期刊
BIOCHIMIE
卷 90, 期 10, 页码 1427-1434出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2008.03.013
关键词
Turnip mosaic virus; Viral protein linked to the genome; Eukaryotic initiation factor; Translational initiation; In vitro translation
资金
- Ministry of Education, Science, Sports & Culture of Japan [18580045, 17208004]
- Grants-in-Aid for Scientific Research [18580045, 17208004] Funding Source: KAKEN
The interaction between turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) and Arabidopsis thaliana eukaryotic initiation factor (iso)4E (eIF(iso)4E) was investigated to address the influence of potyviral VPg on host cellular translational initiation. Affinity chromatographic analysis showed that the region comprising amino acids 62-70 of VPg is important for the interaction with eIF(iso)4E. In vitro translation analysis showed that the addition of VPg significantly inhibited translation of capped RNA in eIF(iso)4E-reconstituted wheat germ extract. This result indicates that VPg inhibits cap-dependent translational initiation via binding to eIF(iso)4E. The inhibition by VPg of in vitro translation of RNA with wheat germ extract did not depend on RNase activity. Our present results may indicate that excess VPg produced at the encapsidation stage shuts off cap-dependent translational initiation in host cells by inhibiting complex formation between eIF(iso)4E and cellular mRNAs. (C) 2008 Elsevier Masson SAS. All rights reserved.
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