Article
Chemistry, Analytical
Chang Liu, Xiaojie Jiao, Songtao Cai, Song He, Liancheng Zhao, Xianshun Zeng
Summary: Endogenous reactive oxygen species and reductive sulphur species play crucial roles in biological functions, influencing calcium homeostasis in neurons. A novel fluorescent probe PzSe with a reversible selenium atom can detect the redox cycle of HOCl/H2S with high sensitivity and selectivity. Through three-color fluorescence changes in PC-12 cells co-stained with PzSe and Ca2+-selective Fluo-4 AM, dynamic correlations among HOCl, H2S, and Ca2+ in neurons can be studied.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Timothy Kench, Viktoria Rakers, David Bouzada, Jacobo Gomez-Gonzalez, Jenna Robinson, Marina K. Kuimova, Miguel Vazquez Lopez, M. Eugenio Vazquez, Ramon Vilar
Summary: G-Quadruplex DNA structures have gained attention for their biological roles and potential in drug development. Certain sequences in the human telomere can form multiple adjacent G-quadruplexes. This study reports on the modular synthesis of dimeric metal-salphen complexes bridged by polyether or peptide linkers. The complexes show higher selectivity for dimeric G-quadruplexes and their emissive properties are used to study their interactions with G-quadruplexes in vitro and in cells.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Chemistry, Analytical
Haixian Ren, Fangjun Huo, Tianruo Shen, Xiaogang Liu, Caixia Yin
Summary: In this study, the reactivity of GSH was adjusted by combining HBT with different compounds, resulting in a rapid reversible ratiometric response to GSH. The probe was successfully used to monitor fluctuations in GSH concentration during cells apoptosis in real-time.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Yeonkyung Park, Junhyeok Yoon, Jinhwan Lee, Seoyoung Lee, Hyun Gyu Park
Summary: We have developed a new multicolor fluorogenic RNA aptasensor for the multiplexed detection of miRNAs. This aptasensor utilizes specific primers and enzymes to detect target miRNAs with high specificity, producing intense fluorescence signals that reflect the quantity of target miRNAs.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Biying Zhao, Ping Miao, Ziheng Hu, Xinying Zhang, Xue Geng, Yingying Chen, Lingyan Feng
Summary: This study reported a method for sensitive and specific detection of PSA through amperometric electrochemical measurements, with certain DNA aptamers showing higher signal variation when induced by PSA. With an optimized capture strand, the aptasensor exhibited good detection performance over a wide concentration range.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Guojun Wei, Zhibing Peng, Jingsong Liu, Kun Yang, Chenglong Zhao, Wei Xie, Tianwen Huang, Jiafeng Liu, Jin Li, Gang An
Summary: A novel CRISPR-Cas12a-based fluorescent sensing platform was proposed for sensitive and reliable analysis of telomerase activity. This platform can distinguish both the amounts of telomerase and the length of telomerase extension products. The introduction of the average telomerase activity index shows potential for accurate cancer reporting and osteosarcoma staging.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Oncology
Beatrix R. Bradford, Chunyuan Jin
Summary: Pre-mRNA processing of replication-dependent canonical histone mRNAs involves an endonucleolytic cleavage after a conserved stem loop structure before RNA Pol II encounters poly (A) signal. Unlike other eukaryotic mRNAs, canonical histone mRNAs are not polyadenylated at their 3' ends. The binding of SLBP to the stem loop structure is crucial for this process, and SLBP plays a role in regulation of histone mRNA nuclear export, degradation, and translation.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Kejun Hu, Xinyu Zhang, Pingfan Chen, Renju Lin, Jinlong Zhu, Zhen Huang, Haifeng Du, Dongsheng Song, Binghui Ge
Summary: The reversible phase transition between perovskite and oxygen-vacancy-ordered brownmillerite structures is observed in strained La0.7Sr0.3MnOx films under ultra-high vacuum conditions, and this abnormal behavior is found to be strain-dependent. The density functional theory calculations suggest that the high oxygen affinity of La0.7Sr0.3MnO2.5 is responsible for the reversible transitions.
MATERIALS TODAY PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Seth Scott, You Li, Oya Bermek, Jack D. Griffith, Stanley M. Lemon, Kyung H. Choi
Summary: Hepatitis C virus replication relies on the interaction between cellular factors miR-122 and PCBP2. These factors compete for binding to specific sites on the RNA genome, regulating the viral replication cycle. MiR-122 disrupts genome circularization and promotes recruitment of the viral polymerase by competing with PCBP2 for binding.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Alexei A. Sharov, Yuhki Nakatake, Weidong Wang
Summary: This study combined TF binding data with data on gene expression change after manipulation of individual TFs to develop an atlas of regulated targets of TFs (ART-TF) in human ES cells. This approach contributes to the understanding of biological functions of TFs and organization of gene regulatory networks. This atlas should be a valuable resource for ES cell-based regenerative medicine studies.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Raja Rajakani, Pravin Prakash, Dolly Ghosliya, Ranjana Soni, Arpita Singh, Vikrant Gupta
Summary: This study reports the extensive identification of miRNAs and their possible targets in Azadirachta indica for the first time, indicating the therapeutic potential of this medicinal tree. The miRNAs identified in neem range in size between 19 and 23 nt, with a potential negative correlation between miRNA expression and their targets.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Mandar Bopardikar, Sri Rama Koti Ainavarapu, Ramakrishna V. Hosur
Summary: Experimental evidence shows that compounds containing different numbers of catechol rings, such as Pyrogallol, Corilagin, and Chebulagic acid, can independently delay and reduce the extent of alpha-Synuclein fibrillization. These compounds interact with the N-terminal region of the protein, inhibiting its fibrillization.
Article
Chemistry, Multidisciplinary
Daixi Liu, Haibo Lv, Yafei Wang, Jinyu Chen, Dexin Li, Ranran Huang
Summary: This study presents the first genome-wide targetome analysis of enzymes involved in selective RNA processing and stabilization (SRPS) in Escherichia coli. The researchers discovered a linear relationship between the RNA pyrophosphohydrolase processing ratio and scores assigned to the first three nucleotides of the primary transcript. They also found that the folding free energy of stem-loops associated with PNPase targetomes is negatively correlated with the termination ratio of PNPase at the 3' end. Moreover, the study revealed that certain stem-loop structures formed by RNase E processing sites in the 5'-untranslated regions (UTR) affect ribosome-binding and translation efficiency. The effectiveness of SRPS elements was validated using a dual-fluorescence reporter system. These findings demonstrate a multi-layer and quantitative regulatory method for optimizing the stoichiometric expression of genes in bacteria, with potential applications in synthetic biology.
Review
Immunology
Shizuo Akira, Kazuhiko Maeda
Summary: RBPs play a crucial role in regulating mRNA turnover and translation by binding cis-regulatory elements in mRNA's 3' UTRs. They can collaboratively or competitively bind the same target mRNA to enhance or dampen regulatory activities. Dysregulation of RBPs can lead to chronic inflammation and autoimmunity.
ANNUAL REVIEW OF IMMUNOLOGY, VOL 39
(2021)
Article
Food Science & Technology
JunGang Du, Biao Ma, Jiali Li, Jiangbing Shuai, Xiaoping Yu, Xiaofeng Zhang, Mingzhou Zhang
Summary: The probe-based loop-mediated isothermal amplification is an efficient tool for the simultaneous detection of multiple bacterial pathogens in a short period of time. This method can identify three bacterial target fragments within 25 minutes and can be used for the detection of contaminated foods after enrichment.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Medicinal
Dean Y. Maeda, Angela M. Peck, Aaron D. Schuler, Mark T. Quinn, Liliya N. Kirpotina, Winston N. Wicomb, Richard L. Auten, Rambabu Gundla, John A. Zebala
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2015)
Article
Chemistry, Medicinal
Aaron D. Schuler, Courtney A. Engles, Dean Y. Maeda, Mark T. Quinn, Liliya N. Kirpotina, Winston N. Wicomb, S. Nicholas Mason, Richard L. Auten, John A. Zebala
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2015)
Article
Multidisciplinary Sciences
Xin Lu, James W. Horner, Erin Paul, Xiaoying Shang, Patricia Troncoso, Pingna Deng, Shan Jiang, Qing Chang, Denise J. Spring, Padmanee Sharma, John A. Zebala, Dean Y. Maeda, Y. Alan Wang, Ronald A. DePinho
Article
Chemistry, Medicinal
Dean Y. Maeda, Angela M. Peck, Aaron D. Schuler, Mark T. Quinn, Liliya N. Kirpotina, Winston N. Wicomb, Guo-Huang Fan, John A. Zebala
JOURNAL OF MEDICINAL CHEMISTRY
(2014)
Article
Multidisciplinary Sciences
John A. Zebala, Alan Mundell, Linda Messinger, Craig E. Griffin, Aaron D. Schuler, Stuart J. Kahn
Article
Oncology
Wenting Liao, Michael J. Overman, Adam T. Boutin, Xiaoying Shang, Di Zhao, Prasenjit Dey, Jiexi Li, Guocan Wang, Zhengdao Lan, Jun Li, Ming Tang, Shan Jiang, Xingdi Ma, Peiwen Chen, Riham Katkhuda, Krittiya Korphaisarn, Deepavali Chakravarti, Andrew Chang, Denise J. Spring, Qing Chang, Jianhua Zhang, Dipen M. Maru, Dean Y. Maeda, John A. Zebala, Scott Kopetz, Y. Alan Wang, Ronald A. DePinho
Article
Clinical Neurology
John A. Zebala, Shawn L. Searle, Lynn R. Webster, Matt S. Johnson, Aaron D. Schuler, Dean Y. Maeda, Stuart J. Kahn
Article
Pharmacology & Pharmacy
John A. Zebala, Aaron D. Schuler, Stuart J. Kahn, Dean Y. Maeda
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Oncology
Lucas A. Horn, Kristen Fousek, Duane H. Hamilton, James W. Hodge, John A. Zebala, Dean Y. Maeda, Jeffrey Schlom, Claudia Palena
Summary: Innovative strategies involving the addition of cancer vaccines to immune checkpoint blockade therapies can enhance antitumor efficacy by increasing the numbers of tumor-infiltrating T cells. These strategies have shown promising results in preclinical studies and may have potential in improving outcomes for patients resistant to current immunotherapies.
Article
Multidisciplinary Sciences
Vidya C. Sinha, Amanda L. Rinkenbaugh, Mingchu Xu, Xinhui Zhou, Xiaomei Zhang, Sabrina Jeter-Jones, Jiansu Shao, Yuan Qi, John A. Zebala, Dean Y. Maeda, Florencia McAllister, Helen Piwnica-Worms
Summary: The aggressive and indolent breast tumor cells are functionally indistinguishable once isolated from their local niche, suggesting the involvement of non-tumor collaborators in determining aggressiveness. Aggressive lesions are characterized by fewer total but more suppressed-like T cells, elevated tumor-promoting neutrophils, and IL-17 signaling, disruption of which can increase tumor latency and reduce the number of aggressive lesions.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Kwan Ho Tang, Shuai Li, Alireza Khodadadi-Jamayran, Jayu Jen, Han Han, Kayla Guidry, Ting Chen, Yuan Hao, Carmine Fedele, John A. Zebala, Dean Y. Maeda, James G. Christensen, Peter Olson, Argus Athanas, Cynthia A. Loomis, Aristotelis Tsirigos, Kwok-Kin Wong, Benjamin G. Neel
Summary: Our study suggests that inhibiting the SHP2/RAS/ERK pathway leads to upregulation of CXCR2 ligands and recruitment of S100A8 hi gMDSCs, suppressing T cells in non-small cell lung cancer. Combining SHP2/CXCR2 inhibitors can block gMDSC immigration, enhance Th1 polarization, induce highly cytotoxic CD8 + KLRG1 + effector T cells, and improve survival.
Article
Oncology
Pat Gulhati, Aislyn Schalck, Shan Jiang, Xiaoying Shang, Chang-Jiun Wu, Pingping Hou, Sharia Hernandez Ruiz, Luisa Solis Soto, Edwin Parra, Haoqiang Ying, Jincheng Han, Prasenjit Dey, Jun Li, Pingna Deng, Emi Sei, Dean Y. Maeda, John A. Zebala, Denise J. Spring, Michael Kim, Huamin Wang, Anirban Maitra, Dirk Moore, Karen Clise-Dwyer, Y. Alan Wang, Nicholas E. Navin, Ronald A. DePinho
Summary: Pancreatic ductal adenocarcinoma (PDAC) is resistant to PD1 and CTLA4 immune checkpoint therapies. This study characterized the mechanisms of ICT resistance and identified effective therapeutic options. The combination of agonist 41BB and antagonist LAG3 ICT increased survival and antitumor immunity. Triple therapy with T cell-activating ICTs and a CXCR1/2 inhibitor resulted in durable complete responses. This provides a testable hypothesis for clinical testing in human PDAC.
Article
Medicine, Research & Experimental
Julia Kargl, Xiaodong Zhu, Huajia Zhang, Grace H. Y. Yang, Travis J. Friesen, Melissa Shipley, Dean Y. Maeda, John A. Zebala, Jill McKay-Fleisch, Gavin Meredith, Afshin Mashadi-Hossein, Christina Baik, Robert H. Pierce, Mary W. Redman, Jeffrey C. Thompson, Steven M. Albelda, Hamid Bolouri, A. McGarry Houghton
Article
Medicine, Research & Experimental
Lillian Sun, Paul E. Clavijo, Yvette Robbins, Priya Patel, Jay Friedman, Sarah Greene, Rita Das, Chris Silvin, Carter Van Waes, Lucas A. Horn, Jeffrey Schlom, Claudia Palena, Dean Maeda, John Zebala, Clint T. Allen
Article
Pharmacology & Pharmacy
Alan Menter, Breck Thrash, Christina Cherian, Larry H. Matherly, Lei Wang, Aleem Gangjee, Joel R. Morgan, Dean Y. Maeda, Aaron D. Schuler, Stuart J. Kahn, John A. Zebala
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2012)