期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1824, 期 12, 页码 1351-1357出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2012.07.007
关键词
Escherichia coli; Heat resistance; Compatible solute; Trehalose; Ribosome denaturation; Differential scanning calorimetry
资金
- Alberta Meat and Livestock Agency
- Canada Research Chairs program
This study investigated the mechanisms of heat resistance in Escherichia coli AW1.7 by quantification of cytoplasmic solutes, determination of ribosome denaturation, and by determination of protein denaturation. To assess the contribution of heat shock proteins and compatible solutes, experiments were conducted after exposure to sublethal heat shock, and with cultures grown at NaCl concentrations ranging from 0 to 6%. Heat resistance of E. coli AW1.7 was compared to the heat sensitive E. coli GGG10 and a plasmid-cured, heat sensitive derivative of E. coli AW1.7 named E. coli AW1.7 Delta pHR1. Sublethal heat shock improved survival at 60 degrees C of E. coli GGG10 and AW1.7 Delta pHR1 but not of E. coli AW1.7. Addition of NaCl increased the heat resistance of all three strains, but only E. coli AW1.7 exhibited high heat resistance when grown in NaCl concentrations ranging from 2 to 6%. E. coli AW1.7 and GGG10 accumulated 16.1 +/- 0.8 and 8.8 +/- 0.8 mmol L-1 amino acids when grown at 0% NaCl. and 1.47 +/- 0.07 and 0.78 +/- 0.06 mmol L-1 carbohydrates when grown at 6% NaCl, respectively. Ribosome denaturation was determined by differential scanning calorimetry. After growth in the presence of 0% NaCl, the 30S subunit denatured at 63.7 +/- 0.8 degrees C and 60.7 +/- 0.3 degrees C in E. coli AW1.7 and GGG10, respectively. Fourier-transformed-infrared-spectroscopy did not indicate differences in protein denaturation between the strains during heating. In conclusion, heat resistance in E. coli AW1.7 correlates to ribosome stability at 60 degrees C and is dependent on accumulation of cytoplasmic solutes. (C) 2012 Elsevier B.V. All rights reserved.
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