期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1804, 期 9, 页码 1780-1784出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2010.05.004
关键词
Leucine-rich repeat kinase 2; N-terminal domain; Ankyrin domain; Expression and purification; Oligomerization
资金
- Chinese Ministry of Science and Technology [2006AA02Z173]
Leucine-rich repeat kinase 2 gene is a key factor for Parkinson's disease and encodes for a large protein kinase LRRK2 (280 kDa) with multiple domains, including the different repeat sequences at the N-terminus such as ankyrin domain. Here, we successfully expressed and purified two kinds of LRRK2's N-terminal fragments N1 (aa12-320) and N2 (aa12-860). The purified N2 protein was identified by mass spectrometry and N1's molecular weight was determined to be 33.23 kDa. Gel filtration revealed that N1 exhibits as monomer, dimer and tetramer and N2 as oligomer in solution. N1's multiple oligomeric states were further proved by native-page and cross-linking gel experiments. Circular dichroism spectrum indicated that N1 and N2 contain both a helixes and 13 sheets. The polymerization character of LRRK2 N-terminal region would be speculated to relate with its biological function. (C) 2010 Elsevier BM. All rights reserved.
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