Article
Biotechnology & Applied Microbiology
Javier Garcia-Pardo, Aleksandra E. Badaczewska-Dawid, Carlos Pintado-Grima, Valentin Iglesias, Aleksander Kuriata, Sebastian Kmiecik, Salvador Ventura
Summary: This study presents the A3D yeast database (A3DyDB), which provides an extensive catalog of aggregation propensity predictions for the S. cerevisiae proteome, allowing for the evaluation of the influence of mutations on protein stability and solubility.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Hisato Suzuki, Kana Aoki, Kenji Kurosawa, Kazuo Imagawa, Tatsuyuki Ohto, Mamiko Yamada, Toshiki Takenouchi, Kenjiro Kosaki, Tohru Ishitani
Summary: CTR9 gene mutations are associated with macrocephaly, motor delay, and intellectual disability. The mutant proteins showed stronger affinity to PAF1 protein, and knockout of ctr9 gene in zebrafish resulted in motor defects and enlargement of the telencephalon. Rescue experiments failed to restore normal function, and overexpression of human CTR9 mutant mRNA caused enlargement of telencephalon in zebrafish larvae.
HUMAN MOLECULAR GENETICS
(2022)
Article
Biochemical Research Methods
Tara K. Bartolec, Joshua J. Hamey, Andrew Keller, Juan D. Chavez, James E. Bruce, Marc. R. Wilkins
Summary: This study investigates the function of histidine protein methyltransferase Hpm1p in yeast and confirms its association with diverse phenotypes, including sugar metabolism regulation, through techniques such as mass spectrometry analysis.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Food Science & Technology
Juan Antonio Porras-Aguera, Jaime Moreno-Garcia, Teresa Garcia-Martinez, Juan Moreno, Juan Carlos Mauricio
Summary: The prise de mousse stage during the elaboration of sparkling wine through the traditional method involves a second fermentation in a sealed bottle, which significantly contributes to the unique organoleptic properties of these wines. This study characterizes the mitochondrial response of Saccharomyces cerevisiae strains used in sparkling wine production and found that CO2 overpressure negatively affects mitochondrion-related proteins. However, proteins involved in wine aromas and respiratory chain are found to be over-represented in sealed bottles, with differences in concentrations observed depending on time and CO2 overpressure parameters. Ethanol content during fermentation may be a key factor in these proteomic changes, highlighting the importance of further research in this area.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mrinal Sharma, Prranshu Yadav, Ankita Doshi, Hemang D. Brahmbhatt, C. Ratna Prabha
Summary: The study examined the effects of six double mutants derived from the ubiquitin mutant UbEP42 on cell functions, finding that the double mutation L50P-I61T had the most detrimental impact. Overall, these double mutations made cells more sensitive, and the negative effects under certain conditions could be compensated for by each other.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Water Resources
Mohaddeseh Zahmatkesh Anbarani, Afsaneh Esmaeili Nasrabadi, Ziaeddin Bonyadi
Summary: The aim of this study was to evaluate the efficiency of S. cerevisiae in removing PS from aqueous solutions. BBD was used to determine the optimal removal conditions. The maximum PS removal efficiency was 98.81% under optimized conditions. Based on the results, it can be concluded that S. cerevisiae can be used as a natural and environmentally friendly biocoagulant to remove PS.
APPLIED WATER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ngai Ting Chan, Junfeng Huang, Gui Ma, Hao Zeng, Kristine Donahue, Yidan Wang, Lingjun Li, Wei Xu
Summary: CTR9 is a scaffold subunit in PAFc complex and plays a crucial role in controlling H3K27me3 levels and genomic distribution mediated by PRC2. Loss of CTR9 leads to increased H3K27me3 levels, expanded recruitment of PRC2 on chromatin, and a shift from less active PRC2.2 to more active PRC2.1, resulting in hypersensitivity of breast cancer cells to PRC2 inhibitors.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Ngai Ting Chan, Ming-Song Lee, Yidan Wang, Jacques Galipeau, Wan-Ju Li, Wei Xu
Summary: This study reveals that CTR9 regulates the osteochondral lineage differentiation of hMSCs by counteracting EZH2 activity and regulating BMP-2 expression. CTR9 knockdown in hMSCs leads to defects in osteogenic differentiation, which can be partially rescued by EZH2 inhibitors. CTR9 also regulates the secretion and function of BMP-2.
Article
Food Science & Technology
Jitka Laurent, Nore Struyf, An Bautil, Albina Bakeeva, Mikolaj Chmielarz, Marika Lyly, Beatriz Herrera-Malaver, Volkmar Passoth, Kevin J. Verstrepen, Christophe M. Courtin
Summary: A diet low in FODMAPs can help reduce symptoms in patients with irritable bowel syndrome, but avoiding cereal grain products may lead to nutritional deficiencies. Developing low-FODMAP, high-fiber cereal grain products using K. marxianus in bread production can effectively reduce fructan levels and maintain fructan and fructose levels below the threshold for low-FODMAP products.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Genetics & Heredity
Raquel Sorribes-Dauden, Maria Teresa Martinez-Pastor, Sergi Puig
Summary: Iron is essential for eukaryotic organisms, and its deficiency can lead to anemia. Yeast cells use the Ccc1 transporter to detoxify and store excess iron. Deletion of the Nt domain of Ccc1 can increase iron accumulation in cells.
Article
Immunology
Francesca Soutter, Dirk Werling, Matthew Nolan, Tatiana Kuster, Elizabeth Attree, Virginia Marugan-Hernandez, Sungwon Kim, Fiona M. Tomley, Damer P. Blake
Summary: A cheap and easy-to-produce oral vaccine has been developed using Saccharomyces cerevisiae yeast expressing Eimeria tenella antigens. The vaccine effectively reduces the replication of Eimeria tenella and improves the growth of chickens, making it an important tool for controlling coccidiosis in chickens, particularly in low- and middle-income countries.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Bazile Ravoityte, Juliana Luksa, Ralf Erik Wellinger, Saulius Serva, Elena Serviene
Summary: This study analyzed the impact of L-A virus on gene expression in yeast hosts using high-throughput RNA sequencing data. It was found that the presence of L-A virus resulted in moderate alterations in gene expression, particularly in genes involved in ribosome biogenesis. Additionally, the transcriptional responses to L-A maintenance were similar to those induced by stress or nutrient availability. The study also identified yeast transcriptional regulators that modulate the levels of L-A dsRNA in cells.
Review
Biotechnology & Applied Microbiology
R. Sahaya Glingston, Jyoti Yadav, Jitika Rajpoot, Neha Joshi, Shirisha Nagotu
Summary: Yeast has been proven to be a valuable model system for studying viral infection and expression of viral proteins, advancing our understanding of important viruses. Through high-throughput analysis, genetic manipulation, and its compact genome, yeast provides insights into various aspects related to virus replication.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Sofia F. Reis, Susana Messias, Rita Bastos, Vitor J. Martins, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Manuel A. Coimbra, Elisabete Coelho
Summary: Brewing practice involves reusing the same yeast for subsequent fermentations. The yeast used for Lager beer (Saccharomyces pastorianus) is widely reused without changes in fermentation performance. However, the yeast used for Ale beer (Saccharomyces cerevisiae) is either partially or not reused due to poor performance. The study found that cells modulate their wall polysaccharides to increase cell wall strength.
CARBOHYDRATE POLYMERS
(2023)
Article
Plant Sciences
Bruna B. F. Cal, Luana B. N. Araujo, Brenno M. Nunes, Claudia R. da Silva, Marcia B. N. Oliveira, Bianka O. Soares, Alvaro A. C. Leitao, Marcelo de Padula, Debora Nascimento, Douglas S. A. Chaves, Rachel F. Gagliardi, Flavio J. S. Dantas
Summary: The ethanolic extract of Petiveria alliacea L. showed cytotoxic and mutagenic effects on Saccharomyces cerevisiae, possibly mediated by oxidative lesions.
Article
Biotechnology & Applied Microbiology
Lauriane Cacheux, Loic Ponger, Michele Gerbault-Seureau, Florence Anne Richard, Christophe Escude
Article
Environmental Sciences
Qin Qiao, Severine Le Manach, Helene Huet, Evelyne Duvernois-Berthet, Soraya Chaouch, Charlotte Duval, Benoit Sotton, Loic Ponger, Arul Marie, Lucrece Matheron, Sarah Lennon, Gerard Bolbach, Chakib Djediat, Cecile Bernard, Marc Edery, Benjamin Marie
ENVIRONMENTAL POLLUTION
(2016)
Article
Multidisciplinary Sciences
Qin Qiao, Severine Le Manach, Benoit Sotton, Helene Huet, Evelyne Duvernois-Berthet, Alain Paris, Charlotte Duval, Loic Ponger, Arul Marie, Alain Blond, Lucrece Matheron, Joelle Vinh, Gerard Bolbach, Chakib Djediat, Cecile Bernard, Marc Edery, Benjamin Marie
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Alexandre Ceccaldi, Arumugam Rajavelu, Christine Champion, Christine Rampon, Renata Jurkowska, Gytis Jankevicius, Catherine Senamaud-Beaufort, Loic Ponger, Nathalie Gagey, Hana Dali Ali, Joerg Tost, Sophie Vriz, Sindu Ros, Daniel Dauzonne, Albert Jeltsch, Dominique Guianvarc'h, Paola B. Arimondo
Article
Biochemistry & Molecular Biology
Jean-Claude Walser, Loic Ponger, Anthony V. Furano
Article
Obstetrics & Gynecology
Ewka C. M. Nelissen, John C. M. Dumoulin, Florence Busato, Loic Ponger, Lars M. Eijssen, Johannes L. H. Evers, Joerg Tost, Aafke P. A. van Montfoort
HUMAN REPRODUCTION
(2014)
Article
Multidisciplinary Sciences
Christine Champion, Dominique Guianvarc'h, Catherine Senamaud-Beaufort, Renata Z. Jurkowska, Albert Jeltsch, Loic Ponger, Paola B. Arimondo, Anne-Laure Guieysse-Peugeot
Article
Genetics & Heredity
Audrey Miroglio, Helene Jammes, Joerg Tost, Loic Ponger, Ivo Glynne Gut, Hafida El Abdalaoui, Joel Coste, Stanislas Chaussade, Paola B. Arimondo, Dominique Lamarque, Luisa Dandolo
Article
Biochemical Research Methods
Carene Rizzon, Loic Ponger, Brandon S. Gaut
PLOS COMPUTATIONAL BIOLOGY
(2006)
Article
Biochemistry & Molecular Biology
L Ponger, WH Li
MOLECULAR BIOLOGY AND EVOLUTION
(2005)
Article
Biochemical Research Methods
L Ponger, D Mouchiroud
Article
Biochemistry & Molecular Biology
L Ponger, L Duret, D Mouchiroud
Letter
Biochemistry & Molecular Biology
M Robinson-Rechavi, L Ponger, D Mouchiroud
MOLECULAR BIOLOGY AND EVOLUTION
(2000)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)