DNA interaction of europium(III) complex containing 2,2′-bipyridine and its antimicrobial activity

The interaction of native fish salmon DNA (FS-DNA) with [Eu(bpy)(3)Cl-2(H2O)]Cl, where bpy is 2,2 '-bipyridine, is studied at physiological pH in Tris-HCl buffer by spectroscopic methods, viscometric techniques as well as circular dichroism (CD). These experiments reveal that Eu(III) complex has interaction with FS-DNA. Moreover, binding constant and binding site size have been determined. The value of K-b has been defined 2.46 +/-.02x10(5)M(-1). The thermodynamic parameters are calculated by Van't Hoff equation, the results show that the interaction of the complex with FS-DNA is an entropically driven phenomenon. CD spectroscopy followed by viscosity as well as fluorescence and UV--Vis measurements indicate that the complex interacts with FS-DNA via groove binding mode. Also, the synthesized Eu(III) complex has been screened for antimicrobial activities.