Article
Microbiology
Wei He, Gangjin Yu, Tianpeng Li, Ling Bai, Yuanyuan Yang, Zixiao Xue, Yonghao Pang, Dana Reichmann, Sebastian Hiller, Lichun He, Maili Liu, Shu Quan
Summary: Our research demonstrates the role of the periplasmic chaperone Spy in maintaining the homeostasis of certain outer membrane proteins (OMPs). Spy utilizes a unique chaperone mechanism to bind OmpX, allowing it to form a partially folded beta-strand secondary structure in a dynamic exchange of conformations. This mechanism differs from that of other E. coli periplasmic chaperones such as Skp and SurA, highlighting the differences in the mechanisms of ATP-independent chaperones.
Article
Microbiology
Nitanshu Garg, Aidan J. Taylor, Federica Pastorelli, Sarah E. Flannery, Phillip J. Jackson, Matthew P. Johnson, David J. Kelly
Summary: The study identified new Cco subunits, a previously uncharacterized set of genes linking copper trafficking and Cco activity, and connections with copper ion homeostasis in this important pathogen, Campylobacter jejuni, causing human food-borne bacterial gastroenteritis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yui Nakata, Yuuto Kitazaki, Hitomi Kanaoka, Erika Shingen, Rina Uehara, Kunihiro Hongo, Yasushi Kawata, Tomohiro Mizobata
Summary: The molecular chaperones HdeA and HdeB help protect client proteins from acid denaturation in E. coli. Both proteins can form fibrils at low pH, but the fibrils can be resolubilized at neutral pH. However, extended incubation at low pH can lead to the formation of irreversible fibrils. The secondary structure of HdeB fibrils remained alpha helical regardless of the conditions under which the fibrils were formed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Rishav Mitra, Varun V. Gadkari, Ben A. Meinen, Carlo P. M. van Mierlo, Brandon T. Ruotolo, James C. A. Bardwell
Summary: ATP-independent chaperone Spy demonstrates substrate-specific behavior when interacting with the complex client apoflavodoxin, tightly binding to partially unfolded states to convert into a pure holdase.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hena Sandhu, Rickard Hedman, Florian Cymer, Renuka Kudva, Nurzian Ismail, Gunnar von Heijne
Summary: The study reveals that the large periplasmic domain of the E. coli inner membrane protease LepB folds post-translationally in vivo, and demonstrates that Force Profile Analysis (FPA) can be utilized to study both co- and post-translational protein folding in the periplasm.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lisa R. Knoke, Jannik Zimmermann, Natalie Lupilov, Jannis F. Schneider, Beyzanur Celebi, Bruce Morgan, Lars I. Leichert
Summary: The thiol redox balance in the periplasm of E. coli was examined using genetically encoded redox probes (roGFP2 and roGFP-iL). The presence of an alternative system for the introduction of disulfide bonds and the role of glutathione in the oxidative folding machinery were explored.
Article
Multidisciplinary Sciences
Anwar Sadat, Satyam Tiwari, S. Sunidhi, Aseem Chaphalkar, Manisha Kochar, Mudassar Ali, Zainab Zaidi, Akanksha Sharma, Kanika Verma, Kannan Boosi Narayana Rao, Manjul Tripathi, Asmita Ghosh, Deepika Gautam, Atul, Arjun Ray, Koyeli Mapa, Kausik Chakraborty
Summary: The surface charge properties of the GroEL/ES chaperonin cavity, especially the negative charges, play a crucial role in its ability to assist protein folding. However, the negative-charge density varies significantly among different bacterial species and is lowest in eukaryotic GroEL/ES homologs. The chaperoning mechanism of GroEL may have changed during evolution to accommodate different mutations on their substrates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Evan T. Powers, Lila M. Gierasch
Summary: The article discusses the protein folding problem within cells and emphasizes the deployment of chaperones and degradation enzymes to minimize the impact of misfolded states. It also highlights the importance of the proteostasis network in solving the proteome folding problem.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler, Robert Speight
Summary: This study presents a thermostable variant of the E. coli AppA phytase, ApV1, with an extra non-consecutive disulfide bond, which shows promising thermostability but leads to decreased production in Pichia pastoris. Co-expression of folding chaperones like protein disulfide bond isomerase (Pdi) significantly increased the production of ApV1 and restored yields to levels comparable with the wild-type enzyme. Engineering for enhanced enzymatic properties may result in folding complexity and reduced production in microbial systems, highlighting the importance of developing improved production strains in parallel to achieve desirable levels of recombinant protein for industrial processes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Eugene Serebryany, Victor Y. Zhao, Kibum Park, Amir Bitran, Sunia A. Trauger, Bogdan Budnik, Eugene I. Shakhnovich
Summary: Non-native conformations are important in protein-misfolding diseases, bioengineering efforts, and molecular evolution. However, there is currently no suitable experimental technique to study them. The authors propose a high-throughput disulfide scanning method to systematically discover and study native and non-native conformations, linking them to molecular, organismal, or evolutionary phenotypes. They applied this approach to the E. coli periplasmic chaperone HdeA and revealed different classes of disordered hydrophobic conformers with varying cytotoxicity.
Article
Multidisciplinary Sciences
Haina Huang, Katrin Karbstein
Summary: This passage discusses the tendency of three-helix junctions to misfold during the assembly of small ribosomal subunits in cells, and reveals the pivotal role of ribosome assembly factors in facilitating their correct folding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Jakub Macosek, Guillaume Mas, Sebastian Hiller
Summary: Molecular chaperones play a key role in bacterial protein homeostasis by facilitating protein folding and providing stability in non-native states.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Physical
Mohd Younus Bhat, Irfan Mir, Laishram Rajendrakumar Singh, Mahboobul Hussain, Tanveer Ali Dar
Summary: Intrinsically disordered proteins (IDPs) play important roles in cellular processes and disease processes, and trehalose affects the structural-functional elasticity of IDPs.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biochemistry & Molecular Biology
Adrian Arrieta, Thomas M. Vondriska
Summary: Maintenance of protein folding homeostasis is crucial for cell survival and cell-specific biological processes. Chromatin organization plays a vital role in cell type specification. Nucleosome organization and histone turnover are key factors in chromatin organization. This review focuses on the criticality of nucleosome proteostasis, the involvement of histone chaperones and ATP-dependent chromatin remodelers, and the disrupted nucleosome proteostasis mechanisms in diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maria Conde-Gimenez, Javier Sancho
Summary: Phenylketonuria is an autosomal recessive disorder caused by PAH variants, and one current therapeutic approach is to use pharmacological chaperones to rescue the enzyme's physiological function. This study investigates the folding equilibrium of PAH to develop new pharmacological chaperones for different forms of the disease. The research shows that both urea and thermal-induced denaturation of PAH result in the accumulation of equilibrium unfolding intermediates, indicating potential targets for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jean-Francois Collet, Seung-Hyun Cho, Bogdan I. Iorga, Camille Goemans
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Editorial Material
Microbiology
Angelika Grundling, Jean-Francois Collet
CURRENT OPINION IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Emile Dupuy, Jean-Francois Collet
Summary: CnoX is a novel protein folding factor that combines holdase chaperone activity with a redox protective function, playing a crucial role under stress conditions to protect proteins from aggregation and irreversible oxidation. It also has a unique ability to cooperate with the GroEL/ES folding machinery, which appears to be conserved during evolution.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Microbiology
Jessica El Rayes, Raquel Rodriguez-Alonso, Jean-Francois Collet
Summary: Bacterial lipoproteins play crucial roles in the cell envelope of Gram-negative bacteria, with recent research focusing on their diverse functions in Escherichia coli and their maturation, sorting, and targeting mechanisms. The identification of lipoproteins on the surface of E. coli and other bacteria has sparked renewed interest in these proteins as potential targets for antibiotic development.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jessica El Rayes, Joanna Szewczyk, Michael Deghelt, Andre Matagne, Bogdan I. Iorga, Seung-Hyun Cho, Jean-Francois Collet, Naemi Csoma
Summary: In Gram-negative bacteria, approximately half of lipoproteins destined for the outer membrane display an intrinsically disordered linker at their N terminus. The unstructured linkers are required for optimal trafficking by the Lol lipoprotein sorting system, re-routing lipoproteins to the inner membrane without them. Replacing the linker with an artificial peptide of similar length and disorder can restore normal outer-membrane targeting in the stress sensor RcsF.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lisa Maier, Camille V. Goemans, Jakob Wirbel, Michael Kuhn, Claudia Eberl, Mihaela Pruteanu, Patrick Muller, Sarela Garcia-Santamarina, Elisabetta Cacace, Boyao Zhang, Cordula Gekeler, Tisya Banerjee, Exene Erin Anderson, Alessio Milanese, Ulrike Lober, Sofia K. Forslund, Kiran Raosaheb Patil, Michael Zimmermann, Barbel Stecher, Georg Zeller, Peer Bork, Athanasios Typas
Summary: The study categorized the activity spectra of antibiotics in gut bacteria, revealing distinct inhibition patterns of different antibiotic classes. The findings suggest potential strategies to protect beneficial bacteria by screening for drugs that counteract antibiotic activity.
Article
Multidisciplinary Sciences
Frederic Sorgeloos, Michael Peeters, Yohei Hayashi, Fabian Borghese, Nicolas Capelli, Melissa Drappier, Teresa Cesaro, Didier Colau, Vincent Stroobant, Didier Vertommen, Gregory de Bodt, Stephane Messe, Ignasi Forne, Felix Mueller-Planitz, Jean-Francois Collet, Thomas Michiels
Summary: Microbes have evolved to exploit cellular kinases called RSKs, which play a role in defending against pathogens. Kaposi's sarcoma-associated herpesvirus and Yersinia use a similar strategy to manipulate RSKs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Emily C. A. Goodall, Georgia L. Isom, Jessica L. Rooke, Karthik Pullela, Christopher Icke, Zihao Yang, Gabriela Boelter, Alun Jones, Isabel Warner, Rochelle Da Costa, Bing Zhang, James Rae, Wee Boon Tan, Matthias Winkle, Antoine Delhaye, Eva Heinz, Jean-Francois Collet, Adam F. Cunningham, Mark A. Blaskovich, Robert G. Parton, Jeff A. Cole, Manuel Banzhaf, Shu-Sin Chng, Waldemar Vollmer, Jack A. Bryant, Ian R. Henderson
Summary: The cell envelope is crucial for the survival of all domains of life, acting as a barrier to the external environment. Disrupting the envelope is a common strategy in antimicrobial treatment, but there are still gaps in our understanding of how different layers of the envelope are coordinated during growth and division. The study identified a gene, yhcB, that plays a key role in envelope stability, cell permeability, and cell size control, highlighting its potential as a new antimicrobial target.
Article
Oncology
Pierre -Florent Petit, Raphaele Bombart, Pierre -Hubert Desimpel, Stefan Naulaerts, Laurie Thouvenel, Jean-Francois Collet, Benoit J. Eynde, Jingjing Zhu
Summary: The delivery of anti-PD-L1 nanobody through tumor-targeting T cells shows promise in overcoming the limitations of immunotherapy based on PD-L1-specific antibodies.
CANCER IMMUNOLOGY RESEARCH
(2022)
Editorial Material
Multidisciplinary Sciences
Michael Deghelt, Jean-Francois Collet
Article
Virology
Bradley Ward, Jean Cyr Yombi, Jean-Luc Balligand, Patrice D. Cani, Jean-Francois Collet, Julien de Greef, Joseph P. Dewulf, Laurent Gatto, Vincent Haufroid, Sebastien Jodogne, Benoit Kabamba, Sebastien Pyr Dit Ruys, Didier Vertommen, Laure Elens, Leila Belkhir
Summary: This paper introduces the HYGIEIA project, which aims to study the molecular mechanisms and post-COVID conditions through a multi-omic approach and network medicine analysis. By analyzing high-throughput sequencing and mass-spectrometry data from multiple biological layers, meaningful discoveries are expected and can be translated into improvements in clinical practice.
Article
Biochemistry & Molecular Biology
Emile Dupuy, Sander Egbert Van der Verren, Jiusheng Lin, Mark Alan Wilson, Alix Vincent Daschsbeck, Felipe Viela, Emmanuell Latour, Alexandr Gennaris, Didier Vertommen, Yves Frederic Dufrene, Bogdan Iuliu Lorga, Camille Veronique Goemans, Han Remaut, Jean-Francois Collet
Summary: Hsp60 chaperonins and their Hsp10 cofactors facilitate protein folding. This study reveals that the bacterial chaperonin GroEL forms a stable complex with the chaperedoxin CnoX, and CnoX likely functions as a redox quality-control plugin for GroEL. The findings suggest that Hsp60 molecular plugins have been conserved through evolution, as proteins sharing structural features with CnoX exist in eukaryotes.
Review
Microbiology
Seung-Hyun Cho, Kilian Dekoninck, Jean-Francois Collet
Summary: This review focuses on the role of two outer membrane sensors, RcsF and NlpE, in bacterial stress response. Two models, LPS perturbation model and BAM assembly model, have been proposed to explain the stress sensing mechanism of RcsF. As for NlpE, its function depends on different domains, with NlpE NTD involved in signaling and NlpE CTD playing a key role in sensing adherence to a hydrophobic surface.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Pierre Godessart, Adelie Lannoy, Marc Dieu, Sander E. Van der Verren, Patrice Soumillion, Jean-Francois Collet, Han Remaut, Patricia Renard, Xavier De Bolle
Summary: In alpha-proteobacteria, the outer membrane is covalently linked to the peptidoglycan through cross-links between beta-barrel-shaped proteins and peptidoglycan, catalyzed by l,d-transpeptidases. The presence of a conserved alanyl-aspartyl motif at the N-terminus of the anchored proteins is crucial for the stability of the outer membrane. This mechanism of attachment may be widespread in alpha-proteobacteria, with potential implications for envelope integrity.
NATURE MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)