Article
Plant Sciences
Larissa Machado Antonio, Gustavo Henrique Martins, Annelize Zambon Barbosa Aragao, Natalia Galdi Quel, Gabriel Zazeri, Walid A. Houry, Carlos Henrique Inacio Ramos
Summary: The chaperone R2TP plays an important role in protein assembly and its study in plant systems provides valuable insights. The protein RPAP3 in Sorghum bicolor has been identified and shown to have similar functions as its counterparts in yeast and humans. Understanding the role of RPAP3 in plant R2TP complex can contribute to advancements in plant biology and biotechnological applications.
Review
Biochemistry & Molecular Biology
Jeffrey Lynham, Walid A. Houry
Summary: Hsp90 is a ubiquitous molecular chaperone that plays an important role in cell signaling pathways. Its interactions with specific chaperones and cochaperones determine the folding of client proteins. Although it has multiple roles in protein complex assembly, the detailed mechanisms are still not fully understood.
Article
Biochemistry & Molecular Biology
Thiago Seraphim, Nardin Nano, Yiu Wing Sunny Cheung, Siripat Aluksanasuwan, Carolina Colleti, Yu-Qian Mao, Vaibhav Bhandari, Gavin Young, Larissa Holl, Sadhna Phanse, Yuliya Gordiyenko, Daniel R. Southworth, Carol Robinson, Visith Thongboonkerd, Lisandra M. Gava, Julio C. Borges, Mohan Babu, Leandro R. S. Barbosa, Carlos H. Ramos, Philipp Kukura, Walid A. Houry
Summary: The R2TP complex, formed by RUVBL1 and RUVBL2 ATPases, associates with PIH1D1 and RPAP3 proteins and plays a role in promoting the formation of macromolecular complexes. RPAP3 is identified as the central subunit of R2TP, linking PIH1D1 and RUVBL1/2. The study provides insights into the structure and function of the R2TP complex.
Article
Multidisciplinary Sciences
Chloe Maurizy, Claire Abeza, Benedicte Lemmers, Monica Gabola, Ciro Longobardi, Valerie Pinet, Marina Ferrand, Conception Paul, Julie Bremond, Francina Langa, Francois Gerbe, Philippe Jay, Celine Verheggen, Nicola Tinari, Dominique Helmlinger, Rossano Lattanzio, Edouard Bertrand, Michael Hahne, Berengere Pradet-Balade
Summary: The R2TP chaperone plays essential roles in intestinal cell proliferation and colorectal cancer, with Rpap3 deletion inhibiting cell proliferation in mice intestine, while elevated RPAP3 levels in colorectal tumors are associated with poor prognosis.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Anuradhika Puri, Priyanka Singh, Navinder Kumar, Rajesh Kumar, Deepak Sharma
Summary: Prions are self-perpetuating infectious proteins. Tah1 is identified as a novel modulator of the yeast prion [URE3], influencing its formation and maintenance. The Hsp90 co-chaperone Tah1 is crucial for the assembly of small nucleolar ribonucleoprotein complexes.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biology
Yang S. Chen, Wanfu Hou, Sharon Tracy, Alex T. Harvey, Vince Harjono, Fan Xu, James J. Moresco, John R. Yates, Brian M. Zid, Alan G. Hinnebusch
Summary: In this study, it was found that Rvb1/Rvb2 proteins can regulate gene expression during glucose starvation by binding to mRNAs and repressing their translation. Additionally, depletion of Rvb2 leads to decreased mRNA induction of alternative glucose metabolism genes but increased protein synthesis during glucose starvation.
Article
Cell Biology
Amanda J. Smith, Ximena M. Bustamante-Marin, Weining Yin, Patrick R. Sears, Laura E. Herring, Nedyalka N. Dicheva, Francesc Lopez-Giraldez, Shrikant Mane, Robert Tarran, Margaret W. Leigh, Michael R. Knowles, Maimoona A. Zariwala, Lawrence E. Ostrowski
Summary: Mutations in SPAG1, a dynein axonemal assembly factor, cause primary ciliary dyskinesia. This study found that SPAG1 interacts with multiple proteins and plays a role in assembly of dynein arms. A previously uncharacterized SPAG1 isoform was also identified.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Gabriele Giachin, Matthew Jessop, Romain Bouverot, Samira Acajjaoui, Melissa Saidi, Anais Chretien, Maria Bacia-Verloop, Luca Signor, Philippe J. Mas, Adrien Favier, Eve Borel Meneroud, Michael Hons, Darren J. Hart, Eaazhisai Kandiah, Elisabetta Boeri Erba, Alain Buisson, Gordon Leonard, Irina Gutsche, Montserrat Soler-Lopez
Summary: ECSIT functions as the bridging node of the MCIA core complex, inducing the transformation of ACAD9 from a FAO enzyme to an MCIA factor, providing a unique molecular mechanism for coordinating the regulation of the FAO and OXPHOS pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Yoann Abel, Christophe Charron, Camille Virciglio, Valerie Bourguignon-Igel, Marc Quinternet, Marie-Eve Chagot, Marie-Cecile Robert, Celine Verheggen, Christiane Branlant, Edouard Bertrand, Xavier Manival, Bruno Charpentier, Mathieu Rederstorff
Summary: In this study, it was found that the R2TP component RPAP3 binds to TRBP rather than PACT, and the binding of TRBP to RPAP3 or Dicer is mutually exclusive. Additionally, it was discovered that AGO(1/2), TRBP, and Dicer are all sensitive to HSP90 inhibition, with increased sensitivity of TRBP in the absence of RPAP3. Furthermore, RPAP3 appears to impede miRNA activity, suggesting that the R2TP chaperone might sequester TRBP to regulate the miRNA pathway.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Moien Lone, Mahaiwon Shadang, Qulsum Akhter, Mithilesh Kumar, Saumyaranjan Mallick, Ajay Gogia, Nilima Nilima, Shyam S. Chauhan, Riyaz A. Mir
Summary: This study found that immunopositivity for RUVBL1 in DLBCL patients is associated with poor prognosis and a higher relapse rate. Additionally, immunopositivity for PIH1D1 is correlated with a higher international prognostic index score.
Article
Chemistry, Multidisciplinary
Lanqin Tang, Thi Vo, Xiaoxing Fan, Drew Vecchio, Tao Ma, Jun Lu, Harrison Hou, Sharon C. Glotzer, Nicholas A. Kotov
Summary: The research discovered that uniformly sized CdS-based hedgehog particles are self-assembled from polydisperse nanoparticles with diameters ranging from 1.0 to 4.0 nm, forming various geometric shapes and topological structures under different conditions. These particles exhibit unusually high dispersion stability in unfavorable solvents like liquid CO2, and understanding the mechanism of their formation is crucial for adapting them for various applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Xizi Chen, Yilun Qi, Zihan Wu, Xinxin Wang, Jiabei Li, Dan Zhao, Haifeng Hou, Yan Li, Zishuo Yu, Weida Liu, Mo Wang, Yulei Ren, Ze Li, Huirong Yang, Yanhui Xu
Summary: This study elucidates the assembly mechanism of human TFIID-based PIC, showing two tracks for modular reorganization and direct promoter deposition, converging at a holo PIC with around 50 subunits. TFIID stabilizes PIC organization and supports CAK-mediated phosphorylation of Pol II.
Review
Chemistry, Inorganic & Nuclear
Robin R. Groleau, Tony D. James, Steven D. Bull
Summary: This review discusses a method for measuring the enantiopurities of chiral molecules, focusing on the Bull-James assembly and other analytical techniques which can be applied to high-throughput ee analysis. Examples of applications in constructing various compounds and preparing labeled biomolecules are also presented.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Biochemical Research Methods
Tianjiao Zhang, Jie Zhou, Wentao Gao, Yuran Jia, Yanan Wei, Guohua Wang
Summary: High-quality genome chromosome-scale sequences are crucial for genomics downstream analysis, especially in the construction of haplotype-resolved and complete genomes. The emergence of long-read sequencing technology has greatly improved complex genome assembly. Among various computational methods, uncollapsed assembly is the most accurate and complete way to represent genomes. Genome assembly is closely related to haplotype reconstruction.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Chemistry, Multidisciplinary
Ru-Yu Hua, Si-Fan Yu, Xiao-Ting Jie, Huang Qiu, Wen-Hao Hu
Summary: Chiral oxindoles are important chemical structures found in many natural products. While there are well-developed methods for constructing C3 quaternary oxindoles with high selectivity, the efficient synthesis of chiral 3-substituted tertiary oxindoles is rarely reported. In this study, a multicomponent assembly method for complex oxindoles through enantioselective cooperative catalysis is presented.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)