Synthesis and Biophysical Studies of Coronene Functionalized 2 '-Amino-LNA: A Novel Class of Fluorescent Nucleic Acids

Incorporation of 2'-N-(coronen-l-yl)methyl-2'-amino-LNA monomer X or 2'-N-4-(coronen-l-yl)-4-oxobutanoyl-2'-amino-LNA monomer Y into short DNA strands induces high binding affinity toward DNA or RNA and a marked res-shift in steady-state fluorescence emission upon hybridization to cDNA or RNA.