Article
Biochemistry & Molecular Biology
Zachary Ditzig, Caleb M. Wilson, Jesse Salas, Kinta M. Serve
Summary: This study confirms that mesothelial cells can bind and activate Plg at the cell surface, and this activation plays a crucial role in cell invasion. Additionally, three Plg receptors (alpha-Enolase, Annexin A2, and Plg-R-KT) are identified on the mesothelial cell surface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Christine Lodberg Hvas, Julie Brogaard Larsen
Summary: The fibrinolytic system plays a crucial role in maintaining the haemostatic balance, but accurate diagnosis and effective treatment of fibrinolytic disorders remain challenging in clinical practice, indicating a need for further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Alamelu G. Bharadwaj, Emma Kempster, David M. Waisman
Summary: The generation of the serine protease plasmin is initiated by the binding of its zymogenic precursor, plasminogen, to cell surface receptors. This process plays a crucial role in several physiological processes and cancer cells exploit this mechanism to promote tumor development, progression, and metastasis. The protein S100A10 is identified as a key plasminogen receptor in cancer progression and oncogenesis, with potential as a biomarker for cancer diagnosis and prognosis.
Review
Biochemistry & Molecular Biology
Filomena Napolitano, Nunzia Montuori
Summary: This study discusses the role of platelets in fibrinolysis, highlighting their profibrinolytic properties through the involvement of components in the Plasminogen Activation System (PAS). The findings suggest that while antifibrinolytic effects prevail in the initial phase of hemostasis, platelets may enhance fibrinolysis at later stages through engagement with PAS components. Understanding the spatial and temporal characteristics of platelet-mediated fibrinolysis could lead to improved therapeutic options for bleeding and thrombotic disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Alamelu Bharadwaj, Emma Kempster, David Morton Waisman
Summary: Mutualistic symbiosis refers to a symbiotic relationship between individuals of different species where both benefit. Annexin A2 and S100A10 have a mutualistic symbiosis where their interaction affects the function of both proteins. They rely on each other for certain functions and processes that benefit both.
Article
Biochemistry & Molecular Biology
Oriol Bosch-Sanz, Yvette Rabada, Xevi Biarnes, Javier Pedreno, Luis Caveda, Mercedes Balcells, Jordi Martorell, David Sanchez-Garcia
Summary: Fibrinolysis is a natural process that removes fibrin deposits to ensure blood fluidity. Excessive fibrinolytic activity can cause complications in surgery or trauma. Current antifibrinolytic drugs require high doses and have side effects, limiting their use in patients with renal impairment. This study synthesized a series of compounds and identified a candidate with higher specificity and lower dosage. Molecular docking and dynamics analysis revealed the importance of 1,2,3-triazole in the activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Ashna A. Kumar, Benjamin J. Buckley, Marie Ranson
Summary: This review provides an overview of the role of the urokinase plasminogen activation system (uPAS) in pancreatic cancer, with a focus on pancreatic ductal adenocarcinoma (PDAC), and summarizes the substantial evidence supporting the role of uPAS components in this disease. The review further outlines the potential clinical utility of uPAS components as diagnostic and prognostic biomarkers for PDAC, as well as the rationale for developing novel uPAS-targeted therapeutics.
Article
Oncology
Yunhee Lee, Dongjoon Ko, Junghwa Yoon, Younghoon Lee, Semi Kim
Summary: TMEM52B has been identified as a novel gene expressed widely in normal human tissues. Suppression of TMEM52B in colon cancer cells leads to enhanced epithelial-mesenchymal transition (EMT), invasion, and survival, potentially through multiple signaling pathways. Clinical data suggest that high TMEM52B expression is associated with increased patient survival in various types of cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Dorota Satala, Aneta Bednarek, Andrzej Kozik, Maria Rapala-Kozik, Justyna Karkowska-Kuleta
Summary: The development of infections caused by pathogenic bacteria is closely related to the specific properties of the bacterial cell surface and extracellular hydrolytic activity, as well as the hijacking of host proteolytic cascades by pathogens. This review focuses on the mechanisms of bacterial impact on host plasminogen, including cell surface exposition of various proteins, binding of this host (pro)enzyme and its activators or inhibitors, and the exploitation of fibrinolytic system activation tactics by different bacterial species. The involvement of bacterial factors in modulating plasminogen activation and fibrinolysis during periodontitis is also described.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Qianwen Wang, Chenxiang Qi, Pengxiang Min, Yueyuan Wang, Fengwen Ye, Tianxiang Xia, Yujie Zhang, Jun Du
Summary: By regulating MICAL2, the migratory ability of gastric cancer cells can be influenced, and it involves changes in the E-cadherin/beta-catenin signaling pathway.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Olga Tsaplina, Ekaterina Lomert, Yuliya Berson
Summary: Bacteria use cell surface proteins to interact with host cells, and E-cadherin plays a crucial role in bacterial invasion. Full-length E-cadherin is involved in S. proteamaculans invasion, while both full-length and truncated E-cadherin can promote S. grimesii invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tobias Wijshake, Zhongju Zou, Beibei Chen, Lin Zhong, Guanghua Xiao, Yang Xie, John G. Doench, Lynda Bennett, Beth Levine
Summary: The study revealed previously unrecognized cooperation between Beclin 1 and E-cadherin in mediating tumor suppression in breast cancer cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Hematology
Luiza O. Perucci, Juliana P. Vago, Lindsey A. Miles, Lirlandia P. Sousa
Summary: The Plg/Pla system plays an important role in key steps of inflammation resolution and may be involved in the pathogenesis of inflammatory diseases. Understanding the mechanisms of action of Plg/Pla in inflammation resolution can provide a theoretical basis for the development of new pharmacological interventions and offer new avenues for the treatment of thromboinflammatory conditions.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Oncology
Xiaoyu Zhu, Xiaoping Wang, Yifei Gong, Junlin Deng
Summary: Thyroid carcinoma is a common and problematic cancer, with recurrence and metastasis being significant issues. Understanding the role of EMT and E-cadherin in tumors is crucial for cancer therapeutics.
CANCER CELL INTERNATIONAL
(2021)
Review
Oncology
Manuela Santarosa, Roberta Maestro
Summary: The loss of epithelial integrity and the role of autophagy in cancer progression, particularly in the context of dysregulation of cell adhesion molecules like E-cadherin, are crucial factors. Cell adhesion and EMT play significant roles in the transition process of cancer, while autophagy may have dual effects on tumor progression, acting as both a suppressor and a promoter through mechanisms like EMT and drug resistance.
Letter
Nutrition & Dietetics
Laura Bonfili, Massimiliano Cuccioloni, Chunmei Gong, Valentina Cecarini, Michele Spina, Yadong Zheng, Mauro Angeletti, Anna Maria Eleuteri
CLINICAL NUTRITION
(2022)
Article
Nutrition & Dietetics
Valentina Cecarini, Olee Gogoi, Laura Bonfili, Iolanda Veneruso, Giada Pacinelli, Sara De Carlo, Federica Benvenuti, Valeria D'Argenio, Mauro Angeletti, Nazzareno Cannella, Anna Maria Eleuteri
Summary: A yeast-enriched beer has beneficial effects on Alzheimer's disease by improving cognition, reducing protein aggregation and inflammation, increasing anti-inflammatory cytokines, improving gut bacterial population, and decreasing gut inflammatory fungi.
Article
Clinical Neurology
Oswaldo Lorenzo-Betancor, Livio Galosi, Laura Bonfili, Anna Maria Eleuteri, Valentina Cecarini, Ranieri Verin, Fabrizio Dini, Anna-Rita Attili, Sara Berardi, Lucia Biagini, Patrizia Robino, Maria Cristina Stella, Dora Yearout, Michael O. Dorschner, Debby W. Tsuang, Giacomo Rossi, Cyrus P. Zabetian
Summary: This study described the genetic and pathological findings of three Yellow-crowned parrot siblings with a severe neurodegenerative phenotype. The brains of these parrots exhibited Lewy body-like pathology and a homozygous missense mutation in the CADPS2 gene was identified. Further investigation of this gene may provide important insights into the pathophysiology of Lewy body disorders.
MOVEMENT DISORDERS
(2022)
Article
Genetics & Heredity
Valeria D'Argenio, Iolanda Veneruso, Chunmei Gong, Valentina Cecarini, Laura Bonfili, Anna Maria Eleuteri
Summary: The gut microbiota plays a crucial role in the development and progression of Alzheimer's disease (AD), and understanding the underlying mechanisms can help develop targeted therapies and diagnostic biomarkers. In this study, the gut microbiome and mycobiome of AD mice were characterized to identify specific microbial alterations. The results showed a pro-inflammatory status and AD-related pathogenetic features, suggesting a close connection between bacterial dysbiosis and AD. Further metagenomic studies are needed to explore the mechanisms in detail.
Article
Agriculture, Dairy & Animal Science
Marilena Bazzano, Francesca Arfuso, Laura Bonfili, Anna Maria Eleuteri, Amy McLean, Evelina Serri, Andrea Spaterna, Fulvio Laus
Summary: Working mules often show few signs of illness, so tests and measurements are necessary to detect early signs of disease. This study found that after fieldwork, the electrolyte levels and urea and creatinine concentrations in mules increased, indicating a loss of body water and decreased renal blood flow.
Article
Neurosciences
Chunmei Gong, Laura Bonfili, Yadong Zheng, Valentina Cecarini, Massimiliano Cuccioloni, Mauro Angeletti, Giulia Dematteis, Laura Tapella, Armando A. Genazzani, Dmitry Lim, Anna Maria Eleuteri
Summary: Alzheimer's disease (AD) is characterized by dysfunctions in the ubiquitin-proteasome system (UPS) and autophagy. In this study, researchers obtained immortalized astrocytes from the hippocampus of AD mice and wild-type mice to investigate the proteolytic systems involved. They found downregulation of proteasomal activities and increased autophagy activation in the AD astrocytes. Treatment with 4-phenylbutyric acid partially restored the proteolysis processes. These findings provide further validation of the AD in vitro model and suggest a new mechanism of action for 4-phenylbutyric acid in neurodegenerative disorders.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Valentina Cecarini, Salima Selmi, Massimiliano Cuccioloni, Chunmei Gong, Laura Bonfili, Yadong Zheng, Manuela Cortese, Mauro Angeletti, Soumaya Kilani, Anna Maria Eleuteri
Summary: This study describes the anticancer mechanism of amygdalin in breast cancer cells, involving the modulation of intracellular proteolysis, inhibition of autophagy cascade, and activation of apoptotic events. Amygdalin may be a potential anticancer agent.
Article
Biochemistry & Molecular Biology
Matteo Mozzicafreddo, Devis Benfaremo, Chiara Paolini, Silvia Agarbati, Silvia Svegliati Baroni, Gianluca Moroncini
Summary: This study aimed to find new ligands or new information to design novel effective drugs by performing an initial interaction screening with human intracellular PDGFRa and conducting structural and physicochemical analyses. Several compounds, including Bafetinib, Radotinib, Flumatinib, and Imatinib, showed high affinity for PDGFRa. The structural information obtained from this study could provide useful insights for the development of more effective and targeted treatments for PDGFRa-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Francesca Lombardi, Francesca Rosaria Augello, Paola Palumbo, Laura Bonfili, Serena Artone, Serena Altamura, Jenna Marie Sheldon, Giovanni Latella, Maria Grazia Cifone, Anna Maria Eleuteri, Benedetta Cinque
Summary: This study aimed to evaluate the effect of the lysate from multi-strain probiotic formulation SLAB51 on the HIF pathway in an in vitro model of human intestinal epithelium and its protective role against lipopolysaccharide challenge. The probiotic lysate stabilized HIF-1 alpha through the activation of the PI3K/AKT pathway and the inhibition of NF- kappa B, NOS2, and IL-1 beta increase, thus protecting against LPS-induced inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Daphne Romani, Fabio Marchetti, Corrado Di Nicola, Massimiliano Cuccioloni, Chunmei Gong, Anna Maria Eleuteri, Agustin Galindo, Farzaneh Fadaei-Tirani, Massimo Nabissi, Riccardo Pettinari
Summary: A series of Ga(Q(n))(3) coordination compounds were synthesized and characterized through various techniques. Cytotoxicity studies showed interesting results in terms of selectivity and toxicity values compared with cisplatin. Mechanistic investigations revealed that the gallium(III) complexes induced cancer cell death through ferroptosis, triggering various cell death signals and altering cell redox homeostasis.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Yadong Zheng, Luyan Zhang, Laura Bonfili, Luisa de Vivo, Anna Maria Eleuteri, Michele Bellesi
Summary: Insufficient sleep is a significant public health problem, leading to increased risk of chronic diseases and oxidative damage. Probiotics have antioxidant and anti-inflammatory properties and can counteract oxidative stress and inflammation caused by sleep loss.
Review
Polymer Science
Kun Feng, Lulu Huangfu, Chuanduo Liu, Laura Bonfili, Qisen Xiang, Hong Wu, Yanhong Bai
Summary: Electrospinning and electrospraying are two electrohydrodynamic techniques that have attracted increased interest for stabilizing probiotics. They help improve the viability of probiotics and enhance their delivery in the gastrointestinal tract. This review discusses the classification of electrospinning and electrospraying, their feasibility in constructing probiotic carriers, and their efficacy in stabilizing and delivering probiotics. The current application of electrospun and electrosprayed probiotic formulations is also introduced. Finally, the limitations and future opportunities for using electrohydrodynamic techniques in probiotic stabilization are proposed and analyzed. This work provides a comprehensive understanding of how electrospinning and electrospraying can be used to enhance probiotics, aiding in the development of probiotic therapy and nutrition.
Review
Biochemistry & Molecular Biology
Devis Benfaremo, Silvia Agarbati, Matteo Mozzicafreddo, Chiara Paolini, Silvia Svegliati, Gianluca Moroncini
Summary: Systemic sclerosis, also known as scleroderma or SSc, is a highly varied disease in terms of clinical presentation, disease progression, and treatment response. Recent advancements in skin molecular profiling technologies have enabled the identification of patient subgroups based on gene expression analysis. This narrative review provides an overview of the current status of skin gene expression analysis and emphasizes the benefits of stratifying patients based on their gene signatures. This stratification has the potential to lead to a precision medicine approach in the management of SSc.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)