Article
Neurosciences
Mei Jiang, Hai-Tao Tu, Ke Zhang, Wei Zhang, Wei-Ping Yu, Jie Xu, Eng-King Tan, Kai-Hua Guo, Li Zeng
Summary: The VPS35 D620N mutation impairs adult hippocampal neurogenesis in transgenic mice, resulting in reduced neural stem cell pool, proliferation, and differentiation, as well as impaired migration and neurite outgrowth. Furthermore, the mutation hyperphosphorylates amyloid precursor protein (APP) and interacts with it, while loss of APP function rescues the inhibited neurogenesis, migration, and maturation caused by the VPS35 D620N mutation.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Cell Biology
Hyo-Jeong Yong, Jong-Ik Hwang, Jae-Young Seong
Summary: This study highlights the critical role of FAM19A1 in neuronal maturation and function by affecting dendritic spine development and neurite arborization, demonstrating its involvement in maintaining neural integrity and connectivity.
Article
Plant Sciences
Jin A. Jung, Heon Joo Lee, Myoung Chong Song, Areum Hwangbo, Ji Yoon Beom, Soo Jung Lee, Dong Jin Park, Ji Hoon Oh, Sang-Jun Ha, Eunji Cheong, Yeo Joon Yoon
Summary: Separating the immunosuppressive activity of FK506 from its neurotrophic activity, two new FK506 analogues were synthesized with a proline substitution, which significantly reduced immunosuppressive activity while retaining neurotrophic activity. This replacement is critical for enhancing neurotrophic activity while reducing strong immunosuppressive activity.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Ralf Kleene, Gabriele Loers, Melitta Schachner
Summary: Abnormal functions of L1 are associated with neural diseases. L1-55 fragment interacts with MeCP2, HP1, HistH1e, and other proteins via the KDET motif. These interactions regulate neurite outgrowth and neuronal survival, indicating the importance of L1 in neural system functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Giulia Puliatti, Domenica Donatella Li Puma, Giuseppe Aceto, Giacomo Lazzarino, Erica Acquarone, Renata Mangione, Luciano 'Adamio, Cristian Ripoli, Ottavio Arancio, Roberto Piacentini, Claudio Grassi
Summary: Our study found that the specific antibody anti-glypican 4 (GPC4) significantly reduced the uptake of extracellular tau oligomers (oTau) by astrocytes and prevented oTau-induced alterations in calcium-dependent gliotransmitter release. Furthermore, GPC4 expression was found to be APP/AICD-dependent and mediated the accumulation of oTau in astrocytes, resulting in synaptotoxic effects.
PROGRESS IN NEUROBIOLOGY
(2023)
Article
Clinical Neurology
Yinan Yao, Seong Su Kang, Yiyuan Xia, Zhi-Hao Wang, Xia Liu, Thorsten Muller, Yi E. Sun, Keqiang Ye
Summary: The truncated APP C586-695 fragment binds directly to the inflammatory transcription factor CEBPB, enhancing its transcriptional activity and exacerbating Alzheimer's disease-related gene expression and pathogenesis.
Article
Biochemistry & Molecular Biology
Benhong Xu, Chuanyue Gao, Huan Zhang, Xinfeng Huang, Xifei Yang, Chen Yang, Wei Liu, Desheng Wu, Jianjun Liu
Summary: The study revealed the important roles of PRDX3 in neurite outgrowth and Alzheimer's disease (AD) development. Knockdown of PRDX3 expression promoted retinoic acid-induced neurite outgrowth and led to dysregulation of multiple proteins.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Shufeng Bu, Quan Tang, Yan Wang, Samuel Song Yuan Lau, Wei Lin Yong, Fengwei Wu
Summary: In this study, the researchers identified the role of CLASP protein in dendrite pruning in fruit flies. They found that CLASP regulates microtubule orientation in dendrites and that the TOG2 domain is crucial for this function. The study also revealed that the Par-1 kinase acts as a suppressor of CLASP in dendritic microtubule orientation.
Article
Biochemistry & Molecular Biology
Alexander S. Haworth, Samantha L. Hodges, Alina L. Capatina, Lori L. Isom, Christoph G. Baumann, William J. Brackenbury
Summary: The beta 1 subunit of the voltage-gated Na+ channel plays a crucial role in alpha subunit function in breast cancer cells. It is cleaved by secretases, releasing extracellular and intracellular domains, and gamma-secretase may be important in breast cancer. The ICD of beta 1 is necessary and sufficient to increase Na+ current at the plasma membrane.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yu-pu Liu, Ming-yue Tian, Yi-duo Yang, Han Li, Tian-tian Zhao, Jin Zhu, Fang-fang Mou, Guo-hong Cui, Hai-dong Guo, Shui-jin Shao
Summary: This study found that the level of miR-21 was decreased in serum exosomes from DPN rats. Under high glucose conditions, Schwann cells (SC) proliferation was inhibited and cell apoptosis was increased. The expression of miR-21 in cells and exosomes was downregulated. miR-21 expression and SC-derived exosomes could affect neurite outgrowth through the AKT signaling pathway. This suggests the importance of SC-derived exosomal miR-21 in the regulation of neurite outgrowth under high glucose conditions.
Article
Engineering, Biomedical
Ho-Yin Yuen, Wai-Sze Yip, Suet To, Xin Zhao
Summary: Tissue curvature is a crucial anatomical parameter that affects cell behaviors. This study demonstrates that microcurvature substrates can significantly enhance neural polarity and differentiation, leading to various improvements in neural markers and properties. These findings provide new insights into the design of biomaterials for neural tissue engineering.
BIO-DESIGN AND MANUFACTURING
(2023)
Article
Biochemistry & Molecular Biology
Jinbae Yu, Youngsik Woo, Heesun Kim, Sihyeon An, Sang Ki Park, Sung Key Jang
Summary: FMRP is a multifunctional protein encoded by the FMR1 gene, and its deficiency is associated with fragile X syndrome. We discovered that FMRP controls neuronal development by enhancing the translation of 4EBP2, a key downstream regulator. This finding contributes to our understanding of the pathology of fragile X syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Hayley D. D. Ackerman, Glenn S. S. Gerhard
Summary: Increasing evidence supports the neuroprotective role of bile acids in major neurodegenerative disorders. The study focused on the two cellular receptors, FXR and TGR5, and their potential as neurotrophic agents. The results showed that DCA and TGR5 agonism could stimulate neurite outgrowth, suggesting a targetable pathway for promoting neurite growth.
Article
Chemistry, Medicinal
Kai-Cheng Hsu, Jung-Chun Chu, Hui-Ju Tseng, Chia- Liu, Hao-Ching Wang, Tony Eight Lin, Hong-Sheng Lee, Ling-Wei Hsin, Andrew H-J Wang, Chien-Huang Lin, Wei-Jan Huang
Summary: The study showed that modifying the acridine ring with phenothiazine derivatives can effectively inhibit the activity of class II HDACs, with compound 4f exhibiting the strongest inhibitory effect and promoting neurite outgrowth.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Svenja Koenig, Nadine Schmidt, Karin Bechberger, Sarah Morris, Mercedes Priego, Hannah Zaky, Yuyu Song, Jan Pielage, Silke Brunholz, Scott T. Brady, Stefan Kins, Gerardo Morfini
Summary: This study evaluated the intracellular effects of the amyloid precursor protein (APP) intracellular domain (AICD) on axonal transport. The results showed that AICD inhibits fast axonal transport (FAT), which is a deficit observed in neurons affected by Alzheimer's disease (AD). Further experiments revealed that this inhibitory effect is related to aberrant activation of axonal kinases and phosphorylation of the anterograde motor protein conventional kinesin. Pharmacological inhibitors of these kinases alleviated the inhibitory effect of AICD on FAT. Deletion experiments indicated that this effect requires a sequence encompassing the NPTY motif in AICD and interacting axonal proteins containing a phosphotyrosine-binding domain. These findings provide evidence for axon-specific effects of AICD and suggest a potential mechanistic framework linking alterations in APP processing, FAT deficits, and axonal pathology in AD.
Article
Oncology
Sijia Liu, Roman Gonzalez-Prieto, Mengdi Zhang, Paul P. Geurink, Raymond Kooij, Prasanna Vasudevan Iyengar, Maarten van Dinther, Erik Bos, Xiaobing Zhang, Sylvia E. Le Devedec, Bob van de Water, Roman I. Koning, Hong-Jian Zhu, Wilma E. Mesker, Alfred C. O. Vertegaal, Huib Ovaa, Long Zhang, John W. M. Martens, Peter ten Dijke
CLINICAL CANCER RESEARCH
(2020)
Review
Immunology
Xiaoxue Zhou, Feng Xie, Lin Wang, Long Zhang, Suping Zhang, Meiyu Fang, Fangfang Zhou
CELLULAR & MOLECULAR IMMUNOLOGY
(2020)
Review
Oncology
Jun Cao, Mengdi Zhang, Feng Xie, Jianlin Lou, Xiaoxue Zhou, Long Zhang, Meiyu Fang, Fangfang Zhou
Article
Biochemistry & Molecular Biology
Zhengkui Zhang, Xiuwu Fang, Xiaojin Wu, Li Ling, Feng Chu, Jingxian Li, Shuai Wang, Jia Zang, Bo Zhang, Sheng Ye, Long Zhang, Bing Yang, Shixian Lin, Huizhe Huang, Aijun Wang, Fangfang Zhou
Review
Chemistry, Multidisciplinary
Yetian Dong, Tong Dai, Jun Liu, Long Zhang, Fangfang Zhou
Review
Biochemistry & Molecular Biology
Tong Li, Bowen Wu, Tao Yang, Long Zhang, Ke Jin
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2020)
Article
Oncology
Jun Cao, Mengdi Zhang, Long Zhang, Jianlin Lou, Fangfang Zhou, Meiyu Fang
Summary: Thyroid cancer is the most common endocrine malignant tumor, with significantly increased incidence in recent years. Abnormal expression patterns of noncoding RNAs have been found in human thyroid cancer. Short ncRNAs, particularly miRNAs, have been extensively studied, while long ncRNAs, especially in human cancers like thyroid cancer, lack sufficient research.
Review
Chemistry, Multidisciplinary
Zhi Zong, Zhengkui Zhang, Liming Wu, Long Zhang, Fangfang Zhou
Summary: Innate antiviral immunity is the first line of defense against viral pathogens, relying on pattern recognition receptors to recognize pathogen-associated molecular patterns and initiate signaling cascades producing proinflammatory cytokines and IFN-I. The ubiquitin system, specifically DUBs, plays crucial roles in regulating immune responses, with viral DUBs counteracting host immunity. The potential of utilizing DUBs as therapeutic targets for infectious diseases is discussed, along with the challenges.
Review
Pharmacology & Pharmacy
Qinghui Zheng, Mengdi Zhang, Fangfang Zhou, Long Zhang, Xuli Meng
Summary: Drug resistance in breast cancer is a major challenge, with breast cancer stem cells (BCSCs) being responsible for it. Eliminating BCSCs can reverse drug resistance and improve treatment efficacy. Understanding the proliferation and resistance mechanisms of BCSCs is crucial in breast cancer therapy.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Humberto Sanchez, Kaley McCluskey, Theo van Laar, Edo van Veen, Filip M. Asscher, Belen Solano, John F. X. Diffley, Nynke H. Dekker
Summary: The study demonstrates the dynamics of the origin recognition complex and subsequent intermediates leading to the loading of an MCM helicase onto DNA using single-molecule optical trapping and confocal microscopy. ORC is shown to be a mobile protein that diffuses rapidly along DNA, with recruitment of MCM molecules in a ORC- and Cdc6-dependent way resulting in slow-moving ORC-MCM intermediates and rapidly scanning MCMs on DNA.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Daniel Ramirez Montero, Humberto Sanchez, Edo van Veen, Theo van Laar, Belen Solano, John F. X. Diffley, Nynke H. Dekker
Summary: In this study, the authors examined the single-molecule motion of fully reconstituted and activated CMG helicases. They found that CMG can move on DNA through both unidirectional translocation and diffusion, with a preference for the former in the presence of ATP and the latter in the absence of ATP. They also demonstrated that nucleotide binding can halt the diffusive motion of CMG. These findings support a mechanism in which nucleotide binding allows newly assembled CMG to engage with the DNA, halting diffusion and facilitating DNA melting for replication initiation.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Humberto Sanchez, Zhaowei Liu, Edo van Veen, Theo van Laar, John F. X. Diffley, Nynke H. Dekker
Summary: The study investigates the impact of chromatinizing origins of replication on ORC and MCM at the single-molecule level. The mobility of ORC is reduced, but its binding to the origin remains unchanged. MCM is efficiently recruited and spatially confined to the origin.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Yetian Dong, Tong Dai, Yujun Wei, Long Zhang, Min Zheng, Fangfang Zhou
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2020)
Review
Biochemistry & Molecular Biology
Zhangliang Cheng, Tong Dai, Xuelin He, Zhengkui Zhang, Feng Xie, Shuai Wang, Long Zhang, Fangfang Zhou
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2020)
Article
Cell Biology
Yihao Li, Chao Cui, Feng Xie, Szymon Kielbasa, Hailiang Mei, Maarten van Dinther, Hans van Dam, Andreas Bauer, Long Zhang, Peter ten Dijke
JOURNAL OF MOLECULAR CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
