期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1833, 期 8, 页码 1876-1884出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2013.03.009
关键词
MPP1/p55; Membrane rafts; Palmitoylatibn; Erythroid/HEL cells; MPP1 silencing; FLIM technique
资金
- Polish Ministry of Science and Higher Education [N N301 1611435]
- EU Social Fund [POKL.04.01.01-00-054/10-00]
Here we show the crucial role of MPP1 in lateral membrane ordering/organization in HEL cells (derived from erythroid precursors). Biochemical analyses showed that inhibition of MPP1 palmitoylation or silencing of the MPP1 gene led to a dramatic decrease in the DRM fraction. This was accompanied by a reduction of membrane order as shown by fluorescence-lifetime imaging microscopy (FLIM) analyses. Furthermore, MPP1 knockdown significantly affects the activation of MAP-kinase signaling via raft-dependent RTK (receptor tyrosine kinase) receptors, indicating the importance of MPP1 for lateral membrane organization. In conclusion, palmitoylation of MPP1 appears to be at least one of the mechanisms controlling lateral organization of the erythroid cell membrane. Thus, this study, together with our recent results on erythrocytes, reported elsewhere (Lach et al., J. Biol. Chem., 2012, 287, 18974-18984), points to a new role for MPP1 and presents a novel linkage between membrane raft organization and protein palmitoylation. (C) 2013 Elsevier B.V. All rights reserved.
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