Article
Chemistry, Organic
Jan-Erik Hornung, Timo Weinrich, Michael W. Goebel
Summary: Quinone methide precursors protected with alkyldithiomethyl groups have been synthesized and converted into PNA conjugates. These conjugates remain stable without reducing agents, but release electrophilic quinone methide in the presence of glutathione, typical of cytosolic concentrations. Self-alkylation or crosslinking of RNA occurs when hybridized with complementary strands, with the fastest reactions observed for the least hindered compound.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Nibir Pathak, Cesar A. Patino, Namrata Ramani, Prithvijit Mukherjee, Devleena Samanta, Sasha B. Ebrahimi, Chad A. Mirkin, Horacio D. Espinosa
Summary: We demonstrate the efficient intracellular delivery of large proteins, protein-nucleic acid conjugates, and Cas9-ribonucleoprotein complex using a nanochannel-based localized electroporation platform. This platform also allows for the improved cytosolic delivery of spherical nucleic acids, expanding opportunities for detection and therapy.
Article
Biotechnology & Applied Microbiology
Jie Dai, Jun Wang, Xuan Yang, Zixing Xu, Gang Ruan
Summary: Understanding the transport mechanism of biological delivery is crucial for developing delivery technologies. In this study, the cellular transport mechanism of SDots-Tat was investigated using live-cell spinning-disk confocal microscopy and molecular biology methods. The findings revealed a new pathway for SDots-Tat to enter the cell nucleus, offering a new approach to overcome the nucleus membrane barrier in designing biological delivery technologies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Andrea Mosseri, Maria Sancho-Albero, Marilisa Leone, Donatella Nava, Francesco Secundo, Daniela Maggioni, Luisa De Cola, Alessandra Romanelli
Summary: This study reports the formation of homogeneous chiral fibers through self-assembly of peptide nucleic acids. Nucleobases play a key role in determining the morphology and chirality of the fibers. By spectroscopic characterization and microscopy studies, a structural model consistent with the experimental evidence was obtained.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Tetsuya Nagata, Chrissa A. Dwyer, Kie Yoshida-Tanaka, Kensuke Ihara, Masaki Ohyagi, Hidetoshi Kaburagi, Haruka Miyata, Satoe Ebihara, Kotaro Yoshioka, Takashi Ishii, Kanjiro Miyata, Kenichi Miyata, Berit Powers, Tomoko Igari, Syunsuke Yamamoto, Naoto Arimura, Hideki Hirabayashi, Toshiki Uchihara, Rintaro Iwata Hara, Takeshi Wada, C. Frank Bennett, Punit P. Seth, Frank Rigo, Takanori Yokota
Summary: This study demonstrates that DNA/RNA heteroduplex oligonucleotides (HDOs) conjugated to cholesterol or alpha-tocopherol can regulate endogenous gene expression in the central nervous system when administered systemically, with limited side effects. The HDOs effectively distribute throughout the CNS and suppress the expression of target genes by up to 90%, showing potential for ASO-based therapies for neurological diseases.
NATURE BIOTECHNOLOGY
(2021)
Article
Polymer Science
Zhaofan Yang, Shijie Liang, Baiqiao Liu, Jing Wang, Fan Yang, Qiaomei Chen, Chengyi Xiao, Zheng Tang, Weiwei Li
Summary: A new semi-flexible linker was developed for single-component organic solar cells, but the decreased charge transporting properties resulted in lower power conversion efficiencies for devices based on the new polymers.
Article
Chemistry, Multidisciplinary
Dmytro Honcharenko, Kristina Druceikaite, Malgorzata Honcharenko, Martin Bollmark, Ulf Tedebark, Roger Stromberg
Summary: The report describes a simplified methodology for multiple labeling of oligonucleotides on a solid support, utilizing novel linkers with orthogonal functionalities to allow for attachment of varied moieties. The versatility of the approach is demonstrated through synthesis of oligonucleotide multiconjugates with different functional entities.
Article
Chemistry, Organic
Ashwani Sharma, Shahaji H. More, Krishna N. Ganesh
Summary: The study introduces a novel cationic PNA, dapPNA, which demonstrates that the electrostatic attraction between cationic dapPNA and negatively charged DNA can enhance the stability of PNA : DNA complexes, overcoming unfavorable stereochemical effects. Additionally, the synthesis of two different PNA backbones derived from a single PNA monomer expands the repertoire of pyrrolidine-based PNA analogues.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
M. Biedulska, P. Jakobczyk, M. Sosnowska, B. Dec, A. Muchlinska, A. J. Zaczek, D. Nidzworski, R. Bogdanowicz
Summary: A novel procedure for the stabilization and functionalization of FLBP involving PLL and PEG has been proposed in this study, resulting in improved environmental stability and reduced cytotoxicity against HB2 cells. The results suggest a promising future application of black phosphorus nanosheets as nanocarriers, with potential for enhanced therapeutic effects.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Marika Comegna, Gemma Conte, Andrea Patrizia Falanga, Maria Marzano, Gustavo Cernera, Antonella Miriam Di Lullo, Felice Amato, Nicola Borbone, Stefano D'Errico, Francesca Ungaro, Ivana d'Angelo, Giorgia Oliviero, Giuseppe Castaldo
Summary: Cystic fibrosis is characterized by airway obstruction caused by malfunctioning CFTR protein, and the main challenge in treatment is enhancing drug delivery to the lungs. This study reports the production and characterization of hybrid core-shell nanoparticles loaded with PNA for potential therapeutic use, showing their ability to overcome mucus barrier and release cargo in the cytoplasm.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Haimei Cao, Meijun Yi, Henan Wei, Shiping Zhang
Summary: Smart multifunctional polymeric micelles loaded with folate and pH-responsive cell membrane mimetic were successfully prepared, and the micelles exhibited excellent drug release properties and anti-tumor activity. These micelles have the potential to be applied in tumor therapy.
Review
Biochemistry & Molecular Biology
Sayedeh Azimeh Hosseini, Arefeh Kardani, Hajar Yaghoobi
Summary: Nucleic acids provide a promising therapeutic platform by targeting various cell signaling pathways involved in cancer and genetic disorders, but maintaining optimal stability during delivery limits their utility. Gold nanoparticle-nucleic acid combinations have been shown to be highly efficient mineral vectors for overcoming these obstacles.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Sinan Faiad, Quentin Laurent, Alexander L. Prinzen, Jathavan Asohan, Daniel Saliba, Violeta Toader, Hanadi F. Sleiman
Summary: Nucleic acid therapeutics (NATs) are efficient tools for gene expression modulation and treating undruggable diseases. Spherical nucleic acids (SNAs) can effectively deliver small NATs to cells and protect them from degradation while improving biodistribution and reducing immune activation. Self-assembled SNAs, nanostructures made from a single DNA-polymer conjugate, have similar properties as small molecule encapsulation. A study on the factors that govern the fate of self-assembled SNAs reveals the importance of extracellular stability for recognition by membrane receptors and cellular uptake, as well as the requirement for intracellular dissociation for efficient therapeutic release. Disulfide-crosslinked SNAs combine these two properties and result in efficient and non-toxic gene silencing therapeutics. These findings will aid in the translation of self-assembled structures for in vivo gene silencing applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Christopher Liczner, Cameron C. Hanna, Richard J. Payne, Christopher J. Wilds
Summary: This study presents an efficient method for synthesizing peptide-oligonucleotide conjugates using a diselenide-selenoester ligation strategy, which leads to stable amide linkages between the two biomolecules. The novel conjugation strategy increases synthesis efficiency and expands the applications of chemical modification.
Article
Biochemistry & Molecular Biology
Yusuke Hamashita, Takahiro Shibata, Akiko Takeuchi, Takashi Okuno, Naoki Kise, Toshihiko Sakurai
Summary: The artificial nucleic acid i-PPc demonstrated the ability to detect single nucleotide mutations and suppress gene expression, showing potential for antisense therapy targeting single nucleotide polymorphisms (SNP).
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)