Article
Biochemistry & Molecular Biology
Caterina Bernacchioni, Veronica Ghini, Roberta Squecco, Eglantina Idrizaj, Rachele Garella, Elisa Puliti, Francesca Cencetti, Paola Bruni, Chiara Donati
Summary: Skeletal muscle atrophy is characterized by a decrease in muscle mass causing reduced agility, increased fatigability, and higher risk of bone fractures. Inflammatory cytokine TNF alpha is a strong inducer of skeletal muscle atrophy. The bioactive sphingolipid S1P plays an important role in skeletal muscle biology, exerting most of its actions through its specific receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Kana Masuda-Kuroki, Shahrzad Alimohammadi, Anna Di Nardo
Summary: Psoriasis is a chronic skin condition that lacks a complete cure. Recent studies have identified sphingolipid metabolites as significant contributors to psoriasis, particularly ceramide and sphingosine-1-phosphate (S1P). The modulation of S1P and its receptor has shown potential in improving psoriasis inflammation.
Article
Cell Biology
Ana Gomez-Larrauri, Alberto Ouro, Miguel Trueba, Antonio Gomez-Munoz
Summary: C1P is a bioactive sphingolipid that plays key roles in cellular functions and inflammation-associated pathologies. It promotes cell growth and survival through various signaling pathways, and has shown potential as an anti-inflammatory agent in lung inflammation.
CELLULAR SIGNALLING
(2021)
Review
Oncology
Antonia Piazzesi, Sumaiya Yasmeen Afsar, Gerhild Van Echten-Deckert
Summary: Cancer development is a complex process involving overcoming obstacles such as unrestricted proliferation, invasion, nutrient supply, and metastasis, while evading the immune system. Inflammation plays a role in cancer development, with sphingolipid metabolism impacting cells' progression from healthy to cancerous phenotypes.
MOLECULAR ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Peter Horvath, Lilla Budi, Daniel Hammer, Rita Varga, Gyoergy Losonczy, Adam Domonkos Tarnoki, David Laszlo Tarnoki, Martina Meszaros, Andras Bikov
Summary: Chronic inflammation induced by hypoxia during sleep is an important mechanism of microvascular damage in OSA patients. This study examined the role of the sphingosine rheostat, which has various inflammatory effects. The results showed elevated levels of ceramide antibody and sphingosine-1-phosphate in patients with OSA, suggesting their involvement in the pathomechanism and comorbidities of OSA.
SCIENTIFIC REPORTS
(2023)
Article
Obstetrics & Gynecology
Caterina Bernacchioni, Tommaso Capezzuoli, Valentina Vannuzzi, Francesca Malentacchi, Francesca Castiglione, Francesca Cencetti, Marcello Ceccaroni, Chiara Donati, Paola Bruni, Felice Petraglia
Summary: This study investigates the molecular mechanisms involved in the fibrotic trait of endometriosis through examining the alterations of the S1P signaling pathway. The results show a deep dysregulation of S1P signaling in endometriosis, with S1P synthesis enzymes and receptors playing a crucial role in promoting fibrosis.
FERTILITY AND STERILITY
(2021)
Review
Biochemistry & Molecular Biology
Yali Lai, Yue Tian, Xintong You, Jiangnan Du, Jianmei Huang
Summary: Many cardiovascular disorders are characterized by endothelial cell dysfunction, which is closely related to sphingolipid metabolism. Understanding the effects of sphingolipid metabolites and key enzymes on endothelial cells can provide insights into the pathogenesis of cardiovascular diseases and potential therapeutic targets.
LIPIDS IN HEALTH AND DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Rosaria Bassi, Stefania Brambilla, Cristina Tringali, Paola Giussani
Summary: The study found that GBM cells overexpressing EGFR have higher levels of S1P and increased SK1 activity, leading to resistance to the standard chemotherapeutic drug temozolomide. This suggests a functional link between EGFR and S1P signaling pathways in enhancing the survival properties of GBM cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Mateusz Matwiejuk, Hanna Mysliwiec, Adrian Chabowski, Iwona Flisiak
Summary: Psoriasis is a complex, chronic, immunologically mediated disease that is associated with numerous other diseases. It can cause impairment of quality of life and may be associated with depressive disorders. The pathophysiology of psoriasis and its comorbidities is not fully understood yet, and disrupted metabolism of sphingolipids may be the link between them.
Review
Cell Biology
Federica Cirillo, Marco Piccoli, Andrea Ghiroldi, Michelle M. Monasky, Paola Rota, Paolo La Rocca, Adriana Tarantino, Sara D'Imperio, Paola Signorelli, Carlo Pappone, Luigi Anastasia
Summary: Cardiovascular diseases are a major cause of death globally, with an increasing number of patients posing challenges to healthcare systems. Sphingolipids have emerged as important bioactive compounds that play crucial roles in biological processes and have potential therapeutic implications for managing cardiovascular diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Ophthalmology
M. Victoria Simon, Marcela S. Vera, Paula E. Tenconi, Tamara Soto, Facundo H. Prado Spalm, Camila Torlaschi, Melina Mateos, Nora P. Rotstein
Summary: Retinal pigment epithelium (RPE) cells play a crucial role in maintaining retina homeostasis but also contribute to the development of retina proliferative diseases. Recent studies have revealed that Sphingosine-1-phosphate (S1P) and ceramide-1-phosphate (C1P) can stimulate migration and inflammation in RPE cells, suggesting their potential as targets for treating these pathologies.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Miira M. M. Klemetti, Sruthi Alahari, Martin Post, Isabella Caniggia
Summary: Disturbances in lipid metabolism are common in diabetes. This study compared the placental sphingolipid metabolism in type 1 and type 2 diabetic pregnancies and non-diabetic controls. The results showed that placental ceramide levels were lower in diabetic pregnancies, but higher in cases of preeclampsia and poor glycemic control. Sphingosine kinases (SPHK) and sphingoid-1-phosphates (S1P) were elevated in diabetic pregnancies, especially in cases of fetal macrosomia.
Article
Biochemistry & Molecular Biology
Mateusz Matwiejuk, Hanna Mysliwiec, Bartlomiej Lukaszuk, Marta Lewoc, Hend Malla, Piotr Mysliwiec, Jacek Dadan, Adrian Chabowski, Iwona Flisiak
Summary: Psoriasis is a complex chronic immunologically mediated disease that affects the skin, nails, and joints. Abnormal levels of sphingolipids significantly differ between psoriatic skin and healthy skin, suggesting their potential role in the pathogenesis of psoriasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Urology & Nephrology
Yelena Drexler, Judith Molina, Alla Mitrofanova, Alessia Fornoni, Sandra Merscher
Summary: Recent studies have shown that sphingolipids and sphingolipid metabolites play important roles in the pathogenesis and progression of kidney diseases by influencing the intracellular sphingolipid composition of renal cells and the integrity of the glomerular filtration barrier. The sphingolipid metabolite S1P plays a crucial role in renal cells, and aberrant S1P signaling contributes to the pathogenesis and progression of kidney diseases.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Ophthalmology
Marcela S. Vera, M. Victoria Simon, Facundo H. Prado Spalm, Victoria B. Ayala-Pena, O. Lorena German, Luis E. Politi, Florencia X. Santiago Valtierra, Nora P. Rotstein
Summary: This study demonstrated that Ceramide-1-phosphate (C1P) can promote the migration of Muller glial cells by activating cPLA2, PI3K, and ERK/MAPK pathways. In addition, CerK-mediated C1P synthesis contributes to glial cell migration, suggesting a novel role of C1P in controlling glial motility.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Vinodh Rajagopalan, Daniel Canals, Chiara Luberto, Justin Snider, Christina Voelkel-Johnson, Lina M. Obeid, Yusuf A. Hannun
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2015)
Article
Biochemistry & Molecular Biology
K. Alexa Orr Gandy, Lina M. Obeid
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2013)
Review
Biochemistry & Molecular Biology
Mohamad Adada, Daniel Canals, Yusuf A. Hannun, Lina M. Obeid
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2014)
Review
Biochemistry & Molecular Biology
Jean-Philip Truman, Monica Garcia-Barros, Lina M. Obeid, Yusuf A. Hannun
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2014)
Article
Biochemistry & Molecular Biology
Mohamad El Osta, Mengling Liu, Mohamad Adada, Can E. Senkal, Jolanta Idkowiak-Baldys, Lina M. Obeid, Christopher J. Clarke, Yusuf A. Hannun
Article
Biochemistry & Molecular Biology
Mohamad M. Adada, K. Alexa Orr-Gandy, Ashley J. Snider, Daniel Canals, Yusuf A. Hannun, Lina M. Obeid, Christopher J. Clarke
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Jessica S. Ross, Wei Hu, Bess Rosen, Ashley J. Snider, Lina M. Obeid, L. Ashley Cowart
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
David M. Perry, Benjamin Newcomb, Mohamad Adada, Bill X. Wu, Patrick Roddy, Kazuyuki Kitatani, Leah Siskind, Lina M. Obeid, Yusuf A. Hannun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Kali Janes, Joshua W. Little, Chao Li, Leesa Bryant, Collin Chen, Zhoumou Chen, Krzysztof Kamocki, Timothy Doyle, Ashley Snider, Emanuela Esposito, Salvatore Cuzzocrea, Erhard Bieberich, Lina Obeid, Irina Petrache, Grant Nicol, William L. Neumann, Daniela Salvemini
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Wataru Sakamoto, Nicolas Coant, Daniel Canals, Lina M. Obeid, Yusuf A. Hannun
JOURNAL OF LIPID RESEARCH
(2018)
Article
Multidisciplinary Sciences
Ashley J. Snider, Wahida H. Ali, Jonathan A. Sticca, Nicolas Coant, Amr M. Ghaleb, Toshihiko Kawamori, Vincent W. Yang, Yusuf A. Hannun, Lina M. Obeid
Article
Biochemistry & Molecular Biology
Wataru Sakamoto, Daniel Canals, Silvia Salamone, Janet Allopenna, Christopher J. Clarke, Justin Snider, Lina M. Obeid, Yusuf A. Hannun
JOURNAL OF LIPID RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Mohamed F. Salama, Mengling Liu, Christopher J. Clarke, Mel Pilar Espaillat, John D. Haley, Ting Jin, Daifeng Wang, Lina M. Obeid, Yusuf A. Hannun
Article
Cell Biology
Magali Trayssac, Christopher J. Clarke, Jeffrey L. Stith, Justin M. Snider, Naomi Newen, Christopher R. Gault, Yusuf A. Hannun, Lina M. Obeid
Summary: Senescence is an antiproliferative mechanism that can suppress tumor development and be induced by oncogenes like genes of the Ras family. Targeting SK1 could potentially enhance oncogene-induced senescence by increasing VLC Cer downstream of CerS2.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Maria Jose Hernandez-Corbacho, Mohamed F. Salama, Daniel Canals, Can E. Senkal, Lina M. Obeid
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2017)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
