Article
Biochemistry & Molecular Biology
Ahmet Tuncay, Anna Noble, Matthew Guille, James N. Cobley
Summary: RedoxiFluor is a novel antibody technology that enables accurate quantification of target-specific protein thiol redox state in percentages and moles on a microplate. It can operate in global mode and array mode, making it highly versatile. Extensive validation in diverse biological contexts demonstrates the reliability and utility of this technology.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Marzieh Ahangarpour, Iman Kavianinia, Paul A. Hume, Paul W. R. Harris, Margaret A. Brimble
Summary: In this study, we report the first examples of thiol-selective heterobifunctional electrophiles, N-vinyl acrylamides, which enable efficient and highly selective thiol-thiol bioconjugations and cysteine modification. We demonstrate that these new thiol-selective scaffolds can undergo chemical reactions under biocompatible conditions. Computational studies explain an unexpected addition reaction. The study also reveals that N-methylation of the N-vinyl acrylamide scaffold can change the regioselectivity of the reaction.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Organic
Jimin Guo, Jianjian Zha, Tao Zhang, Chang-Hua Ding, Qitao Tan, Bin Xu
Summary: Unsymmetrical disulfides can be prepared effectively through thiol exchange with symmetrical disulfides using a simple PdCl2/DMSO catalytic system. The method demonstrates excellent functional group tolerance, a broad substrate scope, and operational simplicity. This reaction is particularly valuable for late-stage functionalization of bioactive scaffolds such as peptides and pharmaceuticals.
Article
Biochemistry & Molecular Biology
Xiaoyang Chen, Qiutao Xu, Yaping Yue, Yuhang Duan, Hao Liu, Xiaolin Chen, Junbin Huang, Lu Zheng
Summary: Pathogen attack increases reactive oxygen species (ROS) levels in plants, which activate plant defense mechanisms. Our study used a quantitative proteomics approach to map oxidized cysteine sites in rice leaves and identified proteins involved in various biological processes, including defense response pathways. We found that infection by Magnaporthe oryzae increased oxidative modification levels of cysteine sites in ribosomal proteins, promoting the translational efficiency of mRNAs involved in defense responses. Our findings reveal a novel role of post-translational modifications and provide valuable resources for future research on protein redox regulation.
PLANT COMMUNICATIONS
(2023)
Article
Chemistry, Organic
Amit Chakraborty, Fernando Albericio, Beatriz G. de la Torre
Summary: Chemoselective disulfide formation is achieved through thiol-disulfide interchange approach using sec-isoamyl mercaptan (SIT) as an alkyl sulfenyl-protecting group. SIT acts as a masking group during synthesis and directs disulfide formation in the presence of a free thiol. This novel approach is demonstrated by synthesizing several peptides of biological interest.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Polymer Science
Taylor Wright, Ziyue Zhang, Carl A. Michal, Savvas G. Hatzikiriakos, Michael O. Wolf
Summary: The study demonstrates the photo-oxidation of thiol functionalities into disulfide cross-links using a telluride catalyst and photo-generated singlet oxygen, resulting in degradable elastic materials. The kinetics of the process are influenced by the telluride concentration for cross-linking rate and the rate of telluride oxidation for cross-linking onset.
Article
Plant Sciences
Ying Lu, Li Yuan, Zhou Zhou, Mengyu Wang, Xiaoyun Wang, Shizhong Zhang, Qinghua Sun
Summary: The study demonstrated that the anti-stress function of AtPDI1 in Arabidopsis is directly related to its disulfide isomerase activity. Mutant plants with altered cysteine residues showed significantly lower germination rates under stress conditions compared to wild-type and overexpressing lines. The transcriptional levels of stress-responsive genes were also lower in the mutant plants, indicating the importance of AtPDI1 in stress response.
Article
Chemistry, Multidisciplinary
Yu Chen, Jiafu Shi, Yizhou Wu, Zheyuan Guo, Shihao Li, Wenping Li, Zhenhua Wu, Hongjian Wang, Haifei Jiang, Zhongyi Jiang
Summary: Photosynthesis can be used for the recycling of NADH in bio-redox reactions. By designing the oxidation of biomass derivatives as an electron supply module and using SOD/CAT cascade catalysis as a ROS elimination module, the NADH degradation can be inhibited, leading to an increase in NADH yield and reaction rate. This NADH photosynthesis system provides a novel and efficient platform for biomass-to-chemical conversion.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Natalia Zamorano Cuervo, Nathalie Grandvaux
Summary: Protein disulfide isomerases (PDIs) are enzymes that catalyze redox reactions and have chaperone functions in protein folding. PDIs have catalytic thioredoxin (TRX)-like domains with CXXC motifs for redox reactions, as well as non-catalytic TRX-like domains. Strategies for identifying cysteine oxidative post-translational modifications (Cys ox-PTMs) have been optimized, and analysis of a Cys redoxome dataset suggests the presence of a non-catalytic thiol-disulfide motif in PDIs. Further structural analysis and exploration of other PDIs are needed to understand the role of these motifs.
Article
Nanoscience & Nanotechnology
Ziqiang Zhou, Yanfang Wang, Ping Hu
Summary: A new approach based on FRET mechanism was reported for monitoring drug release and inflammatory reactions in vitro and in vivo. Mixed micelles containing a fluorescent donor and its quencher were prepared and their stability and responsiveness towards oxidants were tested. The micelles were able to restore fluorescence and visualize inflammatory cells and reactions.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Food Science & Technology
Zhaoming Wang, Hui Zhou, Kai Zhou, Juncai Tu, Baocai Xu
Summary: This study found that during aging of rabbit meat, PSE-like meat samples showed significantly higher values in peroxides, protein oxidation and degradation compared to normal meat samples, indicating that the ROS-generating system played a potential role in the softening process of PSE-like meat.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Kinga Kik, Bozena Bukowska, Anita Krokosz, Paulina Sicinska
Summary: The study evaluated the effects of different diameters of polystyrene nanoparticles on oxidative stress and cell viability of human peripheral blood mononuclear cells. It showed that these nanoparticles disrupted the redox balance in cells, leading to oxidative stress and cell death. The toxicity and oxidative properties of the nanoparticles depended significantly on their diameter.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Qiping Song, Xipan Wang, Yang Liu, Marian Brestic, Xinghong Yang
Summary: In this study, we identified a VKOR-like protein called lumen thiol oxidoreductase 1 (StLTO1) in the thylakoid membrane of Solanum tuberosum L. Overexpression of StLTO1 in potato plants enhanced their cold tolerance by improving photosynthetic capacity, scavenging ROS, and regulating cold-responsive gene expression.
Article
Biochemistry & Molecular Biology
Elisabeth C. van der Slikke, Lisanne Boekhoud, Arno R. Bourgonje, Tycho J. Olgers, Jan C. ter Maaten, Robert H. Henning, Harry van Goor, Hjalmar R. Bouma
Summary: Oxidative stress plays an important role in the pathogenesis of sepsis-associated acute kidney injury. Lower plasma free thiol levels and increased levels of ischemia-modified albumin reflect the extent of oxidative stress. The study found that plasma free thiol levels at the emergency department were inversely associated with long-term renal function decline and survival rates.
Article
Plant Sciences
Jing Xin, Chuanling Li, Kexin Ning, Yuan Qin, Jian-Xiu Shang, Yu Sun
Summary: Protein phosphorylation, especially serine/threonine and tyrosine phosphorylation, is crucial for signaling pathways in plant growth, development, and stress responses. DSP3, an atypical dual-specificity protein tyrosine phosphatase, negatively affects salt stress responses in Arabidopsis by modulating phosphorylated MPK3 and MPK6 levels.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biology
Diana Aparicio-Bautista, Daniel Chavez-Valenzuela, Giovanni Ambriz-Alvarez, Teodoro Cordova-Fraga, Juan P. Reyes-Grajeda, Oscar Medina-Contreras, Fanny Rodriguez-Cruz, Francisco Garcia-Sierra, Patricia Zuniga-Sanchez, Antonio M. Gutierrez-Gutierrez, Jaime Arellanes-Robledo, Gustavo Basurto-Islas
Summary: In this study, an ELF-VMF-modified Rodin's coil was designed to expose cells, and the effects on the cell proteome and structure were analyzed. The results showed that ELF-VMFs at specific frequency and exposure time altered the cell proteome and structurally affected the target cells.
BIOELECTROMAGNETICS
(2022)
Article
Cell Biology
Jovito Cesar Santos-Alvarez, Juan Manuel Velazquez-Enriquez, Rosendo Garcia-Carrillo, Cesar Rodriguez-Beas, Alma Aurora Ramirez-Hernandez, Edilburga Reyes-Jimenez, Karina Gonzalez-Garcia, Armando Lopez-Martinez, Laura Perez-Campos Mayoral, Sergio Roberto Aguilar-Ruiz, Maria de los Angeles Romero-Tlalolini, Honorio Torres-Aguilar, Luis Castro-Sanchez, Jaime Arellanes-Robledo, Veronica Rocio Vasquez-Garzon, Rafael Baltierrez-Hoyos
Summary: This study characterized differentially expressed miRNAs in extracellular vesicles (EVs) obtained from different cell lines isolated from IPF lungs and identified potential biomarkers for IPF fibrogenesis, diagnosis, and therapeutic intervention.
Review
Biochemistry & Molecular Biology
Osiris German Idelfonso-Garcia, Brisa Rodope Alarcon-Sanchez, Veronica Rocio Vasquez-Garzon, Rafael Baltierrez-Hoyos, Saul Villa-Trevino, Pablo Muriel, Hector Serrano, Julio Isael Perez-Carreon, Jaime Arellanes-Robledo
Summary: Nucleoredoxin (NXN), an oxidoreductase enzyme, regulates multiple signaling pathways and plays a role in maintaining cellular redox homeostasis. It is involved in various cellular processes and may be associated with different diseases.
Article
Gastroenterology & Hepatology
Eduardo E. Vargas-Pozada, Erika Ramos-Tovar, Juan D. Rodriguez-Callejas, Irina Cardoso-Lezama, Silvia Galindo-Gomez, Karla Gil-Becerril, Veronica Rocio Vasquez-Garzon, Jaime Arellanes-Robledo, Victor Tsutsumi, Saul Villa-Trevino, Pablo Muriel
Summary: The study aimed to investigate the relevance of nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) inflammasome activation in the development of nonalcoholic steatohepatitis (NASH) induced by carbon tetrachloride (CCl4) along with an hepatopathogenic diet. The results showed that activation of the NLRP3 inflammasome triggered by low doses of CCl4 exacerbated the severity of NASH, indicating the important role of NLRP3 inflammasome activation in the development of NASH.
ANNALS OF HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eduardo E. Vargas-Pozada, Erika Ramos-Tovar, Juan D. Rodriguez-Callejas, Irina Cardoso-Lezama, Silvia Galindo-Gomez, Daniel Talamas-Lara, Veronica Rocio Vasquez-Garzon, Jaime Arellanes-Robledo, Victor Tsutsumi, Saul Villa-Trevino, Pablo Muriel
Summary: This study evaluated the effect of caffeine on the NLRP3 inflammasome signaling pathway in a rat model of NASH. The findings suggest that caffeine inhibits NLRP3 inflammasome activation by suppressing the TLR4/MAPK/NF-κB signaling pathway, thereby reducing fat accumulation, necrosis, cholestasis, oxidative stress, and fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Biochemistry & Molecular Biology
Dulce Maria Moreno-Garcia, Armando Zepeda-Bastida, Juan Ocampo-Lopez, Brisa Rodope Alarcon-Sanchez, Osiris German Idelfonso-Garcia, Sandra Rosas-Madrigal, Diana Ivette Aparicio-Bautista, Julio Isael Perez-Carreon, Saul Villa-Trevino, Jaime Arellanes-Robledo
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2023)
Review
Gastroenterology & Hepatology
Fernanda Villarruel-Melquiades, Maria Eugenia Mendoza-Garrido, Claudia M. Garcia-Cuellar, Yesennia Sanchez-Perez, Julio Isael Perez-Carreon, Javier Camacho
Summary: Hepatocellular carcinoma (HCC) is a highly lethal malignancy with a high mortality-to-incidence ratio worldwide. First-line drugs such as sorafenib and lenvatinib are ineffective in most cases due to late diagnosis and tumour resistance. New pharmacological approaches, including immune checkpoint inhibitors and monoclonal antibodies against programmed cell death-1, as well as drug combinations and repurposing, offer promising alternatives for HCC treatment.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Multidisciplinary Sciences
Ema Elvira Herrera-Lopez, Dafne Guerrero-Escalera, Isaac Aguirre-Maldonado, Arely Lopez-Hernandez, Hilda Montero, Maria Angelica Gutierrez-Nava, Luis del Pozo-Yauner, Jaime Arellanes-Robledo, Javier Camacho, Julio Isael Perez-Carreon
Summary: Hepatocellular carcinoma (HCC) is a highly lethal liver cancer with late diagnosis. This study identified Annexin A1 and A5 as potential early biomarkers for poor prognosis in HCC patients. Annexin A5 levels in plasma were significantly correlated with Annexin A5 levels in liver tissue and gradually increased from week 12 to week 22.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Maria Stephanie Cid-Gallegos, Cristian Jimenez-Martinez, Xariss M. Sanchez-Chino, Eduardo Madrigal-Bujaidar, Veronica R. Vasquez-Garzon, Rafael Baltierrez-Hoyos, Isela Alvarez-Gonzalez
Summary: This study evaluates the chemopreventive effect of chickpea on colon carcinogenesis in a mice model. The results show that a 20% chickpea diet significantly reduces tumors and biomarkers of proliferation and inflammation in AOM/DSS-induced colon cancer mice. Both diets of 10% and 20% chickpea exert a chemopreventive effect.
Article
Biochemistry & Molecular Biology
Rafael Nambo-Venegas, Berenice Palacios-Gonzalez, Jaime Mas-Oliva, Ana Karen Aurioles-Amozurrutia, Armando Cruz-Rangel, Abel Moreno, Alfredo Hidalgo-Miranda, Mauricio Rodriguez-Dorantes, Felipe Vadillo-Ortega, Juan Xicohtencatl-Cortes, Maria Isabel Ruiz-Olmedo, Juan Pablo Reyes-Grajeda
Summary: This study aimed to investigate the changes in the transcriptome of M1 macrophages during the internalization of LDL particles and its relationship with sex. It was found that male macrophages exhibited more pronounced proinflammatory gene expression during LDL internalization, while female macrophages showed a higher number of markers associated with cell damage.
Article
Nutrition & Dietetics
Jaime Morales-Romero, Maria Cristina Ortiz Leon, Hector Hernandez-Gutierrez, Roberto A. Bahena-Ceron, Aide Miranda-Reza, Jose A. Marin-Carmona, Edit Rodriguez-Romero, Silvia I. Mora-Herrera, Jonathan Garcia-Roman, Julio I. Perez-Carreon, Eduardo Rivadeneyra-Dominguez, Gabriel Riande-Juarez, Rebeca Garcia-Roman
Summary: This study evaluated the effect of high-methionine dietary therapy on patients with NAFLD and found that nutritional therapy can reduce the severity of NAFLD, central fat accumulation, blood pressure, and the fatty liver index, while improving the quality of life of patients.
ARCHIVOS LATINOAMERICANOS DE NUTRICION
(2023)
Review
Immunology
Luis Del Pozo-Yauner, Guillermo A. Herrera, Julio Perez I. Carreon, Elba A. Turbat-Herrera, Francisco J. Rodriguez-Alvarez, Robin Ruiz A. Zamora
Summary: The adaptive immune system of jawed vertebrates generates highly diverse antibodies through gene recombination and somatic hypermutation. However, the correct folding and assembly of the mutated heavy and light chains are crucial for antigen recognition. In patients with monoclonal gammopathies, overproduction of light chains can lead to misfolding and aggregation due to destabilizing somatic mutations.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Respiratory System
Juan Manuel Velazquez-Enriquez, Itayetzi Reyes-Avendano, Jovito Cesar Santos-Alvarez, Edilburga Reyes-Jimenez, Veronica Rocio Vasquez-Garzon, Rafael Baltierrez-Hoyos
Summary: This study identified differentially expressed genes (DEGs) related to idiopathic pulmonary fibrosis (IPF), including upregulated and downregulated genes. Additionally, 10 hub genes with high connectivity were discovered, suggesting their potential role in the development and progression of IPF. These hub genes were also found to be expressed in lung cancer and associated with various stages of cancer progression, indicating a possible link between IPF and lung cancer.
ADVANCES IN RESPIRATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)