Review
Biotechnology & Applied Microbiology
Yujia Xu, Michele Kirchner
Summary: Collagen mimetic peptides (CMPs) have been utilized since the late 1960s as a molecular tool for studying collagen and developing novel collagen mimetic biomaterials. Recent advancements have focused on producing heterotrimeric CMPs and CMPs capable of forming collagen-like higher order molecular assemblies. The interdisciplinary field of CMP research has seen significant growth and progress in understanding collagen structure and properties.
BIOENGINEERING-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Seyed Mohammad Siadat, Alexandra A. Silverman, Monica E. Susilo, Jeffrey A. Paten, Charles A. DiMarzio, Jeffrey W. Ruberti
Summary: This study focuses on the growth and remodeling of extant collagen fibrils, and demonstrates that labeling collagen molecules with small fluorophores can effectively address the observation-interference problem while preserving the characteristics of the fibrils.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Maryam Sanaee, Elin Sandberg, K. Goran Ronquist, Jane M. Morrell, Jerker Widengren, Katia Gallo
Summary: Exosomes and exosome-like nanovesicles have the potential to serve as drug delivery carriers due to their targeting functionality and low immunogenicity. However, accurate methods to load them and characterize their contents are challenging due to their small size, polydispersity, and aggregation in solution. In this study, a methodology based on burst analysis of dual-color confocal fluorescence microscopy experiments is developed to quantitatively characterize these nanovesicles and their fluorescently-labeled cargo. Successful loading of nanovesicles derived from animal extracellular vesicles and human red blood cell detergent-resistant membranes is demonstrated, and size statistics and loading yields are quantified using dual-color coincident fluorescence burst analysis.
Article
Pharmacology & Pharmacy
Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, Brian P. Ceresa, David J. Calkins
Summary: The cornea, a highly collagenous structure with multiple layers, is at risk for injury due to constant exposure to the external environment. A study found that a collagen mimetic peptide (CMP) can promote epithelial healing following acute corneal wounds by repairing damaged collagen. This suggests that CMPs may represent a novel therapeutic approach for healing corneal tissue in conditions that damage the ocular surface.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Nolan R. McGrady, Silvia Pasini, Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, David J. Calkins
Summary: Optic neuropathies, particularly glaucoma, are a significant cause of vision loss worldwide. Current treatments focus on lowering intraocular pressure, but there is a need for new therapeutics targeting neurodegeneration directly. Collagen mimetic peptides have shown promising results in protecting retinal ganglion cell axons, potentially serving as a novel therapeutic option.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Medicinal
Rie Kamihira, Yoichi Nakao
Summary: In this study, fluorescently labeled chemical probes were prepared from cytotoxic marine cyclic peptides to visualize their localization in cells. The results showed that Kap A-5-FL had strong staining effects on leukemia cells and cervical cancer cells, while Kap F-5-FL exhibited weaker staining effects. Further analysis of confocal images indicated the localization of Kap A-5-FL in the cells. This study reported the small-scale preparation and new delivery method of fluorescent probes for cell staining.
Article
Biochemistry & Molecular Biology
Matthias Roeber, Thomas Scheibel, Hans G. Boerner
Summary: Collagen mimetic peptides (CMPs) that imitate natural collagen are used in this study to construct a triple helix motif with DEPSI defects on an organic scaffold. The formation of the triple helix is monitored using circular dichroism spectroscopy. The presence of the DEPSI defects plays a crucial role in guiding the self-assembly and stability of the collagen motif.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ghazi O. Bou Ghanem, Dmitry Koktysh, Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, Lauren K. Wareham, David J. Calkins
Summary: This study investigates the potential of collagen mimetic peptides (CMPs) in repairing damaged collagen in ocular tissues. The results demonstrate that CMPs can restore the stiffness and structure of collagen in optic nerve head tissues, offering a promising therapeutic avenue for ocular disorders involving collagen remodeling and degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Alan B. Moran, Richard A. Gardner, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Daniel I. R. Spencer
Summary: Sialic acids play diverse biological roles and linkage-specific analysis is important in glycomic studies. The combination of procainamide fluorescent labeling with sialic acid linkage-specific derivatization is presented, which allows for unambiguous assignment of sialic acid linkages and separation of sialylated isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Neurosciences
Lauren K. K. Wareham, Joseph M. M. Holden, Olivia L. L. Bossardet, Robert O. O. Baratta, Brian J. J. Del Buono, Eric Schlumpf, David J. J. Calkins
Summary: The intraepithelial sub-basal nerve plexus of the cornea is crucial for maintaining ocular surface homeostasis. This study investigated the efficacy of topical application of a collagen mimetic peptide (CMP) in repairing the corneal nerve plexus in a mouse model of ocular surface desiccation. The results showed that CMP treatment significantly reduced nerve damage and improved corneal integrity.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Chemistry, Analytical
Hao Yu, Qiang Zhao
Summary: We report an aptamer microscale thermophoresis (MST) assay for the detection of Ochratoxin A (OTA), which offers the advantages of ratiometric analysis, rapid measurement, simple operation, high sensitivity, low sample consumption, and high throughput.
Article
Biochemistry & Molecular Biology
Marcio Ribeiro, Nolan R. McGrady, Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, David J. Calkins
Summary: Degeneration of retinal ganglion cell (RGC) axons can cause vision loss. We found that using collagen mimetic peptides (CMPs) can repair fragmented collagen and protect, repair, and regenerate RGC axons. Our experiments showed that intraocular injection of CMP can significantly reduce early RGC axon dysfunction and promote axonal recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Guanlie Li, Haiqing He, Guodong Zheng, Wenjing Jiang, Shuwen Du, Hua Tao, Tao Xiao, Dazhi Zhou, Shangwei Ding, Xi-Yong Yu, Yu Zhang, Ao Shen
Summary: This study developed an imaging modality to directly detect pulmonary fibrosis (PF) using a novel PF diagnostic agent called PVD (platelets-derived nanovesicles labeled with dye). The results showed that PVD could bind collagen and detect PF directly, making it a versatile probe for the direct visualization of collagen and potentially useful for clinical applications.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Materials Science, Multidisciplinary
Mengxi Guo, Qingli Zhou, Zheng Wang, Quanli Li, Chris Ying Cao
Summary: This study proposes a novel strategy of biomimetic collagen mineralization using ALONs to modify and cross-link collagen fibrils, promoting intrafibrillar collagen mineralization. The formation of ALONs-CaP complex increases the pool of mineralization precursors and enables the production of higher-quality mineralized collagen with better biomechanical properties in a short period of time.
MATERIALS TODAY ADVANCES
(2022)
Article
Biophysics
Fei Wang, Yichen Gong, Yang Xu, Zhanfang Ma, Hongliang Han
Summary: We constructed an electrochemical sensing interface using Ni2+ ions to guide the self-assembly of histidine-tagged peptides on the electrode for the detection of prostate specific antigen (PSA). The surface of the electrode was functionalized with carboxylated multiwalled carbon nanotubes and modified with N-(5-Amino-1-carboxypentyl) iminodiacetic acid (NIA) to capture Ni2+. The designed immune-functional peptide was then assembled onto the electrode surface, allowing for sensitive detection through the release of ferrocene upon protease recognition and cleavage. The self-assembly layer could be removed by pickling, enabling the reconstruction of the recognition layer.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Biomedical
Zhihua Li, Zijian Chen, Yanan Gao, Yi Xing, Yuping Zhou, Yucheng Luo, Weihong Xu, Zhengchang Chen, Xu Gao, Kapish Gupta, Karthic Anbalakan, Lei Chen, Chuang Liu, Jian Kong, Hwa Liang Leo, Chengzhi Hu, Hanry Yu, Qiongyu Guo
Summary: A new type of magnetic micro-anchor, SM(2)A, has been developed for magnetic guided endovascular localization. It demonstrates stable anchoring in the desired site and produces the necessary action. This micro-anchor can be a promising platform for precision treatment and diagnosis.
Article
Respiratory System
Shengren Song, Zhenli Fu, Ruijuan Guan, Jie Zhao, Penghui Yang, Yang Li, Hang Yin, Yunxin Lai, Gencheng Gong, Simin Zhao, Jiangtian Yu, Xiaomin Peng, Ying He, Yumei Luo, Nanshan Zhong, Jin Su
Summary: This study investigated the dynamic features of bleomycin-treated mouse lungs with fibrosis using a combination of the latest technologies and traditional methods. The results showed that while the total lung hydroxyproline contents reached a peak at 4 weeks after bleomycin injury, other fibrosis evaluation parameters indicated a gradual degradation of collagens and a recovery of lung aeration. The data from immunostaining and fluorescent peptide staining explained this inconsistency, showing that hydroxyproline-rich degraded collagen fragments were detained and enriched within the intracellular regions at 10 or 16 weeks after bleomycin treatment.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Engineering, Biomedical
Yucheng Luo, Yutao Ma, Zijian Chen, Yanan Gao, Yuping Zhou, Xiaoya Liu, Xuezhe Liu, Xu Gao, Zhihua Li, Chuang Liu, Hwa Liang Leo, Hanry Yu, Qiongyu Guo
Summary: Particulate embolic agents with calibrated sizes have gained significant interest in therapeutic embolotherapies. In this study, shape-anisotropic microembolics based on PVA were developed using a simple fabrication method. The embolization behaviors of these nonspherical microembolics were evaluated in vitro and in vivo models. The results demonstrated that compared to microspheres, the elongated microembolics displayed superior endovascular navigation capability, penetration depth, and embolization stability.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Yi-An Yang, Emmanuelle Nguyen, Gautham Hari Narayana Sankara Narayana, Melina Heuze, Chaoyu Fu, Hanry Yu, Rene-Marc Mege, Benoit Ladoux, Michael P. Sheetz
Summary: E-cadherin is a key molecule involved in mechanotransduction between cells in tissues. Epithelial cells are able to test and respond to the mechanical properties of neighboring cells through contractions of E-cadherin adhesions. This process requires the involvement of multiple proteins.
Article
Chemistry, Analytical
Xiaolin Wu, Cheryl Chan, Stacy L. Springs, Yie Hou Lee, Timothy K. Lu, Hanry Yu
Summary: WS-RADICA is a new nucleic acid detection method that enables rapid, sensitive, and quantitative detection of nucleic acids. It has a lower detection limit and higher inhibitor tolerance, and has been validated for nucleic acids derived from live viruses.
ANALYTICA CHIMICA ACTA
(2022)
Article
Physics, Applied
Yan Kong, Shujian Ong, Mei Hui Liu, Hanry Yu, Dejian Huang
Summary: This study successfully fabricated edible microbeads using food-grade sodium alginate and enhanced their cytoaffinity with gelatin coatings. The modified microbeads have self-fluorescence and can be used as microcarriers for cell culture and meat production.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Food Science & Technology
Sean Chia, Gavin Teo, Shi Jie Tay, Larry Sai Weng Loo, Corrine Wan, Lyn Chiin Sim, Hanry Yu, Ian Walsh, Kuin Tian Pang
Summary: This study utilized a glycomic approach to profile meat from different species, revealing distinct glycan profiles and significant differences in glycosylation attributes. By analyzing multi-attribute data, meat from different species could be clearly separated, demonstrating the potential of glycomics-based workflows for product authentication in various industries.
Article
Engineering, Environmental
Tao Yang, Dong Li, Yuhua Yan, Fatima-ezzahra Ettoumi, Ricardo A. Wu, Zisheng Luo, Hanry Yu, Xingyu Lin
Summary: In this study, a hydrogel-mediated RT-LAMP method was developed for rapid and accurate quantification of SARS-CoV-2 virus. The technique offers fast, simple, and highly sensitive detection, and can be used for monitoring cold chain foods.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yanan Gao, Jingyu Xiao, Zijian Chen, Yutao Ma, Xiaoya Liu, Dishuang Yang, Hwa Liang Leo, Hanry Yu, Jian Kong, Qiongyu Guo
Summary: Developing a three-dimensional in vitro tumor model with vasculature systems for testing endovascular interventional therapies is challenging. Here, an orthotopic liver tumor spheroid model is developed to evaluate transcatheter arterial chemoembolization (TACE) treatment. The model resembles the unique tumor microenvironment and can efficiently assess various diagnostic and therapeutic strategies.
BIOMATERIALS SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Qi Zhang, Xiaojing Li, Kui Huang, Yongjie Huang, Suwen Zhao, Shanshan Liu, Yang Li
Summary: Collagen hybridizing peptides (CHPs) have the ability to target collagen damage in pathological tissues. However, their tendency to self-trimerize hinders their applications. This study provides a solvent-switch method to control the self-assembly of CHP monomers, enabling their utilization in various applications.
Article
Chemistry, Multidisciplinary
Xiaojing Li, Qi Zhang, S. Michael Yu, Yang Li
Summary: Collagen provides support for human tissues and its damage can now be assessed through collagen hybridization. This research describes the development of collagen hybridization and the underlying rules of collagen triple-helix folding. It also discusses the biomedical evidence of collagen denaturation as an ECM signature for tissue remodeling and injuries, and proposes new questions and opportunities for diagnostic and therapeutic advancements.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Cell & Tissue Engineering
Kuin Tian Pang, Larry Sai Weng Loo, Sean Chia, Francesca Yi Teng Ong, Hanry Yu, Ian Walsh
Summary: Stem cells have the unique ability to differentiate into specialized cell types, and skeletal muscle stem cells (MuSCs) play important roles in the growth, repair, and regeneration of skeletal muscle tissues. However, the differentiation, proliferation, and expansion processes of MuSCs face significant challenges, and the molecular role of mechanobiology in MuSC growth, proliferation, and differentiation is poorly understood. This review comprehensively summarizes the impact of different mechanical cues on stem cell growth, proliferation, differentiation, and their potential role in disease development.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Materials Science, Biomaterials
Jie Zhao, Wenjun Yu, Qi Zhang, Xiaojing Li, Yongjie Huang, Suwen Zhao, Tao Li, Shanshan Liu, Yang Li, Hong Shan
Summary: Extracellular matrices decellularized from marine animal tissues, especially jellyfish, are promising scaffolds for tissue engineering. The decellularized jellyfish matrices showed structural similarity to mammalian collagen and supported cell adhesion and proliferation in 3D culture. In vivo experiments also demonstrated the potential of the decellularized jellyfish matrix for wound healing, making it a valuable scaffold for regenerative medicine.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Yingying Qi, Daoning Zhou, Julian L. Kessler, Rongmao Qiu, S. Michael Yu, Gang Li, Zhao Qin, Yang Li
Summary: Collagen, as the most abundant human repeat protein, features a hallmark triple-helix structure influenced by the terminal repeats. Studies have shown that collagen-mimetic peptides with different terminal repeats may exhibit distinct end structures and stabilities.
Article
Medicine, General & Internal
Yuan-Yang Tan, Hui-Qin Zhou, Yu-Jing Lin, Liu-Tong Yi, Zhuang-Gui Chen, Qing-Dong Cao, Yan-Rong Guo, Zhao-Ni Wang, Shou-Deng Chen, Yang Li, De-Yun Wang, Yong-Kang Qiao, Yan Yan
Summary: This study suggests that FGF2 is a potential inflammatory modulator in asthma, which is induced by house dust mites and acts through the FGFR/MAPK/NF-kappa B pathway in airway epithelial cells. The findings show that FGF2 expression is upregulated in asthmatic patients and is associated with inflammatory cell infiltration. Experimental results demonstrate that FGF2 can exacerbate airway inflammatory cell infiltration and exert its effects through the FGFR/MAPK/NF-kappa B pathway.
MILITARY MEDICAL RESEARCH
(2022)