Article
Biochemistry & Molecular Biology
Khadijah A. Altammar, Jonathan Guyang Ling, Hussein M. Al-Bajalan, Iuan-Sheau Chin, Mukram Mohamed Mackeen, Nor Muhammad Mahadi, Abdul Munir Abdul Murad, Farah Diba Abu Bakar
Summary: Cutinases are hydrolytic enzymes that can degrade plant cutin. A new cutinase homolog, AnCUT3, has been identified and characterized in this study. The recombinant AnCUT3 exhibited optimal temperature and pH, as well as high catalytic efficiency. It also showed potential for degrading and modifying the surface of certain polymers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Food Science & Technology
Xue Zhang, Chunyue Zhang, Mian Zhou, Quanming Xia, Liqiang Fan, Liming Zhao
Summary: Chitin and its derivatives have diverse applications, and this research achieved increased chitin content in yeast cell walls through gene overexpression and culture condition optimization. This is the first report of chitin production in engineered Pichia pastoris via biosynthetic pathway enhancement.
Article
Engineering, Environmental
Minjie Gao, Yuchen Zhou, Jiajun Yan, Li Zhu, Zhitao Li, Xiuyu Hu, Xiaobei Zhan
Summary: Rhodium is widely used in catalysis, but its adsorption by microorganisms is not well studied. In this work, waste P. pastoris and recombinant P. pastoris GS-R were used as novel adsorbents for Rh(III) adsorption, showing high adsorption capacity and selectivity. The study developed an environmentally-friendly strategy for recovering Rh(III) and other precious metals using P. pastoris.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Immunology
Nicholas J. MacDonald, Kavita Singh, Karine Reiter, Nguyen Vu, Richard Shimp, Apostolos G. Gittis, Beth Chen, Martin Burkhardt, Baoshan Zhang, Zhixiong Wang, Raul Herrera, Mackenzie Moler, Duck-Yeon Lee, Sachy Orr-Gonzalez, Jessica Herrod, Lynn E. Lambert, Kelly M. Rausch, Olga Muratova, David S. Jones, Yimin Wu, Albert J. Jin, David N. Garboczi, Patrick E. Duffy, David L. Narum
Summary: The development of a malaria vaccine that can block transmission of the parasite from mosquitoes to humans is crucial for malaria elimination efforts. The use of Pfs25, a protein on the surface of the parasite, is being investigated as a transmission-blocking vaccine. Two recombinant forms of Pfs25, Pfs25H and Pfs25M, have been studied for their structural and immunological differences.
Article
Fisheries
Zixuan Wu, Yujie Liu, Jiaqi Zheng, Yongzhao Zhou, Kefan Xing, Yuying Sun, Jiquan Zhang
Summary: Arginine kinase is crucial for organisms with high energy demands, and in Exopalaemon carinicauda, it may play a similar role as in other crustaceans. Its expression is predominantly in muscle and can change in response to bacterial challenges. Recombinant expression and purification of EcAK showed similar enzymatic characteristics to AK from Euphausia superba.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Engineering, Biomedical
Shuo-Fu Yuan, Sierra M. Brooks, Annalee W. Nguyen, Wen-Ling Lin, Trevor G. Johnston, Jennifer A. Maynard, Alshakim Nelson, Hal S. Alper
Summary: Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity, which is not easily portable or reusable for on-demand protein production applications. In this study, a hydrogel-based technique was used to immobilize engineered Pichia pastoris for preservable, on-demand production and secretion of medium- and high-molecular weight proteins. This platform shows potential for establishing a cost-effective bioprocessing strategy for on-demand protein production.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Kristina Kantorova, Martin Simkovic, Albert Breier, Martin Rebros
Summary: The study successfully upscaled the production of Myrosinase to a 3 L laboratory fermenter, optimizing fermentation conditions resulted in a threefold increase in Myrosinase productivity and 1.5-fold increase in dry cell weight.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yuanke Guo, Yang Liao, Jing Wang, Chen Ma, Jialun Qin, Jiao Feng, Yan Li, Xin Wang, Kequan Chen
Summary: Methanol, a potential substitute for sugar-based raw materials in biomanufacturing, has gained significant attention. This review focuses on the application of Pichia pastoris, a methylotrophic cell factory, in methanol-based biomanufacturing. The article discusses the current understanding of methanol metabolism in P. pastoris, important factors influencing its methanol utilization ability, recent advances in using engineered P. pastoris for producing various chemicals from methanol, as well as future challenges and potential solutions in developing P. pastoris as an efficient cell factory for methanol-based biomanufacturing.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Roberto Carlos Aristicas Ribalta, Lisandra Martinez Valdes, Meinardo Lafargue Gamez, Sonia Rodriguez Davydenko, Eric Dubreucq, Veronique Perrier, Benoit Moreau, Reinaldo Fraga Vidal
Summary: This study reveals the potential application of a novel dextranase variant in the sugar industry, which demonstrates advantages in both production yield and efficiency. It also provides evidence of carbohydrate-protein interactions.
APPLIED SCIENCES-BASEL
(2022)
Article
Fisheries
Dapeng Yu, Tingting Weng, Guanjian Yang, Hongli Xia, Zhen Gan, Zhiwen Wang, Yuan Li, Liqun Xia, Kevin W. H. Kwok, Jianlin Chen, Yishan Lu
Summary: This study characterized Nklysin from grouper and investigated its expression pattern, biological function in bacterial infection, and role in viral replication. The findings suggest that grouper Nklysin may be a potential agent for antibacterial and antiviral infection in the future.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Josephine Nocillado, Peter Palma, Tianfang Wang, Evelyn Grace de Jesus-Ayson, Berta Levavi-Sivan, Abigail Elizur
Summary: This study demonstrates the potential of using yeast as a vehicle to orally deliver recombinant gonadotropin-releasing hormone (rGnRH1) for stimulating gonadal development in juvenile fish. The results show that the recombinant GnRH1 expressed in yeast exhibits both in vitro and in vivo biological activity. This technology has the potential to provide a non-invasive method for managing the reproduction of large, late-maturing fish species and delicate endangered species.
Article
Biochemical Research Methods
Neil C. Dalvie, Timothy Lorgeree, Andrew M. Biedermann, Kerry R. Love, J. Christopher Love
Summary: This study developed a knockout fragment to repair Cas9-induced gene disruptions and identified knockout clones with high fidelity via PCR, demonstrating improved genomic stability.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Neil C. Dalvie, Sergio A. Rodriguez-Aponte, Brittany L. Hartwell, Lisa H. Tostanoski, Andrew M. Biedermann, Laura E. Crowell, Kawaljit Kaur, Ozan S. Kumru, Lauren Carter, Jingyou Yu, Aiquan Chang, Katherine McMahan, Thomas Courant, Celia Lebas, Ashley A. Lemnios, Kristen A. Rodrigues, Murillo Silva, Ryan S. Johnston, Christopher A. Naranjo, Mary Kate Tracey, Joseph R. Brady, Charles A. Whittaker, Dongsoo Yun, Natalie Brunette, Jing Yang Wang, Carl Walkey, Brooke Fiala, Swagata Kar, Maciel Porto, Megan Lok, Hanne Andersen, Mark G. Lewis, Kerry R. Love, Danielle L. Camp, Judith Maxwell Silverman, Harry Kleanthous, Sangeeta B. Joshi, David B. Volkin, Patrice M. Dubois, Nicolas Collin, Neil P. King, Dan H. Barouch, Darrell J. Irvine, J. Christopher Love
Summary: Subunit vaccines based on recombinant proteins are crucial tools for controlling COVID-19 due to their low manufacturing cost, scalability, and proven safety and efficacy. Engineered RBD variants exhibit high manufacturability and immunogenicity, showing enhanced immune responses after a single dose and cross-reactivity to new variants of concern.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Hewen Deng, Mengjiao Li, Qiuyang Zhang, Chencheng Gao, Zhanyun Song, Chunhua Chen, Zhuo Wang, Xin Feng
Summary: Bacillary White Diarrhea, caused by Salmonella pullorum, is a severe disease that leads to high mortality in chicks. Antibiotic resistance has made treatment challenging, so a Salmonella bacteriophage endolysin, LySP2, was explored as a potential treatment option in this study.
Article
Biotechnology & Applied Microbiology
Dominic Gaetjen, Florian Tomszak, Johann-Christoph Dettmann, Miriam Droste, Volker Noelle, Marek Wieczorek
Summary: This study successfully established a Pichia pastoris surface display system for antibody selection and expression. By optimizing the combination of genetic elements, high levels of antibody fragment surface display can be achieved.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Claudia Yanez-Neco, Fadia Cervantes, Lorena Amaya-Delgado, Antonio O. Ballesteros, Francisco J. Plou, Javier Arrizon
Summary: This study demonstrated the synthesis of GOS using B-gal42 from Pantoea anthophila, overexpressed in E. coli, with lactose or milk whey as substrates. Both milk whey and pure lactose resulted in similar GOS yields, showing the potential of using B-gal42 for added-value product production from whey.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Maria Molina-Gutierrez, Leonor Rodriguez-Sanchez, Carmen Donoro, M. Jesus Martinez, Alicia Prieto
Summary: The recombinant lipase of Ophiostoma piceae immobilized on magnetic nanoparticles shows significant sterol esterase activity, capable of efficiently catalyzing the production of stanol esters under solvent-free conditions. Through a two-step cascade reaction, fatty acid methyl esters synthesized from cooking oil wastes can be used for stanol transesterification, achieving high conversion rates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Javier Rodriguez-Salarichs, Mario Garcia de Lacoba, Alicia Prieto, Maria Jesus Martinez, Jorge Barriuso
Summary: The study reveals that certain lipases from the Candida rugosa-like family exhibit wide substrate specificity on both triglycerides and sterol esters. By analyzing the full catalytic mechanisms of these enzymes, insights into their mechanistic properties have been gained. A computational protocol was developed to predict substrate specificity, using C. rugosa and O. piceae lipases as model enzymes and different triglycerides and cholesterol esters as model substrates, and the protocol was validated by comparing results with existing literature, offering a valuable tool for virtual screening of substrates for enzymes of the C. rugosa-like family with unknown catalytic properties.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Biochemistry & Molecular Biology
Juan Antonio Mendez-Liter, Ivan Ayuso-Fernandez, Florian Csarman, Laura Isabel de Eugenio, Noa Miguez, Francisco J. Plou, Alicia Prieto, Roland Ludwig, Maria Jesus Martinez
Summary: The first lytic polysaccharide monooxygenase (LPMO) from the ascomycete Talaromyces amestolkiae has been successfully expressed and characterized. Analyses showed C1/C4 oxidation regioselectivity and confirmed the enzyme's thermostability, with implications for saccharification applications in cellulosic and lignocellulosic materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alejandro Gonzalez-Benjumea, Dolores Linde, Juan Carro, Rene Ullrich, Martin Hofrichter, Angel T. Martinez, Ana Gutierrez
Summary: Research on epoxide metabolites from n-3 and n-6 polyunsaturated fatty acids has shown that fungal unspecific peroxygenases can convert these fatty acids into mono-epoxides with high regio- and enantioselectivity. Specifically, Agrocybe aegerita and Collariella virescens enzymes demonstrated 91% conversion rates with strict regioselectivity. This study highlights the potential synthetic utility of fungal peroxygenases in fatty acid epoxidation.
Article
Biochemistry & Molecular Biology
Andres Olmedo, Rene Ullrich, Martin Hofrichter, Jose C. del Rio, Angel T. Martinez, Ana Gutierrez
Summary: Unspecific peroxygenases (UPOs) are extracellular enzymes capable of oxygenating various aliphatic and aromatic substrates using peroxide. Recent study shows that rCciUPO and AaeUPO can shorten the chain length of both saturated and unsaturated fatty acids in a different manner compared to other UPOs.
Article
Biochemistry & Molecular Biology
Dolores Linde, Elena Santillana, Elena Fernandez-Fueyo, Alejandro Gonzalez-Benjumea, Juan Carro, Ana Gutierrez, Angel T. Martinez, Antonio Romero
Summary: This study successfully crystallized two UPO enzymes, rMroUPO and rCviUPO, from different fungal groups through sequence optimization and Escherichia coli expression, revealing their crystal structures and dimeric protein characteristics.
Article
Biochemistry & Molecular Biology
Dolores Linde, Alejandro Gonzalez-Benjumea, Carmen Aranda, Juan Carro, Ana Gutierrez, Angel T. Martinez
Summary: Vegetable oils are valuable renewable resources for the production of bio-based chemicals and intermediates. Enzymes can be used as environmentally friendly alternatives to chemical catalysis in the epoxidation reactions of vegetable oils. By engineering the active site of the enzyme and optimizing the process, the epoxidation yield can be significantly enhanced.
Article
Biochemistry & Molecular Biology
Esteban D. Babot, Carmen Aranda, Jan Kiebist, Katrin Scheibner, Rene Ullrich, Martin Hofrichter, Angel T. Martinez, Ana Gutierrez
Summary: Terminal alkenes are attractive starting materials for synthesizing epoxides, which are crucial intermediate building blocks in various industries. Researchers have explored the use of unspecific peroxygenases (UPOs) as biocatalysts for selectively epoxidizing terminal alkenes. Several UPOs have demonstrated the ability to catalyze the epoxidation of long-chain terminal alkenes with different selectivities and turnover rates. This approach offers a green and potential alternative to existing synthesis technologies.
Article
Biochemistry & Molecular Biology
Juan A. Mendez-Liter, Ana Pozo-Rodriguez, Enrique Madruga, Maria Rubert, Andres G. Santana, Laura de Eugenio, Cristina Sanchez, Ana Martinez, Alicia Prieto, Maria Jesus Martinez
Summary: This study successfully synthesized three novel EGCG glycosides using two mutagenic variants enzymes and optimized reaction conditions. Glycosylation increased the solubility and thermal stability of EGCG. All three glycosides showed stronger inhibition on cancer cells, while glucosylated and sophorylated derivatives showed better neuroprotective effects.
Article
Biochemistry & Molecular Biology
Ana Pozo-Rodriguez, Juan A. A. Mendez-Liter, Rocio Garcia-Villalba, David Beltran, Eva Calvino, Andres G. Santana, Laura I. I. De Eugenio, Francisco Javier Canada, Alicia Prieto, Jorge Barriuso, Francisco A. A. Tomas-Barberan, Maria Jesus Martinez
Summary: Resveratrol was glycosylated by using rXynSOS-E236G glycosynthase, resulting in the production of 3-O-beta-D-xylobiosyl resveratrol as the major product. Response surface methodology was used to optimize the reaction, yielding 35% of 3-O-beta-D-xylobiosyl resveratrol. Xylobiosylation decreased the antioxidant capacity of resveratrol, but significantly improved its solubility for improved delivery and transit to the colon.
Article
Biotechnology & Applied Microbiology
Patricia Ferreira, Juan Carro, Beatriz Balcells, Angel T. Martinez, Ana Serrano
Summary: In this study, the quinone reductase activity in three AAO flavooxidases was analyzed, with O-2 as the preferred oxidizing substrate. The results suggest that the aryl-alcohol dehydrogenase activity of these enzymes, although less significant than their oxidase activity, may play a physiological role in fungal decay of lignocellulose by preventing repolymerization through the reduction of quinones. These findings expand the understanding of the role of AAO in lignin biodegradation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Polymer Science
David Rodriguez-Escribano, Felipe de Salas, Rocio Pliego, Gisela Marques, Thomas Levee, Anu Suonpaa, Ana Gutierrez, angel T. Martinez, Petri Ihalainen, Jorge Rencoret, Susana Camarero
Summary: Lignins released in black liquors from kraft pulp mills are a valuable but underutilized source of aromatics. Enzymatic depolymerization of kraft lignins using alkaliphilic fungal laccases has been made possible by our recent enzyme-directed evolution approach, which generated laccases with high activity and stability at pH 10. Our study demonstrates the ability of these tailor-made laccases to oxidize, demethylate, and depolymerize eucalyptus kraft lignin at pH 10. However, in high enzyme and lignin concentrations, repolymerization of the oxidized products becomes dominant. To overcome this, we successfully conducted the enzymatic reaction in a bench-scale reactor coupled to a membrane separation system.
Article
Food Science & Technology
Fadia Cervantes, David Fernandez-Polo, Zoran Merdzo, Noa Miguez, Martin Garcia-Gonzalez, Antonio O. Ballesteros, Maria Fernandez-Lobato, Francisco J. Plou
Summary: During the synthesis of prebiotic carbohydrates, it is desirable to remove D-glucose as a side-product. This study investigated the use of immobilized yeast for the elimination of D-glucose and D-fructose in sugar syrups with successful results.
ACS FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Pozo-Rodriguez, Juan A. Mendez-Liter, Laura I. de Eugenio, Manuel Nieto-Dominguez, Eva Calvino, Francisco Javier Canada, Andres G. Santana, Jaime Diez, Juan L. Asensio, Jorge Barriuso, Alicia Prieto, Maria Jesus Martinez
Summary: The study explores the use of endoxylanases and their glycosynthase variants in the valorization of hemicellulosic residues and the synthesis of glycosides with improved properties. The results demonstrate the high catalytic efficiency and substrate versatility of these enzymes, particularly in the degradation of beechwood xylan and wheat arabinoxylan. The glycosynthase variant shows enhanced capacity in the synthesis of xylooligosaccharides and glycosylation of phenolic compounds with antioxidant properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)