Review
Biochemistry & Molecular Biology
Laura L. Thompson, Kailee A. Rutherford, Chloe C. Lepage, Kirk J. McManus
Summary: The SCF complex is a group of E3 ubiquitin ligase complexes that play crucial roles in regulating cellular processes such as gene transcription and the cell cycle. Aberrant expression or function of SCF complex members is associated with various cancer types, highlighting the importance of further characterizing their functions for potential therapeutic development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Junjie Gao, Dandan Yang, Ruoxue Cao, Hua Huang, Jia Ma, Zhiwei Wang, Jun Xia, Xueshan Pan
Summary: Fbxo5, as a substrate recognition subunit of SCF protein, is critically involved in carcinogenesis and could be a potential target for cancer therapy.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Review
Plant Sciences
Jamie N. Orr, Robbie Waugh, Isabelle Colas
Summary: Meiosis is a crucial cell division process for sexual reproduction, where ubiquitination plays a key role in regulating cellular processes, particularly in its localization and function in plant meiosis. Ubiquitination not only mediates protein degradation but also potentially regulates the activation of key transcription factors, playing diverse roles in chromosome segregation, recombination, and synapsis. Components of the ubiquitination cascade in plant meiosis are still not fully understood compared to other processes and eukaryotes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biology
Lev Brio, Danit Wasserman, Efrat Michaely-Barbiro, Gal Barazany-Gal, Doron Gerber, Amit Tzur
Summary: Protein degradation mediated by the ubiquitin-proteasome pathway plays a crucial role in regulating signaling events. In vitro degradation assays, although laborious, have been important in understanding cell proliferation and other cellular processes. We present a microfluidic technology called protein degradation on chip (pDOC) that enables quick and simultaneous protein degradation assays using minute amounts of reagents. This technology offers a sensitive and high-throughput alternative to conventional degradation assays.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Jiansong Liu, Yichen Zang, Cunying Ma, Dandan Wang, Zhuangfei Tian, Xia Xu, Wenjuan Li, Jihui Jia, Zhifang Liu
Summary: This study reveals that STYX acts as an oncogene in gastric cancer (GC) by inhibiting the degradation function of FBXO31, and may serve as a potential therapeutic target and prognostic biomarker in GC. STYX's interaction with FBXO31 and its regulation by c-Jun and Helicobacter pylori are key mechanisms driving GC progression.
CELL DEATH & DISEASE
(2022)
Review
Agronomy
Keheng Xu, Nan Wu, Wenbo Yao, Xiaowei Li, Yonggang Zhou, Haiyan Li
Summary: The ubiquitin-proteasome pathway (UPP) plays a crucial role in regulating the physiological processes of organisms by specifically removing abnormal peptides and degrading cell regulators. The E3 ubiquitin ligases, especially the Skp1-Cul1-F-box (SCF) complex, are central to UPP and recruit substrate proteins for ubiquitination. F-box proteins, as key components of the SCF complex, are involved in regulating various physiological processes in plants.
Article
Biology
Taylor P. Enrico, Wayne Stallaert, Elizaveta T. Wick, Peter Ngoi, Xianxi Wang, Seth M. Rubin, Nicholas G. Brown, Jeremy E. Purvis, Michael J. Emanuele
Summary: Cell cycle gene expression programs are dysregulated in cancer, and the RB-family of proteins play a key role in repressing cell cycle gene expression and inhibiting proliferation. Phosphorylation and ubiquitination are crucial in cell cycle control, and the SCFcyclin F pathway may regulate the RB-network by affecting p130 degradation, potentially leading to cell cycle dysregulation in human cancers.
Article
Multidisciplinary Sciences
Debasish Paul, Stephen C. Kales, James A. Cornwell, Marwa M. Afifi, Ganesha Rai, Alexey Zakharov, Anton Simeonov, Steven D. Cappell
Summary: The authors develop a fluorescent biosensor to monitor beta-TrCP activity in live cells and find that beta-TrCP remains constitutively active throughout the cell cycle. They also identify receptor-tyrosine kinase signaling as a key regulator of beta-TrCP activity.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Veronika Kinterova, Jiri Kanka, Alexandra Bartkova, Tereza Toralova
Summary: SCF-dependent proteolysis, mediated by SCF (Skp1-Cullin 1-F-box) ligases, plays a crucial role in cell cycle regulation, DNA repair, and centrosome cycle. It is also important in oogenesis and embryogenesis, with SCF beta TrCP and SCFSEL-10/FBXW7 being the most studied ligases in these processes. However, there are still many SCF ligases involved in embryogenesis that need to be further elucidated.
Review
Biochemistry & Molecular Biology
Binshad Badarudeen, Ushma Anand, Swarnendu Mukhopadhyay, Tapas K. Manna
Summary: The centrosome is essential for maintaining genetic stability, ciliogenesis, and cell polarization, consisting of two centrioles that duplicate precisely once during each cell cycle. Recent advances have shown that dedicated ubiquitin ligase-dependent protein degradation machineries regulate centriole duplication by targeting different centriole proteins with intricate mechanisms. This review highlights the coordination of different ubiquitin ligase machineries in controlling centriole duplication.
Article
Biochemistry & Molecular Biology
Amrita Bhattacharya, Vaibhav Kumar Shukla, Nitin Kachariya, Preeti, Parveen Sehrawat, Ashutosh Kumar
Summary: This research report presents the solution structure of the full-length Skp1 protein for the first time and investigates its sequence-dependent dynamics. The results show that Skp1 has a conserved domain structure and substantial variation in motional timescales along its length. These findings provide insight into how Skp1 adapts, binds, and interacts with other proteins in the SCF complex by utilizing its flexibility and conformational sub-states.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jerry Vriend, Thatchawan Thanasupawat, Namita Sinha, Thomas Klonisch
Summary: The ubiquitin proteasome system plays a critical role in maintaining cellular homeostasis and impacting various functions in normal and cancer cells. This study reviewed the role of the UPS in ependymoma brain tumors and identified potential therapeutic targets specific to different subtypes of EPN. The study provides valuable insights into the cellular networks involved in EPN and suggests the UPS as a potential avenue for targeted therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nobuhiro Tanno, Kazumasa Takemoto, Yuki Takada-Horisawa, Ryuki Shimada, Sayoko Fujimura, Naoki Tani, Naoki Takeda, Kimi Araki, Kei-ichiro Ishiguro
Summary: During male meiotic prophase I, FBXO47 plays a crucial role as the stabilizer of the synaptonemal complex, impacting homologous chromosome synapsis, meiotic recombination, and XY body formation.
Article
Biochemistry & Molecular Biology
Christine A. Mills, Xianxi Wang, Dhaval P. Bhatt, Paul A. Grimsrud, Jacob Peter Matson, Debojyoti Lahiri, Daniel J. Burke, Jeanette Gowen Cook, Matthew D. Hirschey, Michael J. Emanuele
Summary: This study identified SIRT5 as a novel substrate of Cyclin F, which regulates cell cycle and metabolism through interaction with Cyclin F. The knockout of SIRT5 results in changes in cell cycle signaling congruent with S and G2/M phase increase. These findings suggest a connection between SIRT5, cell cycle regulation, and metabolism.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Cell Biology
Thomas Tischer, Jing Yang, David Barford
Summary: The control of protein abundance is critical during mitosis, and this study reveals that the APC/C not only regulates mitotic progression, but also plays a role in spindle assembly through its interaction with Cep152.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Martin Reynders, Bryan S. Matsuura, Marleen Berouti, Daniele Simoneschi, Antonio Marzio, Michele Pagano, Dirk Trauner
Editorial Material
Biochemistry & Molecular Biology
Antonio Marzio, Michele Pagano
CELL DEATH AND DIFFERENTIATION
(2020)
Article
Cell Biology
Andras Horvath, Gergely Rona, Michele Pagano, Philip W. Jordan
BMC MOLECULAR AND CELL BIOLOGY
(2020)
Article
Cell Biology
Takaaki Tsunematsu, Rieko Arakaki, Hidehiko Kawai, Jan Ruppert, Koichi Tsuneyama, Naozumi Ishimaru, William C. Earnshaw, Michele Pagano, Yasusei Kudo
JOURNAL OF CELL SCIENCE
(2020)
Article
Cell Biology
Loredana Moro, Daniele Simoneschi, Emma Kurz, Arnaldo A. Arbini, Shaowen Jang, Nicoletta Guaragnella, Sergio Giannattasio, Wei Wang, Yu-An Chen, Geoffrey Pires, Andrew Dang, Elizabeth Hernandez, Payal Kapur, Ankita Mishra, Aristotelis Tsirigos, George Miller, Jer-Tsong Hsieh, Michele Pagano
NATURE CELL BIOLOGY
(2020)
Article
Biology
Hajnalka L. Palinkas, Angela Bekesi, Gergely Rona, Lorinc Pongor, Gabor Papp, Gergely Tihanyi, Eszter Holub, Adam Poti, Carolina Gemma, Simak Ali, Michael J. Morten, Eli Rothenberg, Michele Pagano, David Szuts, Balazs Gyorffy, Beata G. Vertessy
Editorial Material
Cell Biology
Luca Lignitto, Michele Pagano
Summary: Cell-cell fusion, crucial for the development of multicellular organisms, is driven by the remodeling of the actin cytoskeleton. A recent study reveals how CRL3-dependent mono-ubiquitylation regulates cell fusion by controlling the dynamics of cytoskeletal rearrangements.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Marco Salamina, Bailey C. Montefiore, Mengxi Liu, Daniel J. Wood, Richard Heath, James R. Ault, Lan-Zhen Wang, Svitlana Korolchuk, Arnaud Basle, Martyna W. Pastok, Judith Reeks, Natalie J. Tatum, Frank Sobott, Stefan T. Arold, Michele Pagano, Martin E. M. Noble, Jane A. Endicott
Summary: The SCFSKP2 ubiquitin ligase promotes degradation of p27KIP1 to relieve G1 checkpoint control of CDK-cyclin complexes, ensuring orderly cell cycle progression. The interaction between SKP2 and cyclin A provides a structural mechanism for fine-tuning the degradation of p27KIP1 and distinguishes cyclin A from other G1 cyclins.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
M. Heider, Ruth Eichner, Jacob Stroh, Volker Morath, Anna Kuisl, Jana Zecha, Jannis Lawatscheck, Kheewoong Baek, Anne-Kathrin Garz, Martina Rudelius, Friedrich-Christian Deuschle, Ulrich Keller, Simone Lemeer, Mareike Verbeek, Katharina S. Gotze, Arne Skerra, Wolfgang A. Weber, Johannes Buchner, Brenda A. Schulman, Bernhard Kuster, Vanesa Fernandez-Saiz, Florian Bassermann
Summary: This study identifies the role of the CRBN-AHA1-HSP90 axis in the biogenesis of transmembrane proteins, linking IMiD activity to tumor metabolism, and nominating CD98hc and LAT1 as attractive diagnostic and therapeutic targets in MM.
Article
Multidisciplinary Sciences
Emiliano Maiani, Giacomo Milletti, Francesca Nazio, Sos Gronbaek Holdgaard, Jirina Bartkova, Salvatore Rizza, Valentina Cianfanelli, Mar Lorente, Daniele Simoneschi, Miriam Di Marco, Pasquale D'Acunzo, Luca Di Leo, Rikke Rasmussen, Costanza Montagna, Marilena Raciti, Cristiano De Stefanis, Estibaliz Gabicagogeascoa, Gergely Rona, Nelida Salvador, Emanuela Pupo, Joanna Maria Merchut-Maya, Colin J. Daniel, Marianna Carinci, Valeriana Cesarini, Alfie O'sullivan, Yeon-Tae Jeong, Matteo Bordi, Francesco Russo, Silvia Campello, Angela Gallo, Giuseppe Filomeni, Letizia Lanzetti, Rosalie C. Sears, Petra Hamerlik, Armando Bartolazzi, Robert E. Hynds, David R. Pearce, Charles Swanton, Michele Pagano, Guillermo Velasco, Elena Papaleo, Daniela De Zio, Apolinar Maya-Mendoza, Franco Locatelli, Jiri Bartek, Francesco Cecconi
Summary: AMBRA1 has been identified as a key regulator in cell cycle control, playing a crucial role in maintaining genomic integrity by regulating the abundance of D-type cyclins and facilitating the transition from G1 to S phase, thereby preventing replication stress. The findings suggest that CHK1 kinase could be a potential therapeutic target in AMBRA1-deficient tumors, highlighting the importance of the AMBRA1-cyclin D pathway in regulating cell cycle and genomic stability in embryonic development and tumorigenesis.
Article
Multidisciplinary Sciences
Daniele Simoneschi, Gergely Rona, Nan Zhou, Yeon-Tae Jeong, Shaowen Jiang, Giacomo Milletti, Arnaldo A. Arbini, Alfie O'Sullivan, Andrew A. Wang, Sorasicha Nithikasem, Sarah Keegan, Yik Siu, Valentina Cianfanelli, Emiliano Maiani, Francesca Nazio, Francesco Cecconi, Francesco Boccalatte, David Fenyo, Drew R. Jones, Luca Busino, Michele Pagano
Summary: The research identifies AMBRA1 as a ubiquitin ligase that degrades D-type cyclins, whose loss leads to accumulation of D-type cyclins and tumorigenesis. Furthermore, AMBRA1 also affects the sensitivity to CDK4/6 inhibitors.
Article
Multidisciplinary Sciences
Elijah L. Mena, Callie J. Donahue, Laura Pontano Vaites, Jie Li, Gergely Rona, Colin O'Leary, Luca Lignitto, Bearach Miwatani-Minter, Joao A. Paulo, Avantika Dhabaria, Beatrix Ueberheide, Steven P. Gygi, Michele Pagano, J. Wade Harper, Robert A. Davey, Stephen J. Elledge
Summary: The study found an interaction between ORF10 and CRL2ZYG11B but no evidence that ORF10 is functioning to inhibit or hijack CRL2ZYG11B.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Hematology
Jacob Stroh, Anja Seckinger, Michael Heider, Martina Rudelius, Ruth Eichner, Markus Schick, Jolanta Slawska, Martina Emde-Rajaratnam, Hans Salwender, Uta Bertsch, Hartmut Goldschmidt, Katja Weisel, Christof Scheid, Ulrich Keller, Dirk Hose, Florian Bassermann
Summary: In this study, researchers found that MCT1 expression levels were correlated with the survival of patients with multiple myeloma (MM) receiving lenalidomide maintenance therapy. High expression levels of MCT1 were associated with shorter progression-free survival (PFS) and overall survival (OS) in cases with lenalidomide maintenance. However, MCT1 expression did not significantly impact PFS or OS in cases with bortezomib maintenance. These findings suggest that MCT1 can serve as a predictive marker for response to lenalidomide-based maintenance therapy in MM patients.
Article
Oncology
Jiehui Deng, Aatish Thennavan, Igor Dolgalev, Ting Chen, Jie Li, Antonio Marzio, John T. Poirier, David H. Peng, Mirna Bulatovic, Subhadip Mukhopadhyay, Heather Silver, Eleni Papadopoulos, Val Pyon, Cassandra Thakurdin, Han Han, Fei Li, Shuai Li, Hailin Ding, Hai Hu, Yuanwang Pan, Vajira Weerasekara, Baishan Jiang, Eric S. Wang, Ian Ahearn, Mark Philips, Thales Papagiannakopoulos, Aristotelis Tsirigos, Eli Rothenberg, Justin Gainor, Gordon J. Freeman, Charles M. Rudin, Nathanael S. Gray, Peter S. Hammerman, Michele Pagano, John V. Heymach, Charles M. Perou, Nabeel Bardeesy, Kwok-Kin Wong
Summary: The study found that LKB1-deficient lung tumors are sensitive to autophagy inhibition, which can restore impaired antigen presentation and antitumor immune responses, enhancing the effectiveness of PD-1 immunotherapy. Additionally, LKB1 deficiency inhibits antigen processing and presentation, but this can be reversed by targeting the autophagy pathway to restore immunoproteasome activity and antigen presentation.
Article
Oncology
Loredana Moro, Michele Pagano
MOLECULAR & CELLULAR ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)