期刊
BIOCONJUGATE CHEMISTRY
卷 22, 期 6, 页码 1006-1011出版社
AMER CHEMICAL SOC
DOI: 10.1021/bc100593m
关键词
-
类别
资金
- National Institutes of Health [CA-16042, AI-28697]
- Johsson Cancer Center
- UCLA AIDS Institute
- David Geffen School of Medicine at UCLA
- NIH/NIBIB BRP [R01-EB000312]
- NSF
- Center for Biophotonics, an NSF Science and Technology Center [PHY0120999]
- Marie Curie-Intra-European Fellowship [MEIF-CT-2006-040210]
- EMBO
- Centre de Nanoscience Be de France
- Fondation pour la Recherche Medicale
We present a robust scheme for preparation of semiconductor quantum dots (QDs) and cognate partners in a conjugation ready format. Our approach is based on bis-aryl hydrazone bond formation mediated by aromatic aldehyde and hydrazinonicotinate acetone hydrazone (HyNic) activated peptide coated quantum dots. We demonstrate controlled preparation of antibody-QD bioconjugates for specific targeting of endogenous epidermal growth factor receptors in breast cancer cells and for single QD tracking of transmembrane proteins via an extracellular epitope. The same approach was also used for optical mapping of RNA polymerases bound to combed genomic DNA in vitro.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据