Review
Endocrinology & Metabolism
Imke L. Lemmer, Nienke Willemsen, Nazia Hilal, Alexander Bartelt
Summary: This review provides a general guide to understanding the nature, etiology, and consequences of ER stress in metabolic disorders, emphasizing key mechanisms such as protein quality control, lipid and carbohydrate metabolism, and inflammation.
MOLECULAR METABOLISM
(2021)
Article
Environmental Sciences
Xiaoyu Wang, Fan Yang, Xiaomin Tian, Haihua Huo, Xinrun Li, Haitong Wu, Jianying Guo
Summary: Excessive copper can cause histopathological damage to duck cerebrums, disrupt the balance of trace elements, induce oxidative stress and activation of ER quality control, resulting in duck cerebrums damage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Cardiac & Cardiovascular Systems
Leilei Hu, Dongjie Gao, Hao Lv, Lu Lian, Mingyang Wang, Yunjiao Wang, Yingyu Xie, Junping Zhang
Summary: Heart failure is a progressive disease with a high mortality rate and a significant socioeconomic burden. Endoplasmic reticulum stress and autophagy have been identified as important factors in the development of heart failure and potential targets for pharmacological intervention. However, the mechanism linking the two is not fully understood. This review aims to explore the effects and interactions of endoplasmic reticulum stress and autophagy in heart failure, providing insights for future targeted therapies.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2023)
Review
Medicine, Research & Experimental
Shengkai Xia, Wenzhe Duan, Wenwen Liu, Xinri Zhang, Qi Wang
Summary: GRP78 is a key protein in the pathological processes of lung cancer, involved in protein folding, cellular stress response to microenvironment stimuli, and closely associated with the progression and poor prognosis of lung cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Yi Sheng, Guang Yang, Zachary Markovich, Sung Min Han, Rui Xiao
Summary: Proteotoxic stress is a common challenge for all organisms, and the evolutionarily conserved unfolded protein responses (UPRs) play a key role in defending such stress. Different subcellular compartments show distinct temporal regulation of UPRs during aging, with UPRs elevating with age but their inducibility declining. Different types of UPRs have varied temporal requirements for induction, and different tissues may exhibit distinct temporal profiles of UPR inducibility during aging.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Cell Biology
Ming Yang, Shilu Luo, Xi Wang, Chenrui Li, Jinfei Yang, Xuejing Zhu, Li Xiao, Lin Sun
Summary: The endoplasmic reticulum (ER) is crucial for maintaining cellular homeostasis, and ER-phagy, a selective form of autophagy, helps remove damaged ER to protect cells from harm caused by excessive ER stress. Many receptor-mediated ER-phagy pathways have been discovered in recent years, highlighting the importance of this newly identified autophagy process in maintaining cellular health.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Debora Gentile, Marianna Esposito, Paolo Grumati
Summary: Autophagy is a cellular cleaning system that maintains metabolic balance and plays a crucial role in sustaining malignant cellular growth during tumorigenesis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chang Ho Kang, Eun Seon Lee, Ganesh M. Nawkar, Joung Hun Park, Seong Dong Wi, Su Bin Bae, Ho Byoung Chae, Seol Ki Paeng, Jong Chan Hong, Sang Yeol Lee
Summary: The key regulator of light signaling, COP1, plays a crucial role in plant endoplasmic reticulum stress response by degrading HY5 in the nucleus, facilitating the unfolded protein response activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Aleksandra Ochneva, Yana Zorkina, Olga Abramova, Olga Pavlova, Valeriya Ushakova, Anna Morozova, Eugene Zubkov, Konstantin Pavlov, Olga Gurina, Vladimir Chekhonin
Summary: This review discusses the potential contribution of protein illnesses in the development of psychopathologies. It describes the possible mechanisms of disruption to protein folding and aggregation in the cell, as well as the known proteins whose aggregation has been observed in psychiatric disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Adalberto Merighi, Laura Lossi
Summary: In addition to protein processing, the endoplasmic reticulum (ER) also plays roles in lipid synthesis, molecular transfers, and Ca2+ homeostasis. Proper protein folding and modification in the ER require molecular chaperones and a favorable environment. Disruption in ER conditions or chaperone capacity can lead to the unfolded protein response (UPR), triggering autophagy and potentially causing cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Akitoshi Nakashima, Tomoko Shima, Sayaka Tsuda, Aiko Aoki, Mihoko Kawaguchi, Atsushi Furuta, Ippei Yasuda, Satoshi Yoneda, Akemi Yamaki-Ushijima, Shi-Bin Cheng, Surendra Sharma, Shigeru Saito
Summary: Aggrephagy is the selective degradation of aggregated proteins by autophagosomes, and cells have mechanisms to combat the accumulation of aggregates through refolding, translational inhibition, and degradation. Protein aggregates found in preeclamptic placentas include those associated with neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gehan Botrus, Richard M. Miller, Pedro Luiz Serrano Uson Jr, Geoffrey Kannan, Haiyong Han, Daniel D. Von Hoff
Summary: High levels of ER stress and UPR activation are present in pancreatic cancer, leading to adaptive mechanisms and potential apoptosis. This review discusses the mechanisms by which compounds activate the UPR pathways and induce apoptosis, and explores the potential of ER stress inducers for anti-tumor efficacy in pancreatic cancer. A new approach of increasing ER stress and UPR activation to incite apoptotic cell death in pancreatic cancer is hypothesized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Junhee Kwon, Jihyun Kim, Keun Il Kim
Summary: Cells activate protective mechanisms to overcome stressful conditions and protect themselves. ER stress induces an unfolded protein response (UPR) and usually activates autophagy for cytoprotection. However, sustained activation of ER stress and autophagy can lead to cell death and treatment resistance. Understanding the relationship between ER stress and autophagy is important for developing therapies for various diseases.
ANIMAL CELLS AND SYSTEMS
(2023)
Review
Plant Sciences
Sofia Reyes-Impellizzeri, Adrian A. Moreno
Summary: This review focuses on the impact of alterations in components of ERQC, ERAD, and UPR on plant responses to abiotic stresses. Evidence shows a clear connection between ERAD and UPR mechanisms, but the connection of ERQC components with these processes or their possible client proteins is lacking. Proteomics approaches are suggested to uncover the identity of these proteins and their connection to ER proteostasis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biology
Vladislav Belyy, Iratxe Zuazo-Gaztelu, Andrew Alamban, Avi Ashkenazi, Peter Walter
Summary: Protein folding homeostasis in the endoplasmic reticulum is regulated by the unfolded protein response (UPR), with Inositol-requiring enzyme 1 (IRE1) playing a key role in signal transmission. This study used a two-color single-molecule tracking approach to investigate the oligomerization of IRE1 in live cells. The results suggest that IRE1 exists as a constitutive homodimer and assembles into small oligomers upon ER stress, with its lumenal domain governing the formation of inactive dimers and stress-dependent oligomers.
Review
Biochemistry & Molecular Biology
Danilo B. Medinas, Pablo Rozas, Claudio Hetz
Summary: Protein disulfide isomerases (PDIs) play a crucial role in oxidoreductase folding and quality control in the endoplasmic reticulum, and have significant implications for cellular homeostasis and neurological diseases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Magda C. Diaz-Vesga, Raul Flores-Vergara, Jaime A. Riquelme, Marcelo Llancaqueo, Gina Sanchez, Cecilia Vergara, Luis Michea, Paulina Donoso, Andrew F. G. Quest, Ivonne Olmedo, Zully Pedrozo
Summary: Cardiomyopathy is commonly observed in patients with ADPKD, even without renal dysfunction or high blood pressure. This study investigated the role of PC1 in cardiovascular pathophysiology and found that decreased PC1 expression in cardiomyocytes induces dilated cardiomyopathy through reduced BIN1 expression and T-tubule remodeling. These findings suggest a novel pathway that may contribute to the development of cardiomyopathy in ADPKD patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Correction
Medicine, Research & Experimental
Stephanie Lhomond, Tony Avril, Nicolas Dejeans, Konstantinos Voutetakis, Dimitrios Doultsinos, Mari McMahon, Raphael Pineau, Joanna Obacz, Olga Papadodima, Florence Jouan, Heloise Bourien, Marianthi Logotheti, Gwenaele Jegou, Nestor Pallares-Lupon, Kathleen Schmit, Pierre-Jean Le Reste, Amandine Etcheverry, Jean Mosser, Kim Barroso, Elodie Vauleon, Marion Maurel, Afshin Samali, John B. Patterson, Olivier Pluquet, Claudio Hetz, Veronique Quillien, Aristotelis Chatziioannou, Eric Chevet
EMBO MOLECULAR MEDICINE
(2023)
Review
Pharmacology & Pharmacy
Mayarling F. Troncoso, Magda C. Diaz-Vesga, Fernanda Sanhueza-Olivares, Jaime A. Riquelme, Marioly Muller, Luis Garrido, Luigi Gabrielli, Mario Chiong, Ramon Corbalan, Pablo F. Castro, Sergio Lavandero
Summary: This review discusses the potential targeting of VCAM-1 in atherosclerosis, diabetes, hypertension, and ischemia/reperfusion injury, and highlights that VCAM-1 may be a promising therapeutic target for vascular diseases.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2023)
Article
Biochemistry & Molecular Biology
Lautaro Briones-Suarez, Mariana Cifuentes, Roberto Bravo-Sagua
Summary: Obesity-induced inflammation in adipose tissue has harmful effects on organs like the liver. The activation of the calcium-sensing receptor in pre-adipocytes results in the secretion of pro-inflammatory cytokines, TNF-alpha, and IL-1 beta. These cytokines promote cell senescence and mitochondrial dysfunction in HepG2 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Pia Apablaza, Juan Carlos Borquez, Rodrigo Mendoza, Monica Silva, Gladys Tapia, Alejandra Espinosa, Rodrigo Troncoso, Luis A. Videla, Nevenka Juretic, Andrea del Campo
Summary: Increase in body fat leads to changes in skeletal muscle and accelerates sarcopenia, known as sarco-obesity or sarcopenic obesity. Obesity affects the skeletal muscle's ability to oxidize glucose and causes mitochondrial dysfunction. Exercise improves mitochondrial dysfunction, but the effects on the mitochondrial unfolded protein response (UPRmt) in the skeletal muscle are not well understood. This study aimed to investigate the UPRmt response to exercise in obese mice and its association with skeletal muscle function improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Kizito-Tshitoko Tshilenge, Carlos Galicia Aguirre, Joanna Bons, Akos A. Gerencser, Nathan Basisty, Sicheng Song, Jacob Rose, Alejandro Lopez-Ramirez, Swati Naphade, Ashley Loureiro, Elena Battistoni, Mateus Milani, Cameron Wehrfritz, Anja Holtz, Claudio Hetz, Sean D. Mooney, Birgit Schilling, Lisa M. Ellerby
Summary: Huntington's disease is a neurodegenerative disorder caused by a genetic mutation, leading to changes in protein function. By analyzing the proteome of human patient-derived neurons, researchers identified dysregulated pathways relevant to the disease, including extracellular matrix and DNA signaling for upregulated proteins, and neurogenesis and neurotrophic factor signaling for downregulated proteins.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Claudia Duran-Aniotz, Natalia Poblete, Catalina Rivera-Krstulovic, alvaro O. Ardiles, Mei Li Diaz-Hung, Giovanni Tamburini, Carleen Mae P. Sabusap, Yannis Gerakis, Felipe Cabral-Miranda, Javier Diaz, Matias Fuentealba, Diego Arriagada, Ernesto Munoz, Sandra Espinoza, Gabriela Martinez, Gabriel Quiroz, Pablo Sardi, Danilo B. Medinas, Darwin Contreras, Ricardo Pina, Mychael V. Lourenco, Felipe C. Ribeiro, Sergio T. Ferreira, Carlos Rozas, Bernardo Morales, Lars Plate, Christian Gonzalez-Billault, Adrian G. Palacios, Claudio Hetz
Summary: Alteration in proteostasis network buffering capacity is a new feature of Alzheimer's disease, indicating the presence of endoplasmic reticulum stress. The unfolded protein response (UPR) is the main pathway to deal with ER stress caused by protein folding. Inositol-requiring enzyme-1 (IRE1) serves as a central ER stress sensor, controlling the expression of the transcription factor X-box binding protein 1 (XBP1) to establish adaptive and repair programs. Artificially enhancing the UPR capacity in the AD brain by expressing the active form of XBP1 reduces amyloid deposits and improves synaptic and cognitive function.
Review
Physiology
Roberto Bravo-Sagua, Camila Lopez-Crisosto, Alfredo Criollo, Reiko Inagi, Sergio Lavandero
Summary: Organelles are membrane-lined structures that compartmentalize subcellular biochemical functions. Interorganelle communication is crucial for cellular responses. Evidence suggests that other organelles play important roles in pathophysiological conversation.
Article
Pharmacology & Pharmacy
Nicolas Herrera-Zelada, Ursula Zuniga-Cuevas, Andres Ramirez-Reyes, Ignacio Norambuena-Soto, Leslye Venegas-Zamora, Mayarling F. Troncoso, Alejandra Hernandez, Gina Sanchez, Zully Pedrozo, Sergio Lavandero, Jaime A. Riquelme
Summary: Small extracellular vesicles (SEVs), nanosized vesicles that transport biomolecules between cells, have potential applications as biomarkers, drug delivery tools, and therapeutic agents. SEVs of endothelial origin have been shown to reduce in vitro ischemia/reperfusion (I/R) injury, but the impact of endothelial inflammation on SEV functionality is unclear.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Hugo E. E. Verdejo, Adolfo Rojas, Camila Lopez-Crisosto, Fernando Baraona, Luigi Gabrielli, Vinicius Maracaja-Coutinho, Mario Chiong, Sergio Lavandero, Pablo F. F. Castro
Summary: This study investigated the role of trimetazidine in patients with PAH and found that it improved right ventricular function, remodeling, and functional class. Trimetazidine use was safe and well-tolerated, and it led to increased exercise capacity and minor improvements in RV remodeling.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Nutrition & Dietetics
Matias Monsalves-Alvarez, Teresa Jimenez, Daniel Bunout, Gladys Barrera, Sandra Hirsch, Carlos Sepulveda-Guzman, Claudio Silva, Juan M. Rodriguez, Rodrigo Troncoso, Maria Pia de la Maza
Summary: This study compares the body composition and metabolic changes in overweight and obese Chilean women and men after 3 months of weight loss treatment with a Mediterranean-type hypocaloric diet, high-intensity interval training (HIIT), or both. The results show that the hypocaloric diet mainly reduces fat mass but causes a loss of skeletal muscle mass, which can be prevented by HIIT.
FRONTIERS IN NUTRITION
(2023)
Article
Cell Biology
Mateus Milani, Philippe Pihan, Claudio Hetz
Summary: Calcium is a crucial messenger in regulating cellular processes and interorganelle communication. Lysosomal function is dependent on calcium, and ion channels on the lysosomal membrane regulate lysosomal properties such as pH. Lysosome-dependent cell death (LDCD) is a specific type of cell death involving lysosomes that plays important roles in tissue homeostasis, development, and pathology. This article focuses on recent advances in understanding the role of calcium signaling in LDCD.
Article
Biology
Carlos Sepulveda, Juan Manuel Rodriguez, Matias Monsalves-Alvarez, Camila Donoso-Barraza, Francisco de la Fuente, Isabelle Matias, Thierry Leste-Lasserre, Philippe Zizzari, Eugenia Morselli, Daniela Cota, Miguel Llanos, Rodrigo Troncoso
Summary: The endocannabinoid system (ECS) regulates energy metabolism and is involved in the pathogenesis of metabolic diseases. Autophagy is associated with various cellular processes and its deregulation is linked to diseases. This study found that the CB1 receptor controls autophagy in the skeletal muscle of both lean and obese mice.
BIOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)