期刊
BIOCHIMIE
卷 94, 期 2, 页码 574-577出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2011.07.030
关键词
DNA cross-linking; Diepoxybutane; DNA bending; DNA damage; Alkylating agent
资金
- American Chemical Society [44839-B4]
- NIH from the INBRE of the National Center for Research Resources [P20RR016463]
The bifunctional alkylating agent 1,2,3,4-diepoxybutane (DEB) is thought to be a major contributor to the carcinogenicity of 1,3-butadiene, from which it is derived in vivo. DEB forms DNA interstrand cross-links primarily between distal deoxyguanosine residues at the duplex sequence 5'-GNC. In order for the short butanediol tether to span this distance, distortion of the DNA target has been postulated. We determined that the electrophoretic mobility of ligated DNA oligomers containing DEB cross-links was retarded in comparison with control, uncross-linked DNA. Our data are consistent with DNA bending of 34 per lesion towards the major groove. (C) 2011 Elsevier Masson SAS. All rights reserved.
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