期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1824, 期 6, 页码 833-841出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2012.03.014
关键词
Atrazine; PCB 153; MCF-7 human cell; 2D-DiGE; Subcellular fraction
资金
- FNR (Fonds National de la Recherche) [FNR/03/07/05]
Several man-made organic pollutants including polychlorinated biphenyls (PCBs) and several pesticides may exhibit endocrine disrupting (ED) properties. These ED molecules can be comparatively persistent in the environment, and have shown to perturb hormonal activity and several physiological functions. The objective of this investigation was to study the impact of PCB 153 and atrazine on human MCF-7 cells, and to search for marker proteins of their exposure. Cells were exposed to environmentally high but relevant concentrations of atrazine (200 ppb), PCB 153 (500 ppb), 17-beta estradiol (positive control, 10 nM) and DMSO (0.1%, negative control) for t = 36 h (n = 3 replicates/exposure group). Proteins from cell membrane and cytosol were isolated, and studied by 2D-DiGE. Differentially regulated proteins were trypsin-digested and identified by MALDI-ToF-ToF and NCBInr database. A total of 36 differentially regulated proteins (>vertical bar 1.5 vertical bar fold change, P<0.05) were identified in the membrane fraction and 22 in the cytosol, and were mainly involved in cell structure and in stress response, but also in xenobiotic metabolism. 67% (membrane) and 50% (cytosol) of differentially regulated proteins were more abundant following atrazine exposure whereas nearly 100% (membrane) and 45% (cytosol) were less abundant following PCB 153 exposure. Western blots of selected proteins (HSBP1, FKBP4, STMN1) confirmed 2D-DiGE results. This study emphasizes the numerous potential effects that ED compounds could have on exposed humans. (C) 2012 Elsevier B.V. All rights reserved.
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