Article
Plant Sciences
Valentin Hammoudi, Bas Beerens, Martijs J. Jonker, Tieme A. Helderman, Georgios Vlachakis, Marcel Giesbers, Mark Kwaaitaal, Harrold A. van den Burg
Summary: SUMO plays a critical role in plant thermoresilience and longevity, particularly at 28 degrees Celsius. Mutants with reduced levels of SUMO1/2 exhibit thermolethality at 28 degrees Celsius, but not due to autoimmunity. The role of SUMO in thermoresilience is distinct from its requirement in thermomorphogenesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Cell Biology
Shuo Zhang, Peng Wang, Binwu Hu, Weijian Liu, Xiao Lv, Songfeng Chen, Zengwu Shao
Summary: This study found that the HSP90 inhibitor 17-AAG can attenuate inflammation and catabolism induced by M1 macrophages in the nucleus pulposus. 17-AAG exerts anti-inflammatory and anti-catabolic effects by modulating the activities of macrophages and NPCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agronomy
Hsien-Tzu Wang, Yu-Jung Cheng, Jhih-Ting Hsiao, Fuu Sheu, Yen-Chou Kuan
Summary: This study investigated the expression of broccoli HSP and the effect of 17-AAG on broccoli senescence. Through RNA-sequencing, 11 HSP and related genes were identified, with 10 nM 17-AAG treatment significantly alleviating yellowing of broccoli and enhancing stress tolerance. This research highlights the potential of using HSP inducers like 17-AAG to improve shelf life and quality of postharvest crops.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jen-Chung Ko, Jyh-Cheng Chen, Jou-Min Hsieh, Pei-Yu Tseng, Chen -Shan Chiang, Li -Ling Liu, Chin-Cheng Chien, I-Hsiang Huang, Qiao-Zhen Chang, Bo-Cheng Mu, Yun-Wei Lin
Summary: This study found that the Hsp90 inhibitor 17-AAG can enhance the cytotoxicity of tamoxifen and erlotinib in NSCLC cells by regulating TP expression.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Chemistry, Medicinal
Jingnan Chen, Yu Wang, Rongtian Wang, Rujing Yuan, Guo-Chao Chu, Yi-Ming Li
Summary: Transiently-activated SUMO probes are useful for understanding the dynamic control of SENPs activity. In this study, we developed a photocaged glycine-assisted strategy to construct on demand-activated SUMO-ABPs. Light-sensitive groups installed at specific sites of SUMO-2 temporarily block probe activity and hinder aspartimide formation, allowing the synthesis of inert SUMO-2 prop-argylamide. The probe can be activated to capture SENPs both in vitro and in intact cells, enabling intracellular time-resolved proteome-wide profiling of SUMO-related enzymes.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Multidisciplinary Sciences
Chaitanya A. Patwardhan, Vamsi Krishna Kommalapati, Taoufik Llbiyi, Digvijay Singh, Eyad Alfa, Anatolij Horuzsko, Hasan Korkaya, Siva Panda, Christopher A. Reilly, Vladimir Popik, Ahmed Chadli
Summary: Capsaicin inhibits Hsp90 and has potential for cancer drug development.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Krishna M. Chauhan, Yingxiao Chen, Yiyi Chen, Andrew T. Liu, Xiao-Xin Sun, Mu-Shui Dai
Summary: The study reveals that SENP1 is a critical p53 deSUMOylating enzyme, and its depletion induces p53 transactivation activity and synergizes with DNA damage-inducing agent etoposide to activate p53 and inhibit cancer cell growth.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Chemistry, Medicinal
Faiyaz Ahmad, Shweta Sharma, Smita Yadav, Sushma Rathaur
Summary: This study observed the effect of 17-N-allyl-17-demethoxygeldanamycin on adult filarial parasites, finding that it induced oxidative stress, altered calcium and proteostasis, leading to apoptosis.
DRUG DEVELOPMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xiaoxia Shi, Yixin Du, Shujing Li, Huijian Wu
Summary: SUMOylation, a reversible post-translational modification process, plays crucial roles in cell physiology, including cell cycle regulation, DNA damage repair, and protein trafficking and turnover. Dysregulated SUMO E3 ligases may be associated with the occurrence and development of cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shuji Ueda, Moeka Nishihara, Yuuki Hioka, Ken-ichi Yoshino, Soichiro Yamada, Minoru Yamanoue, Yasuhito Shirai
Summary: Protein homeostasis, including folding, refolding, and degradation, is believed to decline with aging. HSPB5 acts as a molecular chaperone and prevents protein aggregation. In this study, we discovered a new binding protein for HSPB5 called PLK2, which is involved in apoptosis. PLK2 was upregulated in cells treated with MG132 and co-localized with HSPB5 near the ER. Inhibition of PLK2 increased apoptosis and decreased ER stress-induced phosphorylation of HSPB5. These findings suggest the importance of HSPB5 phosphorylation during ER stress and highlight the essential role of the PLK2/HSPB5 pathway in cytoprotection against proteasome inhibition-induced ER stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medical Laboratory Technology
Wenhui Liu, Min Zeng, Nian Fu
Summary: The nuclear receptor superfamily, a family of ligand-activated transcription factors, plays a key role in cell metabolism and human diseases. They undergo various post-translational modifications, influencing diverse physiological activities. Nuclear receptors are crucial in lipid metabolism, inflammation, while SUMOylation nuclear receptors regulate function and impact cell metabolism.
CLINICA CHIMICA ACTA
(2021)
Article
Microbiology
Junping Li, Libin Liang, Li Jiang, Qian Wang, Xia Wen, Yuhui Zhao, Pengfei Cui, Yaping Zhang, Guangwen Wang, Qibing Li, Guohua Deng, Jianzhong Shi, Guobin Tian, Xianying Zeng, Yongping Jiang, Liling Liu, Hualan Chen, Chengjun Li
Summary: Our study demonstrates the vital role of PB1 K612 SUMOylation in the pathogenesis and transmission of IAVs, providing a potential target for anti-influenza therapies. The SUMOylation at K612 is crucial for PB1 to efficiently bind viral RNA, facilitating the transcription and replication of the viral genome.
Review
Plant Sciences
Shumei Fang, Xue Hou, Xilong Liang
Summary: SUMOylation is a crucial posttranslational modification in eukaryotes, involved in metabolic regulation under various stresses. SIZ1, a SUMO E3 ligase, plays a vital role in substrate-specific recognition and facilitates the binding of SUMO proteins to target proteins. This review provides insights into the characteristics of SUMOylation and the role of SIZ1 in plant adaptation to abiotic stresses, offering valuable references for crop improvement and future research on plant responses to environmental stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Mingchao Mu, Qin Zhang, Jing Li, Chenye Zhao, Xiaopeng Li, Zilu Chen, Xuejun Sun, Junhui Yu
Summary: The current study reveals that USP51 promotes colorectal cancer stemness and chemoresistance, and its high expression is associated with poor survival in colorectal cancer patients. USP51 forms a positive feedback loop with HIF1A, regulating its deubiquitination and stabilization. The interaction between USP51 and ELOC is facilitated by the deSUMOylation of ELOC mediated by SENP1. Importantly, USP51 plays a crucial role in promoting HIF1A and SENP1-dependent proliferation, migration, stemness, and chemoresistance under hypoxia in colorectal cancer.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Plant Sciences
Therese C. Rytz, Juanjuan Feng, Jessica A. S. Barros, Richard D. D. Vierstra
Summary: The reversible conjugation of SUMO to other proteins plays an important role in plant development and stress defense. SUMO can be further modified by SUMOylation and ubiquitylation, but the functions of these modifications are not well understood. By replacing the essential SUMO isoforms with a lysine-null variant, the researchers found that these secondary modifications are involved in stress defense, DNA repair, and hormone signaling. The study also generated a new tool for investigating SUMO in plants.
Article
Food Science & Technology
Silvie Rimpelova, Tomas Zimmermann, Pavel B. Drasar, Bohumil Dolensky, Jiri Bejcek, Eva Kmonickova, Petra Cihlarova, Sona Gurska, Lucie Kuklikova, Marian Hajduch, Tomas Ruml, Lubomir Opletal, Petr Dzubak, Michal Jurasek
Summary: Cardiac glycosides (CGs) are a diverse group of natural compounds, with most being potent inhibitors of Na+/K+-ATPase used in treating cardiac conditions. Some lesser known CGs may have anticancer potential, but currently used CGs have limitations in cancer treatment. This study focused on two poorly described CGs, hyrcanoside and deglucohyrcanoside, with the hope of them serving as research tools and novel cancer therapeutics.
Article
Chemistry, Medicinal
Bara Krizkovska, Rohitesh Kumar, Katerina Rehorova, David Sykora, Simona Dobiasova, Denisa Kucerova, Maria Carmen Tan, Virgilio Linis, Glenn Oyong, Tomas Ruml, Jan Lipov, Jitka Viktorova
Summary: This study compared the composition and biological activities of methanolic and dichloromethane extracts from eight Selaginella species, with flavonoids predominating among the identified 193 compounds. Methanolic extracts showed higher antioxidant capacity and cytotoxicity against cancer cell lines, while some extracts exhibited inhibition of acetylcholinesterase activity. Particularly, Selaginella apoda showed promising potential for further exploration.
Article
Plant Sciences
Rohitesh Kumar, Jitka Viktorova, Bara Krizkovska, Jan Lipov, Tomas Ruml
Summary: This review provides a comprehensive list of unique secondary metabolites isolated from the genus Selaginella and also provides insight into their important biological activities. The review is divided into two main sections: one elaborates on compounds exclusive to this genus, while the other discusses a number of different pharmacological activities exhibited by the compounds and Selaginella extracts.
PHYTOCHEMISTRY REVIEWS
(2021)
Article
Multidisciplinary Sciences
Eva Jablonska, Jiri Kubasek, Dalibor Vojtech, Tomas Ruml, Jan Lipov
Summary: The concentration of serum, type of medium, type of exposition, and type of cells have a significant impact on in vitro cytotoxicity testing. Therefore, careful specification of testing conditions and cautious comparison of different studies should be conducted when evaluating the cytotoxicity of biomaterials.
SCIENTIFIC REPORTS
(2021)
Review
Biology
Jan Skubnik, Vladimira Svobodova Pavlicova, Tomas Ruml, Silvie Rimpelova
Summary: Vincristine is a commonly used compound in cancer treatment, known for its strong anticancer activity but also for its neurotoxic side effects. Despite limitations, it continues to play a crucial role in combination therapy.
Article
Biochemistry & Molecular Biology
Martina Koncosova, Nikola Vrzackova, Ivana Krizova, Petra Tomasova, Silvie Rimpelova, Ales Dvorak, Libor Vitek, Michaela Rumlova, Tomas Ruml, Jaroslav Zelenka
Summary: Metabolic transformation in cancer cells leads to lactate accumulation and acidification in the tumor microenvironment, affecting cancer progression and patient prognosis. Cells adapted to acidosis are sensitive to oxidative damage, with lactate concentrations playing a role in sensitization. The drug CPI-613 can selectively eradicate acidosis-adapted cancer cells and the use of tetracycline can enhance its cytotoxic effect. Lactate metabolism in the Krebs cycle restores antioxidant capacity under acidosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Dagmar Polakova, Lukas Capek, Martin Bartos, Pavel Kejzlar, Miroslava Rysova, Lenka Martinova, Alena Sevcu
Summary: Complex assessment of scaffold morphology and stiffness is important for bone filler development. The stiffness of the scaffold changes due to cell proliferation, but there are limitations.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE
(2022)
Article
Virology
Ivana Krizova, Alzbeta Dostalkova, Edison Castro, Jan Prchal, Romana Hadravova, Filip Kaufman, Richard Hrabal, Tomas Ruml, Manuel Llano, Luis Echegoyen, Michaela Rumlova
Summary: Fullerene derivatives with hydrophilic substituents have been shown to exhibit various biological activities, including antiviral effects. Recent studies suggest that the anti-HIV activity of fullerene derivatives may not be solely attributed to their binding to HIV-1 protease, but could involve a novel mechanism related to viral genomic RNA packaging and HIV-1 cDNA synthesis, potentially affecting HIV-1 infectivity. Additionally, the oxidative activity and fluorescence quenching of fullerene derivatives might contribute to the diversity of reported anti-HIV-1 mechanisms.
Review
Virology
Lucie Hozakova, Barbora Vokata, Tomas Ruml, Pavel Ulbrich
Summary: Various strategies have been developed to combat viral infections, including vaccines and antiviral compounds; The combination of multiple antiviral strategies or compounds is crucial in RNA virus research; Research on compounds targeting viral capsid proteins of RNA viruses is a rapidly evolving field.
Article
Medicine, Research & Experimental
Katerina Holasova, Bara Krizkovska, Lan Hoang, Simona Dobiasova, Jan Lipov, Tomas Macek, Vladimir Kren, Katerina Valentova, Tomas Ruml, Jitka Viktorova
Summary: Antibiotic resistance is a serious health problem, and the discovery of new antibiotics alone is no longer sufficient in combating multidrug-resistant infections. Adjuvant therapy and reducing bacterial virulence are gaining importance. Silymarin, a complex of flavonoids and flavonolignans, has a wide range of biological activities and can modulate drug resistance in cancer. This study tested eleven optically pure silymarin flavonolignans and found that some of them can reverse the multidrug resistance phenotype of Staphylococcus aureus and reduce its virulence.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Editorial Material
Virology
Tomas Ruml
Review
Agriculture, Multidisciplinary
Bara Krizkovska, Jitka Viktorova, Jan Lipov
Summary: Potatoes are a globally important crop, but their production and nutrient content are threatened by various stresses. Genetic engineering has been shown to be a valuable tool for improving pest resistance, reducing economic losses, and enhancing the nutritional properties of potatoes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Physical
Maria Zemkova, Peter Minarik, Eva Jablonska, Jozef Vesely, Jan Bohlen, Jiri Kubasek, Jan Lipov, Tomas Ruml, Vojtech Havlas, Robert Kral
Summary: This study investigates the use of Mg-3Y alloy as an alternative to the WE43 alloy. The results show that the omission of RE and Zr elements does not compromise the corrosion resistance and degradation rate of the W3 alloy compared to the WE43 alloy, while maintaining appropriate biocompatibility. It is also shown that the decrease in mechanical strength caused by the omission of RE and Zr can be compensated for by severe plastic deformation.
Article
Plant Sciences
Bara Krizkovska, Lan Hoang, Daniela Brdova, Kristyna Klementova, Nikoletta Szemeredi, Anna Louckova, Olga Kronusova, Gabriella Spengler, Petr Kastanek, Jana Hajslova, Jitka Viktorova, Jan Lipov
Summary: Salvia officinalis L., Sambucus nigra L., Matricaria chamomilla L., Agrimonia eupatoria L., Fragaria vesca L., and Malva sylvestris L. are plants that have been used in European folk medicine for treating skin wounds and infections. This study investigated their potential as adjuvants for the treatment of antibiotic-resistant bacteria. The results showed that Malva sylvestris had the highest antimicrobial activity, while Fragaria vesca and Sambucus nigra demonstrated potential as efflux pump inhibitors. Overall, these plants have potential for improving antibiotic treatment.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)