Article
Multidisciplinary Sciences
Kobe Janssen, Filip Claes, Dido Van de Velde, Vanessa L. Wehbi, Bert Houben, Yulia Lampi, Mieke Nys, Laleh Khodaparast, Ladan Khodaparast, Nikolaos Louros, Rob van der Kant, Joffre Verniers, Teresa Garcia, Meine Ramakers, Katerina Konstantoulea, Katerina Maragkou, Ramon Duran-Romana, Monica Musteanu, Mariano Barbacid, Bernard Scorneaux, Els Beirnaert, Joost Schymkowitz, Frederic Rousseau
Summary: Mutant KRAS is a challenging target for classical small molecule drugs, but this study shows that its misfolding can be induced by synthetic peptides derived from specific regions of the oncoprotein. These peptides were able to inhibit the function of mutant KRAS and showed antiproliferative activity against cancer cells. The findings provide proof-of-concept for exploiting the intrinsic misfolding propensity of KRAS as a therapeutic strategy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Wenbo Zhang, Mingwei Liu, Lanlan Yu, Shanshan Mo, Zhun Deng, Shuli Liu, Yanlian Yang, Chen Wang, Chenxuan Wang
Summary: This study demonstrates the contribution of various noncovalent interactions to supramolecular assembly by investigating the self-assembly of peptides.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Multidisciplinary Sciences
Kimberly Jia Yi Low, Anandalakshmi Venkatraman, Jodhbir S. Mehta, Konstantin Pervushin
Summary: Protein aggregation and deposition in the form of amyloid fibrils is a characteristic feature of amyloid diseases. While most research focuses on inhibiting amyloid formation, it is equally important to understand the disaggregation mechanisms and their potential therapeutic applications. This review compiles the known mechanisms of amyloid disaggregation and explores the use of disaggregases for treating amyloidosis.
JOURNAL OF ADVANCED RESEARCH
(2022)
Review
Food Science & Technology
Jiaojiao Zhou, Jilai Jia, Jiangling He, Jinjie Li, Jie Cai
Summary: Food safety issues pose a significant threat to public health and require accurate, efficient, sensitive, and economical detection methods. Cyclodextrins (CDs), with their non-toxic and non-caloric properties, have shown promise in enhancing stability and selectively capturing target molecules for improved food detection sensitivity.
Review
Biochemistry & Molecular Biology
Azadeh Beygmoradi, Ahmad Homaei, Roohullah Hemmati, Pedro Fernandes
Summary: Protein folding is a process that determines the three-dimensional structure of a protein. Recombinant protein production can face problems like proteolysis, incorrect folding, and protein aggregation. Identifying proper protein refolding conditions is challenging, and molecular chaperones can help in proper folding. Proper protein folding is essential for post-translational modifications, and incorrect folding can lead to cellular damage. Understanding recombinant protein expression and addressing folding issues are important.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Alexey S. Morgunov, Kadi L. Saar, Michele Vendruscolo, Tuomas P. J. Knowles
Summary: Protein function relies on inter-molecular interactions occurring between proteins in various conformational states and levels of disorder, forming structures that can range in size and driving biological processes. Understanding the parameters, formation process, function, and potential disease-causing effects of these interactions are core questions in molecular biosciences. Recent advances in machine learning methods for solving the protein folding problem present new challenges and opportunities for the next frontiers in biomolecular science.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Shan He, Xinyue He, Lei Liu, Wenbo Zhang, Lanlan Yu, Zhun Deng, Feiyi Zhang, Mo Shanshan, Yue Fan, Zhao Xinyue, Lun Wang, Wang Chenxuan, Zhang Shuyang
Summary: Substantial controversies exist in the exploration of the molecular mechanism of heart failure, with recent research suggesting that cardiac transthyretin (TTR) amyloidosis is a major underdiagnosed cause. Investigating and modulating TTR misfolding and amyloidal aggregation can provide new insights into the molecular mechanisms of heart failure and offer novel treatment possibilities.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Alice Lippi, Anita Krisko
Summary: Protein quality control mechanisms are involved in various stages of protein lifetime. Protein aggregates, formed when proteins lose their solubility, play a role in aging and protein folding diseases, but may also serve as an adaptive mechanism for stress survival. This review discusses the different roles of protein aggregation and their potential implications in stress, aging, and disease.
JOURNAL OF NEUROCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Liisa Lutter, Liam D. Aubrey, Wei-Feng Xue
Summary: Predicting the highly ordered three-dimensional structures of amyloid protein fibrils from their monomeric self-assembly precursors remains a challenging aspect of the classical protein folding problem, due to the polymorphic nature of amyloid assembly. Understanding the diversity and individuality of amyloid structures, as well as the link to biology and disease phenotypes, is essential. Current research focuses on resolving the structural basis and biological consequences of polymorphic amyloid assemblies using various techniques such as cryo-EM, ssNMR, and AFM.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Nanoscience & Nanotechnology
Nam Hyeong Kim, Hojae Choi, Zafar Muhammad Shahzad, Heesoo Ki, Jaekyoung Lee, Heeyeop Chae, Yong Ho Kim
Summary: Proteins play a crucial role in various phenomena throughout the life of living things. They form complex structures and regulate reactions, as well as exhibit different biological functions depending on the situation. The self-assembly of proteins is the fundamental principle behind the formation of hierarchical structures. These structures enable multivalent binding, ultra-sensitive regulation, and compartmentalization, and have been extensively utilized in biology. Recent advancements in the de novo design of protein assemblies have shown promising applications in understanding biological phenomena and therapeutics.
Review
Pharmacology & Pharmacy
Karen Escobar, Karla A. Garrido-Miranda, Ruth Pulido, Nelson Naveas, Miguel Manso-Silvan, Jacobo Hernandez-Montelongo
Summary: This review comprehensively covers the chemical properties and synthesis routes of CD/CTR cross-linking, as well as their applications as drug-delivery systems on different substrates. The study found that the CD/CTR biopolymers were nontoxic, environmentally friendly, and inexpensive, making them widely used in coating implants and biomedical materials. The importance of pretreatment methods on substrates and the analysis of release mechanisms, including the Korsmeyer-Peppas mathematical model, were also discussed. The flexibility of CD/CTR in hosting a wide variety of drugs, integrating with diverse implantable materials, and controllable release kinetics provide a range of advantages for future uses.
Article
Biochemistry & Molecular Biology
Filipe E. P. Rodrigues, Antonio J. Figueira, Claudio M. Gomes, Miguel Machuqueiro
Summary: Computational analysis revealed that Aβ-Lys28 plays a key role in stabilizing interactions with S100B, involving residues such as Met79, Thr82, and Glu86. Coulombic interactions, potentially involving the Lys28(Aβ)/Glu86(S100B) pair, are crucial for the holdase-type chaperone activity of S100B, and high ionic strength can reduce the anti-aggregation activity of the Aβ-S100B interaction through electrostatic perturbation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Wei Yang, Xiaoqing Qu, Chujun Deng, Lei Dai, Haoyu Zhou, Guihua Xu, Bo Li, Nazarenko Yulia, Changzhong Liu
Summary: This study investigated the synergistic enhancement in the thermal co-aggregation and gelation of lactoferrin and alpha-lactalbumin under specific conditions. It found that there is a strong rheological synergism between the two proteins, leading to reduced gelation times. The research enhances understanding of heat sensitive protein-heat stable protein thermal aggregation and gelation processes.
FOOD RESEARCH INTERNATIONAL
(2021)
Review
Chemistry, Multidisciplinary
Surabhi Jaiswal, Amit Manhas, Alok Kumar Pandey, Smriti Priya, Sandeep K. Sharma
Summary: Engineered nanoparticles interact with proteins, potentially leading to changes in protein structure and function, with implications for the therapeutic potential in protein misfolding diseases.
Article
Biochemistry & Molecular Biology
Caroline Haikal, Lei Ortigosa-Pascual, Zahra Najarzadeh, Katja Bernfur, Alexander Svanbergsson, Daniel E. Otzen, Sara Linse, Jia-Yi Li
Summary: Research has shown that different PSM alpha peptides accelerate the aggregation of alpha-synuclein and increase ThT fluorescence while shortening the lag phase. The effects of PSM alpha peptides on the kinetics of alpha-synuclein aggregation vary, which could have implications for the prevention of potential neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Andy Chevigne, Vincenzo Campizi, Martyna Szpakowska, David Bourry, Marie-Eve Dumez, Jose C. Martins, Andre Matagne, Moreno Galleni, Alain Jacquet
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Andre Matagne, Laetitia Bolle, Rachida El Mahyaoui, Danielle Baeyens-Volant, Mohamed Azarkan
Article
Microbiology
Alessandra Piccirilli, Paola Sandra Mercuri, Moreno Galleni, Massimiliano Aschi, Andre Matagne, Gianfranco Amicosante, Mariagrazia Perilli
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Biochemistry & Molecular Biology
Ismahene Dahmane, Caroline Montagner, Andre Matagne, Shrinivas Dumbre, Piet Herdewijn, Mohammed Terrak
Editorial Material
Biophysics
Mauro Dalla Serra, Robert J. C. Gilbert, Andre Matagne, Patrick England
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2018)
Article
Spectroscopy
Guillaume Roussel, Yves Caudano, Andre Matagne, Mark S. Sansom, Eric A. Perpete, Catherine Michaux
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2018)
Article
Multidisciplinary Sciences
Mohamed Azarkan, Georges Feller, Julie Vandenameele, Raphael Herman, Rachida El Mahyaoui, Eric Sauvage, Arnaud Vanden Broeck, Andre Matagne, Paulette Charlier, Frederic Kerff
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Aysegul Turupcu, Alice M. Bowen, Alexandre Di Paolo, Andre Matagne, Chris Oostenbrink, Christina Redfield, Lorna J. Smith
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2020)
Article
Chemistry, Analytical
Emeline Hanozin, Elodie Grifnee, Hugo Gattuso, Andre Matagne, Denis Morsa, Edwin De Pauw
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Maximilien Lopes-Rodrigues, Andre Matagne, David Zanuy, Carlos Aleman, Eric A. Perpete, Catherine Michaux
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2020)
Article
Chemistry, Physical
Alessandra Piccirilli, Fabrizia Brisdelli, Jean Denis Docquier, Massimiliano Aschi, Sabrina Cherubini, Filomena De Luca, Andre Matagne, Gianfranco Amicosante, Mariagrazia Perilli
Article
Biophysics
Ruth Kellner, Romain Malempre, Julie Vandenameele, Alain Brans, Anne-Francoise Hennen, Noemie Rochus, Alexandre Di Paolo, Marylene Vandevenne, Andre Matagne
Summary: The stability and activity of proteins are significantly affected by various factors, with proper buffer and pH conditions playing a crucial role. In this study, a high throughput screen of 158 different buffers/pH conditions was developed to evaluate their impact on protein stability and function. This approach can provide valuable insights for academic and industrial applications.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Megane A. Poncin, Pierre Van Meerbeeck, Joshua D. Simpson, Andre Clippe, Francois Tyckaert, Fabrice Bouillenne, Herve Degand, Andre Matagne, Pierre Morsomme, Bernard Knoops, David Alsteens
Summary: Human peroxiredoxin-5 (PRDX5) plays a dual role in brain ischemia-reperfusion injury by acting as an antioxidant enzyme intracellularly, but exacerbating neural cell death extracellularly. The redox state of PRDX5 cysteine residues plays a crucial role in TLR4-induced inflammation, with disulfide PRDX5 showing higher binding propensity and IL-8 secretion compared to mutant forms.
Article
Multidisciplinary Sciences
Simona De Franco, Julie Vandenameele, Alain Brans, Olivier Verlaine, Katerina Bendak, Christian Damblon, Andre Matagne, David J. Segal, Moreno Galleni, Joel P. Mackay, Marylene Vandevenne
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Analytical
Joelle De Meutter, Julie Vandenameele, Andre Matagne, Erik Goormaghtigh
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)