Article
Biochemistry & Molecular Biology
Katarina Grossmannova, Monika Barathova, Petra Belvoncikova, Viliam Lauko, Lucia Csaderova, Jan Tomka, Tomas Dulka, Jaromir Pastorek, Juraj Madaric
Summary: Abdominal aortic aneurysms (AAA) are a significant cause of premature deaths worldwide. In this study, the presence of hypoxia-related protein CA IX in AAA tissues was confirmed, suggesting its potential role in aneurysmal wall remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Barbora Puzderova, Petra Belvoncikova, Katarina Grossmannova, Lucia Csaderova, Martina Labudova, Silvia Fecikova, Jaromir Pastorek, Monika Barathova
Summary: Resistance to chemotherapy is a major obstacle in cancer treatment. The non-selective beta-blocker propranolol has the potential to overcome drug resistance by disrupting tumor adaptation and activating apoptosis. It can serve as a chemosensitizer in combined therapy to disrupt tumor microenvironment homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jingjing Wang, Zhaoli Sun, Shuren Wang, Chenyang Zhao, Junjie Xu, Shen Gao, Meng Yang, Fugeng Sheng, Song Gao, Yanglong Hou
Summary: The study introduces a tumor therapy strategy that disrupts intratumoral metabolic symbiosis by inducing acidosis for tumor elimination, using carbonic anhydrase inhibitor-modified ferrous sulfide nanoparticles. The nanoparticles also have dual-mode imaging capabilities.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Ririko Nakaoka, Kazuhiro Kato, Kumiko Yamamoto, Hironobu Yasui, Shingo Matsumoto, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata
Summary: Extracellular acidification is a hallmark of tumor malignancy, and non-invasive mapping of extracellular pH (pHe) is essential for understanding the tumor microenvironment and monitoring tumor response to metabolism-targeting drugs. A modified electron paramagnetic resonance (EPR) system achieves higher sensitivity in mapping pHe and can be used to monitor the response of other metabolism-targeting drugs.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jun Yan, Kaizhen Wang, Lijuan Gui, Xian Liu, Yingying Ji, Jingjing Lin, Man Luo, Hong Xu, Jingxuan Lv, Fang Tan, Liangting Lin, Zhenwei Yuan
Summary: Nitroreductase (NTR) is an enzyme that is upregulated under tumor-depleted oxygen conditions. In this study, we developed a novel near-infrared (NIR) fluorescent probe IR-ABS that jointly targets NTR and carbonic anhydrase IX (CAIX). The probe showed excellent sensitivity and selectivity and demonstrated good biosafety in both cells and mice. Furthermore, the probe was successfully applied for NTR detection in orthotopic lung tumor models.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Mohammed Al-Zubaidi, Pravin Viswambaram, Steve McCombie, Elizabeth Liow, Nat Lenzo, Tom Ferguson, Andrew D. Redfern, Richard Gauci, Dickon Hayne
Summary: Bladder cancer is a lethal disease with limited conventional imaging modalities for accurate staging. This study aims to investigate the use of (89)Zirconium-labelled girentuximab PET in the staging of bladder and urothelial carcinomas.
Article
Medicine, Research & Experimental
Daan F. Boreel, Paul N. Span, Annemarie Kip, Milou Boswinkel, Johannes P. W. Peters, Gosse J. Adema, Johan Bussink, Sandra Heskamp
Summary: Limited oxygen diffusion and increased oxygen consumption in solid malignancies lead to chronic hypoxia, which induces radioresistance and an immunosuppressive microenvironment. This study aimed to develop a radiolabeled antibody to visualize chronic hypoxia and study immune cell populations in these hypoxic areas. The results showed that the radiotracer binds to CAIX-expressing cells in vitro and accumulates in CAIX+ areas in vivo. The technique can quantitatively distinguish between tumor models with varying CAIX+ fractions and identified less infiltration of immune cells in the CAIX+ areas.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Ozlem Akgul, Srishti Singh, Jacob T. Andring, Robert McKenna, Silvia Selleri, Fabrizio Carta, Andrea Angeli, Claudiu T. Supuran
Summary: A series of taurine substituted sulfonamide derivatives with ureido moiety at the tail section were synthesized as selective inhibitors of tumor associated human Carbonic Anhydrase (CA) IX and XII. These derivatives showed a strong dependence on the presence of ureido moiety and demonstrated highly stabilized ligand-protein complex with specific amino acid residues in both hCA II and hCA IX-mimic.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Oncology
Miriam Zatovicova, Ivana Kajanova, Monika Barathova, Martina Takacova, Martina Labudova, Lucia Csaderova, Lenka Jelenska, Eliska Svastova, Silvia Pastorekova, Adrian L. Harris, Jaromir Pastorek
Summary: Hypoxia in the tumor microenvironment is a major factor in cancer progression, and low levels of oxygen may indicate treatment failure. Carbonic anhydrase IX (CA IX) is a precise target in hypoxic tumors, and its presence contributes to the failure of standard therapy. Humanized antibodies against CA IX show promise as suitable therapies for hypoxic tumors, with the ability to modulate the tumor microenvironment, induce an immune response, and block metastasis formation.
CANCER & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Md. Abu Sufian, Sabina Zamanova, Ahmed M. Shabana, Brianna Kemp, Utpal K. Mondal, Claudiu T. Supuran, Marc A. Ilies
Summary: Carbonic anhydrase IX (CA IX) is an important enzyme involved in tumor survival, metastasis, and resistance to therapy in hypoxic tumor cells. The expression dynamics of CA IX in different oxygen conditions were investigated, along with its correlation with extracellular pH and cell viability. The study found that CA IX expression is retained in hypoxic cancer cells upon reoxygenation to preserve their proliferation ability. The sensitivity of cancer cells to CA IX inhibitors is higher in hypoxia and intermittent hypoxia compared to normoxia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Julia C. Carmona-Bozo, Roido Manavaki, Jodi L. Miller, Cara Brodie, Corradina Caraco, Ramona Woitek, Gabrielle C. Baxter, Martin J. Graves, Tim D. Fryer, Elena Provenzano, Fiona J. Gilbert
Summary: This study explores the relationship between indices of hypoxia and vascular function in ER + breast cancer using [F-18]-FMISO PET/MRI and immunohistochemical markers. The results indicate that hypoxia is associated with increased CAIX expression, reduced microvessel density, and smaller vessel diameters in ER + breast cancer, further confirming the link between inadequate vascularity and hypoxia in this type of cancer.
EUROPEAN RADIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Thitima Pewklang, Kantapat Chansaenpak, Siti Nursyahirah Bakar, Rung-Yi Lai, Chin Siang Kue, Anyanee Kamkaew
Summary: AZB-I-CAIX(2) was developed as a photosensitizer targeting CAIX, showing specific affinity and enhanced photocytotoxicity in CAIX-expressed cancer cells, which could reduce tumor size by preventing PDT-induced hypoxia through CAIX inhibition.
FRONTIERS IN CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Paul C. McDonald, Shoukat Dedhar
Summary: Tumour-associated carbonic anhydrases (CA) IX and XII are upregulated by cancer cells to combat stress caused by hypoxia and acidosis in solid tumours. Targeting CAIX activity with SLC-0111, a selective CAIX/XII inhibitor, has shown anti-tumour efficacy in multiple solid tumour models, especially when used in combination with chemotherapy or immune checkpoint blockade. However, sustainability and durability of CAIX inhibition is limited by adaptive resistance, leading to tumour recurrence. CAIX inhibition may sensitize tumour cells to cancer therapy by inducing iron-dependent phospholipid peroxidation and subsequent ferroptotic cell death.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Oncology
Katja Eloranta, Marjut Pihlajoki, Emmi Liljestroem, Ruth Nousiainen, Tea Soini, Jouko Lohi, Stefano Cairo, David B. Wilson, Seppo Parkkila, Markku Heikinheimo
Summary: Hypoxia induces the expression of CAIX in hepatoblastoma (HB) cells, and the CAIX inhibitor SLC-0111 shows in vitro activity against these malignant cells by decreasing cell viability and motility.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ingeborg Rezuchova, Maria Bartosova, Petra Belvoncikova, Martina Takacova, Miriam Zatovicova, Lenka Jelenska, Lucia Csaderova, Iveta Meciarova, Kamil Pohlodek
Summary: Carbonic anhydrase IX (CA IX) is a reliable marker for hypoxia and a poor prognostic factor in solid tumors, including breast cancer. This study presents two new diagnostic tools-a monoclonal antibody for CA IX detection by immunohistochemistry and an ELISA kit for sCA IX detection in plasma-validated on a cohort of 100 early breast cancer patients. Tissue CA IX positivity correlates with tumor grading, necrosis, negative hormone receptor status, and the TNBC subtype, while the ELISA test provides good sensitivity and specificity. The amount of sCA IX depends on the subcellular localization of CA IX and the molecular composition of different breast cancer subtypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Hidehiro Okura, Brian J. Golbourn, Uswa Shahzad, Sameer Agnihotri, Nesrin Sabha, Jonathan R. Krieger, Carlyn A. Figueiredo, Alan Chalil, Natalie Landon-Brace, Alexandra Riemenschneider, Hajime Arai, Christian A. Smith, Songli Xu, Stefan Kaluz, Adam I. Marcus, Erwin G. Van Meir, James T. Rutka
Article
Oncology
Xin Dai, Stefan Kaluz, Ying Jiang, Lei Shi, DeAngelo Mckinley, Yingzhe Wang, Binghe Wang, Erwin G. Van Meir, Chalet Tan
Editorial Material
Cell Biology
Stefan Kaluz, Chalet Tan, Erwin G. Van Meir
Article
Chemistry, Medicinal
Chalet Tan, Rita G. de Noronha, Narra S. Devi, Adnan A. Jabbar, Stefan Kaluz, Yuan Liu, Suazette Reid Mooring, K. C. Nicolaou, Binghe Wang, Erwin G. Van Meir
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2011)
Article
Oncology
Norazizah Shafee, Stefan Kaluz, Ning Ru, Eric J. Stanbridge
Article
Oncology
Jia-ning Cao, Norazizah Shafee, Larry Vickery, Stefan Kaluz, Ning Ru, Eric J. Stanbridge
Article
Oncology
Shaoman Yin, Stefan Kaluz, Narra S. Devi, Adnan A. Jabbar, Rita G. de Noronha, Jiyoung Mun, Zhaobin Zhang, Purushotham R. Boreddy, Wei Wang, Zhibo Wang, Thomas Abbruscato, Zhengjia Chen, Jeffrey J. Olson, Ruiwen Zhang, Mark M. Goodman, K. C. Nicolaou, Erwin G. Van Meir
CLINICAL CANCER RESEARCH
(2012)
Review
Chemistry, Medicinal
Sarah K. Burroughs, Stefan Kaluz, Danzhu Wang, Ke Wang, Erwin G. Van Meir, Binghe Wang
FUTURE MEDICINAL CHEMISTRY
(2013)
Article
Chemistry, Medicinal
Suazette Reid Mooring, Hui Jin, Narra S. Devi, Adnan A. Jabbar, Stefan Kaluz, Yuan Liu, Erwin G. Van Meir, Binghe Wang
JOURNAL OF MEDICINAL CHEMISTRY
(2011)
Editorial Material
Genetics & Heredity
Stefan Kaluz, Erwin G. Van Meir
JOURNAL OF MOLECULAR MEDICINE-JMM
(2011)
Article
Biochemistry & Molecular Biology
Jun Fan, Changliang Shan, Hee-Bum Kang, Shannon Elf, Jianxin Xie, Meghan Tucker, Ting-Lei Gu, Mike Aguiar, Scott Lonning, Huaibin Chen, Moosa Mohammadi, Laura-Mae P. Britton, Benjamin A. Garcia, Masa Aleckovic, Yibin Kang, Stefan Kaluz, Nara Devi, Erwin G. Van Meir, Taro Hitosugi, Jae Ho Seo, Sagar Lonial, Manila Gaddh, Martha Arellano, Hanna J. Khoury, Fadlo R. Khuri, Titus J. Boggon, Sumin Kang, Jing Chen
Article
Biochemistry & Molecular Biology
S. M. Cork, B. Kaur, N. S. Devi, L. Cooper, J. H. Saltz, E. M. Sandberg, S. Kaluz, E. G. Van Meir
Article
Chemistry, Medicinal
Qi Shi, Shaoman Yin, Stefan Kaluz, Nanting Ni, Narra Sarojini Devi, Jiyoung Mun, Danzhu Wang, Krishna Damera, Weixuan Chen, Sarah Burroughs, Suazette Reid Mooring, Mark M. Goodman, Erwin G. Van Meir, Binghe Wang, James P. Snyder
ACS MEDICINAL CHEMISTRY LETTERS
(2012)
Article
Oncology
Lei Dong, Shuo You, Qing Zhang, Satoru Osuka, Narra S. Devi, Stefan Kaluz, Jalisa H. Ferguson, Hua Yang, Guoliang Chen, Binghe Wang, Hans E. Grossniklaus, Erwin G. Van Meir
CLINICAL CANCER RESEARCH
(2019)