Article
Cell Biology
Despoina Sakellariou, Sara Thornby Bak, Esin Isik, Sonia Barroso, Antonio Porro, Andres Aguilera, Jiri Bartek, Pavel Janscak, Javier Pena-Diaz
Summary: The study reveals that the mismatch repair protein MutS beta is enriched at telomeres in ALT cancer cells, preventing the accumulation of G-quadruplex (G4) structures and R-loops, thus maintaining telomere integrity during alternative lengthening of telomeres.
Review
Biochemistry & Molecular Biology
Eugene Shakirov, Julian J-L Chen, Dorothy E. Shippen
Summary: This review summarizes the recent advances in plant telomere biology and compares it to telomere and telomerase research in other eukaryotic lineages. Despite the divergence between plants and animals over a billion years ago, the knowledge gained from plants has continued to contribute to the understanding of telomere biology and its implications for human health, aging, and stress responses. Studies of plant telomeres have revealed unexpected divergence in telomere sequence and architecture, and have provided new insights into the evolution and core components of telomerase across major eukaryotic kingdoms. Additionally, the cataloging of natural variation in plants like Arabidopsis thaliana and maize has provided valuable information on the genetic networks governing telomere length and its interactions with environmental factors and plant physiology.
Article
Biochemistry & Molecular Biology
Chih-Yi Gabriela Lin, Anna Christina Naeger, Thomas Lunardi, Aleksandra Vancevska, Gerald Lossaint, Joachim Lingner
Summary: Telomere shortening can lead to diseases and aging, often due to the lack of telomerase in cells. By developing a new isolation protocol, specific proteins associated with telomere replication can be purified, revealing their importance for maintaining telomere stability. This study provides a valuable resource for understanding the dynamic changes of the telomeric proteome during replication.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Natthakan Thongon, Feiyang Ma, Andrea Santoni, Matteo Marchesini, Elena Fiorini, Ashley Rose, Vera Adema, Irene Ganan-Gomez, Emma M. Groarke, Fernanda Gutierrez-Rodrigues, Shuaitong Chen, Pamela Lockyer, Sarah Schneider, Carlos Bueso-Ramos, Guillermo Montalban-Bravo, Caleb A. Class, Kelly A. Soltysiak, Matteo Pellegrini, Ergun Sahin, Alison A. Bertuch, Courtney D. DiNardo, Guillermo Garcia-Manero, Neal S. Young, Karen Dwyer, Simona Colla
Summary: The study reveals that telomere attrition leads hematopoietic stem cells towards differentiation into megakaryocytic lineage through upregulated innate immune signaling response, ultimately compromising their self-renewal capabilities and leading to depletion. Targeting the IFI16 signaling axis could potentially prevent functional decline of hematopoietic stem cells under conditions affecting telomere maintenance.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Peter Lansdorp
Summary: The number of telomere repeats varies greatly between chromosomes, cells, and species. Loss of telomere repeats limits cell proliferation and contributes to aging, but the mechanisms behind these processes are still not fully understood. Further research is needed to investigate the role of factors such as damaged telomeric DNA, replication errors, chromatin structure, and secondary DNA structures in telomere dynamics in different cell types.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Xin Zhang, Mengting Shi, Xi Zhao, Ennan Bin, Yucheng Hu, Nan Tang, Huaping Dai, Chen Wang
Summary: This study aimed to investigate the regeneration capacity of AT2 cells with short telomeres. It was found that short telomeres in AT2 cells result in reduced expression level of TPPP3, thereby limiting the differentiation and regeneration capacity of AT2 cells.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Freddy Khayat, Elda Cannavo, Majedh Alshmery, William R. Foster, Charly Chahwan, Martino Maddalena, Christopher Smith, Anthony W. Oliver, Adam T. Watson, Antony M. Carr, Petr Cejka, Alessandro Bianchi
Summary: In budding yeast, the activity of the MRX complex in DNA repair and DNA damage signaling is inhibited by telomeric protein Rif2 via a short motif at the N-terminus. This indicates a mechanism to suppress the DNA damage response at chromosome ends.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ryan P. Barnes, Mariarosaria de Rosa, Sanjana A. Thosar, Ariana C. Detwiler, Vera Roginskaya, Bennett Van Houten, Marcel P. Bruchez, Jacob Stewart-Ornstein, Patricia L. Opresko
Summary: This study reveals how oxidative stress induces significant cellular aging by generating oxidative damage at telomeres. The mechanism through which oxidative damage triggers cellular senescence is not through telomere shortening, but through inducing telomere fragility, leading to accelerated cellular aging.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xu Li, Meijie Wang, Wei Zheng, Wei Huang, Zeyu Wang, Kairang Jin, Lin Liu, Zhongbo Yu
Summary: Chromosome stability is primarily determined by telomere length, which is regulated by the core subunit TRF1 of shelterin. Research on the dynamics of TRF1 has shown its role in telomere organization, compaction, and interaction at telomeric DNA forks. Understanding these mechanisms can facilitate future studies on telomeres and the development of shelterin-targeted drugs.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Genetics & Heredity
Lara Perez-Martinez, Tina Wagner, Brian Luke
Summary: In this mini-review, the authors used a proteomics approach to identify potential RNA regulators associated with telomeric sequences. They found proteins that may positively or negatively regulate TERRA depending on the state of the telomere. These findings expand our understanding of the dynamics of telomere shortening in relation to TERRA and telomere interactors.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Xupeng Yu, Sean Gray, Helder C. Ferreira
Summary: The POT-3 protein in Caenorhabditis elegans is found to bind the telomeric G-strand and affects telomere length and telomeric C-circle formation, showing differences with the closely related POT-2 protein in terms of binding sites and function.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jean Chatain, Georges Hatem, Emmanuelle Delagoutte, Jean-Francois Riou, Patrizia Alberti, Carole Saintome
Summary: Telomeres are repetitive DNA sequences that protect the ends of eukaryotic chromosomes by associating with shelterin proteins. In potassium solutions, the telomeric G4 structure can be bound by hPOT1, showing a preference for binding from 3' to 5'. The cooperative binding of hPOT1-TPP1 in potassium is due to the structuring of telomeric DNA into G4, rather than direct protein-protein interactions.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Golam Mustafa, Sajad Shiekh, G. C. Keshav, Sanjaya Abeysirigunawardena, Hamza Balci
Summary: The accessibility of telomeric DNA is influenced by its length, with longer telomeres forming more compact structures that are less accessible to binding molecules. Intermediate regions of telomeric DNA were found to be more accessible compared to the ends, suggesting a gradient of accessibility along the telomere length. These findings highlight the importance of telomere structure in regulating accessibility to critical genomic sites.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hai Pan, Parminder Kaur, Ryan Barnes, Ariana C. Detwiler, Samantha Lynn Sanford, Ming Liu, Pengning Xu, Chelsea Mahn, Qingyu Tang, Pengyu Hao, Dhruv Bhattaram, Changjiang You, Xinyun Gu, Warren Lu, Jacob Piehler, Guozhou Xu, Keith Weninger, Robert Riehn, Patricia L. Opresko, Hong Wang
Summary: This study reveals that the interaction between TIN2 and TRF1 promotes DNA compaction and DNA-DNA bridging in a telomeric sequence- and length-dependent manner. Short telomeric DNA substrates exhibit transient TRF1-mediated telomeric DNA-DNA bridging events, while longer substrates show stable TRF1-mediated DNA-DNA bridging events. Tankyrase 1 and NAD+ can modulate TRF1-TIN2 mediated DNA-DNA bridging, while TPP1 inhibits TRF1-TIN2L-mediated DNA-DNA bridging. These findings suggest a molecular model where protein assemblies at telomeres are heterogeneous with distinct subcomplexes and play different roles in telomere protection and elongation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Environmental Sciences
Pheerasak Assavanopakun, Ratana Sapbamrer, Sirinart Kumfu, Nipon Chattipakorn, Siriporn C. Chattipakorn
Summary: Air pollution poses a major threat to health, affecting cellular senescence and oxidative stress. Telomere length serves as a useful biomarker for early detection of health issues related to air pollution exposure. Different factors influence the association between telomere length and air pollution, with even low levels of exposure causing alterations.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Rita Eid, Marie-Veronique Demattei, Harikleia Episkopou, Corinne Auge-Gouillou, Anabelle Decottignies, Nathalie Grandin, Michel Charbonneau
MOLECULAR AND CELLULAR BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Eric Le Balc'h, Nathalie Grandin, Marie-Veronique Demattei, Serge Guyetant, Anne Tallet, Jean-Christophe Pages, Mehdi Ouaissi, Thierry Lecomte, Michel Charbonneau
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Oncology
Anne Fogli, Marie-Veronique Demattei, Laetitia Corset, Catherine Vaurs-Barriere, Emmanuel Chautard, Julian Biau, Jean-Louis Kemeny, Catherine Godfraind, Bruno Pereira, Toufik Khalil, Nathalie Grandin, Philippe Arnaud, Michel Charbonneau, Pierre Verrelle
JOURNAL OF NEURO-ONCOLOGY
(2017)
Article
Genetics & Heredity
Nathalie Grandin, Michel Charbonneau
Article
Multidisciplinary Sciences
Nathalie Grandin, Laetitia Corset, Michel Charbonneau
Article
Biochemistry & Molecular Biology
Olga Calvo, Nathalie Grandin, Antonio Jordan-Pla, Esperanza Minambres, Noelia Gonzalez-Polo, Jose E. Perez-Ortin, Michel Charbonneau
NUCLEIC ACIDS RESEARCH
(2019)
Article
Neurosciences
Nathalie Grandin, Bruno Pereira, Camille Cohen, Pauline Billard, Caroline Dehais, Catherine Carpentier, Ahmed Idbaih, Franck Bielle, Francois Ducray, Dominique Figarella-Branger, Jean-Yves Delattre, Marc Sanson, Patrick Lomonte, Delphine Poncet, Pierre Verrelle, Michel Charbonneau, C. Desenclos, H. Sevestre, P. Menei, A. Rousseau, T. Cruel, S. Lopez, M-I Mihai, A. Petit, C. Adam, F. Parker, P. Dam-Hieu, I. Quintin-Roue, S. Eimer, H. Loiseau, L. Bekaert, F. Chapon, D. Ricard, C. Godfraind, T. Khalil, D. Cazals-Hatem, T. Faillot, C. Gaultier, M. C. Tortel, I. Carpiuc, P. Richard, W. Lahiani, H. Aubriot-Lorton, F. Ghiringhelli, C. A. Maurage, C. Ramirez, E. M. Gueye, F. Labrousse, O. Chinot, L. Bauchet, P. Beauchesne, G. Gauchotte, M. Campone, D. Loussouarn, D. Fontaine, F. Vandenbos-Burel, A. Le Floch, P. Roger, C. Blechet, M. Fesneau, A. Carpentier, J. Y. Delattre, S. Elouadhani-Hamdi, M. Polivka, D. Larrieu-Ciron, S. Milin, P. Colin, M. D. Diebold, D. Chiforeanu, E. Vauleon, O. Langlois, A. Laquerriere, F. Forest, M. J. Motso-Fotso, M. Andraud, G. Runavot, B. Lhermitte, G. Noel, S. Gaillard, C. Villa, N. Desse, C. Rousselot-Denis, I. Zemmoura, E. Cohen-Moyal, E. Uro-Coste, F. Dhermain
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2019)
Article
Genetics & Heredity
Nathalie Grandin, Maria Eugenia Gallego, Charles White, Michel Charbonneau
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.