zemans, Beth K. Dray, Fernanda Staquicini, Prashant Dogra, Marina Cardo-Vila, Cindy K. Miranti, Wallace B. Baze, Luisa L. Villa, Jorge Kalil, Geetanjali Sharma, Eric R. Prossnitz, Zhihui Wang, Vittorio Cristini, Richard L. Sidman, Andrew R. Berman, Reynold A. Panettieri, Rubin M. Tuder, Renata Pasqualini, Wadih Arap
Summary: This study utilized the lungs as a route for drug delivery and developed a method for systemic immune targeting vaccination through ligand-directed pulmonary delivery. By screening a phage display peptide library, a specific targeting ligand was identified, leading to successful pulmonary delivery of targeted phage particles in animals, eliciting systemic and specific immune responses.
Article
Cell Biology
Etsuro Nanishi, Francesco Borriello, Timothy R. O'Meara, Marisa E. McGrath, Yoshine Saito, Robert E. Haupt, Hyuk-Soo Seo, Simon D. van Haren, Cecilia B. Cavazzoni, Byron Brook, Soumik Barman, Jing Chen, Joann Diray-Arce, Simon Doss-Gollin, Maria De Leon, Alejandra Prevost-Reilly, Katherine Chew, Manisha Menon, Kijun Song, Andrew Z. Xu, Timothy M. Caradonna, Jared Feldman, Blake M. Hauser, Aaron G. Schmidt, Amy C. Sherman, Lindsey R. Baden, Robert K. Ernst, Carly Dillen, Stuart M. Weston, Robert M. Johnson, Holly L. Hammond, Romana Mayer, Allen Burke, Maria E. Bottazzi, Peter J. Hotez, Ulrich Strych, Aiquan Chang, Jingyou Yu, Peter T. Sage, Dan H. Barouch, Sirano Dhe-Paganon, Ivan Zanoni, Al Ozonoff, Matthew B. Frieman, Ofer Levy, David J. Dowling
Summary: The global deployment of vaccines for COVID-19 is urgently needed, especially in low- and middle-income countries. The study found that a specific adjuvant formulation significantly increased neutralizing antibody levels and protected aged mice from SARS-CoV-2. These findings support the further development of this adjuvanted vaccine as an effective and affordable option.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
John D. Ventura, Joseph P. Nkolola, Abishek Chandrashekar, Erica N. Borducchi, Jinyan Liu, Noe B. Mercado, David L. Hope, Victoria M. Giffin, Katherine McMahan, Romas Geleziunas, Jeffrey P. Murry, Yunling Yang, Mark G. Lewis, Maria G. Pau, Frank Wegmann, Hanneke Schuitemaker, Emily J. Fray, Mithra R. Kumar, Janet D. Siliciano, Robert F. Siliciano, Merlin L. Robb, Nelson L. Michael, Dan H. Barouch
Summary: This study investigated the therapeutic efficacy of an Ad26/MVA vaccine with vesatolimod in ART-suppressed, SIVmac251-infected rhesus macaques. The vaccine led to robust immune responses and virologic control was observed after discontinuation of ART. SIV-specific cellular immune responses correlated with virologic control.
Editorial Material
Multidisciplinary Sciences
E. John Wherry, Dan H. Barouch
Article
Immunology
Daniel J. Stieh, Dan H. Barouch, Christy Comeaux, Michal Sarnecki, Kathryn E. Stephenson, Stephen R. Walsh, Sheetal Sawant, Jack Heptinstall, Georgia D. Tomaras, James G. Kublin, M. Juliana McElrath, Kristen W. Cohen, Stephen C. De Rosa, Galit Alter, Guido Ferrari, David Montefiori, Philipp Mann, Steven Nijs, Katleen Callewaert, Paul A. Goepfert, Srilatha Edupuganti, Etienne Karita, Michael S. Seaman, Lawrence Corey, Lindsey R. Baden, Maria G. Pau, Hanneke Schuitemaker, Frank Tomaka
Summary: This study developed a cross-clade, globally effective HIV vaccine through clinical trials on healthy individuals with low risk for HIV infection. The findings showed that adding mosaic gp140 to the vaccine regimen increased and broadened the elicited immune response without compromising safety or clade C responses.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Letter
Medicine, General & Internal
Ai-ris Y. Collier, Jessica Miller, Nicole P. Hachmann, Katherine McMahan, Jinyan Liu, Esther A. Bondzie, Lydia Gallup, Marjorie Rowe, Eleanor Schonberg, Siline Thai, Julia Barrett, Erica N. Borducchi, Emily Bouffard, Catherine Jacob-Dolan, Camille R. Mazurek, Audrey Mutoni, Olivia Powers, Michaela Sciacca, Nehalee Surve, Haley VanWyk, Cindy Wu, Dan H. Barouch
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Letter
Medicine, General & Internal
Jessica Miller, Nicole P. Hachmann, Ai-ris Y. Collier, Ninaad Lasrado, Camille R. Mazurek, Robert C. Patio, Olivia Powers, Nehalee Surve, James Theiler, Bette Korber, Dan H. Barouch
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Multidisciplinary Sciences
Jennifer Cable, Barney S. Graham, Richard A. Koup, Robert A. Seder, Katalin Kariko, Norbert Pardi, Dan H. Barouch, Bhawna Sharma, Susanne Rauch, Raffael Nachbagauer, Mattias N. E. Forsell, Michael Schotsaert, Ali H. Ellebedy, Karin Lore, Darrell J. Irvine, Emily Pilkington, Siri Tahtinen, Elizabeth A. Thompson, Yanis Feraoun, Neil P. King, Kevin Saunders, Galit Alter, Syed M. Moin, Kwinten Sliepen, Gunilla B. Karlsson Hedestam, Hedda Wardemann, Bali Pulendran, Nicole A. Doria-Rose, Wan-Ting He, Jennifer A. Juno, Sila Ataca, Adam K. Wheatley, Jason S. McLellan, Laura M. Walker, Julia Lederhofer, Lisa C. Lindesmith, Holger Wille, Peter J. Hotez, Linda-Gail Bekker
Summary: The COVID-19 pandemic has highlighted the importance of vaccines in public health and advancements in vaccine technology are vital for preventing infectious diseases. While many diseases still lack effective vaccines, recent developments in fields like immunology and structural biology may lead to breakthroughs in vaccination and enhance preparedness for future pandemics.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Obstetrics & Gynecology
Ai-ris Y. Collier, Anna M. Modest, Ricardo A. Aguayo, Esther A. Bondzie, Shivani Patel, Michele R. Hacker, Dan H. Barouch
Summary: This study aimed to investigate the differences in T cell phenotype and function in uterine intervillous blood (IVB) between normal pregnancy and preeclampsia, and to explore the immunologic basis of preeclampsia. The results showed that preeclampsia was associated with a greater pro-inflammatory cytokine ratio in uterine IVB T cell subsets, indicating an inflammation-related mechanism of preeclampsia. Therefore, IVB sampling is a useful tool for studying human uterine T cell biology and may provide insights for immunotherapeutic strategies for preeclampsia.
REPRODUCTIVE SCIENCES
(2023)
Correction
Immunology
Etsuro Nanishi, Francesco Borriello, Hyuk-Soo Seo, Timothy R. O'Meara, Marisa E. McGrath, Yoshine Saito, Jing Chen, Joann Diray-Arce, Kijun Song, Andrew Z. Xu, Soumik Barman, Manisha Menon, Danica Dong, Timothy M. Caradonna, Jared Feldman, Blake M. Hauser, Aaron G. Schmidt, Lindsey R. Baden, Robert K. Ernst, Carly Dillen, Jingyou Yu, Aiquan Chang, Luuk Hilgers, Peter Paul Platenburg, Sirano Dhe-Paganon, Dan H. Barouch, Al Ozonoff, Ivan Zanoni, Matthew B. Frieman, David J. Dowling, Ofer Levy
Article
Immunology
Etsuro Nanishi, Francesco Borriello, Hyuk-Soo Seo, Timothy R. O'Meara, Marisa E. McGrath, Yoshine Saito, Jing Chen, Joann Diray-Arce, Kijun Song, Andrew Z. Xu, Soumik Barman, Manisha Menon, Danica Dong, Timothy M. Caradonna, Jared Feldman, Blake M. Hauser, Aaron G. Schmidt, Lindsey R. Baden, Robert K. Ernst, Carly Dillen, Jingyou Yu, Aiquan Chang, Luuk Hilgers, Peter Paul Platenburg, Sirano Dhe-Paganon, Dan H. Barouch, Al Ozonoff, Ivan Zanoni, Matthew B. Frieman, David J. Dowling, Ofer Levy
Summary: The development of vaccines that protect vulnerable populations from SARS-CoV-2 is a public health priority. This study tested different adjuvants and SARS-CoV-2 antigens to identify a combination that induces antibodies and protection in both young and aged mice. An oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMS:O/W) was found to be the most effective in enhancing immune response and protection across age groups.
Article
Multidisciplinary Sciences
Samuel J. Vidal, Daniel Sellers, Jingyou Yu, Shoko Wakabayashi, Jaimie Sixsmith, Malika Aid, Julia Barrett, Sage F. Stevens, Xiaowen Li, Wenjun Li, Courtney R. Plumlee, Kevin B. Urdahl, Amanda J. Martinot, Dan H. Barouch
Summary: This study found that the live attenuated Mycobacterium tuberculosis vaccine Delta LprG provided better protection compared to BCG in a low-dose murine aerosol challenge model, preventing infection establishment and dissemination and confining the infection to one lung. The protection was partially influenced by certain cytokines.
Article
Cell Biology
Jingyou Yu, Paul V. Thomas, Michaela Sciacca, Cindy Wu, Jinyan Liu, Xuan He, Jessica Miller, Nicole P. Hachmann, Nehalee Surve, Katherine McMahan, Catherine Jacob-Dolan, Olivia Powers, Kevin Hall, Julia Barrett, David Hope, Camille R. Mazurek, Tetyana Murdza, William C. Chang, Emily Golub, Phyllis A. Rees, Caroline E. Peterson, Agnes Hajduczki, Wei-Hung Chen, Elizabeth J. Martinez, Elizabeth Hussin, Camille Lange, Hua Gong, Gary R. Matyas, Mangala Rao, Mehul Suthar, Mona Boursiquot, Anthony Cook, Laurent Pessaint, Mark G. Lewis, Hanne Andersen, Diane L. Bolton, Nelson L. Michael, M. Gordon Joyce, Kayvon Modjarrad, Dan H. Barouch
Summary: SARS-CoV-2 vaccines have reduced protection against the acquisition of the BA.5 subvariant, but remain effective against severe disease. The immune correlates of protection against BA.5 are unknown. This study demonstrates that both homologous and heterologous regimens involving Ad26.COV2.S and SpFN vaccines provide robust protection against a mismatched BA.5 challenge in macaques.
CELL REPORTS MEDICINE
(2023)
Article
Cell Biology
Malika Aid, Alessandro Colarusso, Victoria Walker-Sperling, Dan H. Barouch
Summary: The discovery of biomarkers that predict viral rebound after discontinuation of antiretroviral therapy (ART) would significantly contribute to the HIV cure field.
CELL REPORTS MEDICINE
(2023)
Meeting Abstract
Veterinary Sciences
Raja Mohan Gopalakrishnan, Malika Aid, Noe B. Mercado, Caitlin Davis, Shaily Malik, Valerie Varner, Rhianna Jones, Steven E. Bosinger, Cesar Piedra-Mora, Amanda J. Martinot, Dan H. Barouch, R. Keith Reeves, C. Sabrina Tan
JOURNAL OF MEDICAL PRIMATOLOGY
(2022)
Meeting Abstract
Veterinary Sciences
Jeffrey Schneider, Flora Engelmann, Ann Carias, Billy Nguyen, MIchelle Ash, Dan Barouch, Ron Veazey, Thomas Hope
JOURNAL OF MEDICAL PRIMATOLOGY
(2022)